Sonodynamic therapy(SDT)is garnering considerable attention as a promising treatment for deep-seated tumors because of its strong tissue penetration ability,non-invasiveness,and controllability.However,the SDT efficie...Sonodynamic therapy(SDT)is garnering considerable attention as a promising treatment for deep-seated tumors because of its strong tissue penetration ability,non-invasiveness,and controllability.However,the SDT efficiency of traditional sonosensitizers including porphyrins and their derivatives are limited due to their poor water dissolubility,high aggregation,and low reactive oxygen species(ROS)production efficiency.Consequently,it is crucial to develop novel sonosensitizers with high yields of ROS,outstanding water solubility,and good biocompatibility.Herein,we constructed a new platform for SDT based on unimolecular porphyrin derivatives OPV-C_(3)-TPP.The probe OPV-C_(3)-TPP was synthesized by covalently linking conjugated oligomers(OPV)with 5,10,15,20-tetra(4-aminophenyl)porphyrin(TAPP).The introduction of OPV greatly improves the water solubility of the porphyrins and reduces the self-aggregation of the porphyrins.In addition,OPV-C_(3)-TPP has good intramolecular energy transfer efficiency,thus enhancing the yield of ROS.The experimental results show that OPV-C_(3)-TPP exhibits excellent ROS generation capacity under ultrasound(US)irradiation,which leads to apoptosis and necrosis of tumor cells.In vivo tumor growth is also significantly inhibited in the OPV-C_(3)-TPP t US group,exhibiting better SDT effects than TAPP.Therefore,the unimolecular OPV-C_(3)-TPP can be used as a potential sonosensitizer,providing a promising SDT for deep-tissue tumors.展开更多
Sonodynamic therapy(SDT)as an emerging modality for malignant tumors mainly involves in sonosensitizers and low-intensity ultrasound(US),which can safely penetrate the tissue without significant attenuation.SDT not on...Sonodynamic therapy(SDT)as an emerging modality for malignant tumors mainly involves in sonosensitizers and low-intensity ultrasound(US),which can safely penetrate the tissue without significant attenuation.SDT not only has the advantages including high precision,non-invasiveness,and minimal side effects,but also overcomes the limitation of low penetration of light to deep tumors.The cytotoxic reactive oxygen species can be produced by the utilization of sonosensitizers combined with US and kill tumor cells.However,the underlying mechanism of SDT has not been elucidated,and its unsatisfactory efficiency retards its further clinical application.Herein,we shed light on the main mechanisms of SDT and the types of sonosensitizers,including organic sonosensitizers and inorganic sonosensitizers.Due to the development of nanotechnology,many novel nanoplatforms are utilized in this arisen field to solve the barriers of sonosensitizers and enable continuous innovation.This review also highlights the potential advantages of nanosonosensitizers and focus on the enhanced efficiency of SDT based on nanosonosensitizers with monotherapy or synergistic therapy for deep tumors that are difficult to reach by traditional treatment,especially orthotopic cancers.展开更多
Sonodynamic therapy(SDT)is a new non-invasive treatment method,which uses low-intensity ultrasound(US)to activate specific sonosensitizers(SNs)to produce reactive oxygen species(ROS)for therapeutic purposes.However,tr...Sonodynamic therapy(SDT)is a new non-invasive treatment method,which uses low-intensity ultrasound(US)to activate specific sonosensitizers(SNs)to produce reactive oxygen species(ROS)for therapeutic purposes.However,traditional sonosensitizers have the defects of low generation efficiency of ROS and single treatment mode.Therefore,designing sonosensitizers with high efficiency to generate ROS,high stability,and multimodal therapy is an excellent alternative to achieve effective,safe,and intelligent therapy.Heterojunction nanosonosensitizers(NSNs),as novel type of SNs,combine different materials through heterojunction structures to improve the efficiency of ROS generation.In this review,the classification of heterojunction NSNs,the preparation methods and characterization methods of heterojunction NSNs and the possible mechanisms for enhancing SDT were firstly presented,followed by an in-depth discussion of the application of heterojunction NSNs in the treatment of bacterial infections and tumors,with a special emphasis on synergistic enhancement of therapeutic efficacy of heterojunction SNs in combination with different therapeutic models such as gas therapy,immunotherapy and nanocatalytic therapy.Finally,the challenges and perspectives of such heterojunction SNs-supported SDT were outlined and highlighted to facilitate their clinical translation.展开更多
Conventional antibiotic treatment of bacterial infections associated with biofilms usually suffers from poor penetration and drug resistance.Ultrasound(US)-responsive antibacterial systems have shown great promise in ...Conventional antibiotic treatment of bacterial infections associated with biofilms usually suffers from poor penetration and drug resistance.Ultrasound(US)-responsive antibacterial systems have shown great promise in the elimination of bacterial biofilms,benefiting from their unique sonophysical and sonochemical effects.In this study,PFP@Lip-BNN6/Ce6 nanodroplets(PLBC NDs)were prepared by using perfluoropentane(PFP)to load chlorin e6(Ce6)and a nitric oxide(NO)precursor(BNN6)for treating Staphylococcus aureus(S.aureus)implant infection.PLBC NDs physically disrupt the biofilm structure by US-triggered PFP phase transition and cavitation to enhance the permeation of Ce6 and BNN6.Under US irradiation,Ce6 generates various reactive oxygen species(ROS),such as singlet oxygen(1O2)and superoxide anion(O_(2)^(•-));BNN6 releases NO and then reacts with O_(2)^(•-)to form peroxynitrite anion(ONOO^(-)),one of the long-lived reactive nitrogen species(RNS),thus realizing synergistic ROS/RNS antibacterial activity.In vitro experiments showed that PLBC NDs reduced the biofilm biomass of S.aureus in 96-well plates by 65.9%,with a bacterial inactivation rate of 4.4 log(99.995%),significantly surpassing single treatments.Transcriptomic analysis indicated that PLBC NDs can interfere with key pathways of S.aureus biosynthesis,metabolism,and oxidative stress.In a mouse titanium implant infection model,PLBC NDs reduced the number of viable bacteria in infected tissues by 3.5 log(99.97%)and promoted macrophage polarization towards an antiinflammatory phenotype(M2).Toxicity assessments demonstrated the favorable safety profile of PLBC NDs.This study presents a multifunctional US-responsive nanoplatform integrating sonophysical disruption and sonochemical killing for effective biofilm infection treatment.展开更多
Although sonodynamic therapy(SDT)is a promising cancer treatment that induces DNA and macromolecular damage through the generation of reactive oxygen species(ROS),its therapeutic efficacy is limited by local hypoxia a...Although sonodynamic therapy(SDT)is a promising cancer treatment that induces DNA and macromolecular damage through the generation of reactive oxygen species(ROS),its therapeutic efficacy is limited by local hypoxia and ROS defense mechanisms in tumors.This study propose d a novel tumor treatment approach,focusing on ROS-mediated therapy by targ eting the nucleus and depleting glutathione(GSH)levels,which was achieved through a nanoplatform(Pt^(2+)-CDs@PpIX)with integrated functions including GSH detection and depletion,pH-responsive drug release,and nuclear targeting.The Pt^(2+)-CDs@PpIX nanoplatform effectively differentiated normal and cancer cells and also exhibited excellent biocompatibility.Depletion of GSH levels and increased ROS sensitivity of cells significantly improved the effectiveness of SDT,as demonstrated in vitro using Pt^(2+)-CDs@PpIX,which also exhibited significant cellular uptake.Pt^(2+)-CDs@PpIX exerted potent antitumor effects in both two-dimensional and three-dimensional tum or microenvironment models(3 DM-7721).Moreover,in 3 DM-7721 models,hepatoma cells(SMMC-7721)demonstrated significant inhibition of motility,invasion,and colony formation after exposure to Pt^(2+)-CDs@PpIX.Furthermore,intravenous administration of the Pt^(2+)-CDs@PpIX nanoplatform enabled precise and rapid tumor-targeting,followed by ultrasound-triggered therapy,without adverse effects in nude mice.Hence,this nanoplatform provides a promising strategy for designing cancer therapies and delivering nuclear-targeted drugs.展开更多
The hydrophobic sonosensitizer IR780 iodide(IR780)was loaded into liposomes to form Liposome@IR780 nanoparticles(NPs)for triple-negative breast cancer(TNBC)to enhance SDT via low-intensity ultrasound(LIU)irradiation.T...The hydrophobic sonosensitizer IR780 iodide(IR780)was loaded into liposomes to form Liposome@IR780 nanoparticles(NPs)for triple-negative breast cancer(TNBC)to enhance SDT via low-intensity ultrasound(LIU)irradiation.The NPs were characterized using various physicochemical methods including size distribution,zeta potential,and morphology.In vitro experiments show that the Liposome@IR780 NPs can generate more reactive oxygen species(ROS)upon LIU irradiation.The apoptosis experiment results further demonstrate that Liposome@IR780 NPs show better apoptosis rate against 4T1 cells.Our results indicate that Liposome@IR780 NPs will provide a promising approach for TNBC upon SDT treatment.展开更多
Pancreatic cancer is highly vulnerable to ferroptosis.Consequently,treatments that target pancreatic cancer through ferroptosis induction demonstrate immense potential for enhancing therapeutic outcomes in this condit...Pancreatic cancer is highly vulnerable to ferroptosis.Consequently,treatments that target pancreatic cancer through ferroptosis induction demonstrate immense potential for enhancing therapeutic outcomes in this condition.In the present study,we synthesized hollow mesoporous iron oxide nanoparticles(MHFe)using a hydrothermal method.These nanoparticles retained the superparamagnetic properties of iron oxide and its Fenton reaction-catalyzing ability.Meanwhile,they also showed superior drug-loading capacity compared to traditional ferric oxide nanoparticles due to their hollow and mesoporous structure.Under the guidance of a magnetic field,these nanoparticles could accumulate in tumor cells.Following the incorporation of Ce6,a sonosensitizer,the Ce6@MHFe system could generate singlet oxygen under ultrasound treatment to promote tumor cell apoptosis while simultaneously producing hydroxyl radicals through the enhanced Fenton effect of MHFe.This promoted ferroptosis in pancreatic cancer cells,achieving combined therapeutic effects.In vivo experiments confirmed the good biocompatibility of Ce6@MHFe and demonstrated that the nanoparticles could effectively kill tumor cells under magnetic targeting and ultrasound irradiation,thereby inhibiting tumor growth.The findings suggested that these hollow mesoporous iron oxide nanoparticles(Ce6@MHFe)with a high drug-loading capacity,tumor retention ability,and potential for combination therapy have potential for the treatment of various malignancies,including pancreatic cancer.展开更多
A decomposable and sono-enzyme co-triggered nanoparticle(p TCP-CR NP)with“AND gate”logic was synthesized,combining a meso–carboxyl-porphyrin-based sonosensitizer(5,10,15,20-tetrakis(carboxyl)porphyrin,TCP)and a thi...A decomposable and sono-enzyme co-triggered nanoparticle(p TCP-CR NP)with“AND gate”logic was synthesized,combining a meso–carboxyl-porphyrin-based sonosensitizer(5,10,15,20-tetrakis(carboxyl)porphyrin,TCP)and a thiophenyl-croconium(2,5-bis[(2-(2-(2-hydroxyethoxy)ethoxy)ethyl-4-carboxylate-piperidylamino)thiophenyl]-croconium,CR)via ester groups.TCP releases carbon monoxide(CO)under ultrasound(US)irradiation,offering both sonodynamic and gas therapy.CR decomposes into stronger reactive oxygen species(ROS)compared to oxygen-based radicals.The F?rster resonance energy transfer(FRET)effect between TCP and CR inhibits ROS and CO generation until triggered by tumor cell overexpressed carboxylesterase(CEs).p TCP-CR NPs“AND gate”logic ensures activation only in the presence of both CEs and US,targeting tumor cells while safety in normal tissues.The ROS and CO generation abilities,as well as the releasing of SO_(4)^(·-)have been systemically examined.p TCP-CR can be thoroughly decomposed into low-toxic molecules post the treatment,showing the safety with negligible phototoxic reactions.In vivo anti-cancer therapy has been evaluated using mice bearing hepatocellular carcinoma.展开更多
Sonodynamic therapy(SDT) is an emerging approach that involves a combination of low-intensity ultrasound and specialized chemical agents known as sonosensitizers. Ultrasound can penetrate deeply into tissues and can b...Sonodynamic therapy(SDT) is an emerging approach that involves a combination of low-intensity ultrasound and specialized chemical agents known as sonosensitizers. Ultrasound can penetrate deeply into tissues and can be focused into a small region of a tumor to activate a sonosensitizer which offers the possibility of non-invasively eradicating solid tumors in a site-directed manner.In this article, we critically reviewed the currently accepted mechanisms of sonodynamic action and summarized the classification of sonosensitizers. At the same time, the breath of evidence from SDT-based studies suggests that SDT is promising for cancer treatment.展开更多
Ultrasound can be used not only in examination, but also in therapy, especially in the therapy of cancer. Sonodynamic therapy is an experimental cancer therapy method which uses ultrasound to enhance the cytotoxic eff...Ultrasound can be used not only in examination, but also in therapy, especially in the therapy of cancer. Sonodynamic therapy is an experimental cancer therapy method which uses ultrasound to enhance the cytotoxic effects of agents known as sonosensitizers. It has been tested in vitro and in vivo. The ultrasound could penetrate the tissue and cell under some of conditions which directly changes cell membrane permeability, thereby allowing the delivery of exogenous molecules into the cells in some degree. Ultrasound could inhibit the proliferation or induce the apoptosis of cancer cells in vitro or in vivo. Recent researches indicated low-frequency and low-intensity ultrasound could induce cell apoptosis, which could be strengthened by sonodynamic sensitivity, microbubbles, chemotherapeutic drugs and so on. Most kinds of ultrasound suppressed the proliferation of cancer cells through inducing the apoptosis of cancer cells. The mechanism of apoptosis is not clear. In this review, we will focus on and discuss the mechanisms of the induction of cancer cell apoptosis by ultrasound.展开更多
Rapid evolution and propagation of multidrug resistance among bacterial pathogens are outpacing the development of new antibiotics,but antimicrobial photodynamic therapy(aPDT)provides an excellent alternative.This tre...Rapid evolution and propagation of multidrug resistance among bacterial pathogens are outpacing the development of new antibiotics,but antimicrobial photodynamic therapy(aPDT)provides an excellent alternative.This treatment depends on the interaction between light and photoactivated sensitizer to generate reactive oxygen species(ROS),which are highly cytotoxic to induce apoptosis in virtually all microorganisms without resistance concern.When replacing light with low-frequency ultrasonic wave to activate sensitizer,a novel ultrasounddriven treatment emerges as antimicrobial sonodynamic therapy(aSDT).Recent advances in aPDT and aSDT reveal golden opportunities for the management of multidrug resistant bacterial infections,especially in the theranostic application where imaging diagnosis can be accomplished facilely with the inherent optical characteristics of sensitizers,and the generated ROS by aPDT/SDT cause broad-spectrum oxidative damage for sterilization.In this review,we systemically outline the mechanisms,targets,and current progress of aPDT/SDT for bacterial theranostic application.Furthermore,potential limitations and future perspectives are also highlighted.展开更多
Drug resistance is considered the most important reason for the clinical failure of cancer chemotherapy.Circumventing drug resistance and improving the efficacy of anticancer agents remains a major challenge.Over the ...Drug resistance is considered the most important reason for the clinical failure of cancer chemotherapy.Circumventing drug resistance and improving the efficacy of anticancer agents remains a major challenge.Over the past several decades,photodynamic therapy(PDT)and sonodynamic therapy(SDT)have attracted substantial attention for their efficacy in cancer treatment,and have been combined with chemotherapy to overcome drug resistance.However,simultaneously delivering sensitizers and chemotherapy drugs to same tumor cell remains challenging,thus greatly limiting this combinational therapy.The rapid development of nanotechnology provides a new approach to solve this problem.Nano-based drug delivery systems can not only improve the targeted delivery of agents but also co-deliver multiple drug components in single nanoparticles to achieve optimal synergistic effects.In this review,we briefly summarize the mechanisms of drug resistance,discuss the advantages and disadvantages of PDT and SDT in reversing drug resistance,and describe state-of-the-art research using nano-mediated PDT and SDT to solve these refractory problems.This review also highlights the clinical translational potential for this combinational therapy.展开更多
Glioblastoma multiforme(GBM) is the most common primary malignant brain tumor, and it is associated with poor prognosis. Its characteristics of being highly invasive and undergoing heterogeneous genetic mutation, as w...Glioblastoma multiforme(GBM) is the most common primary malignant brain tumor, and it is associated with poor prognosis. Its characteristics of being highly invasive and undergoing heterogeneous genetic mutation, as well as the presence of the blood–brain barrier(BBB), have reduced the efficacy of GBM treatment. The emergence of a novel therapeutic method, namely, sonodynamic therapy(SDT), provides a promising strategy for eradicating tumors via activated sonosensitizers coupled with low-intensity ultrasound. SDT can provide tumor killing effects for deep-seated tumors, such as brain tumors. However, conventional sonosensitizers cannot effectively reach the tumor region and kill additional tumor cells, especially brain tumor cells. Efforts should be made to develop a method to help therapeutic agents pass through the BBB and accumulate in brain tumors. With the development of novel multifunctional nanosensitizers and newly emerging combination strategies, the killing ability and selectivity of SDT have greatly improved and are accompanied with fewer side effects. In this review, we systematically summarize the findings of previous studies on SDT for GBM, with a focus on recent developments and promising directions for future research.展开更多
Compared with traditional photodynamic therapy(PDT),ultrasound(US)triggered sonodynamic therapy(SDT)has a wide application prospect in tumor therapy because of its deeper penetration depth.Herein,a novel MnSiO_(3)-Pt(...Compared with traditional photodynamic therapy(PDT),ultrasound(US)triggered sonodynamic therapy(SDT)has a wide application prospect in tumor therapy because of its deeper penetration depth.Herein,a novel MnSiO_(3)-Pt(MP)nanocomposite composed of Mn Si O_(3)nanosphere and noble metallic Pt was successfully constructed.After modification with bovine serum albumin(BSA)and chlorine e6(Ce6),the multifunctional nanoplatform Mn SiO_(3)-Pt@BSA-Ce6(MPBC)realized the magnetic resonance imaging(MRI)-guided synergetic SDT/chemodynamic therapy(CDT).In this nanoplatform,sonosensitizer Ce6 can generate singlet oxygen(^(1)O_(2))to kill cancer cells under US irradiation.Meanwhile,the loaded Pt has the ability to catalyze the decomposition of overexpressed hydrogen peroxide(H_(2)O_(2))in tumor microenvironment(TME)to produce oxygen(O_(2)),which can conquer tumor hypoxia and promote the SDT-induced^(1)O_(2)production.In addition,MP can degrade in mildly acidic and reductive TME,causing the release of Mn^(2+).The released Mn^(2+) not only can be used for MRI,but also can generate hydroxyl radical(^·OH)for CDT by Fenton-like reaction.The multifunctional nanoplatform MPBC has high biological safety and good anticancer effect,which displays the great latent capacity in biological application.展开更多
Atherosclerosis(AS)is a chronic inflammatory disease of large and medium-sized arteries that leads to ischemic heart disease,stroke,and peripheral vascular disease.Despite the current treatments,mortality and disabili...Atherosclerosis(AS)is a chronic inflammatory disease of large and medium-sized arteries that leads to ischemic heart disease,stroke,and peripheral vascular disease.Despite the current treatments,mortality and disability still remain high.Sonodynamic therapy(SDT),a non-invasive and localized methodology,has been developed as a promising new treatment for inhibiting atherosclerotic progression and stabilizing plaques.Promising progress has been made through cell and animal assays,as well as clinical trials.For example,the effect of SDT on apoptosis and autophagy of cells in AS,especially macrophages,and the concept of non-lethal SDT has also been proposed.In this review,we summarize the ultrasonic parameters and known sonosensitizers utilized in SDT for AS;we elaborate on SDT's therapeutic effects and mechanisms in terms of macrophages,T lymphocytes,neovascularization,smooth muscle cells,lipid,extracellular matrix and efferocytosis within plaques;additionally,we discuss the safety of SDT.A comprehensive summary of the confirmed effects of SDT on AS is conducted to establish a framework for future researchers.展开更多
In recent years,because of the growing desire to improve the noninvasiveness and safety of tumor treatments,sonodynamic therapy has gradually become a popular research topic.However,due to the complexity of the therap...In recent years,because of the growing desire to improve the noninvasiveness and safety of tumor treatments,sonodynamic therapy has gradually become a popular research topic.However,due to the complexity of the therapeutic process,the relevant mechanisms have not yet been fully elucidated.One of the widely accepted possibilities involves the effect of reactive oxygen species.In this review,the mechanism of reactive oxygen species production by sonodynamic therapy(SDT)and ways to enhance the sonodynamic production of reactive oxygen species are reviewed.Then,the clinical application and limitations of SDT are discussed.In conclusion,current research on sonodynamic therapy should focus on the development of sonosensitizers that efficiently produce active oxygen,exhibit biological safety,and promote the clinical transformation of sonodynamic therapy.展开更多
Deep-tissue solid cancer treatment has a poor prognosis,resulting in a very low 5-year patient survival rate.The primary challenges facing solid tumor therapies are accessibility,incomplete surgical removal of tumor t...Deep-tissue solid cancer treatment has a poor prognosis,resulting in a very low 5-year patient survival rate.The primary challenges facing solid tumor therapies are accessibility,incomplete surgical removal of tumor tissue,the resistance of the hypoxic and heterogeneous tumor microenvironment to chemotherapy and radiation,and suffering caused by off-target toxicities.Here,sonodynamic therapy(SDT)is an evolving therapeutic approach that uses low-intensity ultrasound to target deep-tissue solid tumors.The ability of ultrasound to deliver energy safely and precisely into small deep-tissue(>10 cm)volumes makes SDT more effective than conventional photodynamic therapy.While SDT is currently in phase 1/2 clinical trials for glioblastoma multiforme,its use for other solid cancer treatments,such as breast,pancreatic,liver,and prostate cancer,is still in the preclinical stage,with further investigation required to improve its therapeutic efficacy.This review,therefore,focuses on recent advances in SDT cancer treatments.We describe the interaction between ultrasound and sonosensitizer molecules and the associated energy transfer mechanism to malignant cells,which plays a central role in SDT-mediated cell death.Different sensitizers used in clinical and preclinical trials of various cancer treatments are listed,and the critical ultrasound parameters for SDT are reviewed.We also discuss approaches to improve the efficacies of these sonosensitizers,the role of the 3-dimensional spheroid in vitro investigations,ultrasound-controlled CAR-T cell and SDT-based multimodal therapy,and machine learning for sonosensitizer optimization,which could facilitate clinical translation of SDT.展开更多
OBJECTIVE To investigate the effect of sonodynamic action on biofilm-producing Enterococcus faecalis using hypocrellin B from a natural product Hypocrella bambuase as a sensitizer.METHODS The uptake of hypocrellin B i...OBJECTIVE To investigate the effect of sonodynamic action on biofilm-producing Enterococcus faecalis using hypocrellin B from a natural product Hypocrella bambuase as a sensitizer.METHODS The uptake of hypocrellin B in biofilm-producing Enterococcus faecalis was measured using the fluorescent analysis method.After the combined treatment of hypocrellin B and ultrasound,the growth of biofilm-producing Enterococcus faecalis was measured using the colony counting method,the membrane integrity was detected using a flow cytometric analysis with propidium iodide staining.Chromosomal DNA of bacteria was analysed using apulsed-field gel electrophoresis(PFGE).RESULTS The uptake of hypocrellin B in biofilm-producing Enterocuccus faecalis reached a high peak at the 20 min incubation.After the combined treatment of hypocrellin B,the growth of biofilm-producing Enterocuccus faecalis significantly descreased and membrane integrity was remarkably damaged.However,no remarkable change in bacterial DNA was found.CONCLUSION Sonodynamic action of hypocrellin B had significant killing activity on Enterocuccus faecalis.展开更多
Traditional cancer treatments have disadvantages of large trauma area and toxic side effects while killing cancer cells.Peptide-targeted sonodynamic therapy(SDT)can effectively improve specificity of cancer treatment ...Traditional cancer treatments have disadvantages of large trauma area and toxic side effects while killing cancer cells.Peptide-targeted sonodynamic therapy(SDT)can effectively improve specificity of cancer treatment and overcome the problem of low tissue penetration depth caused by a photo-driven therapy.Herein,we developed a porphyrin-based sonosensitizer with a water-soluble polymer as a biological carrier and a cRGD peptide for tumor targeting,which constituted a nano sonosensitizer(T-cRGD NPs)for fluorescence imaging-guided sonodynamic therapy.A comparable sonosensitizer(T-PEG NPs)without the targeting unit was also prepared for illustration of therapeutic performance.Attribute to the role of peptide targeting,T-cRGD NPs can accumulate and enter tumor cells for fluorescence imaging and show a superior SDT effect than T-PEG NPs in vitro.The imaging in vivo reveals that T-cRGD NPs can enrich in tumor tissues within 14 h with a good biocompatibility.展开更多
Metal-based compounds with excellent photo-physical properties show good photochemotherapeutic performance.But,low in-depth tissue penetration of light limits their effectivity for deeply buried tumors.Encouraged by t...Metal-based compounds with excellent photo-physical properties show good photochemotherapeutic performance.But,low in-depth tissue penetration of light limits their effectivity for deeply buried tumors.Encouraged by the sonosensitizing ability of the traditional organic photosensitizers,here,we developed AuNPs@Ir1 as a sonosensitizer by hybridizing an organometallic Ir(Ⅲ) complex(Ir1) with ultrasmall gold nanoparticles(AuNPs) for efficient tumor sonodynamic therapy(SDT) for the first time.AuNPs@Ir1 rapidly entered the cancer cells,produced ^(1)O_(2),and catalytically oxidized NADH to NAD;under ultrasound(US)irradiation,thus resulted in cancer cells oncosis.Because of efficient passive retention in tumors post intravenous injection,AuNPs@Ir1 further efficiently inhibited the growth of tumors in-vivo under US stimulation without long-term toxicity to other organs.Overall,this work presents the excellent US triggered in-vitro and in-vivo anticancer profile of the novel AuNPs@Ir1.It is expected to increase the scope of SDT for metal-based anticancer drugs.展开更多
基金supported by the National Natural Science Foundation of China(22274095 and 21974084)the Fundamental Research Funds for the Central Universities(GK202302004).
文摘Sonodynamic therapy(SDT)is garnering considerable attention as a promising treatment for deep-seated tumors because of its strong tissue penetration ability,non-invasiveness,and controllability.However,the SDT efficiency of traditional sonosensitizers including porphyrins and their derivatives are limited due to their poor water dissolubility,high aggregation,and low reactive oxygen species(ROS)production efficiency.Consequently,it is crucial to develop novel sonosensitizers with high yields of ROS,outstanding water solubility,and good biocompatibility.Herein,we constructed a new platform for SDT based on unimolecular porphyrin derivatives OPV-C_(3)-TPP.The probe OPV-C_(3)-TPP was synthesized by covalently linking conjugated oligomers(OPV)with 5,10,15,20-tetra(4-aminophenyl)porphyrin(TAPP).The introduction of OPV greatly improves the water solubility of the porphyrins and reduces the self-aggregation of the porphyrins.In addition,OPV-C_(3)-TPP has good intramolecular energy transfer efficiency,thus enhancing the yield of ROS.The experimental results show that OPV-C_(3)-TPP exhibits excellent ROS generation capacity under ultrasound(US)irradiation,which leads to apoptosis and necrosis of tumor cells.In vivo tumor growth is also significantly inhibited in the OPV-C_(3)-TPP t US group,exhibiting better SDT effects than TAPP.Therefore,the unimolecular OPV-C_(3)-TPP can be used as a potential sonosensitizer,providing a promising SDT for deep-tissue tumors.
基金supported by the National Natural Science Foundation of China(Nos.U23A20573,U23A20140,22072154,and 22377127)Hebei Natural Science Foundation(B2024208046 and H2022423314)+2 种基金Huang jin tai plan project of Hebei provincial department of education(HJZD202512)S&T Program of Hebei,the Youth innovation Promotion Association of Chinese Academy of Sciences(No.2021048)the Beijing Nova Program(No.20230484352).
文摘Sonodynamic therapy(SDT)as an emerging modality for malignant tumors mainly involves in sonosensitizers and low-intensity ultrasound(US),which can safely penetrate the tissue without significant attenuation.SDT not only has the advantages including high precision,non-invasiveness,and minimal side effects,but also overcomes the limitation of low penetration of light to deep tumors.The cytotoxic reactive oxygen species can be produced by the utilization of sonosensitizers combined with US and kill tumor cells.However,the underlying mechanism of SDT has not been elucidated,and its unsatisfactory efficiency retards its further clinical application.Herein,we shed light on the main mechanisms of SDT and the types of sonosensitizers,including organic sonosensitizers and inorganic sonosensitizers.Due to the development of nanotechnology,many novel nanoplatforms are utilized in this arisen field to solve the barriers of sonosensitizers and enable continuous innovation.This review also highlights the potential advantages of nanosonosensitizers and focus on the enhanced efficiency of SDT based on nanosonosensitizers with monotherapy or synergistic therapy for deep tumors that are difficult to reach by traditional treatment,especially orthotopic cancers.
基金supported by Key Research Project of the Educational Department of Liaoning Province,China(No.JYTZD2023139).
文摘Sonodynamic therapy(SDT)is a new non-invasive treatment method,which uses low-intensity ultrasound(US)to activate specific sonosensitizers(SNs)to produce reactive oxygen species(ROS)for therapeutic purposes.However,traditional sonosensitizers have the defects of low generation efficiency of ROS and single treatment mode.Therefore,designing sonosensitizers with high efficiency to generate ROS,high stability,and multimodal therapy is an excellent alternative to achieve effective,safe,and intelligent therapy.Heterojunction nanosonosensitizers(NSNs),as novel type of SNs,combine different materials through heterojunction structures to improve the efficiency of ROS generation.In this review,the classification of heterojunction NSNs,the preparation methods and characterization methods of heterojunction NSNs and the possible mechanisms for enhancing SDT were firstly presented,followed by an in-depth discussion of the application of heterojunction NSNs in the treatment of bacterial infections and tumors,with a special emphasis on synergistic enhancement of therapeutic efficacy of heterojunction SNs in combination with different therapeutic models such as gas therapy,immunotherapy and nanocatalytic therapy.Finally,the challenges and perspectives of such heterojunction SNs-supported SDT were outlined and highlighted to facilitate their clinical translation.
基金supported by the National Natural Science Foundation of China(Nos.22375101,and 62288102).
文摘Conventional antibiotic treatment of bacterial infections associated with biofilms usually suffers from poor penetration and drug resistance.Ultrasound(US)-responsive antibacterial systems have shown great promise in the elimination of bacterial biofilms,benefiting from their unique sonophysical and sonochemical effects.In this study,PFP@Lip-BNN6/Ce6 nanodroplets(PLBC NDs)were prepared by using perfluoropentane(PFP)to load chlorin e6(Ce6)and a nitric oxide(NO)precursor(BNN6)for treating Staphylococcus aureus(S.aureus)implant infection.PLBC NDs physically disrupt the biofilm structure by US-triggered PFP phase transition and cavitation to enhance the permeation of Ce6 and BNN6.Under US irradiation,Ce6 generates various reactive oxygen species(ROS),such as singlet oxygen(1O2)and superoxide anion(O_(2)^(•-));BNN6 releases NO and then reacts with O_(2)^(•-)to form peroxynitrite anion(ONOO^(-)),one of the long-lived reactive nitrogen species(RNS),thus realizing synergistic ROS/RNS antibacterial activity.In vitro experiments showed that PLBC NDs reduced the biofilm biomass of S.aureus in 96-well plates by 65.9%,with a bacterial inactivation rate of 4.4 log(99.995%),significantly surpassing single treatments.Transcriptomic analysis indicated that PLBC NDs can interfere with key pathways of S.aureus biosynthesis,metabolism,and oxidative stress.In a mouse titanium implant infection model,PLBC NDs reduced the number of viable bacteria in infected tissues by 3.5 log(99.97%)and promoted macrophage polarization towards an antiinflammatory phenotype(M2).Toxicity assessments demonstrated the favorable safety profile of PLBC NDs.This study presents a multifunctional US-responsive nanoplatform integrating sonophysical disruption and sonochemical killing for effective biofilm infection treatment.
基金supported by the National Natural Science Foundation of China(No.62031022)the Sha nxi Provincial Basic Research Project(Nos.202103021221006 and 20210302123040)+2 种基金the Scientific and Technological Innovation Programs of Higher Education Institutions in Shanxi(No.2021L044)the Key R&D Program of Shanxi Province(No.202302130501006)the Shanxi‒Zheda Institute of Advanced Materials and Chemical Engineering(No.2022SX-TD026).
文摘Although sonodynamic therapy(SDT)is a promising cancer treatment that induces DNA and macromolecular damage through the generation of reactive oxygen species(ROS),its therapeutic efficacy is limited by local hypoxia and ROS defense mechanisms in tumors.This study propose d a novel tumor treatment approach,focusing on ROS-mediated therapy by targ eting the nucleus and depleting glutathione(GSH)levels,which was achieved through a nanoplatform(Pt^(2+)-CDs@PpIX)with integrated functions including GSH detection and depletion,pH-responsive drug release,and nuclear targeting.The Pt^(2+)-CDs@PpIX nanoplatform effectively differentiated normal and cancer cells and also exhibited excellent biocompatibility.Depletion of GSH levels and increased ROS sensitivity of cells significantly improved the effectiveness of SDT,as demonstrated in vitro using Pt^(2+)-CDs@PpIX,which also exhibited significant cellular uptake.Pt^(2+)-CDs@PpIX exerted potent antitumor effects in both two-dimensional and three-dimensional tum or microenvironment models(3 DM-7721).Moreover,in 3 DM-7721 models,hepatoma cells(SMMC-7721)demonstrated significant inhibition of motility,invasion,and colony formation after exposure to Pt^(2+)-CDs@PpIX.Furthermore,intravenous administration of the Pt^(2+)-CDs@PpIX nanoplatform enabled precise and rapid tumor-targeting,followed by ultrasound-triggered therapy,without adverse effects in nude mice.Hence,this nanoplatform provides a promising strategy for designing cancer therapies and delivering nuclear-targeted drugs.
文摘The hydrophobic sonosensitizer IR780 iodide(IR780)was loaded into liposomes to form Liposome@IR780 nanoparticles(NPs)for triple-negative breast cancer(TNBC)to enhance SDT via low-intensity ultrasound(LIU)irradiation.The NPs were characterized using various physicochemical methods including size distribution,zeta potential,and morphology.In vitro experiments show that the Liposome@IR780 NPs can generate more reactive oxygen species(ROS)upon LIU irradiation.The apoptosis experiment results further demonstrate that Liposome@IR780 NPs show better apoptosis rate against 4T1 cells.Our results indicate that Liposome@IR780 NPs will provide a promising approach for TNBC upon SDT treatment.
基金financially supported by the National Natural Science Foundation of China(Nos.32201138,No.82302193)the Natural Science Foundation of Zhejiang Province(No.LQ24H180004)
文摘Pancreatic cancer is highly vulnerable to ferroptosis.Consequently,treatments that target pancreatic cancer through ferroptosis induction demonstrate immense potential for enhancing therapeutic outcomes in this condition.In the present study,we synthesized hollow mesoporous iron oxide nanoparticles(MHFe)using a hydrothermal method.These nanoparticles retained the superparamagnetic properties of iron oxide and its Fenton reaction-catalyzing ability.Meanwhile,they also showed superior drug-loading capacity compared to traditional ferric oxide nanoparticles due to their hollow and mesoporous structure.Under the guidance of a magnetic field,these nanoparticles could accumulate in tumor cells.Following the incorporation of Ce6,a sonosensitizer,the Ce6@MHFe system could generate singlet oxygen under ultrasound treatment to promote tumor cell apoptosis while simultaneously producing hydroxyl radicals through the enhanced Fenton effect of MHFe.This promoted ferroptosis in pancreatic cancer cells,achieving combined therapeutic effects.In vivo experiments confirmed the good biocompatibility of Ce6@MHFe and demonstrated that the nanoparticles could effectively kill tumor cells under magnetic targeting and ultrasound irradiation,thereby inhibiting tumor growth.The findings suggested that these hollow mesoporous iron oxide nanoparticles(Ce6@MHFe)with a high drug-loading capacity,tumor retention ability,and potential for combination therapy have potential for the treatment of various malignancies,including pancreatic cancer.
基金supported by grants from the National Natural Science Foundation of China(No.22375027)the Natural Science Foundation of Jiangsu Province(Nos.BK20221265,BK20211100)+1 种基金the Fundamental Research Funds for the Central Universities(No.DUT23YG133)the Research Funds from Liaoning Cancer Hospital(No.2024ZLKF-35)。
文摘A decomposable and sono-enzyme co-triggered nanoparticle(p TCP-CR NP)with“AND gate”logic was synthesized,combining a meso–carboxyl-porphyrin-based sonosensitizer(5,10,15,20-tetrakis(carboxyl)porphyrin,TCP)and a thiophenyl-croconium(2,5-bis[(2-(2-(2-hydroxyethoxy)ethoxy)ethyl-4-carboxylate-piperidylamino)thiophenyl]-croconium,CR)via ester groups.TCP releases carbon monoxide(CO)under ultrasound(US)irradiation,offering both sonodynamic and gas therapy.CR decomposes into stronger reactive oxygen species(ROS)compared to oxygen-based radicals.The F?rster resonance energy transfer(FRET)effect between TCP and CR inhibits ROS and CO generation until triggered by tumor cell overexpressed carboxylesterase(CEs).p TCP-CR NPs“AND gate”logic ensures activation only in the presence of both CEs and US,targeting tumor cells while safety in normal tissues.The ROS and CO generation abilities,as well as the releasing of SO_(4)^(·-)have been systemically examined.p TCP-CR can be thoroughly decomposed into low-toxic molecules post the treatment,showing the safety with negligible phototoxic reactions.In vivo anti-cancer therapy has been evaluated using mice bearing hepatocellular carcinoma.
基金supported by the National Natural Science Foundation of China(Grant No.81573005 and 81371671)
文摘Sonodynamic therapy(SDT) is an emerging approach that involves a combination of low-intensity ultrasound and specialized chemical agents known as sonosensitizers. Ultrasound can penetrate deeply into tissues and can be focused into a small region of a tumor to activate a sonosensitizer which offers the possibility of non-invasively eradicating solid tumors in a site-directed manner.In this article, we critically reviewed the currently accepted mechanisms of sonodynamic action and summarized the classification of sonosensitizers. At the same time, the breath of evidence from SDT-based studies suggests that SDT is promising for cancer treatment.
基金supported by the Major Infrastructure Projects of Shanghai Science and Technology (No. 10JC1412600)
文摘Ultrasound can be used not only in examination, but also in therapy, especially in the therapy of cancer. Sonodynamic therapy is an experimental cancer therapy method which uses ultrasound to enhance the cytotoxic effects of agents known as sonosensitizers. It has been tested in vitro and in vivo. The ultrasound could penetrate the tissue and cell under some of conditions which directly changes cell membrane permeability, thereby allowing the delivery of exogenous molecules into the cells in some degree. Ultrasound could inhibit the proliferation or induce the apoptosis of cancer cells in vitro or in vivo. Recent researches indicated low-frequency and low-intensity ultrasound could induce cell apoptosis, which could be strengthened by sonodynamic sensitivity, microbubbles, chemotherapeutic drugs and so on. Most kinds of ultrasound suppressed the proliferation of cancer cells through inducing the apoptosis of cancer cells. The mechanism of apoptosis is not clear. In this review, we will focus on and discuss the mechanisms of the induction of cancer cell apoptosis by ultrasound.
基金supported by the National Key Research and Development Program of China(2017YFA0205201 and 2016YFC0106900)the National Natural Science Foundation of China(81925019,81422023,81701752,81901808,and U1705281)+2 种基金the Fundamental Research Funds for the Central Universities(20720200019 and 20720190088)the Program for New Century Excellent Talents in University,China(No.NCET-13-0502)the China Postdoctoral Science Foundation(2019M662545)。
文摘Rapid evolution and propagation of multidrug resistance among bacterial pathogens are outpacing the development of new antibiotics,but antimicrobial photodynamic therapy(aPDT)provides an excellent alternative.This treatment depends on the interaction between light and photoactivated sensitizer to generate reactive oxygen species(ROS),which are highly cytotoxic to induce apoptosis in virtually all microorganisms without resistance concern.When replacing light with low-frequency ultrasonic wave to activate sensitizer,a novel ultrasounddriven treatment emerges as antimicrobial sonodynamic therapy(aSDT).Recent advances in aPDT and aSDT reveal golden opportunities for the management of multidrug resistant bacterial infections,especially in the theranostic application where imaging diagnosis can be accomplished facilely with the inherent optical characteristics of sensitizers,and the generated ROS by aPDT/SDT cause broad-spectrum oxidative damage for sterilization.In this review,we systemically outline the mechanisms,targets,and current progress of aPDT/SDT for bacterial theranostic application.Furthermore,potential limitations and future perspectives are also highlighted.
基金supported by grants from the National Key Research and Development Program of China(Grant No.2016YFA0201400)State Key Program of National Natural Science of China(Grant No.81930047)+4 种基金Projects of International Cooperation and Exchanges NSFC-PSF(Grant No.31961143003)National Project for Research and Development of Major Scientific Instruments(Grant No.81727803)Beijing Natural Science Foundation,Haidian,Original Innovation Joint Fund(Grant No.17 L20170)Foundation for Innovative Research Groups of the National Natural Science Foundation of China(Grant Nos.81421004 and 52003161)Shenzhen Science and Technology Project(Grant No.JCYJ20180507183842516)。
文摘Drug resistance is considered the most important reason for the clinical failure of cancer chemotherapy.Circumventing drug resistance and improving the efficacy of anticancer agents remains a major challenge.Over the past several decades,photodynamic therapy(PDT)and sonodynamic therapy(SDT)have attracted substantial attention for their efficacy in cancer treatment,and have been combined with chemotherapy to overcome drug resistance.However,simultaneously delivering sensitizers and chemotherapy drugs to same tumor cell remains challenging,thus greatly limiting this combinational therapy.The rapid development of nanotechnology provides a new approach to solve this problem.Nano-based drug delivery systems can not only improve the targeted delivery of agents but also co-deliver multiple drug components in single nanoparticles to achieve optimal synergistic effects.In this review,we briefly summarize the mechanisms of drug resistance,discuss the advantages and disadvantages of PDT and SDT in reversing drug resistance,and describe state-of-the-art research using nano-mediated PDT and SDT to solve these refractory problems.This review also highlights the clinical translational potential for this combinational therapy.
基金partially supported by the National Natural Science Foundation of China(81702457)the Clinical Medical University and Hospital Joint Construction of Disciplinary Projects 2021(2021lcxk017)+4 种基金the Guangdong Provincial Key Laboratory of Precision Medicine for Gastrointestinal Cancer(2020B121201004)the Outstanding Youths Development Scheme of Nanfang Hospital,Southern Medical University(2021J008)the Basic and Clinical Cooperative Research and Promotion Program of Anhui Medical University(2021xkjT028)the Open Fund of Key Laboratory of Antiinflammatory and Immune Medicine(KFJJ-2021-11)Grants for Scientific Research of BSKY from Anhui Medical University(1406012201)。
文摘Glioblastoma multiforme(GBM) is the most common primary malignant brain tumor, and it is associated with poor prognosis. Its characteristics of being highly invasive and undergoing heterogeneous genetic mutation, as well as the presence of the blood–brain barrier(BBB), have reduced the efficacy of GBM treatment. The emergence of a novel therapeutic method, namely, sonodynamic therapy(SDT), provides a promising strategy for eradicating tumors via activated sonosensitizers coupled with low-intensity ultrasound. SDT can provide tumor killing effects for deep-seated tumors, such as brain tumors. However, conventional sonosensitizers cannot effectively reach the tumor region and kill additional tumor cells, especially brain tumor cells. Efforts should be made to develop a method to help therapeutic agents pass through the BBB and accumulate in brain tumors. With the development of novel multifunctional nanosensitizers and newly emerging combination strategies, the killing ability and selectivity of SDT have greatly improved and are accompanied with fewer side effects. In this review, we systematically summarize the findings of previous studies on SDT for GBM, with a focus on recent developments and promising directions for future research.
基金the National Natural Science Foundation of China(NSFC,Nos.51720105015,51972138,51929201,51922097,51772124 and 51872282)the Science and Technology Cooperation Project between Chinese and Australian Governments(No.2017YFE0132300)the Key Research Program of Frontier Sciences,CAS(No.YZDY-SSW-JSC018)。
文摘Compared with traditional photodynamic therapy(PDT),ultrasound(US)triggered sonodynamic therapy(SDT)has a wide application prospect in tumor therapy because of its deeper penetration depth.Herein,a novel MnSiO_(3)-Pt(MP)nanocomposite composed of Mn Si O_(3)nanosphere and noble metallic Pt was successfully constructed.After modification with bovine serum albumin(BSA)and chlorine e6(Ce6),the multifunctional nanoplatform Mn SiO_(3)-Pt@BSA-Ce6(MPBC)realized the magnetic resonance imaging(MRI)-guided synergetic SDT/chemodynamic therapy(CDT).In this nanoplatform,sonosensitizer Ce6 can generate singlet oxygen(^(1)O_(2))to kill cancer cells under US irradiation.Meanwhile,the loaded Pt has the ability to catalyze the decomposition of overexpressed hydrogen peroxide(H_(2)O_(2))in tumor microenvironment(TME)to produce oxygen(O_(2)),which can conquer tumor hypoxia and promote the SDT-induced^(1)O_(2)production.In addition,MP can degrade in mildly acidic and reductive TME,causing the release of Mn^(2+).The released Mn^(2+) not only can be used for MRI,but also can generate hydroxyl radical(^·OH)for CDT by Fenton-like reaction.The multifunctional nanoplatform MPBC has high biological safety and good anticancer effect,which displays the great latent capacity in biological application.
基金support from the Natural Science Foundation of Henan,China(Grant No.:202300410446)the National Natural Science Foundation of China(Grant No.:82071950).
文摘Atherosclerosis(AS)is a chronic inflammatory disease of large and medium-sized arteries that leads to ischemic heart disease,stroke,and peripheral vascular disease.Despite the current treatments,mortality and disability still remain high.Sonodynamic therapy(SDT),a non-invasive and localized methodology,has been developed as a promising new treatment for inhibiting atherosclerotic progression and stabilizing plaques.Promising progress has been made through cell and animal assays,as well as clinical trials.For example,the effect of SDT on apoptosis and autophagy of cells in AS,especially macrophages,and the concept of non-lethal SDT has also been proposed.In this review,we summarize the ultrasonic parameters and known sonosensitizers utilized in SDT for AS;we elaborate on SDT's therapeutic effects and mechanisms in terms of macrophages,T lymphocytes,neovascularization,smooth muscle cells,lipid,extracellular matrix and efferocytosis within plaques;additionally,we discuss the safety of SDT.A comprehensive summary of the confirmed effects of SDT on AS is conducted to establish a framework for future researchers.
基金the National Natural Science Foundation of China,No.82272004 and No.81974470the Nature Science Foundation of Zhejiang Province,No.LZ22H180001.
文摘In recent years,because of the growing desire to improve the noninvasiveness and safety of tumor treatments,sonodynamic therapy has gradually become a popular research topic.However,due to the complexity of the therapeutic process,the relevant mechanisms have not yet been fully elucidated.One of the widely accepted possibilities involves the effect of reactive oxygen species.In this review,the mechanism of reactive oxygen species production by sonodynamic therapy(SDT)and ways to enhance the sonodynamic production of reactive oxygen species are reviewed.Then,the clinical application and limitations of SDT are discussed.In conclusion,current research on sonodynamic therapy should focus on the development of sonosensitizers that efficiently produce active oxygen,exhibit biological safety,and promote the clinical transformation of sonodynamic therapy.
文摘Deep-tissue solid cancer treatment has a poor prognosis,resulting in a very low 5-year patient survival rate.The primary challenges facing solid tumor therapies are accessibility,incomplete surgical removal of tumor tissue,the resistance of the hypoxic and heterogeneous tumor microenvironment to chemotherapy and radiation,and suffering caused by off-target toxicities.Here,sonodynamic therapy(SDT)is an evolving therapeutic approach that uses low-intensity ultrasound to target deep-tissue solid tumors.The ability of ultrasound to deliver energy safely and precisely into small deep-tissue(>10 cm)volumes makes SDT more effective than conventional photodynamic therapy.While SDT is currently in phase 1/2 clinical trials for glioblastoma multiforme,its use for other solid cancer treatments,such as breast,pancreatic,liver,and prostate cancer,is still in the preclinical stage,with further investigation required to improve its therapeutic efficacy.This review,therefore,focuses on recent advances in SDT cancer treatments.We describe the interaction between ultrasound and sonosensitizer molecules and the associated energy transfer mechanism to malignant cells,which plays a central role in SDT-mediated cell death.Different sensitizers used in clinical and preclinical trials of various cancer treatments are listed,and the critical ultrasound parameters for SDT are reviewed.We also discuss approaches to improve the efficacies of these sonosensitizers,the role of the 3-dimensional spheroid in vitro investigations,ultrasound-controlled CAR-T cell and SDT-based multimodal therapy,and machine learning for sonosensitizer optimization,which could facilitate clinical translation of SDT.
基金The project supported by the General Research Fund Grant from Hong Kong Research Grant Committee(476912)Health and Medical Research Fund(13120442)
文摘OBJECTIVE To investigate the effect of sonodynamic action on biofilm-producing Enterococcus faecalis using hypocrellin B from a natural product Hypocrella bambuase as a sensitizer.METHODS The uptake of hypocrellin B in biofilm-producing Enterococcus faecalis was measured using the fluorescent analysis method.After the combined treatment of hypocrellin B and ultrasound,the growth of biofilm-producing Enterococcus faecalis was measured using the colony counting method,the membrane integrity was detected using a flow cytometric analysis with propidium iodide staining.Chromosomal DNA of bacteria was analysed using apulsed-field gel electrophoresis(PFGE).RESULTS The uptake of hypocrellin B in biofilm-producing Enterocuccus faecalis reached a high peak at the 20 min incubation.After the combined treatment of hypocrellin B,the growth of biofilm-producing Enterocuccus faecalis significantly descreased and membrane integrity was remarkably damaged.However,no remarkable change in bacterial DNA was found.CONCLUSION Sonodynamic action of hypocrellin B had significant killing activity on Enterocuccus faecalis.
基金financially supported by the National Natural Science Foundation of China (Nos. 21873110 and 61720106014)the Instrument Developing Project of the Chinese Academy of Sciences (No. YJKYYQ20170015)
文摘Traditional cancer treatments have disadvantages of large trauma area and toxic side effects while killing cancer cells.Peptide-targeted sonodynamic therapy(SDT)can effectively improve specificity of cancer treatment and overcome the problem of low tissue penetration depth caused by a photo-driven therapy.Herein,we developed a porphyrin-based sonosensitizer with a water-soluble polymer as a biological carrier and a cRGD peptide for tumor targeting,which constituted a nano sonosensitizer(T-cRGD NPs)for fluorescence imaging-guided sonodynamic therapy.A comparable sonosensitizer(T-PEG NPs)without the targeting unit was also prepared for illustration of therapeutic performance.Attribute to the role of peptide targeting,T-cRGD NPs can accumulate and enter tumor cells for fluorescence imaging and show a superior SDT effect than T-PEG NPs in vitro.The imaging in vivo reveals that T-cRGD NPs can enrich in tumor tissues within 14 h with a good biocompatibility.
基金financial support of the National Natural Science Foundation of China (NSFC, Nos. 22077085, 22007104)the Project of the Natural Science Foundation of Guangdong Province(No. 2019A1515011958)+2 种基金the Science and Technology Foundation of Shenzhen (No. JCYJ20190808153209537)DST,the Government of India (No. DST/INSPIRE/04/2019/000492)the Instrumental Analysis Center of Shenzhen University。
文摘Metal-based compounds with excellent photo-physical properties show good photochemotherapeutic performance.But,low in-depth tissue penetration of light limits their effectivity for deeply buried tumors.Encouraged by the sonosensitizing ability of the traditional organic photosensitizers,here,we developed AuNPs@Ir1 as a sonosensitizer by hybridizing an organometallic Ir(Ⅲ) complex(Ir1) with ultrasmall gold nanoparticles(AuNPs) for efficient tumor sonodynamic therapy(SDT) for the first time.AuNPs@Ir1 rapidly entered the cancer cells,produced ^(1)O_(2),and catalytically oxidized NADH to NAD;under ultrasound(US)irradiation,thus resulted in cancer cells oncosis.Because of efficient passive retention in tumors post intravenous injection,AuNPs@Ir1 further efficiently inhibited the growth of tumors in-vivo under US stimulation without long-term toxicity to other organs.Overall,this work presents the excellent US triggered in-vitro and in-vivo anticancer profile of the novel AuNPs@Ir1.It is expected to increase the scope of SDT for metal-based anticancer drugs.
