In this study, the total phenols （TP） and antioxidant activity of three different fruit smoothies （Blackberries, Raspberry and Boysenberry （BRAB）, Mango and Passion fruits （MAP） and Blackcurrant, Acerola cherri...In this study, the total phenols （TP） and antioxidant activity of three different fruit smoothies （Blackberries, Raspberry and Boysenberry （BRAB）, Mango and Passion fruits （MAP） and Blackcurrant, Acerola cherries and Rosehips （BACAR）） were determined during storage by Folin-Ciocalteu and ferric reducing antioxidant power （FRAP） assays respectively. TP ranged from 1,943 to 2,692 mg L-1 gallic acid equivalent whereas FRAP from 43,217 to 126,125 μmol L-1 Trolox equivalent for the three smoothies on opening. BACAR had the highest TP and antioxidant activity. The smoothies showed significant decrease （P 〈 0.05） in TP during storage. The decrease in antioxidant activity was significant （P 〈 0.05） for BRAB and MAP only. However, the study revealed that the smoothies had high antioxidant activity even during shelf-life at 4 ℃ which implies that consumption of smoothies may give considerable antioxidant benefits. Correlation between TP and antioxidant activity was strong and highly significant （r = 0.890, P 〈 0.0001）.展开更多
Fruits and vegetables are laden with antioxidants and have been used to quench reactive oxygen species.The combination of two or more fruits and vegetables to produce smoothies is expected to yield a synergistic effec...Fruits and vegetables are laden with antioxidants and have been used to quench reactive oxygen species.The combination of two or more fruits and vegetables to produce smoothies is expected to yield a synergistic effect that could further improve functionality.This study investigated the antioxidative and blood glucose-lowering potentials of smoothies made from selected fruits and vegetables as well as their effect on the expression of pro-inflammatory genes in streptozotocin-induced diabetic Wistar rats.Standard procedures were used for the in vitro antioxidant determination while pancreas tissue was excised for molecular study.The inclusion of beetroot increased the total phenolic contents by 30-50%while the flavonoid content was increased by more than 3-fold.Similarly,smoothies with beetroot and bitter gourd exhibited stronger free radical scavenging abilities as shown by DPPH(1,1-diphenyl-2-picrylhydrazyl),ABTS(2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic)acid),Nitric oxide(NO)scavenging ability,metal chelation(Fe^(2+))and ferric reducing antioxidant properties(FRAP)compared to the sample devoid of the duo.The blood glucose levels of the rats fed the smoothie were significantly lowered with a similar trend in the animals that were administered metformin.The smoothies significantly(p<0.05)reduced the malondialdehyde(MDA)content thus reducing lipid peroxidation in the animals.The endogenous antioxidant enzymes;catalase(CAT),superoxide dismutase(SOD)and glutathione peroxidase(GPX)in the pancreas were all significantly(p<0.05)increased when the animals were fed smoothies.The expression of pro-inflammatory genes,IL-1β,IL-6 and TNF-α,was down-regulated in the animals fed with smoothies.The findings showed that the smoothies made from the combination have the potential for managing type-2 diabetes mellitus.展开更多
文摘In this study, the total phenols （TP） and antioxidant activity of three different fruit smoothies （Blackberries, Raspberry and Boysenberry （BRAB）, Mango and Passion fruits （MAP） and Blackcurrant, Acerola cherries and Rosehips （BACAR）） were determined during storage by Folin-Ciocalteu and ferric reducing antioxidant power （FRAP） assays respectively. TP ranged from 1,943 to 2,692 mg L-1 gallic acid equivalent whereas FRAP from 43,217 to 126,125 μmol L-1 Trolox equivalent for the three smoothies on opening. BACAR had the highest TP and antioxidant activity. The smoothies showed significant decrease （P 〈 0.05） in TP during storage. The decrease in antioxidant activity was significant （P 〈 0.05） for BRAB and MAP only. However, the study revealed that the smoothies had high antioxidant activity even during shelf-life at 4 ℃ which implies that consumption of smoothies may give considerable antioxidant benefits. Correlation between TP and antioxidant activity was strong and highly significant （r = 0.890, P 〈 0.0001）.
文摘Fruits and vegetables are laden with antioxidants and have been used to quench reactive oxygen species.The combination of two or more fruits and vegetables to produce smoothies is expected to yield a synergistic effect that could further improve functionality.This study investigated the antioxidative and blood glucose-lowering potentials of smoothies made from selected fruits and vegetables as well as their effect on the expression of pro-inflammatory genes in streptozotocin-induced diabetic Wistar rats.Standard procedures were used for the in vitro antioxidant determination while pancreas tissue was excised for molecular study.The inclusion of beetroot increased the total phenolic contents by 30-50%while the flavonoid content was increased by more than 3-fold.Similarly,smoothies with beetroot and bitter gourd exhibited stronger free radical scavenging abilities as shown by DPPH(1,1-diphenyl-2-picrylhydrazyl),ABTS(2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic)acid),Nitric oxide(NO)scavenging ability,metal chelation(Fe^(2+))and ferric reducing antioxidant properties(FRAP)compared to the sample devoid of the duo.The blood glucose levels of the rats fed the smoothie were significantly lowered with a similar trend in the animals that were administered metformin.The smoothies significantly(p<0.05)reduced the malondialdehyde(MDA)content thus reducing lipid peroxidation in the animals.The endogenous antioxidant enzymes;catalase(CAT),superoxide dismutase(SOD)and glutathione peroxidase(GPX)in the pancreas were all significantly(p<0.05)increased when the animals were fed smoothies.The expression of pro-inflammatory genes,IL-1β,IL-6 and TNF-α,was down-regulated in the animals fed with smoothies.The findings showed that the smoothies made from the combination have the potential for managing type-2 diabetes mellitus.
