Aim To study the effects of praenuptorin C(pra-C),a pure constituent isolated from"Qianhu",the roots of Peucedanum praenuptorum Dunn(Umbelliterae),on vascular hypertrophy,collagen content,transient[Ca^(2+)],...Aim To study the effects of praenuptorin C(pra-C),a pure constituent isolated from"Qianhu",the roots of Peucedanum praenuptorum Dunn(Umbelliterae),on vascular hypertrophy,collagen content,transient[Ca^(2+)],.NO and vascular response of the thoracic aorta of renovascular and spontaneously hypertensive rats(RHR,SHR),Methods RHR and SHR were given pra-C 20 mg*kg`'·d'for 9 weeks,ig.Blood pressurs of both rats were measured using tail cuff manom-etry.Under inverted microscopy the length and width of the smooth muscle cells were measured by using computer software MICC(Dongnan University).[Ca^(2+)],of smooth muscle cells(SMCs)was measured with Fuma-2/AM.By measuring the spe-cific amino acid hydroxyproline content,the collagen content was obtained.By using Griess reagent,the NO in the SMCs was measured.Results The intermedium of the thoracic aorta in RHR was larger than that of the normal and pra-C-treated groups.The area(length x width)of the SMCs of thoracic aorta from RHR was 73.4 pam us normal 34.5 pum and pra-C 34 pam.The collagen content of thonacic aorta was 39±6.8%dry weight in RHR.while 26.5±3%in nomal and 25.6±1.1%in pm-C-treated RHR.The resting[Ca^(2+)],of SMCs was(150±8),(226±11)and(362±18)nmol·L’'separately in normal rats,RHR and SHR.In the presence of KCl 60 mmol·L',NE 10 pmol·L^(-1),ANG I 100 nmol·L^(-1)and ATP 30 pmol·L^(-1),the[Ca^(2+)],of SMCs were increased by 128%;132%;233%and 152%in RHR.respectively.Similarly,60 mmol·L'KCl and 10 pumol·L^(-1)NE also increased the[Ca*],of SMCs by 235%and 200%in$HR.Pretreating with pra-C could decrease the level of[Ca'’],to the normal level whether in RHR or in SHR.The amount of NO of SMCs was de-creased 76%in SHR compared with nomnal group,while pra-C could recover this change.In addition,pra-Cimproved the vascular responses of thoracic aorta of RHR.Conclusion These results indicate that pra-C improved the vascular hypertrophy by decreasing the area of SMCs,collagen content,SMCs[Ca’],and increasing NO production.展开更多
Objective To determine the increase of apoptosis and the decrease of smooth muscle cells (SMCs) density in human abdominal aortic aneurysms (AAA). Methods In situ terminal transferase-mediated dUTP nick end labeling (...Objective To determine the increase of apoptosis and the decrease of smooth muscle cells (SMCs) density in human abdominal aortic aneurysms (AAA). Methods In situ terminal transferase-mediated dUTP nick end labeling (TUNEL) was employed to detect apoptosis of SMCs in patients with AAA (n=25) and normal abdominal aortae (n=10). Positive cells were identified by specific cell marker in combination with immunohistochemistry. Meanwhile SMC counting was performed by anti-α-actin immunohistostaining to compare the SMC density. Results TUNEL staining revealed that there was significantly increased apoptosis in AAAs (average 8.6%) compared with normal abdominal aortae (average 0.95%, P <0.01). Double staining showed that most of these cells were SMCs. Counting of α-actin positive SMCs revealed that medial SMC density of AAAs (37.5±7.6 SMCs /HPF) was reduced by 79.1% in comparison with that of normal abdominal aortae (179.2±16.1 SMCs /HPF,P <0.01). Conclusions Significantly increased SMCs of AAA bear apoptotic markers initiating cell death. Elevated apoptosis may result in a decreased density of SMCs in AAA,which may profoundly influence the development of AAA.展开更多
基金supported by grants from the National Natural Science Foundation of China(39570819)Fimt published in Chinese in Acta Phamaceutica Sinica.2001,36:165.
文摘Aim To study the effects of praenuptorin C(pra-C),a pure constituent isolated from"Qianhu",the roots of Peucedanum praenuptorum Dunn(Umbelliterae),on vascular hypertrophy,collagen content,transient[Ca^(2+)],.NO and vascular response of the thoracic aorta of renovascular and spontaneously hypertensive rats(RHR,SHR),Methods RHR and SHR were given pra-C 20 mg*kg`'·d'for 9 weeks,ig.Blood pressurs of both rats were measured using tail cuff manom-etry.Under inverted microscopy the length and width of the smooth muscle cells were measured by using computer software MICC(Dongnan University).[Ca^(2+)],of smooth muscle cells(SMCs)was measured with Fuma-2/AM.By measuring the spe-cific amino acid hydroxyproline content,the collagen content was obtained.By using Griess reagent,the NO in the SMCs was measured.Results The intermedium of the thoracic aorta in RHR was larger than that of the normal and pra-C-treated groups.The area(length x width)of the SMCs of thoracic aorta from RHR was 73.4 pam us normal 34.5 pum and pra-C 34 pam.The collagen content of thonacic aorta was 39±6.8%dry weight in RHR.while 26.5±3%in nomal and 25.6±1.1%in pm-C-treated RHR.The resting[Ca^(2+)],of SMCs was(150±8),(226±11)and(362±18)nmol·L’'separately in normal rats,RHR and SHR.In the presence of KCl 60 mmol·L',NE 10 pmol·L^(-1),ANG I 100 nmol·L^(-1)and ATP 30 pmol·L^(-1),the[Ca^(2+)],of SMCs were increased by 128%;132%;233%and 152%in RHR.respectively.Similarly,60 mmol·L'KCl and 10 pumol·L^(-1)NE also increased the[Ca*],of SMCs by 235%and 200%in$HR.Pretreating with pra-C could decrease the level of[Ca'’],to the normal level whether in RHR or in SHR.The amount of NO of SMCs was de-creased 76%in SHR compared with nomnal group,while pra-C could recover this change.In addition,pra-Cimproved the vascular responses of thoracic aorta of RHR.Conclusion These results indicate that pra-C improved the vascular hypertrophy by decreasing the area of SMCs,collagen content,SMCs[Ca’],and increasing NO production.
文摘Objective To determine the increase of apoptosis and the decrease of smooth muscle cells (SMCs) density in human abdominal aortic aneurysms (AAA). Methods In situ terminal transferase-mediated dUTP nick end labeling (TUNEL) was employed to detect apoptosis of SMCs in patients with AAA (n=25) and normal abdominal aortae (n=10). Positive cells were identified by specific cell marker in combination with immunohistochemistry. Meanwhile SMC counting was performed by anti-α-actin immunohistostaining to compare the SMC density. Results TUNEL staining revealed that there was significantly increased apoptosis in AAAs (average 8.6%) compared with normal abdominal aortae (average 0.95%, P <0.01). Double staining showed that most of these cells were SMCs. Counting of α-actin positive SMCs revealed that medial SMC density of AAAs (37.5±7.6 SMCs /HPF) was reduced by 79.1% in comparison with that of normal abdominal aortae (179.2±16.1 SMCs /HPF,P <0.01). Conclusions Significantly increased SMCs of AAA bear apoptotic markers initiating cell death. Elevated apoptosis may result in a decreased density of SMCs in AAA,which may profoundly influence the development of AAA.
