针对单一目标特征在复杂场景下难以实现有效的跟踪问题,提出了一种边缘纹理与颜色特征相融合的新方法。将Sobel算子与局部二值模式算子相结合,得到一种新的边缘纹理SLBP(Sobel Local Binary Pattern)特征提取方法,并与HSV(Hue,Saturatio...针对单一目标特征在复杂场景下难以实现有效的跟踪问题,提出了一种边缘纹理与颜色特征相融合的新方法。将Sobel算子与局部二值模式算子相结合,得到一种新的边缘纹理SLBP(Sobel Local Binary Pattern)特征提取方法,并与HSV(Hue,Saturation,Value)颜色特征融合应用于粒子滤波框架的视频目标跟踪。实验结果表明:本文提出的SLBP+HSV特征融合方法能够克服视频中光照变化、目标遮挡等复杂背景影响的问题,提高跟踪的精确度。展开更多
The-1 programmed ribosomal frameshifting(-1 PRF)in severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)is crucial for keeping the balance between pp1a and pp1ab polyproteins.To date,the host factors influencing...The-1 programmed ribosomal frameshifting(-1 PRF)in severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)is crucial for keeping the balance between pp1a and pp1ab polyproteins.To date,the host factors influencing this process remain poorly understood.Using RNA pull-down assays combined with mass spectrometry screening,we discovered five host proteins interacting with-1 PRF RNA,including Stem Loop Binding Protein(SLBP).Our findings revealed that SLBP overexpression enhanced frameshifting and promoted viral replication.Moreover,the interaction between SLBP and-1 PRF RNA was predicted using the PrismNet deep learning tool,which calculated a high binding probability of 0.922.Using Electrophoretic Mobility Shift Assays(EMSAs)and RNA pull down assays,our findings demonstrated SLBP’s direct binding to the SARS-CoV-2 genome,with preferential affinity for the stem loop 3 region of the-1 PRF RNA.Using smFISH assays,we further confirmed their physical colocalization.The role of SLBP in promoting frameshifting was verified using an in vitro translation system.Further investigation showed that SLBP deletions reshaped the host factor pattern around-1 PRF RNA,diminishing interactions with FUBP3 and RPS3A while enhancing RPL10A binding.Together,our findings identify SLBP as a host protein that promotes SARS-CoV-2 frameshifting,highlighting its potential as a druggable target for COVID-19.展开更多
文摘针对单一目标特征在复杂场景下难以实现有效的跟踪问题,提出了一种边缘纹理与颜色特征相融合的新方法。将Sobel算子与局部二值模式算子相结合,得到一种新的边缘纹理SLBP(Sobel Local Binary Pattern)特征提取方法,并与HSV(Hue,Saturation,Value)颜色特征融合应用于粒子滤波框架的视频目标跟踪。实验结果表明:本文提出的SLBP+HSV特征融合方法能够克服视频中光照变化、目标遮挡等复杂背景影响的问题,提高跟踪的精确度。
基金supported by the Major Program of the National Natural Science Foundation of China(No.82394462)the Foundation for Innovative Research Groups of the National Natural Science Foundation of China(82221004)+3 种基金State Key Laboratory Special Fund(2060204)the Yunnan Key R&D Project(202103AQ100001)National Key R&D Program of China(2020YFA0707602,2021YFC230170402,2021YFC0864600,2021YFC0863300)Chinese Academy of Medical Sciences Innovation Fund for Medical Sciences(2023-I2M-2-001,2021-I2M-1-038,2021-I2M-1-039).
文摘The-1 programmed ribosomal frameshifting(-1 PRF)in severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)is crucial for keeping the balance between pp1a and pp1ab polyproteins.To date,the host factors influencing this process remain poorly understood.Using RNA pull-down assays combined with mass spectrometry screening,we discovered five host proteins interacting with-1 PRF RNA,including Stem Loop Binding Protein(SLBP).Our findings revealed that SLBP overexpression enhanced frameshifting and promoted viral replication.Moreover,the interaction between SLBP and-1 PRF RNA was predicted using the PrismNet deep learning tool,which calculated a high binding probability of 0.922.Using Electrophoretic Mobility Shift Assays(EMSAs)and RNA pull down assays,our findings demonstrated SLBP’s direct binding to the SARS-CoV-2 genome,with preferential affinity for the stem loop 3 region of the-1 PRF RNA.Using smFISH assays,we further confirmed their physical colocalization.The role of SLBP in promoting frameshifting was verified using an in vitro translation system.Further investigation showed that SLBP deletions reshaped the host factor pattern around-1 PRF RNA,diminishing interactions with FUBP3 and RPS3A while enhancing RPL10A binding.Together,our findings identify SLBP as a host protein that promotes SARS-CoV-2 frameshifting,highlighting its potential as a druggable target for COVID-19.
