A new cell line, CSEC, has been successfully established from embryos at gastrula stage of a cultured marine fish, half smooth tongue sole （Cynoglossus sernilaevis）. CSEC cells grow actively and stably more than 50 ...A new cell line, CSEC, has been successfully established from embryos at gastrula stage of a cultured marine fish, half smooth tongue sole （Cynoglossus sernilaevis）. CSEC cells grow actively and stably more than 50 passages for over 200 d in DMEM medium supplemented with 15% FBS （fetal bovine serum）, 2.5 ng/cm^3 bFGF （basic fibroblast growth factor）, 1 ng/cm^3 LIF （leukemia inhibitory factor） and 50 mmol/dm^3 2-ME （2-mecaptoethanol）. The cells grew well in the temperature range of 24-30 ℃ and the optimal growth temperature was 24 ℃. FBS and bFGF concentrations are the two key components for CSEC cells proliferation. Chromosome analysis reveals that CSEC cells have a normal diploid karyotype with 2n=42t. The significant fluorescent signals were observed in CSEC after transfection with the GFP reporter gene, suggesting that the CSEC cell line can be used as a useful tool for transgenic and genetic manipulation studies. CSEC cells showed the cytopathic effect （CPE） after infection with lymphosystis disease virus （LCDV） in 2 d. Moreover, the LCDV particles can be observed in the cytoplasm of virus-infected cells by electron microscopy. It suggests that CSEC could be potentially used for the study of aquatic virus.展开更多
In this paper, we present a nonmonotone smoothing Newton algorithm for solving the circular cone programming(CCP) problem in which a linear function is minimized or maximized over the intersection of an affine space w...In this paper, we present a nonmonotone smoothing Newton algorithm for solving the circular cone programming(CCP) problem in which a linear function is minimized or maximized over the intersection of an affine space with the circular cone. Based on the relationship between the circular cone and the second-order cone(SOC), we reformulate the CCP problem as the second-order cone problem(SOCP). By extending the nonmonotone line search for unconstrained optimization to the CCP, a nonmonotone smoothing Newton method is proposed for solving the CCP. Under suitable assumptions, the proposed algorithm is shown to be globally and locally quadratically convergent. Some preliminary numerical results indicate the effectiveness of the proposed algorithm for solving the CCP.展开更多
The goal of this paper is to investigate the bifurcation properties of stationary points of a class of planar piecewise smooth systems with 3 parameters using the theory of differential inclusions. We especially study...The goal of this paper is to investigate the bifurcation properties of stationary points of a class of planar piecewise smooth systems with 3 parameters using the theory of differential inclusions. We especially study the existence of the stationary points on the line of discontinuity of this kind of planar piecewise smooth system.展开更多
This paper is concerned with the bifurcation properties on the line of discontinuity of planar piecewise smooth systems. The existence of equilibria and periodic solutions with sliding motion in a class of planar piec...This paper is concerned with the bifurcation properties on the line of discontinuity of planar piecewise smooth systems. The existence of equilibria and periodic solutions with sliding motion in a class of planar piecewise smooth systems with 3-parameters is investigated in this paper using the theory of differential inclu-sion and tools of Poincar′e maps.展开更多
We give a characterization of the boundaries of smooth strictly convex sets in the Euclidean plane R2?based on the existence and uniqueness of inscribed triangles.
基金The State "863" High-Technology Research and Development Project of China under contract Nos 2006AA09Z406 and 2006AA10A401the National Natural Science Foundation of China under contract No. 40376047the Taishan Scholar Project of Shandong Province and MOA for returned scientists from abroad
文摘A new cell line, CSEC, has been successfully established from embryos at gastrula stage of a cultured marine fish, half smooth tongue sole （Cynoglossus sernilaevis）. CSEC cells grow actively and stably more than 50 passages for over 200 d in DMEM medium supplemented with 15% FBS （fetal bovine serum）, 2.5 ng/cm^3 bFGF （basic fibroblast growth factor）, 1 ng/cm^3 LIF （leukemia inhibitory factor） and 50 mmol/dm^3 2-ME （2-mecaptoethanol）. The cells grew well in the temperature range of 24-30 ℃ and the optimal growth temperature was 24 ℃. FBS and bFGF concentrations are the two key components for CSEC cells proliferation. Chromosome analysis reveals that CSEC cells have a normal diploid karyotype with 2n=42t. The significant fluorescent signals were observed in CSEC after transfection with the GFP reporter gene, suggesting that the CSEC cell line can be used as a useful tool for transgenic and genetic manipulation studies. CSEC cells showed the cytopathic effect （CPE） after infection with lymphosystis disease virus （LCDV） in 2 d. Moreover, the LCDV particles can be observed in the cytoplasm of virus-infected cells by electron microscopy. It suggests that CSEC could be potentially used for the study of aquatic virus.
基金supported by the National Natural Science Foundation of China(11401126,71471140 and 11361018)Guangxi Natural Science Foundation(2016GXNSFBA380102 and 2014GXNSFFA118001)+2 种基金Guangxi Key Laboratory of Cryptography and Information Security(GCIS201618)Guangxi Key Laboratory of Automatic Detecting Technology and Instruments(YQ15112 and YQ16112)China
文摘In this paper, we present a nonmonotone smoothing Newton algorithm for solving the circular cone programming(CCP) problem in which a linear function is minimized or maximized over the intersection of an affine space with the circular cone. Based on the relationship between the circular cone and the second-order cone(SOC), we reformulate the CCP problem as the second-order cone problem(SOCP). By extending the nonmonotone line search for unconstrained optimization to the CCP, a nonmonotone smoothing Newton method is proposed for solving the CCP. Under suitable assumptions, the proposed algorithm is shown to be globally and locally quadratically convergent. Some preliminary numerical results indicate the effectiveness of the proposed algorithm for solving the CCP.
基金The NSF (10671082) of Chinathe postgraduate program of 985 (20080239) of Jilin University
文摘The goal of this paper is to investigate the bifurcation properties of stationary points of a class of planar piecewise smooth systems with 3 parameters using the theory of differential inclusions. We especially study the existence of the stationary points on the line of discontinuity of this kind of planar piecewise smooth system.
文摘This paper is concerned with the bifurcation properties on the line of discontinuity of planar piecewise smooth systems. The existence of equilibria and periodic solutions with sliding motion in a class of planar piecewise smooth systems with 3-parameters is investigated in this paper using the theory of differential inclu-sion and tools of Poincar′e maps.
文摘We give a characterization of the boundaries of smooth strictly convex sets in the Euclidean plane R2?based on the existence and uniqueness of inscribed triangles.
