This paper presents a quantization skipping method for H. 264/AVC video coding standard. In order to reduce the conputational-cost of quantization process coming from integer discrete cosine transform of H. 264/AVC, a...This paper presents a quantization skipping method for H. 264/AVC video coding standard. In order to reduce the conputational-cost of quantization process coming from integer discrete cosine transform of H. 264/AVC, a quantization skipping condition is derived by the analysis of integer transform and quantization procedures. The expeerimental results show that the proposed algorithm has the capability to reduce the computional cost about 10% -2.5%.展开更多
Modeling and simulation results of a pulse skipping modulated buck converter for applications involving a source with widely varying voltage conditions with loads requiring constant voltage from full load down to no l...Modeling and simulation results of a pulse skipping modulated buck converter for applications involving a source with widely varying voltage conditions with loads requiring constant voltage from full load down to no load is presented. The pulses applied to the switch are blocked or released on output voltage crossing a predetermined value. The regulator worked satisfactorily over a wide input voltage range with good transient response but with higher ripple content. Input current spectrum indicates a good EMI performance with crowding of components at audio frequency range for the se-lected switching frequency.展开更多
This paper adopts the research methods such as literature material method, questionnaire investigation method, expert interview method and mathematical statistics to investigate and study the conditions of fancy rope ...This paper adopts the research methods such as literature material method, questionnaire investigation method, expert interview method and mathematical statistics to investigate and study the conditions of fancy rope skipping curriculum in 22 universities in Sichuan. The research shows that the university teachers highly recognize the fancy rope skipping curriculum; construction of teaching resources should be strengthened; cultivation of the professional teaching staffs should be strengthened; in terms of teaching materials, more premium teaching materials with high pertinence and professionalism are needed; the promotion and implementation of the curriculum system of fancy rope skipping should be boosted in different steps and stages; cultivation of the professional teachers should be enlarged; related scientific research of the curriculum system of fancy rope skipping should be strengthened.展开更多
An in-depth study of the complicated trajectory characteristics of skipping stones is carried out in the present work.A three-dimensional numerical simulation validated by acquiring good agreement with experimental re...An in-depth study of the complicated trajectory characteristics of skipping stones is carried out in the present work.A three-dimensional numerical simulation validated by acquiring good agreement with experimental results is established.It is devoted to illustrating five different types of motion responses after the stone impacts the water surface in the o-xy plane,including“dive”,“hydroplaning trout”,“hydroplaning skip”,“stable skip”,and“skipping trout”.Then,the lateral deviations are investigated quantitatively based on dimensionless parameter sin(α+β)cosαin the o-yz plane.Steady interval and linear interval are divided for lateral deviation Z1/D and Z2/D based on the values of(α+β)andα,respectively.The results reveal that(1)Z1/D increases almost linearly with the increasing sin(α+β)cosαat different slopes in different(α+β)intervals;(2)Z2/D increases almost linearly with the increasing sin(α+β)cosαat different slopes in differentαintervals;(3)in linear interval,numerical lateral deviations are much larger than the fitting values at pointsβ/α≥4.5,and much smaller than the fitting values at points 2.3≤β/α<4.3.Finally,a theoretical approach is proposed to predict the maximum immersion depth of trailing edge point P.展开更多
Background Unhealthy lifestyles have a considerable impact on the incidence of dementia.Skipping breakfast disturbs energy homeostasis and impairs brain function.In this study,we investigated the association between b...Background Unhealthy lifestyles have a considerable impact on the incidence of dementia.Skipping breakfast disturbs energy homeostasis and impairs brain function.In this study,we investigated the association between breakfast skipping and cognitive performance among community-dwelling adults.Methods We recruited 859 community-dwelling adults aged≥60 years from January 1 to December 31,2021.Participants’sociodemographic information and breakfast skipping habits were self-reported.Participants were followed up for 36 months and cognitive function was assessed using the Mini-Mental State Examination(MMSE)with an interval of 18 months.Trajectories of cognitive change were compared between individuals with and without breakfast skipping.To reduce the risk of bias owing to unmatched sample sizes between the groups,we conducted 1:1 propensity score matching(PSM)based on age,sex,education level,and ApoE genotype.Results At baseline and 18-month follow-up,no difference was found in MMSE scores between participants with and without breakfast skipping.However,those who habitually skipped breakfast had significantly lower MMSE scores than those who did not at 36-month follow-up.Individuals with habitual breakfast skipping had a steeper rate of cognitive decline than those without habitual breakfast skipping during follow-up.Breakfast skipping was a risk factor for longitudinal cognitive decline,defined as a decrease in MMSE scores of≥3,adjusted for age,sex,education,body mass index,ApoEε4 carrier status,hypertension,diabetes,and hyperlipidemia.At the last follow-up,participants who habitually skipped breakfast had significantly higher levels of ptau181 and NfL than those who did not.In the PSM cohort,similar findings were obtained regarding cognitive trajectories and plasma biomarkers.Conclusion Breakfast skipping was linked to an increased risk of long-term cognitive decline and neurodegeneration among older adults.The link between unhealthy dietary habits and cognitive decline may be attributed to a deficiency in neurorestoration resulting from inadequate energy consumption.展开更多
Tepotinib is a key drug for cancer patients with mesenchymal-epithelial transition receptor tyrosine kinase proto-oncogene(MET)exon 14 skipping mutation.However,its bioavailability in the cerebrospinal fluid(CSF)in hu...Tepotinib is a key drug for cancer patients with mesenchymal-epithelial transition receptor tyrosine kinase proto-oncogene(MET)exon 14 skipping mutation.However,its bioavailability in the cerebrospinal fluid(CSF)in humans has not been fully elucidated.Moreover,information about the efficacy of tepotinib in patients with leptomeningeal metastasis is limited.Here,we present the case of a 56-year-old man who was diagnosed with lung adenocarcinoma with MET exon 14 skipping mutation.He was urgently hospitalized due to leptomeningeal metastasis.We administered tepotinib 500 mg/day as the second-line therapy and observed improvement in leptomeningeal metastasis and performance status.The tepotinib concentrations reached 1,648 ng/mL in the plasma and 30.6 ng/mL in the CSF,with a penetration rate(CSF/plasma)of 1.83%.These demonstrate tepotinib could achieve a high rate of central nervous system transition and could be effective against leptomeningeal metastasis.展开更多
Summary We isolated a mutant showing perturbations in the development of male and female floral organs and fruits. Analysis of the single nucleotide polyTnorphisms from bulked F2 pools identified the causative variant...Summary We isolated a mutant showing perturbations in the development of male and female floral organs and fruits. Analysis of the single nucleotide polyTnorphisms from bulked F2 pools identified the causative variant occurring in Csa4G126690. Csa4G126690 shows high homol- ogy to Arobidopsis 5EPALLATA2 （SEP2） thus being desig- nated CsSEP2. The causative variant was located on the splicing site of CsSEP2, resulting in the skipping of exon 6 and abolishment of the transcriptional activity. Our data suggest that CsSEP2 is involved in the floral organ and fruits development by conferring transcriptional activity.展开更多
The error-prone V(D)J recombination process generates considerable amounts of nonproductive immunoglobulin(Ig)pre-mRNAs.We recently demonstrated that aberrant Ig chains lacking variable(V)domains can be produced after...The error-prone V(D)J recombination process generates considerable amounts of nonproductive immunoglobulin(Ig)pre-mRNAs.We recently demonstrated that aberrant Ig chains lacking variable(V)domains can be produced after nonsense-associated altered splicing(NAS)events.Remarkably,the expression of these truncated Ig polypeptides heightens endoplasmic reticulum stress and shortens plasma cell(PC)lifespan.Many questions remain regarding the molecular mechanisms underlying this new truncated Ig exclusion(TIE-)checkpoint and its restriction to the ultimate stage of B-cell differentiation.To address these issues,we evaluated the extent of NAS of Ig pre-mRNAs using an Ig heavy chain(IgH)knock-in model that allows for uncoupling of V exon skipping from TIE-induced apoptosis.We found high levels of V exon skipping in PCs compared with B cells,and this skipping was correlated with a biallelic boost in IgH transcription during PC differentiation.Chromatin analysis further revealed that the skipped V exon turned into a pseudo-intron.Finally,we showed that hypertranscription of Ig genes facilitated V exon skipping upon passive administration of splice-switching antisense oligonucleotides(ASOs).Thus,V exon skipping is coupled to transcription and increases as PC differentiation proceeds,likely explaining the late occurrence of the TIE-checkpoint and opening new avenues for ASO-mediated strategies in PC disorders.展开更多
Gene fusions and MET alterations are rare and difficult to detect in plasma samples.The clinical detection efficacy of molecular residual disease(MRD)based on circulating tumor DNA(ctDNA)in patients with non-small cel...Gene fusions and MET alterations are rare and difficult to detect in plasma samples.The clinical detection efficacy of molecular residual disease(MRD)based on circulating tumor DNA(ctDNA)in patients with non-small cell lung cancer(NSCLC)with these mutations remains unknown.This prospective,non-intervention study recruited 49 patients with operable NSCLC with actionable gene fusions(ALK,ROS1,RET,and FGFR1),MET exon 14 skipping or de novo MET amplification.We analyzed 43 tumor tissues and 111 serial perioperative plasma samples using 1021-and 338-gene panels,respectively.Detectable MRD correlated with a significantly higher recurrence rate(P<0.001),yielding positive predictive values of 100%and 90.9%,and negative predictive values of 82.4%and 86.4%at landmark and longitudinal time points,respectively.Patients with detectable MRD showed reduced disease-free survival(DFS)compared to those with undetectable MRD(P<0.001).Patients who harbored tissue-derived fusion/MET alterations in their MRD had reduced DFS compared to those who did not(P=0.05).To our knowledge,this is the first comprehensive study on ctDNA-MRD clinical detection efficacy in operable NSCLC patients with gene fusions and MET alterations.Patients with detectable tissue-derived fusion/MET alterations in postoperative MRD had worse clinical outcomes.展开更多
We describe an efficient and easily applicable data deduplication framework with heuristic prediction based adaptive block skipping for the real-world dataset such as disk images to save deduplication related overhead...We describe an efficient and easily applicable data deduplication framework with heuristic prediction based adaptive block skipping for the real-world dataset such as disk images to save deduplication related overheads and improve deduplication throughput with good deduplication efficiency maintained. Under the framework, deduplication operations are skipped for data chunks determined as likely non-duplicates via heuristic prediction, in conjunction with a hit and matching extension process for duplication identification within skipped blocks and a hysteresis mechanism based hash indexing process to update the hash indices for the re-encountered skipped chunks. For performance evaluation, the proposed framework was integrated and implemented in the existing data domain and sparse indexing deduplication algorithms. The experimental results based on a real-world dataset of 1.0 TB disk images showed that the deduplication related overheads were significantly reduced with adaptive block skipping, leading to a 30%-80% improvement in deduplication throughput when deduplieation mctadata were stored on the disk for data domain, and 25%-40% RAM space saving with a 15%-20% improvement in deduplication throughput when an in-RAM sparse index was used in sparse indexing. In both cases, the corresponding deduplication ratios reduced were below 5%.展开更多
Alternative splicing(AS)has been shown to be frequently present in human tumors.Specifically,it has been observed in some experimental studies thatmulti-exon skipping(MES)events often appear in tumorous tissues.Prompt...Alternative splicing(AS)has been shown to be frequently present in human tumors.Specifically,it has been observed in some experimental studies thatmulti-exon skipping(MES)events often appear in tumorous tissues.Prompted by this observation,we conducted a genome-wide analysis of MES events to investigate their association with tumors.The results show that MES events are more likely associated with tumors than single-exon skipping(SES)and the degree of association increases with the num-ber of skipped exons.Furthermore,MES events are found to be less conserved than their SES counterparts,which provides additional evidence for our results because dis-ease-associated AS events should be eliminated during evolution.Interestingly,these differences still existed even after comparison of MES and SES events with similar-length skipped regions.These results demonstrate that MES events may be associated with tumors and suggest that MES isoforms might be useful in cancer diagnosis.展开更多
Alterations in the mesenchymal-epithelial transition factor(MET)gene are critical drivers of non-small cell lung cancer(NSCLC).In recent years advances in precision therapies targeting MET alterations have significant...Alterations in the mesenchymal-epithelial transition factor(MET)gene are critical drivers of non-small cell lung cancer(NSCLC).In recent years advances in precision therapies targeting MET alterations have significantly expanded treatment options for NSCLC patients.These alterations include MET exon 14 skipping mutations(MET exon 14 skipping),MET gene amplifications,MET point mutations(primarily kinase domain mutations),and MET protein overexpression.Accurate identification of these alterations and appropriate selection of patient populations and targeted therapies are essential for improving clinical outcomes.The East China Lung Cancer Group,Youth Committee(ECLUNG YOUNG,Yangtze River Delta Lung Cancer Cooperation Group)has synthesized insights from China’s innovative drug development landscape and clinical practice to formulate an expert consensus on the diagnosis and treatment of NSCLC patients with MET alterations.This consensus addresses key areas,such as optimal testing timing,testing methods,testing strategies,quality control measures,and treatment approaches.By offering standardized recommendations,this guidance aims to streamline diagnostic and therapeutic processes and enhance clinical decision-making for NSCLC with MET alterations.展开更多
The maneuverability and stealth of aerial-aquatic vehicles(AAVs)is of significant importance for future integrated air-sea combat missions.To improve the maneuverability and stealth of AAVs near the water surface,this...The maneuverability and stealth of aerial-aquatic vehicles(AAVs)is of significant importance for future integrated air-sea combat missions.To improve the maneuverability and stealth of AAVs near the water surface,this paper proposed a high-maneuverability skipping motion strategy for the tandem twin-rotor AAV,inspired by the motion behavior of the flying fish to avoid aquatic and aerial predators near the water surface.The novel tandem twin-rotor AAV was employed as the research subject and a strategybased ADRC control method for validation,comparing it with a strategy-based PID control method.The results indicate that both control methods enable the designed AAV to achieve high stealth and maneuverability near the water surface with robust control stability.The strategy-based ADRC control method exhibits a certain advantage in controlling height,pitch angle,and reducing impact force.This motion strategy will offer an inspiring approach for the practical application of AAVs to some extent.展开更多
Image inpainting refers to synthesizing missing content in an image based on known information to restore occluded or damaged regions,which is a typical manifestation of this trend.With the increasing complexity of im...Image inpainting refers to synthesizing missing content in an image based on known information to restore occluded or damaged regions,which is a typical manifestation of this trend.With the increasing complexity of image in tasks and the growth of data scale,existing deep learning methods still have some limitations.For example,they lack the ability to capture long-range dependencies and their performance in handling multi-scale image structures is suboptimal.To solve this problem,the paper proposes an image inpainting method based on the parallel dual-branch learnable Transformer network.The encoder of the proposed model generator consists of a dual-branch parallel structure with stacked CNN blocks and Transformer blocks,aiming to extract global and local feature information from images.Furthermore,a dual-branch fusion module is adopted to combine the features obtained from both branches.Additionally,a gated full-scale skip connection module is proposed to further enhance the coherence of the inpainting results and alleviate information loss.Finally,experimental results from the three public datasets demonstrate the superior performance of the proposed method.展开更多
Semantic segmentation plays a foundational role in biomedical image analysis, providing precise information about cellular, tissue, and organ structures in both biological and medical imaging modalities. Traditional a...Semantic segmentation plays a foundational role in biomedical image analysis, providing precise information about cellular, tissue, and organ structures in both biological and medical imaging modalities. Traditional approaches often fail in the face of challenges such as low contrast, morphological variability, and densely packed structures. Recent advancements in deep learning have transformed segmentation capabilities through the integration of fine-scale detail preservation, coarse-scale contextual modeling, and multi-scale feature fusion. This work provides a comprehensive analysis of state-of-the-art deep learning models, including U-Net variants, attention-based frameworks, and Transformer-integrated networks, highlighting innovations that improve accuracy, generalizability, and computational efficiency. Key architectural components such as convolution operations, shallow and deep blocks, skip connections, and hybrid encoders are examined for their roles in enhancing spatial representation and semantic consistency. We further discuss the importance of hierarchical and instance-aware segmentation and annotation in interpreting complex biological scenes and multiplexed medical images. By bridging methodological developments with diverse application domains, this paper outlines current trends and future directions for semantic segmentation, emphasizing its critical role in facilitating annotation, diagnosis, and discovery in biomedical research.展开更多
基金supported by the Seoul Future Contents Convergence(SFCC)Cluster established by Seoul R&BD Program
文摘This paper presents a quantization skipping method for H. 264/AVC video coding standard. In order to reduce the conputational-cost of quantization process coming from integer discrete cosine transform of H. 264/AVC, a quantization skipping condition is derived by the analysis of integer transform and quantization procedures. The expeerimental results show that the proposed algorithm has the capability to reduce the computional cost about 10% -2.5%.
文摘Modeling and simulation results of a pulse skipping modulated buck converter for applications involving a source with widely varying voltage conditions with loads requiring constant voltage from full load down to no load is presented. The pulses applied to the switch are blocked or released on output voltage crossing a predetermined value. The regulator worked satisfactorily over a wide input voltage range with good transient response but with higher ripple content. Input current spectrum indicates a good EMI performance with crowding of components at audio frequency range for the se-lected switching frequency.
文摘This paper adopts the research methods such as literature material method, questionnaire investigation method, expert interview method and mathematical statistics to investigate and study the conditions of fancy rope skipping curriculum in 22 universities in Sichuan. The research shows that the university teachers highly recognize the fancy rope skipping curriculum; construction of teaching resources should be strengthened; cultivation of the professional teaching staffs should be strengthened; in terms of teaching materials, more premium teaching materials with high pertinence and professionalism are needed; the promotion and implementation of the curriculum system of fancy rope skipping should be boosted in different steps and stages; cultivation of the professional teachers should be enlarged; related scientific research of the curriculum system of fancy rope skipping should be strengthened.
基金supported by the National Natural Science Foundation of China(Grant No.11972138).
文摘An in-depth study of the complicated trajectory characteristics of skipping stones is carried out in the present work.A three-dimensional numerical simulation validated by acquiring good agreement with experimental results is established.It is devoted to illustrating five different types of motion responses after the stone impacts the water surface in the o-xy plane,including“dive”,“hydroplaning trout”,“hydroplaning skip”,“stable skip”,and“skipping trout”.Then,the lateral deviations are investigated quantitatively based on dimensionless parameter sin(α+β)cosαin the o-yz plane.Steady interval and linear interval are divided for lateral deviation Z1/D and Z2/D based on the values of(α+β)andα,respectively.The results reveal that(1)Z1/D increases almost linearly with the increasing sin(α+β)cosαat different slopes in different(α+β)intervals;(2)Z2/D increases almost linearly with the increasing sin(α+β)cosαat different slopes in differentαintervals;(3)in linear interval,numerical lateral deviations are much larger than the fitting values at pointsβ/α≥4.5,and much smaller than the fitting values at points 2.3≤β/α<4.3.Finally,a theoretical approach is proposed to predict the maximum immersion depth of trailing edge point P.
文摘Background Unhealthy lifestyles have a considerable impact on the incidence of dementia.Skipping breakfast disturbs energy homeostasis and impairs brain function.In this study,we investigated the association between breakfast skipping and cognitive performance among community-dwelling adults.Methods We recruited 859 community-dwelling adults aged≥60 years from January 1 to December 31,2021.Participants’sociodemographic information and breakfast skipping habits were self-reported.Participants were followed up for 36 months and cognitive function was assessed using the Mini-Mental State Examination(MMSE)with an interval of 18 months.Trajectories of cognitive change were compared between individuals with and without breakfast skipping.To reduce the risk of bias owing to unmatched sample sizes between the groups,we conducted 1:1 propensity score matching(PSM)based on age,sex,education level,and ApoE genotype.Results At baseline and 18-month follow-up,no difference was found in MMSE scores between participants with and without breakfast skipping.However,those who habitually skipped breakfast had significantly lower MMSE scores than those who did not at 36-month follow-up.Individuals with habitual breakfast skipping had a steeper rate of cognitive decline than those without habitual breakfast skipping during follow-up.Breakfast skipping was a risk factor for longitudinal cognitive decline,defined as a decrease in MMSE scores of≥3,adjusted for age,sex,education,body mass index,ApoEε4 carrier status,hypertension,diabetes,and hyperlipidemia.At the last follow-up,participants who habitually skipped breakfast had significantly higher levels of ptau181 and NfL than those who did not.In the PSM cohort,similar findings were obtained regarding cognitive trajectories and plasma biomarkers.Conclusion Breakfast skipping was linked to an increased risk of long-term cognitive decline and neurodegeneration among older adults.The link between unhealthy dietary habits and cognitive decline may be attributed to a deficiency in neurorestoration resulting from inadequate energy consumption.
文摘Tepotinib is a key drug for cancer patients with mesenchymal-epithelial transition receptor tyrosine kinase proto-oncogene(MET)exon 14 skipping mutation.However,its bioavailability in the cerebrospinal fluid(CSF)in humans has not been fully elucidated.Moreover,information about the efficacy of tepotinib in patients with leptomeningeal metastasis is limited.Here,we present the case of a 56-year-old man who was diagnosed with lung adenocarcinoma with MET exon 14 skipping mutation.He was urgently hospitalized due to leptomeningeal metastasis.We administered tepotinib 500 mg/day as the second-line therapy and observed improvement in leptomeningeal metastasis and performance status.The tepotinib concentrations reached 1,648 ng/mL in the plasma and 30.6 ng/mL in the CSF,with a penetration rate(CSF/plasma)of 1.83%.These demonstrate tepotinib could achieve a high rate of central nervous system transition and could be effective against leptomeningeal metastasis.
基金supported by grants from the National Natural Science Foundation of China (31225025)the National Program on Key Basic Research Projects in China (2012CB113900)the China Postdoctoral Science Foundation (2015M581217)
文摘Summary We isolated a mutant showing perturbations in the development of male and female floral organs and fruits. Analysis of the single nucleotide polyTnorphisms from bulked F2 pools identified the causative variant occurring in Csa4G126690. Csa4G126690 shows high homol- ogy to Arobidopsis 5EPALLATA2 （SEP2） thus being desig- nated CsSEP2. The causative variant was located on the splicing site of CsSEP2, resulting in the skipping of exon 6 and abolishment of the transcriptional activity. Our data suggest that CsSEP2 is involved in the floral organ and fruits development by conferring transcriptional activity.
基金supported by grants from Fondation ARC(PJA 20161204724/PGA120150202338)INCa(PLBIO15-256)+3 种基金ANR(2017-CE15-0024-01)Ligue Contre le Cancer(comites Correze,Haute-Vienne)Fondation Française pour la Recherche contre le Myelome et les Gammapathies monoclonales(FFRMG)Comited’Organisation de la Recherche sur le Cancer du Limousin(CORC).
文摘The error-prone V(D)J recombination process generates considerable amounts of nonproductive immunoglobulin(Ig)pre-mRNAs.We recently demonstrated that aberrant Ig chains lacking variable(V)domains can be produced after nonsense-associated altered splicing(NAS)events.Remarkably,the expression of these truncated Ig polypeptides heightens endoplasmic reticulum stress and shortens plasma cell(PC)lifespan.Many questions remain regarding the molecular mechanisms underlying this new truncated Ig exclusion(TIE-)checkpoint and its restriction to the ultimate stage of B-cell differentiation.To address these issues,we evaluated the extent of NAS of Ig pre-mRNAs using an Ig heavy chain(IgH)knock-in model that allows for uncoupling of V exon skipping from TIE-induced apoptosis.We found high levels of V exon skipping in PCs compared with B cells,and this skipping was correlated with a biallelic boost in IgH transcription during PC differentiation.Chromatin analysis further revealed that the skipped V exon turned into a pseudo-intron.Finally,we showed that hypertranscription of Ig genes facilitated V exon skipping upon passive administration of splice-switching antisense oligonucleotides(ASOs).Thus,V exon skipping is coupled to transcription and increases as PC differentiation proceeds,likely explaining the late occurrence of the TIE-checkpoint and opening new avenues for ASO-mediated strategies in PC disorders.
基金supported by funding from the National Natural Science Foundation of China Major Joint Project on Key Scientific Issues of Lung Cancer(No.82241235)the National Natural Science Foundation of China(No.81872510)+3 种基金Guangdong Provincial People’s Hospital Young Talent Project(No.GDPPHYTP201902)Guangdong Basic and Applied Basic Research Foundation(No.2019B1515130002)High-level Hospital Construction Project(No.DFJH201801)Guangdong Provincial Key Laboratory of Translational Medicine in Lung Cancer(No.2017B030314120).
文摘Gene fusions and MET alterations are rare and difficult to detect in plasma samples.The clinical detection efficacy of molecular residual disease(MRD)based on circulating tumor DNA(ctDNA)in patients with non-small cell lung cancer(NSCLC)with these mutations remains unknown.This prospective,non-intervention study recruited 49 patients with operable NSCLC with actionable gene fusions(ALK,ROS1,RET,and FGFR1),MET exon 14 skipping or de novo MET amplification.We analyzed 43 tumor tissues and 111 serial perioperative plasma samples using 1021-and 338-gene panels,respectively.Detectable MRD correlated with a significantly higher recurrence rate(P<0.001),yielding positive predictive values of 100%and 90.9%,and negative predictive values of 82.4%and 86.4%at landmark and longitudinal time points,respectively.Patients with detectable MRD showed reduced disease-free survival(DFS)compared to those with undetectable MRD(P<0.001).Patients who harbored tissue-derived fusion/MET alterations in their MRD had reduced DFS compared to those who did not(P=0.05).To our knowledge,this is the first comprehensive study on ctDNA-MRD clinical detection efficacy in operable NSCLC patients with gene fusions and MET alterations.Patients with detectable tissue-derived fusion/MET alterations in postoperative MRD had worse clinical outcomes.
基金This work is supported by the National Science Fund for Distinguished Young Scholars of China under Grant No. 61125102 and the Key Program of National Natural Science Foundation of China under Grant No. 61133008.
文摘We describe an efficient and easily applicable data deduplication framework with heuristic prediction based adaptive block skipping for the real-world dataset such as disk images to save deduplication related overheads and improve deduplication throughput with good deduplication efficiency maintained. Under the framework, deduplication operations are skipped for data chunks determined as likely non-duplicates via heuristic prediction, in conjunction with a hit and matching extension process for duplication identification within skipped blocks and a hysteresis mechanism based hash indexing process to update the hash indices for the re-encountered skipped chunks. For performance evaluation, the proposed framework was integrated and implemented in the existing data domain and sparse indexing deduplication algorithms. The experimental results based on a real-world dataset of 1.0 TB disk images showed that the deduplication related overheads were significantly reduced with adaptive block skipping, leading to a 30%-80% improvement in deduplication throughput when deduplieation mctadata were stored on the disk for data domain, and 25%-40% RAM space saving with a 15%-20% improvement in deduplication throughput when an in-RAM sparse index was used in sparse indexing. In both cases, the corresponding deduplication ratios reduced were below 5%.
基金supported by the National Natural Science Foundation of China (Grant Nos.60775002,60572086).
文摘Alternative splicing(AS)has been shown to be frequently present in human tumors.Specifically,it has been observed in some experimental studies thatmulti-exon skipping(MES)events often appear in tumorous tissues.Prompted by this observation,we conducted a genome-wide analysis of MES events to investigate their association with tumors.The results show that MES events are more likely associated with tumors than single-exon skipping(SES)and the degree of association increases with the num-ber of skipped exons.Furthermore,MES events are found to be less conserved than their SES counterparts,which provides additional evidence for our results because dis-ease-associated AS events should be eliminated during evolution.Interestingly,these differences still existed even after comparison of MES and SES events with similar-length skipped regions.These results demonstrate that MES events may be associated with tumors and suggest that MES isoforms might be useful in cancer diagnosis.
文摘Alterations in the mesenchymal-epithelial transition factor(MET)gene are critical drivers of non-small cell lung cancer(NSCLC).In recent years advances in precision therapies targeting MET alterations have significantly expanded treatment options for NSCLC patients.These alterations include MET exon 14 skipping mutations(MET exon 14 skipping),MET gene amplifications,MET point mutations(primarily kinase domain mutations),and MET protein overexpression.Accurate identification of these alterations and appropriate selection of patient populations and targeted therapies are essential for improving clinical outcomes.The East China Lung Cancer Group,Youth Committee(ECLUNG YOUNG,Yangtze River Delta Lung Cancer Cooperation Group)has synthesized insights from China’s innovative drug development landscape and clinical practice to formulate an expert consensus on the diagnosis and treatment of NSCLC patients with MET alterations.This consensus addresses key areas,such as optimal testing timing,testing methods,testing strategies,quality control measures,and treatment approaches.By offering standardized recommendations,this guidance aims to streamline diagnostic and therapeutic processes and enhance clinical decision-making for NSCLC with MET alterations.
基金supported by Southern Marine Science and Guangdong Laboratory(Zhuhai)(Grant No.SML2023SP229)。
文摘The maneuverability and stealth of aerial-aquatic vehicles(AAVs)is of significant importance for future integrated air-sea combat missions.To improve the maneuverability and stealth of AAVs near the water surface,this paper proposed a high-maneuverability skipping motion strategy for the tandem twin-rotor AAV,inspired by the motion behavior of the flying fish to avoid aquatic and aerial predators near the water surface.The novel tandem twin-rotor AAV was employed as the research subject and a strategybased ADRC control method for validation,comparing it with a strategy-based PID control method.The results indicate that both control methods enable the designed AAV to achieve high stealth and maneuverability near the water surface with robust control stability.The strategy-based ADRC control method exhibits a certain advantage in controlling height,pitch angle,and reducing impact force.This motion strategy will offer an inspiring approach for the practical application of AAVs to some extent.
基金supported by Scientific Research Fund of Hunan Provincial Natural Science Foundation under Grant 20231J60257Hunan Provincial Engineering Research Center for Intelligent Rehabilitation Robotics and Assistive Equipment under Grant 2025SH501Inha University and Design of a Conflict Detection and Validation Tool under Grant HX2024123.
文摘Image inpainting refers to synthesizing missing content in an image based on known information to restore occluded or damaged regions,which is a typical manifestation of this trend.With the increasing complexity of image in tasks and the growth of data scale,existing deep learning methods still have some limitations.For example,they lack the ability to capture long-range dependencies and their performance in handling multi-scale image structures is suboptimal.To solve this problem,the paper proposes an image inpainting method based on the parallel dual-branch learnable Transformer network.The encoder of the proposed model generator consists of a dual-branch parallel structure with stacked CNN blocks and Transformer blocks,aiming to extract global and local feature information from images.Furthermore,a dual-branch fusion module is adopted to combine the features obtained from both branches.Additionally,a gated full-scale skip connection module is proposed to further enhance the coherence of the inpainting results and alleviate information loss.Finally,experimental results from the three public datasets demonstrate the superior performance of the proposed method.
基金Open Access funding provided by the National Institutes of Health(NIH)The funding for this project was provided by NCATS Intramural Fund.
文摘Semantic segmentation plays a foundational role in biomedical image analysis, providing precise information about cellular, tissue, and organ structures in both biological and medical imaging modalities. Traditional approaches often fail in the face of challenges such as low contrast, morphological variability, and densely packed structures. Recent advancements in deep learning have transformed segmentation capabilities through the integration of fine-scale detail preservation, coarse-scale contextual modeling, and multi-scale feature fusion. This work provides a comprehensive analysis of state-of-the-art deep learning models, including U-Net variants, attention-based frameworks, and Transformer-integrated networks, highlighting innovations that improve accuracy, generalizability, and computational efficiency. Key architectural components such as convolution operations, shallow and deep blocks, skip connections, and hybrid encoders are examined for their roles in enhancing spatial representation and semantic consistency. We further discuss the importance of hierarchical and instance-aware segmentation and annotation in interpreting complex biological scenes and multiplexed medical images. By bridging methodological developments with diverse application domains, this paper outlines current trends and future directions for semantic segmentation, emphasizing its critical role in facilitating annotation, diagnosis, and discovery in biomedical research.
