跳频码序列的性质直接影响着跳频通信的性能 ,计数式跳历 To D( Time of Day)跳频码序列的产生算法是对计数式 To D的分组加密变换。基于这种认识 ,提出了用分组加密 Skipjack算法来构成跳频码序列的产生算法 ,分析和测试表明所得的跳...跳频码序列的性质直接影响着跳频通信的性能 ,计数式跳历 To D( Time of Day)跳频码序列的产生算法是对计数式 To D的分组加密变换。基于这种认识 ,提出了用分组加密 Skipjack算法来构成跳频码序列的产生算法 ,分析和测试表明所得的跳频码序列在均匀性、相关性、复杂性、游程和频隙滞留等方面都能满足跳频码序列的要求 ;在 FPGA上综合的结果说明 ,该算法是一个便于芯片实现的、可实用的跳频码发生器方案。展开更多
In the western and central Pacific Ocean,upper strata waters exhibit highly dynamic oceanographic features under ENSO variability.This has been proved to be responsible for the dynamic change of both abundance and zon...In the western and central Pacific Ocean,upper strata waters exhibit highly dynamic oceanographic features under ENSO variability.This has been proved to be responsible for the dynamic change of both abundance and zonal distribution of skipjack tuna(Katsuwonus pelamis).Although causality has been suggested by researchers using physical-biological interaction models,cumulative evidence needs to be obtained and the tenability of assertion needs to be tested from an ecological habitat perspective,based on fisheries data.For purse seine fishery,the use of catch per unit effort(CPUE)as an indication of the abundance is confusing because of technical improvements over the whole exploitation history and unbalanced individual fishing characteristic of vessels.It is particularly interesting to discriminate between habitat characteristics in comparative scenarios of CPUE application.This study identified habitat traits based on a series of oceanographic factors from a global ocean reanalysis model.A comparison was conducted between two habitat models based on unprocessed purse seine CPUE and standardized CPUE considering fishing characteristics.The results suggest that standardized CPUE could model the regular zonal shift of habitat compatible with the observed fishing efforts transfer,and achieved better prediction capacity than unprocessed CPUE.Furthermore,the habitat of skipjack tuna was also characterized and linked with surface and subsurface thermal environment,ocean current,dissolved oxygen,biotic environment,and ENSO variability.The monthly-averaged habitat suitable index,derived from the optimal habitat model prediction,showed a significant linear relationship with the southern oscillation index,which suggested that El Ni?o episodes eventually provide more preferable habitat for skipjack tuna under ENSO variability.展开更多
Potential fishing zones for skipjack tuna in the Bone Bay-Flores Sea were investigated from satellite-based oceanography and catch data, using a linear model (generalized linear model) constructed from generalized add...Potential fishing zones for skipjack tuna in the Bone Bay-Flores Sea were investigated from satellite-based oceanography and catch data, using a linear model (generalized linear model) constructed from generalized additive models and geographic information systems. Monthly mean remotely sensed sea surface temperature and surface chlorophyll-a concentration during the southeast monsoon (April-August) were used for the year 2012. The best generalized additive model was selected to assess the effect of marine environment variables (sea surface temperature and chlorophyll-a concentration) on skipjack tuna abundance (catch per unit effort). Then, the appropriate linear model was constructed from the functional relationship of the generalized additive model for generating a robust predictive model. Model selection process for the generalized additive model was based on significance of model terms, decrease in residual deviance, and increase in cumulative variance explained, whereas the model selection for the linear model was based on decrease in residual deviance, reduction in Akaike’s Information Criterion, increasing cumulative variance explained and significance of model terms. The best model was selected to predict skipjack tuna abundance and their spatial distribution patterns over entire study area. A simple linear model was used to verify the predicted values. Results indicated that the distribution pattern of potential fishing zones for skipjack during the southeast monsoon were well characterized by sea surface temperatures ranging from 28.5℃ to 30.5 ℃ and chlorophyll-a ranging from 0.10 to 0.20 mg·m-3. Predicted highest catch per unit efforts were significantly consistent with the fishing data (P 2 = 0.8), suggesting that the oceanographic indicators may correspond well with the potential feeding ground for skipjack tuna. This good feeding opportunity for skipjack was driven the dynamics of upwelling operating within study area which are capable of creating a highly potential fishing zone during the southeast monsoon.展开更多
Acid-soluble collagen(ASC) and pepsin-soluble collagen(PSC) from the spine(ASC-SP and PSC-SP) and skull(ASC-SK and PSC-SK) of the skipjack tuna, Katsuwonus pelamis, were successfully isolated and characterized. The yi...Acid-soluble collagen(ASC) and pepsin-soluble collagen(PSC) from the spine(ASC-SP and PSC-SP) and skull(ASC-SK and PSC-SK) of the skipjack tuna, Katsuwonus pelamis, were successfully isolated and characterized. The yields of ASC-SP, PSC-SP, ASC-SK and PSC-SK were(2.47 ± 0.39)%,(5.62 ± 0.82)%,(3.57 ± 0.40)%, and(6.71 ± 0.81)%, respectively, on the basis of dry weight. The four collagens contained Gly(330.2-339.1 residues/1 000 residues) as the major amino acid, and their imino acid contents were between 168.8 and 178.2 residues/1 000 residues. Amino acid composition, SDS-PAGE, and FTIR investigations confirmed that ASC-SP and ASC-SK were mainly composed of type I collagen, and had higher contents of high-molecular weight cross-links than those of PSC-SK and PSC-SP. The FTIR investigation also certified all the collagens had triple helical structure. The denaturation temperatures of ASC-SK, PSC-SK, ASC-SP, and PSC-SP were 17.8, 16.6, 17.6, and 16.5 °C, respectively. All isolated collagens were soluble at acidic pH(1-5) and lost their solubilities when the NaCl concentration was above 2%(W/V). The isolated collagens from the spines and skulls of skipjack tuna could serve as an alternative source of collagens for further application in food, cosmetic, biomedical, and pharmaceutical industries.展开更多
Biological data in fishery ecology have complex structures and are highly heterogeneous.Catch per unit effort(CPUE)estimated from fishery-dependent data are often used to characterize abundance indices(AI)of fish spec...Biological data in fishery ecology have complex structures and are highly heterogeneous.Catch per unit effort(CPUE)estimated from fishery-dependent data are often used to characterize abundance indices(AI)of fish species,which is critical in fish stock assessment.However,additional considerations need to be undertaken to ensure robust estimation because of the latently complicated structures in fishery-dependent data.Here,we elaborated the process of constructing multi-output artificial neural network models to standardize CPUE for heterogeneous fishing operations and applied it to the skipjack tuna(Katsuwonus pelamis)in the western and central Pacific Ocean(WCPO).Seasonal,spatial,and environmental factors were input variables,and the CPUE of four types of skipjack tuna fisheries were set as output variables.The optimal structure for multi-output neural network was evaluated by systematic comparison in 100 runs hold-out cross-validation.The results showed that the final multi-output neural network model with high accuracy can predict the spatial and temporal trends of skipjack tuna abundance.展开更多
For full use of Skipjack tuna(Katsuwonus pelamis)canning by-products,gelatins(STG)were extracted from tuna skins using acid(STG-A),enzyme(STG-E)and hot water(STG-H)methods with yields of 65.30±1.56%,39.83±1....For full use of Skipjack tuna(Katsuwonus pelamis)canning by-products,gelatins(STG)were extracted from tuna skins using acid(STG-A),enzyme(STG-E)and hot water(STG-H)methods with yields of 65.30±1.56%,39.83±1.61%,and 50.97±1.44%,respectively.Due to the high imino acid contents(228,216,and 213 residues/1000 residues for STG-A,STG-E,and STG-H,respectively),STG-A showed the highest transparency and gel strength properties.Amino acid analysis,sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE),ultraviolet(UV)and Fourier transform infrared spectroscopy(FTIR)analysis confirmed that STG-A,STG-E,and STG-H kept the main structure of type I collagen,but enzyme and hot water extraction methods showed much stronger hydrolysis ability onαandβchains.Moreover,neutrase hydrolysate(STG-AH)of STG-A showed the highest 1,1-diphenyl-2-picrylhydrazyl radical(DPPH•)scavenging activity and the fraction STG-AH-І(<3.5 kDa)from STG-AH prepared by ultrafiltration could significantly protect human skin fibroblasts(HSFBs)against ultraviolet-A(UVA)injury.Furthermore,nineteen peptides with high antioxidant activity were purified from STG-AH-Іand identified.These results suggested that STG-AH and its derived antioxidant peptides could serve as potential ingredients applied in health benefiting products for preventing UVA injury.展开更多
Background:Our previous study demonstrated that a dietary intervention with skipjack tuna(Katsuwonus pelamis)elastin peptides alleviated adjuvant-induced arthritis(AIA)and affected the gut microbiota of AIA mice.How-e...Background:Our previous study demonstrated that a dietary intervention with skipjack tuna(Katsuwonus pelamis)elastin peptides alleviated adjuvant-induced arthritis(AIA)and affected the gut microbiota of AIA mice.How-ever,whether the faecal microbiota serves as a surrogate to reflect the dynamics of the gut microbiota has not been determined.Methods:Using 16 S rRNA sequencing technology,the present study investigated the microbiota in three seg-ments(stomach,colon and caecum)of mice to comprehensively define microbial dysregulation and regulation related to rheumatoid arthritis(RA).Results:The results showed that the composition and distribution of the microbiota in the stomach,colon and caecum were significantly different depending on their intrinsic environment and their functions,and the colon possessed the richest and most diverse microbiota structure.The gut microbiota of AIA mice showed ecological dysbiosis,and a dietary intervention with skipjack tuna elastin peptides improved this negative effect,partic-ularly the changes in the gastric microbiota,which were most obvious at RA onset and remission.Implication:The results not only indicate the spatial heterogeneity of the gut microbiota in terms of composition and distribution,but also indicate the potential of the application of elastin peptides for the alleviation of RA.展开更多
为探索流化冰对冰鲜水产品保鲜效果,以鲣鱼鱼肉为研究对象,以传统碎块冰保鲜为对照,探讨流化冰处理对鲣鱼肌肉蛋白质功能特性影响。结果表明:1)流化冰冰粒子呈球形,外表光滑、单位表面积大且流动性能好,8 min内可将鱼肉整体温度由35℃...为探索流化冰对冰鲜水产品保鲜效果,以鲣鱼鱼肉为研究对象,以传统碎块冰保鲜为对照,探讨流化冰处理对鲣鱼肌肉蛋白质功能特性影响。结果表明:1)流化冰冰粒子呈球形,外表光滑、单位表面积大且流动性能好,8 min内可将鱼肉整体温度由35℃降低至1.3℃;2)-4℃贮藏18 d后,流化冰保鲜处理的鱼肉弹性和咀嚼性依次为1.19 mm和5.50 m J,而空白(不加冰)、淡水碎块冰组分别为0.67 mm和1.65 m J、0.95 mm和3.32 m J,可见流化冰对鱼肉质构特性保持效果显著(P<0.05);3)0~18 d贮藏期内,不同处理鲣鱼肌原纤维蛋白含量、Ca2+-ATPase活性及总巯基含量均呈逐渐下降趋势,其中以流化冰处理对鲣鱼蛋白质功能特性的保持效果最佳;此外,流化冰保鲜还兼具有抑制鱼肉氧合肌红蛋白自动氧化、维持肌肉原有色泽的作用。流化冰处理显著保持了鲣鱼肌肉组织的质构和相关蛋白质功能特性,可满足冰鲜水产品远洋、长距离运输和保鲜贮藏要求。展开更多
文摘跳频码序列的性质直接影响着跳频通信的性能 ,计数式跳历 To D( Time of Day)跳频码序列的产生算法是对计数式 To D的分组加密变换。基于这种认识 ,提出了用分组加密 Skipjack算法来构成跳频码序列的产生算法 ,分析和测试表明所得的跳频码序列在均匀性、相关性、复杂性、游程和频隙滞留等方面都能满足跳频码序列的要求 ;在 FPGA上综合的结果说明 ,该算法是一个便于芯片实现的、可实用的跳频码发生器方案。
基金The National Key R&D Program of China under contract Nos 2020YFD0901202 and 2019YFD0901502the National Natural Science Foundation of China under contract Nos 41806110,41506151 and 31902426。
文摘In the western and central Pacific Ocean,upper strata waters exhibit highly dynamic oceanographic features under ENSO variability.This has been proved to be responsible for the dynamic change of both abundance and zonal distribution of skipjack tuna(Katsuwonus pelamis).Although causality has been suggested by researchers using physical-biological interaction models,cumulative evidence needs to be obtained and the tenability of assertion needs to be tested from an ecological habitat perspective,based on fisheries data.For purse seine fishery,the use of catch per unit effort(CPUE)as an indication of the abundance is confusing because of technical improvements over the whole exploitation history and unbalanced individual fishing characteristic of vessels.It is particularly interesting to discriminate between habitat characteristics in comparative scenarios of CPUE application.This study identified habitat traits based on a series of oceanographic factors from a global ocean reanalysis model.A comparison was conducted between two habitat models based on unprocessed purse seine CPUE and standardized CPUE considering fishing characteristics.The results suggest that standardized CPUE could model the regular zonal shift of habitat compatible with the observed fishing efforts transfer,and achieved better prediction capacity than unprocessed CPUE.Furthermore,the habitat of skipjack tuna was also characterized and linked with surface and subsurface thermal environment,ocean current,dissolved oxygen,biotic environment,and ENSO variability.The monthly-averaged habitat suitable index,derived from the optimal habitat model prediction,showed a significant linear relationship with the southern oscillation index,which suggested that El Ni?o episodes eventually provide more preferable habitat for skipjack tuna under ENSO variability.
文摘Potential fishing zones for skipjack tuna in the Bone Bay-Flores Sea were investigated from satellite-based oceanography and catch data, using a linear model (generalized linear model) constructed from generalized additive models and geographic information systems. Monthly mean remotely sensed sea surface temperature and surface chlorophyll-a concentration during the southeast monsoon (April-August) were used for the year 2012. The best generalized additive model was selected to assess the effect of marine environment variables (sea surface temperature and chlorophyll-a concentration) on skipjack tuna abundance (catch per unit effort). Then, the appropriate linear model was constructed from the functional relationship of the generalized additive model for generating a robust predictive model. Model selection process for the generalized additive model was based on significance of model terms, decrease in residual deviance, and increase in cumulative variance explained, whereas the model selection for the linear model was based on decrease in residual deviance, reduction in Akaike’s Information Criterion, increasing cumulative variance explained and significance of model terms. The best model was selected to predict skipjack tuna abundance and their spatial distribution patterns over entire study area. A simple linear model was used to verify the predicted values. Results indicated that the distribution pattern of potential fishing zones for skipjack during the southeast monsoon were well characterized by sea surface temperatures ranging from 28.5℃ to 30.5 ℃ and chlorophyll-a ranging from 0.10 to 0.20 mg·m-3. Predicted highest catch per unit efforts were significantly consistent with the fishing data (P 2 = 0.8), suggesting that the oceanographic indicators may correspond well with the potential feeding ground for skipjack tuna. This good feeding opportunity for skipjack was driven the dynamics of upwelling operating within study area which are capable of creating a highly potential fishing zone during the southeast monsoon.
基金supported by the National Natural Science Foundation of China(No.31001109)the Public Projects of Zhejiang Province(No.2014C33034)the Special Program for the Science and Technology Plan of Zhejiang Province(Nos.2009C03017-2,2011C02003)
文摘Acid-soluble collagen(ASC) and pepsin-soluble collagen(PSC) from the spine(ASC-SP and PSC-SP) and skull(ASC-SK and PSC-SK) of the skipjack tuna, Katsuwonus pelamis, were successfully isolated and characterized. The yields of ASC-SP, PSC-SP, ASC-SK and PSC-SK were(2.47 ± 0.39)%,(5.62 ± 0.82)%,(3.57 ± 0.40)%, and(6.71 ± 0.81)%, respectively, on the basis of dry weight. The four collagens contained Gly(330.2-339.1 residues/1 000 residues) as the major amino acid, and their imino acid contents were between 168.8 and 178.2 residues/1 000 residues. Amino acid composition, SDS-PAGE, and FTIR investigations confirmed that ASC-SP and ASC-SK were mainly composed of type I collagen, and had higher contents of high-molecular weight cross-links than those of PSC-SK and PSC-SP. The FTIR investigation also certified all the collagens had triple helical structure. The denaturation temperatures of ASC-SK, PSC-SK, ASC-SP, and PSC-SP were 17.8, 16.6, 17.6, and 16.5 °C, respectively. All isolated collagens were soluble at acidic pH(1-5) and lost their solubilities when the NaCl concentration was above 2%(W/V). The isolated collagens from the spines and skulls of skipjack tuna could serve as an alternative source of collagens for further application in food, cosmetic, biomedical, and pharmaceutical industries.
基金supported by the National Key R&D Program of China(No.2023YFD2401303).
文摘Biological data in fishery ecology have complex structures and are highly heterogeneous.Catch per unit effort(CPUE)estimated from fishery-dependent data are often used to characterize abundance indices(AI)of fish species,which is critical in fish stock assessment.However,additional considerations need to be undertaken to ensure robust estimation because of the latently complicated structures in fishery-dependent data.Here,we elaborated the process of constructing multi-output artificial neural network models to standardize CPUE for heterogeneous fishing operations and applied it to the skipjack tuna(Katsuwonus pelamis)in the western and central Pacific Ocean(WCPO).Seasonal,spatial,and environmental factors were input variables,and the CPUE of four types of skipjack tuna fisheries were set as output variables.The optimal structure for multi-output neural network was evaluated by systematic comparison in 100 runs hold-out cross-validation.The results showed that the final multi-output neural network model with high accuracy can predict the spatial and temporal trends of skipjack tuna abundance.
基金funded by the National Natural Science Foundation of China(No.82073764)the Ten-thousand Talents Plan of Zhejiang Province(No.2019R52026).
文摘For full use of Skipjack tuna(Katsuwonus pelamis)canning by-products,gelatins(STG)were extracted from tuna skins using acid(STG-A),enzyme(STG-E)and hot water(STG-H)methods with yields of 65.30±1.56%,39.83±1.61%,and 50.97±1.44%,respectively.Due to the high imino acid contents(228,216,and 213 residues/1000 residues for STG-A,STG-E,and STG-H,respectively),STG-A showed the highest transparency and gel strength properties.Amino acid analysis,sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE),ultraviolet(UV)and Fourier transform infrared spectroscopy(FTIR)analysis confirmed that STG-A,STG-E,and STG-H kept the main structure of type I collagen,but enzyme and hot water extraction methods showed much stronger hydrolysis ability onαandβchains.Moreover,neutrase hydrolysate(STG-AH)of STG-A showed the highest 1,1-diphenyl-2-picrylhydrazyl radical(DPPH•)scavenging activity and the fraction STG-AH-І(<3.5 kDa)from STG-AH prepared by ultrafiltration could significantly protect human skin fibroblasts(HSFBs)against ultraviolet-A(UVA)injury.Furthermore,nineteen peptides with high antioxidant activity were purified from STG-AH-Іand identified.These results suggested that STG-AH and its derived antioxidant peptides could serve as potential ingredients applied in health benefiting products for preventing UVA injury.
基金sponsored by the National Key R&D Program of China(2018YFD0901102)the Fund of State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products(ZS20190105)+2 种基金the Natural Foundation of Zhejiang Province(LQ22D060002)the Cooperative Project of Vigour Health Products Co.,Ltd,Shenzhenthe K.C.Wong Magna Fund of Ningbo University.
文摘Background:Our previous study demonstrated that a dietary intervention with skipjack tuna(Katsuwonus pelamis)elastin peptides alleviated adjuvant-induced arthritis(AIA)and affected the gut microbiota of AIA mice.How-ever,whether the faecal microbiota serves as a surrogate to reflect the dynamics of the gut microbiota has not been determined.Methods:Using 16 S rRNA sequencing technology,the present study investigated the microbiota in three seg-ments(stomach,colon and caecum)of mice to comprehensively define microbial dysregulation and regulation related to rheumatoid arthritis(RA).Results:The results showed that the composition and distribution of the microbiota in the stomach,colon and caecum were significantly different depending on their intrinsic environment and their functions,and the colon possessed the richest and most diverse microbiota structure.The gut microbiota of AIA mice showed ecological dysbiosis,and a dietary intervention with skipjack tuna elastin peptides improved this negative effect,partic-ularly the changes in the gastric microbiota,which were most obvious at RA onset and remission.Implication:The results not only indicate the spatial heterogeneity of the gut microbiota in terms of composition and distribution,but also indicate the potential of the application of elastin peptides for the alleviation of RA.
文摘为探索流化冰对冰鲜水产品保鲜效果,以鲣鱼鱼肉为研究对象,以传统碎块冰保鲜为对照,探讨流化冰处理对鲣鱼肌肉蛋白质功能特性影响。结果表明:1)流化冰冰粒子呈球形,外表光滑、单位表面积大且流动性能好,8 min内可将鱼肉整体温度由35℃降低至1.3℃;2)-4℃贮藏18 d后,流化冰保鲜处理的鱼肉弹性和咀嚼性依次为1.19 mm和5.50 m J,而空白(不加冰)、淡水碎块冰组分别为0.67 mm和1.65 m J、0.95 mm和3.32 m J,可见流化冰对鱼肉质构特性保持效果显著(P<0.05);3)0~18 d贮藏期内,不同处理鲣鱼肌原纤维蛋白含量、Ca2+-ATPase活性及总巯基含量均呈逐渐下降趋势,其中以流化冰处理对鲣鱼蛋白质功能特性的保持效果最佳;此外,流化冰保鲜还兼具有抑制鱼肉氧合肌红蛋白自动氧化、维持肌肉原有色泽的作用。流化冰处理显著保持了鲣鱼肌肉组织的质构和相关蛋白质功能特性,可满足冰鲜水产品远洋、长距离运输和保鲜贮藏要求。
