Vision restoration presents a considerable challenge in the realm of regenerative medicine,while recent progress in ultrasound stimulation has displayed potential as a non-invasive therapeutic approach.This narrative ...Vision restoration presents a considerable challenge in the realm of regenerative medicine,while recent progress in ultrasound stimulation has displayed potential as a non-invasive therapeutic approach.This narrative review offers a comprehensive overview of current research on ultrasound-stimulated neuromodulation,emphasizing its potential as a treatment modality for various nerve injuries.By examining of the efficacy of different types of ultrasound stimulation in modulating peripheral and optic nerves,we can delve into their underlying molecular mechanisms.Furthermore,the review underscores the potential of sonogenetics in vision restoration,which involves leveraging pharmacological and genetic manipulations to inhibit or enhance the expression of related mechanosensitive channels,thereby modulating the strength of the ultrasound response.We also address how methods such as viral transcription can be utilized to render specific neurons or organs highly responsive to ultrasound,leading to significantly improved therapeutic outcomes.展开更多
Recent advancements in biomedical research have underscored the importance of noninvasive cellular manipulation techniques.Sonogenetics,a method that uses genetic engineering to produce ultrasound-sensitive proteins i...Recent advancements in biomedical research have underscored the importance of noninvasive cellular manipulation techniques.Sonogenetics,a method that uses genetic engineering to produce ultrasound-sensitive proteins in target cells,is gaining prominence along with optogenetics,electrogenetics,and magnetogenetics.Upon stimulation with ultrasound,these proteins trigger a cascade of cellular activities and functions.Unlike traditional ultrasound modalities,sonogenetics offers enhanced spatial selectivity,improving precision and safety in disease treatment.This technology broadens the scope of non-surgical interventions across a wide range of clinical research and therapeutic applications,including neuromodulation,oncologic treatments,stem cell therapy,and beyond.Although current literature predominantly emphasizes ultrasonic neuromodulation,this review offers a comprehensive exploration of sonogenetics.We discuss ultrasound properties,the specific ultrasound-sensitive proteins employed in sonogenetics,and the technique’s potential in managing conditions such as neurological disorders,cancer,and ophthalmic diseases,and in stem cell therapies.Our objective is to stimulate fresh perspectives for further research in this promising field.展开更多
In a recent study published in Cell,Liu et al.1 introduced EchoBack-CAR,a sonogenetic chimeric antigen receptor T cell platform that enables remote,spatiotemporal activation of CAR expression using focused ultrasound(...In a recent study published in Cell,Liu et al.1 introduced EchoBack-CAR,a sonogenetic chimeric antigen receptor T cell platform that enables remote,spatiotemporal activation of CAR expression using focused ultrasound(FUS).By combining an evolved heatinducible promoter with a synthetic positive feedback loop,EchoBack-CAR achieves potent antitumor effects while minimizing off-tumor toxicity.This system offers a promising solution for treating solid tumors,which have remained a significant challenge for conventional CAR-T therapies(Fig.1a,b).展开更多
基金supported by R01EY032229(Biomechanical Mapping of the Optic Nerve Head and Peripapillary Sclera Using High Frequency Ultrasonic Elastography)R01EY028662(High-resolution Elastographic Assessment of the Optic Nerve Head)+2 种基金R01EY030126(Non-invasive Ultrasound Stimulated Retinal Prosthesis)(to QZ)Unrestricted Grant to the Department of Ophthalmology from Research to Prevent Blindness,New York,NY(to MSH)the National Eye Institute of the National Institutes of Health under Award Number P30EY029220(to MSH)。
文摘Vision restoration presents a considerable challenge in the realm of regenerative medicine,while recent progress in ultrasound stimulation has displayed potential as a non-invasive therapeutic approach.This narrative review offers a comprehensive overview of current research on ultrasound-stimulated neuromodulation,emphasizing its potential as a treatment modality for various nerve injuries.By examining of the efficacy of different types of ultrasound stimulation in modulating peripheral and optic nerves,we can delve into their underlying molecular mechanisms.Furthermore,the review underscores the potential of sonogenetics in vision restoration,which involves leveraging pharmacological and genetic manipulations to inhibit or enhance the expression of related mechanosensitive channels,thereby modulating the strength of the ultrasound response.We also address how methods such as viral transcription can be utilized to render specific neurons or organs highly responsive to ultrasound,leading to significantly improved therapeutic outcomes.
基金the National Natural Science Foundation of China(No.81771845)the Chongqing Science and Technology Committee,Chongqing,China(No.CSTB2022NSCQ-MSX0812).
文摘Recent advancements in biomedical research have underscored the importance of noninvasive cellular manipulation techniques.Sonogenetics,a method that uses genetic engineering to produce ultrasound-sensitive proteins in target cells,is gaining prominence along with optogenetics,electrogenetics,and magnetogenetics.Upon stimulation with ultrasound,these proteins trigger a cascade of cellular activities and functions.Unlike traditional ultrasound modalities,sonogenetics offers enhanced spatial selectivity,improving precision and safety in disease treatment.This technology broadens the scope of non-surgical interventions across a wide range of clinical research and therapeutic applications,including neuromodulation,oncologic treatments,stem cell therapy,and beyond.Although current literature predominantly emphasizes ultrasonic neuromodulation,this review offers a comprehensive exploration of sonogenetics.We discuss ultrasound properties,the specific ultrasound-sensitive proteins employed in sonogenetics,and the technique’s potential in managing conditions such as neurological disorders,cancer,and ophthalmic diseases,and in stem cell therapies.Our objective is to stimulate fresh perspectives for further research in this promising field.
基金supported by National Research Foundation of Korea(NRF)grants funded by the Korean government(MSIT)(RS-2023-00279771 and RS-2024-00400827).
文摘In a recent study published in Cell,Liu et al.1 introduced EchoBack-CAR,a sonogenetic chimeric antigen receptor T cell platform that enables remote,spatiotemporal activation of CAR expression using focused ultrasound(FUS).By combining an evolved heatinducible promoter with a synthetic positive feedback loop,EchoBack-CAR achieves potent antitumor effects while minimizing off-tumor toxicity.This system offers a promising solution for treating solid tumors,which have remained a significant challenge for conventional CAR-T therapies(Fig.1a,b).
