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Effects of interplay between metal subwavelength slits on extraordinary optical transmission 被引量:1
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作者 魏菲菲 王怀玉 周云松 《Chinese Physics B》 SCIE EI CAS CSCD 2013年第2期239-246,共8页
In this paper we study the extraordinary optical transmission of one-dimensional multi-slits in an ideal metal film.The transmissivity is calculated as a function of various structural parameters.The transmissivity os... In this paper we study the extraordinary optical transmission of one-dimensional multi-slits in an ideal metal film.The transmissivity is calculated as a function of various structural parameters.The transmissivity oscillates,with the period being just the light wavelength,as a function of the spacing between slits.As the number of slits increases,the transmissivity varies in one of three ways.It can increase,attenuate,or remain basically unchanged,depending on the spacing between slits.Each way is in an oscillatory manner.The slit interaction responsible for the oscillating transmission strength that depends on slit spacing is the subject of more detailed investigation.The interaction most intuitively manifests as a current distribution in the metal surface between slits.We find that this current is attenuated in an oscillating fashion from the slit corners to the center of the region between two adjacent slits,and we present a mathematical expression for its waveform. 展开更多
关键词 extraordinary optical transmission metal subwavelength slits interplay between slits
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Waveguide resonance of subwavelength metallic slits 被引量:1
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作者 陈跃刚 王艳花 +1 位作者 张岩 刘树田 《Chinese Physics B》 SCIE EI CAS CSCD 2007年第5期1315-1319,共5页
The transmission characteristics of a metallic film with subwavelength periodic slits are investigated by using the two-dimensional finite-difference time-domain method (2D-FDTD). Two models are constructed to show ... The transmission characteristics of a metallic film with subwavelength periodic slits are investigated by using the two-dimensional finite-difference time-domain method (2D-FDTD). Two models are constructed to show the dependance of the transmission spectrum on the slit structure. A sandwiched structure is used to exhibit the contribution of the metallic wall inside slits to the extraordinary high transmission. And a filled slit structure is employed to reflect the relation between the average refractive index inside the slits and the transmission spectrum of the structure. The transmission characteristics of two structures can be explained well with the waveguide resonance theory. 展开更多
关键词 electromagnetic wave propagation metallic subwavelength periodic slits waveguide resonance
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Ultra-elongated depth of focus of plasmonic lenses with concentric elliptical slits under Gaussian beam illumination
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作者 王婧 付永启 《Chinese Physics B》 SCIE EI CAS CSCD 2013年第9期209-213,共5页
In this paper, we discuss the influence of ratio of minor to major axis on the propagation property and focusing performance of a plasmonic lens with variant periodic concentric elliptical slits illuminating under a G... In this paper, we discuss the influence of ratio of minor to major axis on the propagation property and focusing performance of a plasmonic lens with variant periodic concentric elliptical slits illuminating under a Gaussian beam. In order to analyse the influence theoretically, a finite-difference time-domain (FDTD) numerical algorithm is adopted for the computational numerical calculation and the design of the plasmonic structure. The structure is flanked with penetrated slits through a 200-nm metal film (Au) which is coated on a quartz substrate. Tunability of focusing capability of the plasmonic lenses is studied by tailoring the ratio. Our calculation results demonstrate that the ratio of the elliptical slits greatly affects the focusing capability of the lense. The plasmonic lenses with concentric elliptical slits illuminating under a Gaussian beam have ultra-elongated depth of focus. These results are very encouraging for the future study of the plasmonic lens-based applications. 展开更多
关键词 ultra-elongated depth of focus plasmonic lenses elliptical slits finite-difference time-domain
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Subwavelength beam manipulation via multiple-metal slits coupled by disk-shaped nanocavity
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作者 郑改革 徐林华 +1 位作者 裴世鑫 陈云云 《Chinese Physics B》 SCIE EI CAS CSCD 2014年第3期320-325,共6页
A novel plasmonic structure consisting of three nano-scaled slits coupled by nano-disk-shaped nanocavities is pro- posed to produce subwavelength focusing and beam bending at optical frequencies. The incident light pa... A novel plasmonic structure consisting of three nano-scaled slits coupled by nano-disk-shaped nanocavities is pro- posed to produce subwavelength focusing and beam bending at optical frequencies. The incident light passes through the metal slits in the form of surface plasmon polaritons (SPPs) ,and then scatters into radiation fields. Numerical simulations using finite-difference time-domain (FDTD) method show that the transmitted fields through the design example can gener- ate light focusing and deflection by altering the refractive index of the coupled nanocavity. The simulation results indicate that the focal spot is beyond the diffraction limit. Light impinges on the surface at an angle to the optical axis will add an extra planar phase front that interferes with the asymmetric phase front of the plasmonic lens, leading to a larger bending angle off the axial direction. The advantages of the proposed plasmonic lens are smaller device size and ease of fabrication. Such geometries offer the potential to be controlled by using nano-positior!i0g systems for applications in dynamic beam shaping and scanning on the nanoscale. 展开更多
关键词 beam manipulation surface plasmon subwavelength slits
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THE INFLUENCE OF SELECTING RECEIVING SLITS AND USING RANDOMLY ORIENTED STANDARD SAMPLES ON QUANTITATIVE TEXTURE ANALYSIS
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作者 刘毓舒 《Journal of Shanghai Jiaotong university(Science)》 EI 2001年第1期31-35,共5页
The influence of the selection of receiving slits and the use of standard samples with random orientation on the result of quantitative texture analysis was tested and discussed. The result proves that it will improve... The influence of the selection of receiving slits and the use of standard samples with random orientation on the result of quantitative texture analysis was tested and discussed. The result proves that it will improve the precision of the analysis to use proper slits and a randomly oriented standard sample. A simple method was given to interpolate the correction curves of random intensities. 展开更多
关键词 quantitative texture analyses receiving slits randomly oriented standard samples
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Analysis of imbibition of n-alkanes in kerogen slits by molecular dynamics simulation for characterization of shale oil rocks 被引量:4
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作者 Qian Sang Xin-Yi Zhao +1 位作者 Hui-Min Liu Ming-Zhe Dong 《Petroleum Science》 SCIE CAS CSCD 2022年第3期1236-1249,共14页
Shale oil formations contain both inorganic and organic media.The organic matter holds both free oil in the pores and dissolved oil within the kerogen molecules.The free oil flow in organic pores and the dissolved oil... Shale oil formations contain both inorganic and organic media.The organic matter holds both free oil in the pores and dissolved oil within the kerogen molecules.The free oil flow in organic pores and the dissolved oil diffusion in kerogen molecules are coupled together.The molecular flow of free n-alkanes is an important process of shale oil accumulation and production.To study the dynamics of imbibition process of n-alkane molecules into kerogen slits,molecular dynamics(MD)simulations are conducted.Effects of slit width,temperature,and n-alkane types on the penetration speed,dynamic contact angle,and molecular conformations were analyzed.Results showed that molecular transportation of n-alkanes is dominated by molecular structure and molecular motion at this scale.The space-confinement conformational changes of molecules slow down the filling speeds in the narrow slits.The n-alkane molecules with long carbon chains require more time to undergo conformational changes.The high content of short-chain alkanes and high temperature facilitate the flow of alkane mixtures in kerogen slits.Results obtained from this study are useful for understanding the underlying nanoscale flow mechanism in shale formations. 展开更多
关键词 Molecular flow IMBIBITION N-ALKANE Kerogen slit Molecular dynamics simulation
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Analytical and experimental study on mild steel dampers with non-uniform vertical slits 被引量:2
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作者 Zheng Jie Li Aiqun Guo Tong 《Earthquake Engineering and Engineering Vibration》 SCIE EI CSCD 2015年第1期111-123,共13页
This study proposes a novel mild steel damper with non-uniform vertical slits. The influence of different shapes of vertical slits of the core energy plate on the energy dissipation and buckling resistance capacities ... This study proposes a novel mild steel damper with non-uniform vertical slits. The influence of different shapes of vertical slits of the core energy plate on the energy dissipation and buckling resistance capacities is analyzed. Based on the theoretical analysis, formulas of key parameters of the dampers, including the elastic lateral stiffness, shear bearing capacity and yield displacement, are derived. The effectiveness of the proposed damper is demonstrated through pseudo static tests on four 0.25-scale specimens. Performance of these dampers, i.e. cyclic deformation, stress distribution, energy dissipation capacity, etc., are presented and discussed. Using the numerical models of dampers calibrated through test data, earthquake time-history analyses were conducted, and it is observed that the dampers significantly reduce the seismic responses of the prototype frame and have a desirable energy dissipation capacity. 展开更多
关键词 mild steel damper vertical slit pseudo static test energy dissipation
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Flow characteristics at low Reynolds number around two in-line circular cylinders with slits 被引量:1
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作者 SUN Zhi-qiang ZHANG Yu-zhuo +1 位作者 XING Peng-fei LI Sai-wei 《Journal of Central South University》 SCIE EI CAS CSCD 2019年第8期2021-2028,共8页
This study investigated numerically the characteristics of laminar flow around two identical circular cylinders placed in tandem, with slits of the same width through their respective axis. The center to center distan... This study investigated numerically the characteristics of laminar flow around two identical circular cylinders placed in tandem, with slits of the same width through their respective axis. The center to center distance between the cylinders and the slit orientation were varied to study their effects on the flow structure, lift and drag, and vortex shedding characteristics. It was found that three flow regimes could be distinguished, the transitions between which could be indicated by the sudden changes in drag and lift. Asymmetrically, configured slits destabilized the stagnant region between cylinders;whereas in-line slits connect the two cylinders to act as a single elongated bluff body, even at large cylinder separation, by stabilizing the stagnant region in between. These in turn strongly modified the transition between flow regimes. Vortex shedding was also strongly influenced by both slit configuration and cylinder separation. 展开更多
关键词 two cylinders in tandem circular cylinder with slit vortex shedding laminar wake flow
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Dynamics of vortex–antivortex pair in a superconducting thin strip with narrow slits
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作者 何安 薛存 周又和 《Chinese Physics B》 SCIE EI CAS CSCD 2017年第4期449-456,共8页
In the framework of phenomenological time-dependent Ginzburg-Landau(TDGL) formalism,the dynamical properties of vortex-antivortex(V-Av) pair in a superconductor film with a narrow slit was studied.The slit positio... In the framework of phenomenological time-dependent Ginzburg-Landau(TDGL) formalism,the dynamical properties of vortex-antivortex(V-Av) pair in a superconductor film with a narrow slit was studied.The slit position and length can have a great impact not only on the vortex dynamical behavior but also the current-voltage(Ⅰ-Ⅴ) characteristics of the sample.Kinematic vortex lines can be predominated by the location of the slit.In the range of relatively low applied currents for a constant weak magnetic field,kinematic vortex line appears at right or left side of the slit by turns periodically.We found such single-side kinematic vortex line cannot lead to a jump in the Ⅰ-Ⅴ curve.At higher applied currents the phase-slip lines can be observed at left and right sides of the slit simultaneously.The competition between the vortex created at the lateral edge of the sample and the V-Av pair in the slit will result in three distinctly different scenarios of vortex dynamics depending on slit length:the lateral vortex penetrates the sample to annihilate the antivortex in the slit;the V-Av pair in the slit are driven off and expelled laterally;both the lateral vortex and the slit antivortex are depinned and driven together to annihilation in the halfway. 展开更多
关键词 vortex-antivortex pair phase-slip line current-voltage characteristic narrow slit
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轴突导向因子在α-突触核蛋白相关神经退行性疾病中的作用及机制
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作者 张瑛碧 李明徽 +2 位作者 张晓瑞 殷继红 王鹏 《中国组织工程研究》 北大核心 2026年第11期2834-2845,共12页
背景:轴突导向因子可能在α-突触核蛋白相关神经退行性疾病中发挥重要作用。目的:探讨轴突导向因子在α-突触核蛋白相关神经退行性疾病中的作用及机制。方法:由第一作者系统检索中国知网、万方数据库、PubMed、Nature、Embase、Web of S... 背景:轴突导向因子可能在α-突触核蛋白相关神经退行性疾病中发挥重要作用。目的:探讨轴突导向因子在α-突触核蛋白相关神经退行性疾病中的作用及机制。方法:由第一作者系统检索中国知网、万方数据库、PubMed、Nature、Embase、Web of Sciense、JAMA、BMJ等数据库,中文检索词为“帕金森病,轴突导向因子,路易体痴呆,多系统衰竭,单纯性自主神经功能衰竭”,英文检索词为“Parkinson’s disease,axon guidance molecules,Netrin,Ephrin,Semaphorin,Slit,Dementia with Lewy Bodies,Multiple System Atrophy,Pure Autonomic Failure”,全面收集并整理近年来有关轴突导向因子与α-突触核蛋白相关神经退行性疾病的文献,最终纳入89篇文献进行综述分析。结果与结论:轴突导向因子蛋白家族包括Netrins、Ephrins、Semaphorins和Slits。Netrins通过影响肠道因子、依赖DCC受体调节多巴胺能神经元活力,进而影响神经退行性疾病进程。在Ephrins家族中,EphrinA/EphA参与介导神经元再生信号,EphA1借CXC趋化因子配体12/CXC趋化因子受体4通路调节炎症与神经病理,影响疾病发展。Semaphorins家族中的Semaphorins-3通过引导多巴胺能神经元轴突从黑质向纹状体生长,进而改善神经退行性疾病的行为学症状。在多巴胺能神经元轴突传导通路上,Slits经Slit/Robo等信号协作调控轴突定位与寻路过程,确保神经元信号传导路径的准确性,对神经元连接的维持与修复具有重要意义。 展开更多
关键词 Α-突触核蛋白 轴突导向因子 帕金森病 多巴胺能神经元 Netrins EPHRINS SEMAPHORINS slits
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Slit3/Robo1通路探讨健骨颗粒对大鼠骨量丢失模型的影响
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作者 陈赛楠 黄云梅 +3 位作者 周芬 王志强 魏振朴 林燕萍 《中国骨质疏松杂志》 北大核心 2025年第3期313-320,共8页
目的观察健骨颗粒对去卵巢快速骨量丢失模型大鼠H型血管和Slit3/Robo1通路的影响,探讨其补虚化瘀的潜在作用机制。方法采用双侧卵巢切除术制备SD大鼠模型,灌胃干预6周,micro-CT观察胫骨近端微观结构,免疫荧光染色观察H型血管,ELISA检测... 目的观察健骨颗粒对去卵巢快速骨量丢失模型大鼠H型血管和Slit3/Robo1通路的影响,探讨其补虚化瘀的潜在作用机制。方法采用双侧卵巢切除术制备SD大鼠模型,灌胃干预6周,micro-CT观察胫骨近端微观结构,免疫荧光染色观察H型血管,ELISA检测血清CTX和BALP,Real-time PCR检测股骨Slit3和Robo1 mRNA的表达水平,免疫组织化学染色观察Slit3蛋白的表达水平,Western Blot检测Robo1蛋白的表达水平。结果模型组骨密度、骨小梁体积分数、骨小梁数量和骨小梁厚度均显著降低(P<0.01)和骨小梁分离度显著增大(P<0.001),H型血管阳性面积占比显著降低(P<0.001),Slit3 mRNA和蛋白表达水平显著升高(P<0.01),Robo1 mRNA和蛋白表达水平未见显著变化。与模型组相比,健骨颗粒组骨密度升高(P<0.05)和骨小梁分离度降低(P<0.001),H型血管阳性面积占比升高(P<0.05),血清CTX水平显著降低(P<0.001)、BALP水平略降低,Slit3 mRNA表达略下调和蛋白平均光密度值显著下降(P<0.05),Robo1 mRNA和蛋白表达水平显著升高(P<0.01)。结论健骨颗粒可通过Slit3/Robo1信号通路促进H型血管新生,提高骨量和改善骨微结构发挥抗骨质疏松的作用。 展开更多
关键词 绝经后骨质疏松症 健骨颗粒 H型血管 Slit3/Robo1信号通路
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狭缝引导配体1-3’非翻译区抑制心肌成纤维细胞纤维化表型的作用
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作者 王娅 伍华燕 +5 位作者 高原 吴茹诗 关佩莹 李晖 方俊涛 单志新 《中山大学学报(医学科学版)》 北大核心 2025年第3期466-474,共9页
【目的】研究狭缝引导配体1(Slit1)的3’非翻译区序列部分片段(Slit1-3’UTR)对心肌成纤维细胞的纤维化表型的调节作用和可能机制。【方法】利用腺病毒介导在ICR乳小鼠心肌成纤维细胞(mCFs)中过表达Slit1-3’UTR 1526nt序列(Slit1-3’UT... 【目的】研究狭缝引导配体1(Slit1)的3’非翻译区序列部分片段(Slit1-3’UTR)对心肌成纤维细胞的纤维化表型的调节作用和可能机制。【方法】利用腺病毒介导在ICR乳小鼠心肌成纤维细胞(mCFs)中过表达Slit1-3’UTR 1526nt序列(Slit1-3’UTR 1526nt),使用蛋白质免疫印迹技术评估mCFs中胶原Iα1/(COL1A1)、胶原IIIα1/(COL3A1)、α平滑肌肌动蛋白(α-SMA)等纤维化相关基因的表达水平,结合EdU和Trans-well实验评估对mCFs增殖和迁移能力的影响。利用血管紧张素II(AngⅡ)处理mCFs,检测Slit1-3’UTR 1526nt对于AngⅡ处理mCFs中纤维化表型的影响。过表达Slit1-3’UTR 1526nt后转染miR-34a-5p的类似物,放线菌素D干预实验检测Slit1-3’UTR 1526nt的mRNA的稳定性。过表达Slit1-3’UTR 1526nt检测mCFs中miR-34a-5p及其靶基因SIRT1的水平。分别转染miR-34a-5p和靶向SIRT1基因的小干扰RNA(si-SIRT1),检测对Slit1-3’UTR 1526nt调控mCFs纤维化表型的影响。【结果】利用腺病毒可在mCFs中有效介导过表达Slit1-3’UTR 1526nt,过表达Slit1-3’UTR1526nt可显著抑制mCFs纤维化基因的表达和mCFs增殖、迁移能力,抑制AngⅡ诱导mCFs的纤维化表型。放线菌素D实验结果显示转染miR-34a-5p降低mCFs中Slit1-3’UTR1526nt的稳定性,而过表达Slit1-3’UTR 1526nt时mCFs中miR-34a-5p水平降低。转染miR-34a-5p可促纤维化表型,并可逆转Slit1-3’UTR 1526nt对mCFs纤维化表型的抑制作用。在mCFs中过表达Slit1-3’UTR 1526nt显著升高miR-34a-5p靶基因SIRT1水平,在mCFs中分别转染miR-34a-5p和siRNA可一致性地逆转Slit1-3’UTR 1526nt抑制mCFs的纤维化表型。【结论】Slit1-3’UTR1526nt通过特异结合miR-34a-5p并增加其靶基因SIRT1表达发挥抑制mCFs纤维化表型的作用。 展开更多
关键词 心肌纤维化 狭缝引导配体1(Slit1) 3’非翻译区(3’UTR) 微RNA 心肌成纤维细胞
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Probing the fractional topological charge of a vortex light beam by using dynamic angular double slits 被引量:9
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作者 Jing Zhu Pei Zhang +5 位作者 Dongzhi Fu Dongxu Chen Ruifeng Liu Yingnan Zhou Hong Gao Fuli Li 《Photonics Research》 SCIE EI 2016年第5期187-190,共4页
Vortex beams with fractional topological charge(FTC) have many special characteristics and novel applications.However, one of the obstacles for their application is the difficulty of precisely determining the FTC of f... Vortex beams with fractional topological charge(FTC) have many special characteristics and novel applications.However, one of the obstacles for their application is the difficulty of precisely determining the FTC of fractional vortex beams. We find that when a vortex beam with an FTC illuminates a dynamic angular double slit(ADS), the far-field interference patterns that include the information of the FTC of the beam at the angular bisector direction of the ADS vary periodically. Based on this property, a simple dynamic ADS device and data fitting method can be used to precisely measure the FTC of a vortex light beam with an error of less than 5%. 展开更多
关键词 Probing the fractional topological charge of a vortex light beam by using dynamic angular double slits
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微小RNA-218靶向Slit导向配体2抑制四氯化碳诱导的小鼠肝纤维化的机制
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作者 张军鹏 王珏磊 +2 位作者 王超 舒瑾 潘鑫 《中华实验外科杂志》 2025年第10期1937-1941,共5页
目的探讨微小RNA-218(miRNA-218)通过靶向Slit导向配体2(SLIT2)对四氯化碳(CCl_(4))诱导的小鼠肝硬化的抑制作用及其分子机制。方法2023年3月至2024年12月,将60只C57BL/6雄性小鼠采用随机数字表法分为对照组、模型组(CCl_(4)诱导)及miRN... 目的探讨微小RNA-218(miRNA-218)通过靶向Slit导向配体2(SLIT2)对四氯化碳(CCl_(4))诱导的小鼠肝硬化的抑制作用及其分子机制。方法2023年3月至2024年12月,将60只C57BL/6雄性小鼠采用随机数字表法分为对照组、模型组(CCl_(4)诱导)及miRNA-218干预组(CCl_(4)+miRNA-218 agomir),每组20只。模型组及干预组小鼠腹腔注射20%CCl_(4)溶液(5 ml/kg,每周2次,持续8周),干预组同时通过尾静脉注射miRNA-218 agomir(10 nmol/kg,每周3次)。8周后取小鼠血液及肝脏组织,检测血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)水平,苏木精-伊红(HE)染色和Masson染色评估肝组织病理学变化及胶原沉积;实时荧光定量聚合酶链式反应(qPCR)检测miRNA-218及SLIT2、α-平滑肌肌动蛋白(α-SMA)、CollagenⅠ的mRNA表达水平;蛋白质免疫印迹法分析SLIT2、β-连环蛋白(β-catenin)及磷酸化GSK-3β(p-GSK-3β)蛋白水平;双荧光素酶报告基因验证miRNA-218与SLIT2的靶向关系。组间差异采用Student’s t检验。结果模型组小鼠血清ALT和AST水平高于对照组[ALT:(169.51±15.26)U/L比(35.23±4.84)U/L,t=9.451,P<0.01;AST:(241.33±20.16)U/L比(48.69±5.35)U/L,t=10.929,P<0.01],miRNA-218干预组ALT和AST水平低于模型组[ALT:(96.40±10.37)U/L比(169.51±15.26)U/L,t=6.032,P<0.01;AST:(134.17±18.62)U/L比(241.33±20.16)U/L,t=7.204,P<0.01]。Masson染色结果显示,模型组胶原沉积占比高于对照组[(28.76±3.58)%比(3.22±0.81)%,t=9.337,P<0.01];干预组胶原沉积面积占比低于模型组[(18.42±2.1)%比(28.76±3.58)%,t=5.034,P<0.05]。qPCR结果显示,模型组α-SMA和CollagenⅠ表达水平高于对照组(2.38±0.35比1.02±0.09,t=5.114,P<0.05;3.07±0.39比1.00±0.07,t=7.714,P<0.01),而干预组α-SMA和CollagenⅠ表达水平低于模型组(1.45±0.18比2.38±0.35,t=4.370,P<0.05;1.86±0.21比3.07±0.39,t=7.098,P<0.01)。双荧光素酶报告基因实验显示,miRNA-218 mimics+WT-SLIT2组荧光素酶活性低于miRNA-NC+WT-SLIT2组(0.58±0.04比1.00±0.12,t=5.114,P<0.05),而miRNA-218 mimics+MUT-SLIT2组荧光素酶活性相较于miRNA-NC+MUT-SLIT2组差异无统计学意义(1.14±0.15比0.99±0.07,t=0.967,P>0.05)。qPCR结果显示,模型组SLIT2 mRNA表达水平高于对照组(2.47±0.41比1.00±0.10,t=7.915,P<0.01),干预组SLIT2 mRNA表达水平低于模型组(1.33±0.15比2.47±0.41,t=5.174,P<0.05)。结论miRNA-218靶向抑SLIT2,阻β-catenin/GSK-3β通路,缓小鼠肝纤维化,为开发基于miRNA的靶向治疗策略提供了新机制依据。 展开更多
关键词 微小RNA-218 Slit导向配体2 肝纤维化 四氯化碳 β-连环蛋白/糖原合成酶激酶-3β通路
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SLIT3蛋白与绝经后骨质疏松症相关性及诊断意义
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作者 刘树华 曾韵杰 +8 位作者 杨东升 付赛 陈桐莹 杨贻富 林静 陈丹妍 杨磬雨 万雷 黄宏兴 《中国骨质疏松杂志》 北大核心 2025年第11期1561-1567,共7页
目的探讨狭缝引导配体3(slit guidance ligand 3,SLIT3)蛋白与绝经后骨质疏松症(postmenopausal osteoporosis,PMOP)发病的关联性及其作为诊断标志物的预测价值,并通过构建成骨细胞特异敲除SLIT3基因的小鼠模型,验证SLIT3对骨代谢的影... 目的探讨狭缝引导配体3(slit guidance ligand 3,SLIT3)蛋白与绝经后骨质疏松症(postmenopausal osteoporosis,PMOP)发病的关联性及其作为诊断标志物的预测价值,并通过构建成骨细胞特异敲除SLIT3基因的小鼠模型,验证SLIT3对骨代谢的影响。方法前瞻性病例对照研究和动物模型实验相结合。首先,收集294例绝经后患者的临床资料及血清,根据骨密度结果分为PMOP组(232例)和非骨质疏松症组(NOP,62例),测定患者血清中SLIT3、Osterix、RUNX2、β-CTX、TP1NP、N-MID OC的含量。比较两组受试者的基线数据及以上研究指标的表达水平;分析SLIT3与骨密度(bone mineral density,BMD)、OSX、RUNX2及骨转换指标(β-CTX、TP1NP、N-MID OC)的相关关系;纳入各项指标分析PMOP的危险因素;通过随机森林算法及构建受试者工作特征曲线进一步评价SLIT3对PMOP的预测效能。为了进一步验证SLIT3在骨代谢中的作用,构建成骨细胞特异性敲除SLIT3基因(SLIT3 flox/flox,BGLAP-cre)的C57BL/6小鼠,分为假手术组及去卵巢模型组,构建去卵巢模型,分析SLIT3对骨微结构的影响。结果与PMOP组患者相比,NOP组体重、腰椎平均骨密度、SLIT3、OSX、RUNX2蛋白水平更高,结果存在统计学差异。Spearman相关分析表明SLIT3蛋白水平与BMD、RUNX2、OSX蛋白水平均呈正相关关系(P<0.001);Logistic回归分析提示SLIT3水平是PMOP发生的独立危险因素。随机森林算法显示SLIT3蛋白水平在PMOP的发生中具有重要意义。同时ROC曲线证实SLIT3对PMOP具有良好的预测价值(AUC=0.857,95%CI:0.794~0.920,P<0.001)。动物实验证明,与Sham SLIT3 fl/fl组相比,OVX SLIT3 fl/fl组BV/TV、Tb.N更低而Tb.Sp和SMI指数明显增加(P<0.05);同时不论是假手术组还是去卵巢模型组,在组内与SLIT3 fl/fl组对照时,SLIT3 fl/fl;BGLAP-cre组全身骨密度和BV/TV更低,差异具有统计学意义(P<0.05)。结论血清中SLIT3蛋白水平可用于评估体内骨形成状态,SLIT3蛋白水平是PMOP的独立危险因素,且具有良好的预测作用,能为PMOP的预防及治疗提供一定的临床监测依据,临床患者若血清中SLIT3含量<2.32 ng/mL,则需警惕PMOP的发生。成骨细胞来源的SLIT3对维持骨量具有重要意义。 展开更多
关键词 骨质疏松症 狭缝引导配体3 疾病预测 绝经后女性 SLIT3基因敲除小鼠
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A Biomimetic Stress Field Modulation Strategy Inspired by Scorpion Compound Slit Sensilla Enabled High-Accuracy and Low-Power Positioning Sensor for Identifying the Load Incident Angles
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作者 Junqiu Zhang Yu Chen +11 位作者 Haoran Li Jiqi Gao Xiangbo Gu Jiachao Wu Xiaojing Qin Kejun Wang Bin Zhu Daobing Chen Tao Sun Jianhua Fan Zhiwu Han Luquan Ren 《Journal of Bionic Engineering》 2025年第2期727-738,共12页
Numerous arthropods evolve and optimize sensory systems, enabling them to effectively adapt complex and competitive habitats. Typically, scorpions can precisely perceive the prey location with the lowest metabolic rat... Numerous arthropods evolve and optimize sensory systems, enabling them to effectively adapt complex and competitive habitats. Typically, scorpions can precisely perceive the prey location with the lowest metabolic rate among invertebrates. This biological phenomenon contrasts sharply with engineered systems, which generally associates high accuracy with substantial energy consumption. Inspired by the Scorpion Compound Slit Sensilla (SCSS) with a stress field modulation strategy, a bionic positioning sensor with superior precision and minimal power consumption is developed for the first time, which utilizes the particular Minimum Positioning Units (MPUs) to efficiently locate vibration signals. The single MPU of the SCSS can recognize the direction of collinear loads by regulating the stress field distribution and further, the coupling action of three MPUs can realize all-angle vibration monitoring in plane. Experiments demonstrate that the bionic positioning sensor achieves 1.43 degrees of angle-error-free accuracy without additional energy supply. As a proof of concept, two bionic positioning sensors and machine learning algorithm are integrated to provide centimeter (cm)-accuracy target localization, ideally suited for the man-machine interaction. The novel design offers a new mechanism for the design of traditional positioning devices, improving precision and efficiency in both the meta-universe and real-world Internet-connected systems. 展开更多
关键词 Scorpion Compound Slit Sensilla(SCSS) Vibration Source Stress Field Modulation Strategy High-accuracy and low-power Positioning Sensor Minimum Positioning Unit(MPU)
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The Electromagnetic Problems of a Cylinder or Elliptic Cylinder with Infinite Axial Slits and Others
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作者 Wan Lixi directed by Gao Yougang 《The Journal of China Universities of Posts and Telecommunications》 EI CSCD 1998年第1期71-71,75,共2页
The electromagnetic scattering, leaking and coupling from a cylinder with an infinite axial slit have been investigated by many authors. However, they have not obtained strict closed form solutions. Almost all of the... The electromagnetic scattering, leaking and coupling from a cylinder with an infinite axial slit have been investigated by many authors. However, they have not obtained strict closed form solutions. Almost all of the papers only reported the method for the simplest problems. For slight complicated problems, such as the scattering, leaking or coupling from a cylinder with infinite axial slits or with arbitrary width of axial slits, the former authors have only discussed formally or even not discussed because their methods were inconvenient. In addition, the problems of the EM scattering, leaking or coupling from an elliptic cylinder with infinite axial slits, which can be used wildly in practice based on its easily variable shape, have not be solved. It is imaginable that to solve these problems is more difficult, because many authors were unfamiliar with Mathieu Functions. Meanwhile we have not had a completed Mathieu Function system as the study for Mathieu Function have been processing aiming to establish a completed system just like Bessel Function system. However, if people make use of some method and technique as well as the knowledge of Bessel Functions, the problem can be studied. In this theory, the author has studied strictly the electromagnetic problems (scattering, leaking and coupling) of an elliptic cylinder as well as a cylinder with infinite axial slits, and obtained strict closed form solutions. The train of thought is: (1) elliptic Cylinder by using separation of variables in elliptic coordinates to find the field expressions in each domain, matching them on boundary to obtain a dual series equation system, then letting the convenient auxiliary functions to change the dual series equation system to integral equations, making use of WKBJ Method to draw the singular kernel from the integral equations, we finally obtain a singular integral equations to be able to be solved by the theory of singular integral equation. (2) cylinder by using separation of variables method in cylinder coordinates to find the field expressions in each domain and matching them on the boundary, we can obtain a dual series equations system, then letting auxiliary functions to change the dual series equations to integral equations. The singular integral equation can be obtained utilizing asymptotic representation of Bessel or Hankle Function. Then the strict closed form solutions can be obtained. 展开更多
关键词 electromagnetic scattering electromagnetic leaking electromagnetic leaking axial slit
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miR-429调控SLIT2对前列腺癌细胞迁移及侵袭的影响
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作者 郭小勇 陈晓峰 +3 位作者 孙龙飞 张威 王栋 杜宇峰 《现代泌尿生殖肿瘤杂志》 2024年第5期291-297,共7页
目的 探讨微小RNA-429(miR-42 9)通过调控狭缝引导配体2(SLIT2)对前列腺癌细胞迁移及侵袭的影响。方法 实时荧光定量PCR(q RT-PCR)法测定人前列腺上皮细胞、人前列腺癌细胞22RV1、VCa P、C4-2、DU145、LNCa P、PC-3细胞株中mir-429的表... 目的 探讨微小RNA-429(miR-42 9)通过调控狭缝引导配体2(SLIT2)对前列腺癌细胞迁移及侵袭的影响。方法 实时荧光定量PCR(q RT-PCR)法测定人前列腺上皮细胞、人前列腺癌细胞22RV1、VCa P、C4-2、DU145、LNCa P、PC-3细胞株中mir-429的表达差异;将22RV1前列腺癌细胞分为空白对照组、miR-429 NC组、miR-429 inhibitor组、pc DNA组、pc-SLIT2组、miR-429inhibitor+sh-NC组和miR-429 inhibitor+sh-SLIT2组,测定各组miR-429及SLIT2的表达,双荧光素酶报告实验检测miR-429与SLIT2的靶向关系,Transwell小室实验检测细胞侵袭能力,划痕实验检测细胞迁移能力,Western blot检测细胞迁移相关蛋白(MMP-2、MMP-9)表达情况。结果 与人前列腺上皮细胞相比,22RV1、VCa P、C4-2、DU145、LNCa P、PC-3细胞中miR-429表达水平显著升高(P<0.05),其中22RV1前列腺癌细胞miR-429表达水平较高,因此选择22RV1前列腺癌细胞用于后续研究。荧光素酶报告结果显示,SLIT2与miR-429存在靶向关系;与空白对照组相比,miR-429 inhibitor组、miR-429 inhibitor+sh-SLIT2组22RV1前列腺癌细胞中miR-429 m RNA表达水平显著降低(P <0.05),pc-SLIT2组miR-429 m RNA表达水平差异无统计学意义(P>0.05);miR-429 inhibitor组、pc-SLIT2组、miR-429 inhibitor+sh-SLIT2组细胞侵袭、迁移能力、MMP-2、MMP-9蛋白表达显著降低(P<0.05),SLIT2蛋白表达显著升高(P<0.05);与miR-429inhibitor组相比,miR-429 inhibitor+sh-SLIT2组22RV1前列腺癌细胞侵袭、迁移能力、MMP-2、MMP-9蛋白表达显著升高(P<0.05)。结论 低表达miR-429可抑制22RV1前列腺癌细胞的侵袭及迁移能力,可能是通过上调SLIT2实现的。 展开更多
关键词 微小RNA-429 SLIT2 前列腺癌 迁移 侵袭
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毛兰素调节Slit2/Robo1信号通路对糖尿病肾病模型大鼠肾小球内皮细胞血管生成的影响 被引量:2
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作者 张菊云 陈绵雄 +1 位作者 华炳红 蒙绪标 《中国药理学与毒理学杂志》 CAS 北大核心 2024年第3期177-182,共6页
目的 探讨毛兰素对糖尿病肾病(DN)模型大鼠肾小球内皮细胞血管生成的影响及slit同源蛋白2(Slit2)/轴突导向受体蛋白1(Robo1)信号通路的作用。方法 采用高糖高脂饲料持续饲养雄性SD大鼠8周,ip给予链脲佐菌素35 mg·kg^(-1)制备DN大... 目的 探讨毛兰素对糖尿病肾病(DN)模型大鼠肾小球内皮细胞血管生成的影响及slit同源蛋白2(Slit2)/轴突导向受体蛋白1(Robo1)信号通路的作用。方法 采用高糖高脂饲料持续饲养雄性SD大鼠8周,ip给予链脲佐菌素35 mg·kg^(-1)制备DN大鼠模型。将DN模型大鼠分为模型组及模型+毛兰素10,20和40 mg·kg^(-1)组(ig给予毛兰素,持续8周),每组10只,同时设置正常对照组(ig给予0.5%羟甲基纤维素钠,持续8周)。尿蛋白定量试剂盒测定大鼠24 h尿蛋白水平,全自动生化分析仪检测血清空腹血糖(FPG)和血肌酐(Scr)水平,PAS染色观察大鼠肾组织病理变化,免疫荧光法检测肾组织血小板内皮细胞黏附分子31(CD31)和足细胞标志蛋白(PCX)表达水平,Western印迹法检测肾组织Slit2和Robo1蛋白表达。结果 与正常对照组比较,模型组大鼠FPG、Scr和24 h尿蛋白水平以及肾组织CD31、Slit2和Robo1蛋白表达显著升高(P<0.01);肾组织新增肾小球毛细血管,并出现肾小球肥大和系膜面积扩张,存在非管状CD31染色,缺少相邻PCX染色,以及部分CD31管状区域染色也缺乏相邻的PCX染色。与模型组比较,模型+毛兰素10,20和40 mg·kg^(-1)组大鼠FPG、Scr和24 h尿蛋白水平及肾组织CD31、Slit2和Robo1蛋白表达显著降低(P<0.05,P<0.01);肾组织新增肾小球毛细血管、肾小球肥大和系膜面积扩张现象减轻,CD31管状区域染色与PCX足细胞区域染色基本相邻。结论 毛兰素可能通过抑制Slit2/Robo1信号通路而抑制DN模型大鼠肾小球内皮细胞血管生成。 展开更多
关键词 毛兰素 Slit同源蛋白2 轴突导向受体蛋白1 糖尿病肾病 肾小球内皮细胞 血管生成
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慢性牙周炎伴咬合创伤患者龈沟液Sclerostin、SLIT3与牙周临床指标和RANKL-OPG系统的相关性研究 被引量:2
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作者 秦高伟 邱丽娜 +2 位作者 姜智雯 张思瑶 李慧 《现代生物医学进展》 CAS 2024年第1期83-86,105,共5页
目的:探讨慢性牙周炎伴咬合创伤患者龈沟液骨硬化蛋白(Sclerostin)、神经轴突导向因子3(SLIT3)与牙周临床指标和核因子-κb受体活化因子配体(RANKL)-骨保护蛋白(OPG)系统的相关性。方法:选择2021年1月至2023年1月我院收治的123例慢性牙... 目的:探讨慢性牙周炎伴咬合创伤患者龈沟液骨硬化蛋白(Sclerostin)、神经轴突导向因子3(SLIT3)与牙周临床指标和核因子-κb受体活化因子配体(RANKL)-骨保护蛋白(OPG)系统的相关性。方法:选择2021年1月至2023年1月我院收治的123例慢性牙周炎伴咬合创伤患者(创伤组)和101例不伴咬合创伤的慢性牙周炎患者(对照组)。治疗前,检测龈沟液中Sclerostin、SLIT3、RANKL、OPG水平,计算RANKL/OPG比值,评估临床牙周指标出血指数(SBI)、菌斑指数(PLI)、探诊深度(PD)、附着丧失(AL)。分析Sclerostin、SLIT3与牙周临床指标、RANKL、OPG、RANKL/OPG之间的相关性。结果:创伤组治疗前龈沟液中Sclerostin、SLIT3、RANKL水平,RANKL/OPG,PLI、SBI、AL、PD高于对照组(P<0.05),OPG低于对照组(P<0.05)。创伤组治疗前龈沟液中Sclerostin、SLIT3与PLI、SBI、AL、PD、RANKL、RANKL/OPG呈正相关(P<0.05),与OPG呈负相关(P<0.05)。结论:慢性牙周炎伴咬合创伤患者龈沟液中Sclerostin、SLIT3水平显著增高,且与牙周临床指标异常、RANKL/OPG增加有关。 展开更多
关键词 慢性牙周炎 咬合创伤 龈沟液 SCLEROSTIN SLIT3 牙周临床指标 RANKL-OPG系统
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