Objective:To give a brief overview of the field of epigenetics and the potential predictive power that small non-coding RNA(sncRNA)may hold in relation to improving the treatment and diagnosis of male infertility.Meth...Objective:To give a brief overview of the field of epigenetics and the potential predictive power that small non-coding RNA(sncRNA)may hold in relation to improving the treatment and diagnosis of male infertility.Methods:PRISMA-ScR was used as the scoping review guideline for this investigation.All article data here have been accessed from MEDLINE–PubMed,Science Direct,EBSCO,Scopus,Sage Journals,and Google Scholar.The terms"small non coding RNA,male,infertility,miRNA,sperm"were used in the search between 2015 and 2023.Results:The study comprised 35 publications in total.Several sncRNAs,miR-155,miR-16,miR-196,miR-525-3p,miR-891 were found to be effective in regulating the mechanism of spermatozoa processing in the infertility of men.sncRNA can be used as a biomarker of male infertility.Conclusions:sncRNAs can act as biomarkers for the diagnosis of reproductive diseases.Actually,by recognizing sncRNAs and their mechanisms,a new way to treat infertile men would be paved.The functional annotation of sncRNAs in spermatogenesis is still in its infancy but has enormous potential.This is despite the fact that many potential sncRNAs have been found to date with the use of cutting-edge technology and publicly accessible sncRNA annotation tools.展开更多
Endometrial cancer （EC） is the most common and lethal gynaecological cancer type in Europe and in North America. Frequently EC arises more in the corpus proper and manifests as round, polypoid expansile masses, but ...Endometrial cancer （EC） is the most common and lethal gynaecological cancer type in Europe and in North America. Frequently EC arises more in the corpus proper and manifests as round, polypoid expansile masses, but it may also originate in the lower uterine segment or spread in endometrium with necrosis and hemorrhage. The analysis was performed using a custom panel containing all DNA sequences loci coding pre-miRNAs and genes related to biogenesis and regulation of sncRNAs in normal and tumor tissues extracted from 6 unrelated patients with endometrial carcinoma. The identified variations were correlated with mature miRNAs differentially expressed in the same normal and tumor endometrial tissues. The comparison analysis confirmed the high degree of cellular and genetic intratumoral heterogeneity with a temporal and spatial miRNA expression distribution in association with genomic variants identified. The classification of specific DNA mutations, onto the loci identified, should be suitable to characterize possible instability genome regions and help classification of tumors to ameliorate the clinical management of patients affected by endometrial carcinoma.展开更多
目的利用PANDORA-seq鉴定年轻和老年小鼠心房组织中的sncRNA表达差异,观察姜黄素干预对老年小鼠差异sncRNA和靶基因的影响。方法年轻组(5个月龄)和老年组(18个月龄)小鼠心房组织进行PANDORA-seq,SPORTS1.1注释sncRNA的表达谱,R包DESeq2...目的利用PANDORA-seq鉴定年轻和老年小鼠心房组织中的sncRNA表达差异,观察姜黄素干预对老年小鼠差异sncRNA和靶基因的影响。方法年轻组(5个月龄)和老年组(18个月龄)小鼠心房组织进行PANDORA-seq,SPORTS1.1注释sncRNA的表达谱,R包DESeq2展示差异sncRNA,对差异miRNA进行靶基因预测和构建miRNA-mRNA网络,实时定量聚合酶链反应(quantitative real time polymerase chain reaction,qRT-PCR)鉴定靶分子。姜黄素干预后,在体电生理标测和Western-blot观察老年小鼠心房颤动可诱发率,心房衰老程度及衰老和纤维化相关蛋白的表达。结果从年轻组和老年组小鼠心房组织中鉴定出大量sncRNA,包括miRNA、tsRNA、rsRNA和piRNA,老年组sncRNA表达丰度比年轻组明显增多(P=0.048),rsRNA和tsRNA的起源位点也存在差异。两组小鼠间4种sncRNA存在大量序列差异,而其种类差异只存在于miRNA中,且老年组小鼠miR-298(P=0.005)和miR-301b(P=0.004)明显降低。姜黄素干预可使老年小鼠心房组织中miR-298(P=0.000)和miR-301b(P=0.000)表达上调,降低衰老和纤维化蛋白的表达和改善心房颤动易感性。结论sncRNA参与小鼠心房衰老过程,姜黄素可靶向miR-298和miR-301b参与心房衰老相关纤维化过程,为老年心房颤动的防治提供新思路。展开更多
With the advancement of high-throughput sequencing and bioinformatics,an increasing number of overlooked small noncoding RNAs(sncRNAs)have emerged.These sncRNAs predominantly comprise transfer RNA-derived fragments(ts...With the advancement of high-throughput sequencing and bioinformatics,an increasing number of overlooked small noncoding RNAs(sncRNAs)have emerged.These sncRNAs predominantly comprise transfer RNA-derived fragments(tsRNAs),PIWI-interacting RNAs(piRNAs),Ro-associated non-coding RNAs(RNYs or Y-RNAs),small nucleolar RNAs(snoR-NAs),and small nuclear RNAs(snRNAs).Each of these RNA types possesses distinct biological properties and plays specific roles in both physiological and pathological processes.The differ-ential expression of sncRNAs substantially affects the occurrence and progression of various systemic diseases.However,their roles in the cardiovascular system remain unclear.There-fore,understanding the functionality and mechanisms of sncRNAs in the cardiovascular system holds promise for identifying novel targets and strategies for the diagnosis,prevention,and treatment of cardiovascular diseases.This review examines the biological characteristics of sncRNAs and their potential roles in cardiovascular diseases.展开更多
文摘Objective:To give a brief overview of the field of epigenetics and the potential predictive power that small non-coding RNA(sncRNA)may hold in relation to improving the treatment and diagnosis of male infertility.Methods:PRISMA-ScR was used as the scoping review guideline for this investigation.All article data here have been accessed from MEDLINE–PubMed,Science Direct,EBSCO,Scopus,Sage Journals,and Google Scholar.The terms"small non coding RNA,male,infertility,miRNA,sperm"were used in the search between 2015 and 2023.Results:The study comprised 35 publications in total.Several sncRNAs,miR-155,miR-16,miR-196,miR-525-3p,miR-891 were found to be effective in regulating the mechanism of spermatozoa processing in the infertility of men.sncRNA can be used as a biomarker of male infertility.Conclusions:sncRNAs can act as biomarkers for the diagnosis of reproductive diseases.Actually,by recognizing sncRNAs and their mechanisms,a new way to treat infertile men would be paved.The functional annotation of sncRNAs in spermatogenesis is still in its infancy but has enormous potential.This is despite the fact that many potential sncRNAs have been found to date with the use of cutting-edge technology and publicly accessible sncRNA annotation tools.
文摘Endometrial cancer （EC） is the most common and lethal gynaecological cancer type in Europe and in North America. Frequently EC arises more in the corpus proper and manifests as round, polypoid expansile masses, but it may also originate in the lower uterine segment or spread in endometrium with necrosis and hemorrhage. The analysis was performed using a custom panel containing all DNA sequences loci coding pre-miRNAs and genes related to biogenesis and regulation of sncRNAs in normal and tumor tissues extracted from 6 unrelated patients with endometrial carcinoma. The identified variations were correlated with mature miRNAs differentially expressed in the same normal and tumor endometrial tissues. The comparison analysis confirmed the high degree of cellular and genetic intratumoral heterogeneity with a temporal and spatial miRNA expression distribution in association with genomic variants identified. The classification of specific DNA mutations, onto the loci identified, should be suitable to characterize possible instability genome regions and help classification of tumors to ameliorate the clinical management of patients affected by endometrial carcinoma.
文摘目的利用PANDORA-seq鉴定年轻和老年小鼠心房组织中的sncRNA表达差异,观察姜黄素干预对老年小鼠差异sncRNA和靶基因的影响。方法年轻组(5个月龄)和老年组(18个月龄)小鼠心房组织进行PANDORA-seq,SPORTS1.1注释sncRNA的表达谱,R包DESeq2展示差异sncRNA,对差异miRNA进行靶基因预测和构建miRNA-mRNA网络,实时定量聚合酶链反应(quantitative real time polymerase chain reaction,qRT-PCR)鉴定靶分子。姜黄素干预后,在体电生理标测和Western-blot观察老年小鼠心房颤动可诱发率,心房衰老程度及衰老和纤维化相关蛋白的表达。结果从年轻组和老年组小鼠心房组织中鉴定出大量sncRNA,包括miRNA、tsRNA、rsRNA和piRNA,老年组sncRNA表达丰度比年轻组明显增多(P=0.048),rsRNA和tsRNA的起源位点也存在差异。两组小鼠间4种sncRNA存在大量序列差异,而其种类差异只存在于miRNA中,且老年组小鼠miR-298(P=0.005)和miR-301b(P=0.004)明显降低。姜黄素干预可使老年小鼠心房组织中miR-298(P=0.000)和miR-301b(P=0.000)表达上调,降低衰老和纤维化蛋白的表达和改善心房颤动易感性。结论sncRNA参与小鼠心房衰老过程,姜黄素可靶向miR-298和miR-301b参与心房衰老相关纤维化过程,为老年心房颤动的防治提供新思路。
基金supported by the Zhanjiang Science and Technology Plan Project(No.2022A01143,2023E0005,2022A01149,2021A05094)Discipline Construction Project of Guangdong Medical University(No.GDMXK2021001)+1 种基金Research Project of Guangdong Provincial Bureau of Traditional Chinese Medicine(No.20232213)Postdoctoral Research Project of the Second Affiliated Hospital of Guangdong Medical University(No.22H01)(all China).
文摘With the advancement of high-throughput sequencing and bioinformatics,an increasing number of overlooked small noncoding RNAs(sncRNAs)have emerged.These sncRNAs predominantly comprise transfer RNA-derived fragments(tsRNAs),PIWI-interacting RNAs(piRNAs),Ro-associated non-coding RNAs(RNYs or Y-RNAs),small nucleolar RNAs(snoR-NAs),and small nuclear RNAs(snRNAs).Each of these RNA types possesses distinct biological properties and plays specific roles in both physiological and pathological processes.The differ-ential expression of sncRNAs substantially affects the occurrence and progression of various systemic diseases.However,their roles in the cardiovascular system remain unclear.There-fore,understanding the functionality and mechanisms of sncRNAs in the cardiovascular system holds promise for identifying novel targets and strategies for the diagnosis,prevention,and treatment of cardiovascular diseases.This review examines the biological characteristics of sncRNAs and their potential roles in cardiovascular diseases.
