Microbial communities in different gastrointestinal regions （stomach, foregut, midgut, and hindgut） of the northern snakehead Channa argus （Cantor, 1842） were compared by polymerase chain reaction and partial 16S ...Microbial communities in different gastrointestinal regions （stomach, foregut, midgut, and hindgut） of the northern snakehead Channa argus （Cantor, 1842） were compared by polymerase chain reaction and partial 16S rDNA sequencing. A total of 194, 140, 212, and 122 OTUs were detected in the stomach, foregut, midgut, and hindgut, respectively. Significant differences were found in the Sobs, ACE, Shannon, and Simpson indices among samples （P〈0.05）. The gastrointestinal microbial community of C. argus consisted predominantly of Proteobacteria with either Halomonas, Shewanella, Plesiomonas, or Sphingomonas. Fusobacteria, Firm!cutes, and Bacteroidetes also existed in the gastrointestinal tracts. However, significant＇differences were found in the compositions of microbial community among the four regions （P〈0.05）. Cyanobacteria and Spirochetes were significantly higher in the midgut and hindgut （P〈0.05）. Fusobacteria and Firmicutes were dominant in the hindgut and foregut, respectively （P〈0.05）. Proteobacteria was the lowest in the hindgut （P〈0.05）. At genus level, Cetobacterium and Plesiomonas were significantly higher in the hindgut than in the other three samples （P〈0.05）. Clostridium and Prevotella were the highest in the midgut （P〈0.05）. Halomonas, Shewanella, and Sphingomonas were the highest in the foregut （P〈0.05）. Paracoccus and Vibrio were the highest in the stomach. Several genera were only detected in certain regions, as follows： stomach, Paracoccus and Hbrio; foregut, Halomonas, Shewanella, and Sphingomonas; midgut, Clostridium and Prevotella; and hindgut, Cetobacterium and Plesiomonas （P〈0.05）. At the species level, Acinetobacter rhizosphaerae was only detected in the stomach. Prevotella copri and Clostridium perfring were not detected in the foregut and midgut, respectively, whereas Prevotella copri and Faecalibacterium pra were not detected in the hindgut. These findings provide valuable information on the microbial community in each gastrointestinal region of C. argus. Moreover, this study indicated that microbial community was not only related to rearing environment but also to the physico-chemical characteristics of each gastrointestinal region.展开更多
Northern snakehead(Channa argus)is an important freshwater fish species in China,with a recently discovered yellow-mutant(YM)strain.The YM snakehead holds significant potential for selective breeding and commercial va...Northern snakehead(Channa argus)is an important freshwater fish species in China,with a recently discovered yellow-mutant(YM)strain.The YM snakehead holds significant potential for selective breeding and commercial value due to its superior appearance.However,its muscle quality differences compared to the wild-type(WT)strain remain unclear.This study compared flesh quality traits between YM and WT snakeheads and applied integrated transcriptomic and metabolomic analyses to uncover underlying molecular mechanisms.The flesh quality measurement results revealed that YM snakehead exhibited significantly higher muscle redness and n-3 PUFA content,particularly DHA,along with a significantly lower fishy odor compared to WT.Metabolomic profiling identified 42 differential metabolites(DMs),including DHA,IMP,and glycerophosphocholine related to lipid metabolism,and nicotinamide,trigonelline,and glutathione involved in redox processes.Transcriptomic analysis revealed 380 differentially expressed genes(DEGs),enriched in pathways oxidative phosphorylation(e.g.,myh13,atp5f1e)and lipid metabolism(e.g.,pnpla7,lipe).Integrated omics analysis suggested that lipid metabolism related DEGs and DMs may modulate nutritional value and flavor via the“Glycerophospholipid metabolism”pathway,while redox related metabolites and genes may influence muscle color by modulating myoglobin accumulation via the“Nicotinate and nicotinamide metabolism”pathway.These findings not only provide evidence supporting the superior flesh quality of the YM strain over the wild type and elucidate the molecular mechanisms underlying flesh quality differences between the two strains,but also offer a theoretical foundation for evaluating the economic potential of YM snakehead and guiding future breeding programs in aquaculture.展开更多
基金Supported by the National Natural Science Foundation of China(No.31402306)
文摘Microbial communities in different gastrointestinal regions （stomach, foregut, midgut, and hindgut） of the northern snakehead Channa argus （Cantor, 1842） were compared by polymerase chain reaction and partial 16S rDNA sequencing. A total of 194, 140, 212, and 122 OTUs were detected in the stomach, foregut, midgut, and hindgut, respectively. Significant differences were found in the Sobs, ACE, Shannon, and Simpson indices among samples （P〈0.05）. The gastrointestinal microbial community of C. argus consisted predominantly of Proteobacteria with either Halomonas, Shewanella, Plesiomonas, or Sphingomonas. Fusobacteria, Firm!cutes, and Bacteroidetes also existed in the gastrointestinal tracts. However, significant＇differences were found in the compositions of microbial community among the four regions （P〈0.05）. Cyanobacteria and Spirochetes were significantly higher in the midgut and hindgut （P〈0.05）. Fusobacteria and Firmicutes were dominant in the hindgut and foregut, respectively （P〈0.05）. Proteobacteria was the lowest in the hindgut （P〈0.05）. At genus level, Cetobacterium and Plesiomonas were significantly higher in the hindgut than in the other three samples （P〈0.05）. Clostridium and Prevotella were the highest in the midgut （P〈0.05）. Halomonas, Shewanella, and Sphingomonas were the highest in the foregut （P〈0.05）. Paracoccus and Vibrio were the highest in the stomach. Several genera were only detected in certain regions, as follows： stomach, Paracoccus and Hbrio; foregut, Halomonas, Shewanella, and Sphingomonas; midgut, Clostridium and Prevotella; and hindgut, Cetobacterium and Plesiomonas （P〈0.05）. At the species level, Acinetobacter rhizosphaerae was only detected in the stomach. Prevotella copri and Clostridium perfring were not detected in the foregut and midgut, respectively, whereas Prevotella copri and Faecalibacterium pra were not detected in the hindgut. These findings provide valuable information on the microbial community in each gastrointestinal region of C. argus. Moreover, this study indicated that microbial community was not only related to rearing environment but also to the physico-chemical characteristics of each gastrointestinal region.
基金supported by Shandong Technical System of Fish In-dustry(SDAIT-12-03).
文摘Northern snakehead(Channa argus)is an important freshwater fish species in China,with a recently discovered yellow-mutant(YM)strain.The YM snakehead holds significant potential for selective breeding and commercial value due to its superior appearance.However,its muscle quality differences compared to the wild-type(WT)strain remain unclear.This study compared flesh quality traits between YM and WT snakeheads and applied integrated transcriptomic and metabolomic analyses to uncover underlying molecular mechanisms.The flesh quality measurement results revealed that YM snakehead exhibited significantly higher muscle redness and n-3 PUFA content,particularly DHA,along with a significantly lower fishy odor compared to WT.Metabolomic profiling identified 42 differential metabolites(DMs),including DHA,IMP,and glycerophosphocholine related to lipid metabolism,and nicotinamide,trigonelline,and glutathione involved in redox processes.Transcriptomic analysis revealed 380 differentially expressed genes(DEGs),enriched in pathways oxidative phosphorylation(e.g.,myh13,atp5f1e)and lipid metabolism(e.g.,pnpla7,lipe).Integrated omics analysis suggested that lipid metabolism related DEGs and DMs may modulate nutritional value and flavor via the“Glycerophospholipid metabolism”pathway,while redox related metabolites and genes may influence muscle color by modulating myoglobin accumulation via the“Nicotinate and nicotinamide metabolism”pathway.These findings not only provide evidence supporting the superior flesh quality of the YM strain over the wild type and elucidate the molecular mechanisms underlying flesh quality differences between the two strains,but also offer a theoretical foundation for evaluating the economic potential of YM snakehead and guiding future breeding programs in aquaculture.
