It has been reported that diallyl sulfide (DAS), a sulfur-containing volatile compound in garlic (Allium sativum ), exerts anticarcinogenic activity in various rodent tumor models. In the present study, the antitumor ...It has been reported that diallyl sulfide (DAS), a sulfur-containing volatile compound in garlic (Allium sativum ), exerts anticarcinogenic activity in various rodent tumor models. In the present study, the antitumor property of DAS was tested in Swiss albino mice in the two steqe initiation-promotion mouse skin carcinogenesis. Skin cancers were initiated topically with a single subcarcinOgenic dose (52μg) of 7, 12-dimethyl benz (a) anthracene (DMBA). Promotion was performed by twice weekly applications of 12-O-tetradecanoyl phorbol-13-acetate (TPA) at a dose of 5μg/animal for 32 weeks. DAS was applied topically (250μg/animal) thrice weekly for 3 weeks for anti-initiating and 1 h prior to each promotion treatment for anti-promoting studies. The results showed that the treatment schedule of DAS can effectively delay the onset of tumorigenesis and reduce the cumulative number of tumors and the average number of tumors per mouse. In groups in which DAS applied prior to initiation or promotion, a significant population of the aniinals remained tumor-free till the termination of experiment. These findings suggest that DAS can effectively inhibit chemically induced mouse skincarcinogenesis.展开更多
In the present investigations, the antitumorigenic effect of black tea polyphenols (BTP) in twcrstage mouse skin model of carcinogenesis was studied. The animals were initiated with a single 'subcarcinogenic' ...In the present investigations, the antitumorigenic effect of black tea polyphenols (BTP) in twcrstage mouse skin model of carcinogenesis was studied. The animals were initiated with a single 'subcarcinogenic' topical dose (52 μg/200 μl acetone ) of 7, 12-dimethylbenzanthracene (DMBA). To evaluate the anti-tumour initiating activity, BTP was topically applied twice a week for three weeks prior to DMBA application, followed by topical treatment with 12-o-tetradecanoyl phorbol-13-acetate (TPA) (5 μg/200 μl acetone, 2x/wk. ) as promoter. For evaluation of antitumor promoting activity, BTP was applied prior to each treatment of TPA. BTP application showed marked inhibitory effect as antitumour initiator as well as antitumour promoter in mouse skin medel of two-stage carcinogenesis. Since initiation involves genetic pathway and tumour promotion involves epigenetic pathway, it seems that BTP exerts its antitumorigenic effect by altering both genetic and epigenetic pathways展开更多
Skin is the largest organ in human body,and it plays an important role in regulating physiological microenvironments and acts as a barrier to protect human body from harmful intrusions.The demand for fully functional ...Skin is the largest organ in human body,and it plays an important role in regulating physiological microenvironments and acts as a barrier to protect human body from harmful intrusions.The demand for fully functional skin models(also called skin equivalents,SE)in an in-vivo mimicking culturing microenvironment has been increased dramatically due to the fast development in skin disease treatments and skin care products.Owing to the emerging of the concept and technology of organ-on-chips along with the three-dimensional(3D)bioprinting technology,3D skin models and their applications have been fast evolving.In this paper,the advances in the development of 3D skin models along with skin-on-a-chip(SOC)are reviewed and commented.One of the findings with this paper is that the SOC together with the 3D bioprinting technology is promising to construct fully functional 3D skin models in the field of pharmaceutical and cosmetic industries.展开更多
Keratinocytes and fibroblasts,derived from hiPSCs,were used to construct the human epidermal model by a culture patch made by monolayer poly-(lactic-co-glycolic acid)(PLGA)nanofibers and a human skin-on-a-chip device....Keratinocytes and fibroblasts,derived from hiPSCs,were used to construct the human epidermal model by a culture patch made by monolayer poly-(lactic-co-glycolic acid)(PLGA)nanofibers and a human skin-on-a-chip device.Unlike the conventional culture dish method,two different epidermal cells are successfully adhered to the front and back sides of the patch,which produces a three-dimensional nanofibrous scaffold similar to a natural extracellular matrix before the patch was cultured in the skin-on-a-chip device to mimic the physiological conditions of human skin.As expected,the differentiated hiPSCs show the expression of keratinocyte-and fibroblast-specific proteins on the patch,and the layering is found between these two kinds of cells,indicating that this approach creates a powerful in vitro system for modeling skin development and diseases.展开更多
A recombinant skin model is a model in which skin cells are grown in vitro on a bioactive scaffold and provided with adequate nutrition to promote cell proliferation and differentiation to produce a mock skin structur...A recombinant skin model is a model in which skin cells are grown in vitro on a bioactive scaffold and provided with adequate nutrition to promote cell proliferation and differentiation to produce a mock skin structure and biological features. The development of recombinant skin models allows for effective and scientifically sound in vitro evaluation tests. This review briefly summarizes the overview of recombinant skin models and the progress of their application in in vitro evaluation which focuses on three aspects: skin irritation, skin corrosivity, and anti-skin aging. Moreover, an outlook on the future development of recombinant skin models is also provided in this review.展开更多
In this work, the suitability of lipid stripping as an alternative model of stratum corneum for plasma medical studies was investigated. Plasma treatment experiments were performed on samples prepared by the cyanoacry...In this work, the suitability of lipid stripping as an alternative model of stratum corneum for plasma medical studies was investigated. Plasma treatment experiments were performed on samples prepared by the cyanoacrylat stripping method. Therefore, two different dielectric barrier discharge-based plasma sources driven by high-voltage pulses in the microsecond and nanosecond range were applied. The lipid sample heating, change in pH-value, and the interaction with plasma-induced UV-radiation are presented and discussed with respect to existing findings on skin samples. After the plasma treatment, the lipid stripping shows similar changes compared to human skin relating to sample heating and pH-value. The investigation of the interplay with UV- radiation shows a high absorption in the wavelength range of 250 nm up to 400 nm. Further, the thickness, surface structure, and composition of lipid stripping samples were determined. The stripped sample shows a thickness of 3 ± 1 μm whereby approximately 30% of the sample surface is covered by lipids. In addition, it was shown that there are no changes in structure caused by the sample preparation. Based on the results of this work, it can be stated that lipid stripping represents an appropriate skin model for plasma medical investigations.展开更多
Via the use of Mimedisc~? as a scaffold, the normal human dermal fibroblasts were first seeding into the scaffold to form a dermal equivalent after three weeks, and then seeding normal human epidermal keratinocytes on...Via the use of Mimedisc~? as a scaffold, the normal human dermal fibroblasts were first seeding into the scaffold to form a dermal equivalent after three weeks, and then seeding normal human epidermal keratinocytes on it, followed by immersion and air-liquid interphase culture stage, to finally form a 3 D sponge matrix skin model which has a very similar structure of normal human skin(NHS). The results of Masson staining, and immunohistochemistry(IHC), immunofluorescence(IF) staininccg show that this skin model has a very similar structure and key protein expression of NHS. Meanwhile, this model was used as a tool to investigate the effect of Himalaya cedar extract on Dermal-Epidermal Junction(DEJ) in vitro. Results show that the Himalayan cedar extract can significantly increase the thickness of the epidermis, thus increase the barrier capacity of the skin to resist external environment. Further, this Himalaya cedar extract can promote the expressions of Laminin 5 and Collagen VII of DEJ. The expressions of the Laminin 5 and Collagen VII increased 20% and 47% respectively.So the Himalaya cedar extract displays the potential to be applied in development of anti-aging cosmetics.展开更多
Transmission of light through model human epidermal samples is investigated at four different wavelengths and at varying ambient humidity. Light from light emitting diodes (LEDs) is used for transmission measurements ...Transmission of light through model human epidermal samples is investigated at four different wavelengths and at varying ambient humidity. Light from light emitting diodes (LEDs) is used for transmission measurements through the samples at a UVA wavelength of 365 nm, and visible wavelengths of 460 nm, 500 nm, and 595 nm. Ambient air-humidity is varied between 20% and 100%. Results show that for high ambient humidity, near 100%, transmission of light through the epidermis is higher than at low ambient humidity, 60% or lower. These results are explained with a simple model of epidermis as a turbid medium and the effect of adsorbed water in reducing light-scattering by refractive-index-matching. Biological implications of increased light-transmission through epidermis at high ambient humidity are discussed.展开更多
This comprehensive review explores the multifaceted landscape of skin bioprinting,revolutionizing dermatological research.The applications of skin bioprinting utilizing techniques like extrusion-,droplet-,laser-and li...This comprehensive review explores the multifaceted landscape of skin bioprinting,revolutionizing dermatological research.The applications of skin bioprinting utilizing techniques like extrusion-,droplet-,laser-and light-based methods,with specialized bioinks for skin biofabrication have been critically reviewed along with the intricate aspects of bioprinting hair follicles,sweat glands,and achieving skin pigmentation.Challenges remain with the need for vascularization,safety concerns,and the integration of automated processes for effective clinical translation.The review further investigates the incorporation of biosensor technologies,emphasizing their role in monitoring and enhancing the wound healing process.While highlighting the remarkable progress in the field,critical limitations and concerns are critically examined to provide a balanced perspective.This synthesis aims to guide scientists,engineers,and healthcare providers,fostering a deeper understanding of the current state,challenges,and future directions in skin bioprinting for transformative applications in tissue engineering and regenerative medicine.展开更多
Background:Black ginseng is a unique processed product of ginseng,a source of health food,medicine and modern cosmetics.Purpose:The purpose of this study was to develop a new application for preventing inflammatory ag...Background:Black ginseng is a unique processed product of ginseng,a source of health food,medicine and modern cosmetics.Purpose:The purpose of this study was to develop a new application for preventing inflammatory aging.Method:To investigate the effects and mechanisms of action of black ginseng extract(BGE)on inflammatory aging,models of inflammatory aging were established in human foreskin fibroblasts cells and reconstructed 3D skin model.Result:The results showed that MMP-1 expression in human foreskin fibroblasts was reduced at a concentration of 4μg/mL BGE.At a concentration of 0.1%,BGE not only inhibited the secretion of PGE 2 in reconstructed 3D skin model damaged by SDS but also promoted the expression of TIMP-1.Furthermore,the composition of three rare ginsenosides in the BGE and their binding affinity to target proteins associated with inflammatory aging were analyzed using high-performance liquid chromatography(HPLC)and molecular docking technique.Conclusion:BGE has potential application value in alleviating skin inflammatory aging.展开更多
Polydeoxyribonucleotide(PDRN)has been utilized in clinical practice for an extended period;however,its application in the cosmetics is relatively recent.This study aims to explore the research progress of PDRN regardi...Polydeoxyribonucleotide(PDRN)has been utilized in clinical practice for an extended period;however,its application in the cosmetics is relatively recent.This study aims to explore the research progress of PDRN regarding its skin repair and anti-aging properties.To evaluate the potential skin repair,soothing,anti-wrinkle and firming effects of PDRN essence via both in vitro and in vivo test method.This study employed in vitro and in vivo test to assess the skin repair and anti-wrinkle effects.The 3D skin model(FulKutis®)was utilized to verify the effects of the PDRN essence on anti-inflammation,barrier repair,and anti-aging.In human test,a randomized,half-face comparison test with PDRN essence and the placebo was conducted with 33 Chinese subjects who met the test criteria.During the test,the skin conditions in terms of the a*value,facial red area ratio,and Transepidermal Water Loss(TEWL)were evaluated at 30 min,4 h,1 day,and 3 days;the skin conditions in terms of skin elasticity and firmness,skin wrinkles were evaluated at 42 days.Subjects’self-assessments were collected at 3 days and 42 days.The FulKutis■experiments demonstrated that the PDRN essence significantly decreased the levels of IL-1α,TNF-α,and PGE2,and improved the protein levels of Filaggrin,Loricrin,Collagen I,Collagen IV,and the thickness of the epidermal living cell layer.In the human study,the a*value,red area ratio,TEWL,and skin-related parameters(R2,R5,R7,F4,and skin wrinkles)on the test face side exhibited greater improvement compared to the control face side with statistically significant differences.Additionally,subject selfassessments revealed high satisfaction with the PDRN essence.The PDRN essence applied after non-ablative fractional laser treatment promotes barrier repair and soothing effects,shortens the recovery period,and enhances facial firmness and wrinkle improvement at the crow’s feet and undereye regions.No adverse events related to the tested cosmetics were observed.展开更多
By compounding the cranberry extract,lavender flower extract,Kakadu plum extract,edelweiss flower/leaf extract and aloe vera extract into a composite plant extract(BIRC-Rosée WT100),the whitening effects of this ...By compounding the cranberry extract,lavender flower extract,Kakadu plum extract,edelweiss flower/leaf extract and aloe vera extract into a composite plant extract(BIRC-Rosée WT100),the whitening effects of this composite plant extract in antioxidation,anti-inflammation,skin barrier maintenance,and inhibition of melanin production and transport were evaluated.By comparing the inhibitory effects on tyrosinase activity between the composite plant extract andα-arbutin,and then analyzing the decline of apparent chromaticity,apparent brightness and melanin content through 3D melanin skin model platform,the comprehensive whitening effect was evaluated and compared with that of kojic acid.The results showed that,the composite plant extract(BIRC-Rosée WT100)had a tyrosinase inhibition rate of 98.99%at the original concentration,which was much higher than that ofα-arbutin.In 3D melanin skin model,the apparent chromaticity and brightness could be effectively improved,and moreover,the total amount of melanin in the skin could be reduced,showing remarkable whitening effect.展开更多
One being developed automatic sweep robot, need to estimate if anyone is on a certain range of road ahead then automatically adjust running speed, in order to ensure work efficiency and operation safety. This paper pr...One being developed automatic sweep robot, need to estimate if anyone is on a certain range of road ahead then automatically adjust running speed, in order to ensure work efficiency and operation safety. This paper proposed a method using face detection to predict the data of image sensor. The experimental results show that, the proposed algorithm is practical and reliable, and good outcome have been achieved in the application of instruction robot.展开更多
Objectives: Exposing skin to moderate ionic osmotic stress (MIOS) triggers several biochemical responses. The objective of this work is to reveal the mechanism triggered by MIOS on the skin surface. Furthermore, this ...Objectives: Exposing skin to moderate ionic osmotic stress (MIOS) triggers several biochemical responses. The objective of this work is to reveal the mechanism triggered by MIOS on the skin surface. Furthermore, this work aims to study the involvement of the Nrf2 (nuclear factor erythroid-2-related factor 2) pathway, activated by MIOS, and its beneficial effect in protecting skin against stress via the stimulation of phase II enzymes. Methods: HaCaT cells and human skin organ culture were exposed to Dead Sea Water (DSW) as MIOS inducers and the induction of internal ROS elevation, Nrf2 translocation, mRNA gene expressions of the phase II enzymes, heme-oxygenase 1 (HO1), and Catalase (CAT) were determined. Results: Skin exposure to MIOS increases Nrf2 translocation to the nucleus, leading to increased levels of ROS, HO1, and CAT. Furthermore, exposing skin to MIOS promotes protection against UVB-related risks. This is demonstrated by attenuation of the expression of biomarkers, related to UVB-induced damage, Caspase-3, IL-8, and IL-1β. Conclusions: Skin exposure to MIOS leads to the activation of Nrf2 skin defense pathway and, therefore, could present beneficial advantages to human skin health, as demonstrated on human skin models. The beneficial effects of MIOS, induced by DSW are significantly superior to eq. NaCl brine, suggests that MIOS protection of skin against stress is partially related to specific mineral combinations.展开更多
The development of bionic skin is crucial for progressing our understanding of skin physiology and pathologies,while the precise replication of human skin features in skin models remains a challenge.In this study,we p...The development of bionic skin is crucial for progressing our understanding of skin physiology and pathologies,while the precise replication of human skin features in skin models remains a challenge.In this study,we present a biomimetic,gender-specific skin model utilizing a gonads/epidermis-on-a-chip platform.This innovative approach seamlessly integrates ex vivo cultured human epidermal tissue and gonad cell aggregates into a microfluidic system,facilitating the exploration of the regulatory effects of sex hormones on the epidermis.Based on this platform,we have found that estradiol can enhance proliferation and inhibit apoptosis of keratinocytes.Conversely,testosterone notably induces keratinocyte differentiation,leading to epidermal hyperkeratosis.These observations align with the well-documented phenomenon that males possess thicker and more durable skin,while females’skin exhibits superior repair capabilities.Thus,we believe that our biomimetic gender-specific skin model can offer a promising avenue for obtaining higher-certainty evidence of human skin-related conditions and find important applications in the areas of cosmetics and biomedicine.展开更多
Realistic human skin rendering has been a long-standing challenge in computer graphics.Recently,biophysical-based skin rendering has received increasing attention,as it provides a more realistic skin-rendering and a m...Realistic human skin rendering has been a long-standing challenge in computer graphics.Recently,biophysical-based skin rendering has received increasing attention,as it provides a more realistic skin-rendering and a more intuitive way to adjust the skin style.In this work,we present a novel heterogeneous biophysical-based volume rendering method for human skin that improves the realism of skin appearance while easily simulating various types of skin effects,including skin diseases,by modifying biological coefficient textures.Specifically,we introduce a two-layer skin representation by mesh deformation that explicitly models the epidermis and dermis with heterogeneous volumetric medium layers containing the corresponding spatially varying melanin and hemoglobin,respectively.Furthermore,to better facilitate skin acquisition,we introduced a learning-based framework that automatically estimates spatially varying biological coefficients from an albedo texture,enabling biophysical-based and intuitive editing,such as tanning,pathological vitiligo,and freckles.We illustrated the effects of multiple skinediting applications and demonstrated superior quality to the commonly used random walk skin-rendering method,with more convincing skin details regarding subsurface scattering.展开更多
This paper presents a new face detection approach to real-time applications, which is based on the skin color model and the morphological filtering. First the non-skin color pixels of the input image are removed based...This paper presents a new face detection approach to real-time applications, which is based on the skin color model and the morphological filtering. First the non-skin color pixels of the input image are removed based on the skin color model in the YC rC b chrominance space, from which we extract candidate human face regions. Then a mathematical morphological filter is used to remove noisy regions and fill the holes in the candidate skin color regions. We adopt the similarity between the human face features and the candidate face regions to locate the face regions in the original image. We have implemented the algorithm in our smart media system. The experiment results show that this system is effective in real-time applications.展开更多
Although multi-view monitoring techniques have been widely applied in skinned model reconstruction and movement analysis,traditional systems using high-performance Personal Computers(PCs),or industrial cameras are oft...Although multi-view monitoring techniques have been widely applied in skinned model reconstruction and movement analysis,traditional systems using high-performance Personal Computers(PCs),or industrial cameras are often prohibitive due to high costs and limited scalability.Here,we introduce an affordable,scalable multi-view image acquisition system for skinned model reconstruction in animal studies,utilizing consumer Android devices and a wireless network for synchronized monitoring named Rodent Arena Multi-View Monitor(RAMM).It uses smartphones as camera nodes with local data storage,enabling cost-effective scalability.Its custom synchronization solution and portability make it ideal for research and education in rodent behavior analysis,offering a practical alternative for institutions with limited budgets.Furthermore,the portability and flexibility of this system make it an ideal tool for rodent skinned model research based on multi-view image acquisition.To evaluate the performance,we perform an oscilloscope analysis to ensure effectiveness of synchronization.A 45-camera node setup is built to highlight RAMM’s cost efficiency and ease in constructing large-scale systems.Additionally,the data quality is validated using the Instant Neural Graphics Primitives(Instant-NGP)method.Remarkable results were achieved with a 30.49 dB PSNR by utilizing only 25 images with intrinsic and extrinsic parameters,fulfilling the requirements for well-synchronized data used in 3D representation algorithms.展开更多
Skin,as the outmost layer of human body,is frequently exposed to environmental stressors including pollutants and ultraviolet(UV),which could lead to skin disorders.Generally,skin response process to ultraviolet B(UVB...Skin,as the outmost layer of human body,is frequently exposed to environmental stressors including pollutants and ultraviolet(UV),which could lead to skin disorders.Generally,skin response process to ultraviolet B(UVB)irradiation is a nonlinear dynamic process,with unknown underlying molecular mechanism of critical transition.Here,the landscape dynamic network biomarker(lDNB)analysis of time series transcriptome data on 3D skin model was conducted to reveal the complicated process of skin response to UV irradiation at both molecular and network levels.The advanced l-DNB analysis approach showed that:(i)there was a tipping point before critical transition state during pigmentation process,validated by 3D skin model;(ii)13 core DNB genes were identified to detect the tipping point as a network biomarker,supported by computational assessment;(iii)core DNB genes such as COL7A1 and CTNNB1 can effectively predict skin lightening,validated by independent human skin data.Overall,this study provides new insights for skin response to repetitive UVB irradiation,including dynamic pathway pattern,biphasic response,and DNBs for skin lightening change,and enables us to further understand the skin resilience process after external stress.展开更多
Laser lipolysis can effectively treat obesity and its associated diseases, such as hypertension, fatty liver, and hyperlipidemia. However, currently available invasive laser lipolysis, which transmits laser to a fiber...Laser lipolysis can effectively treat obesity and its associated diseases, such as hypertension, fatty liver, and hyperlipidemia. However, currently available invasive laser lipolysis, which transmits laser to a fiber-optic catheter inserted into the subcutaneous tissue for irradiation through an incision, may cause hematomas, infections, and empyrosis. The current study presents a novel, noninvasive approach for laser lipolysis, which directly irradiates the intact skin surface without an incision and preferentially targets adipose tissue at the near-infrared band. High laser energy is necessary to damage adipocytes; however, this may carbonate and burn the dermis. Therefore, the introduction of skin cooling is essential to avoid unwanted hyperthermal injury and improve the threshold of radiant exposure. In the current study, we investigated a novel noninvasive approach assisted with skin cooling by establishing a homogeneous multi-layer skin model. In this method, light propagation in the skin was simulated by using the Monte Carlo method. Skin cooling was employed before laser irradiation to protect the epidermis from thermal damage, which was treated as a boundary condition based on Newton's law. The numerical results showed that the photons were deposited in the adipose layer more than in the other layers. Laser can effectively destroy adipose tissue at an energy density of >200 J/cm^2 at 1210 nm wavelength, whereas at least 300 J/cm^2 is required at 1064 nm to achieve the same effect. In this experiment, at >5 s pulse width, the selectivity of adipose was not obvious. Moreover, the results indicated that 60 ms R134a or R404a spray can effectively reduce the temperature of the epidermis. R404a exhibited a stronger cooling effect than R134a. Cold air cooling at -10 °C for 10 s could effectively decrease the skin temperature, and deeper cooling could be achieved by cold air cooling compared with cryogen spray cooling.展开更多
文摘It has been reported that diallyl sulfide (DAS), a sulfur-containing volatile compound in garlic (Allium sativum ), exerts anticarcinogenic activity in various rodent tumor models. In the present study, the antitumor property of DAS was tested in Swiss albino mice in the two steqe initiation-promotion mouse skin carcinogenesis. Skin cancers were initiated topically with a single subcarcinOgenic dose (52μg) of 7, 12-dimethyl benz (a) anthracene (DMBA). Promotion was performed by twice weekly applications of 12-O-tetradecanoyl phorbol-13-acetate (TPA) at a dose of 5μg/animal for 32 weeks. DAS was applied topically (250μg/animal) thrice weekly for 3 weeks for anti-initiating and 1 h prior to each promotion treatment for anti-promoting studies. The results showed that the treatment schedule of DAS can effectively delay the onset of tumorigenesis and reduce the cumulative number of tumors and the average number of tumors per mouse. In groups in which DAS applied prior to initiation or promotion, a significant population of the aniinals remained tumor-free till the termination of experiment. These findings suggest that DAS can effectively inhibit chemically induced mouse skincarcinogenesis.
文摘In the present investigations, the antitumorigenic effect of black tea polyphenols (BTP) in twcrstage mouse skin model of carcinogenesis was studied. The animals were initiated with a single 'subcarcinogenic' topical dose (52 μg/200 μl acetone ) of 7, 12-dimethylbenzanthracene (DMBA). To evaluate the anti-tumour initiating activity, BTP was topically applied twice a week for three weeks prior to DMBA application, followed by topical treatment with 12-o-tetradecanoyl phorbol-13-acetate (TPA) (5 μg/200 μl acetone, 2x/wk. ) as promoter. For evaluation of antitumor promoting activity, BTP was applied prior to each treatment of TPA. BTP application showed marked inhibitory effect as antitumour initiator as well as antitumour promoter in mouse skin medel of two-stage carcinogenesis. Since initiation involves genetic pathway and tumour promotion involves epigenetic pathway, it seems that BTP exerts its antitumorigenic effect by altering both genetic and epigenetic pathways
文摘Skin is the largest organ in human body,and it plays an important role in regulating physiological microenvironments and acts as a barrier to protect human body from harmful intrusions.The demand for fully functional skin models(also called skin equivalents,SE)in an in-vivo mimicking culturing microenvironment has been increased dramatically due to the fast development in skin disease treatments and skin care products.Owing to the emerging of the concept and technology of organ-on-chips along with the three-dimensional(3D)bioprinting technology,3D skin models and their applications have been fast evolving.In this paper,the advances in the development of 3D skin models along with skin-on-a-chip(SOC)are reviewed and commented.One of the findings with this paper is that the SOC together with the 3D bioprinting technology is promising to construct fully functional 3D skin models in the field of pharmaceutical and cosmetic industries.
基金by the National Natural Science Foundation of China(Nos.31600801 and 11804121)the Natural Science Foundation of Hubei(No.2019CFB558)the Central Government Guides the Special Fund Project of Local Scientific and Technological Development(No.[2016]109)。
文摘Keratinocytes and fibroblasts,derived from hiPSCs,were used to construct the human epidermal model by a culture patch made by monolayer poly-(lactic-co-glycolic acid)(PLGA)nanofibers and a human skin-on-a-chip device.Unlike the conventional culture dish method,two different epidermal cells are successfully adhered to the front and back sides of the patch,which produces a three-dimensional nanofibrous scaffold similar to a natural extracellular matrix before the patch was cultured in the skin-on-a-chip device to mimic the physiological conditions of human skin.As expected,the differentiated hiPSCs show the expression of keratinocyte-and fibroblast-specific proteins on the patch,and the layering is found between these two kinds of cells,indicating that this approach creates a powerful in vitro system for modeling skin development and diseases.
文摘A recombinant skin model is a model in which skin cells are grown in vitro on a bioactive scaffold and provided with adequate nutrition to promote cell proliferation and differentiation to produce a mock skin structure and biological features. The development of recombinant skin models allows for effective and scientifically sound in vitro evaluation tests. This review briefly summarizes the overview of recombinant skin models and the progress of their application in in vitro evaluation which focuses on three aspects: skin irritation, skin corrosivity, and anti-skin aging. Moreover, an outlook on the future development of recombinant skin models is also provided in this review.
基金supported by the European Regional Development Funds(EFRE)and the Workgroup Innovative Projects of Lower Saxony(AGiP)in the frame of the Lower Saxony Innovation Network for Plasma Technology(NIP),project funding reference number W2-80029388.
文摘In this work, the suitability of lipid stripping as an alternative model of stratum corneum for plasma medical studies was investigated. Plasma treatment experiments were performed on samples prepared by the cyanoacrylat stripping method. Therefore, two different dielectric barrier discharge-based plasma sources driven by high-voltage pulses in the microsecond and nanosecond range were applied. The lipid sample heating, change in pH-value, and the interaction with plasma-induced UV-radiation are presented and discussed with respect to existing findings on skin samples. After the plasma treatment, the lipid stripping shows similar changes compared to human skin relating to sample heating and pH-value. The investigation of the interplay with UV- radiation shows a high absorption in the wavelength range of 250 nm up to 400 nm. Further, the thickness, surface structure, and composition of lipid stripping samples were determined. The stripped sample shows a thickness of 3 ± 1 μm whereby approximately 30% of the sample surface is covered by lipids. In addition, it was shown that there are no changes in structure caused by the sample preparation. Based on the results of this work, it can be stated that lipid stripping represents an appropriate skin model for plasma medical investigations.
文摘Via the use of Mimedisc~? as a scaffold, the normal human dermal fibroblasts were first seeding into the scaffold to form a dermal equivalent after three weeks, and then seeding normal human epidermal keratinocytes on it, followed by immersion and air-liquid interphase culture stage, to finally form a 3 D sponge matrix skin model which has a very similar structure of normal human skin(NHS). The results of Masson staining, and immunohistochemistry(IHC), immunofluorescence(IF) staininccg show that this skin model has a very similar structure and key protein expression of NHS. Meanwhile, this model was used as a tool to investigate the effect of Himalaya cedar extract on Dermal-Epidermal Junction(DEJ) in vitro. Results show that the Himalayan cedar extract can significantly increase the thickness of the epidermis, thus increase the barrier capacity of the skin to resist external environment. Further, this Himalaya cedar extract can promote the expressions of Laminin 5 and Collagen VII of DEJ. The expressions of the Laminin 5 and Collagen VII increased 20% and 47% respectively.So the Himalaya cedar extract displays the potential to be applied in development of anti-aging cosmetics.
文摘Transmission of light through model human epidermal samples is investigated at four different wavelengths and at varying ambient humidity. Light from light emitting diodes (LEDs) is used for transmission measurements through the samples at a UVA wavelength of 365 nm, and visible wavelengths of 460 nm, 500 nm, and 595 nm. Ambient air-humidity is varied between 20% and 100%. Results show that for high ambient humidity, near 100%, transmission of light through the epidermis is higher than at low ambient humidity, 60% or lower. These results are explained with a simple model of epidermis as a turbid medium and the effect of adsorbed water in reducing light-scattering by refractive-index-matching. Biological implications of increased light-transmission through epidermis at high ambient humidity are discussed.
基金supported by National Institutes of Health Award(Nos.R01DE028614(I T O)and R21AR082668(I T O)),and 2236 CoCirculation2 of TUBITAK award(No.121C359(I T O))supported by The Assistant Secretary of Defense for Health Affairs endorsed by the Department of Defense,in the amount of($1986275)through the Peer Reviewed Medical Research Program under Award Number(No.HT9425-23-1-0487)。
文摘This comprehensive review explores the multifaceted landscape of skin bioprinting,revolutionizing dermatological research.The applications of skin bioprinting utilizing techniques like extrusion-,droplet-,laser-and light-based methods,with specialized bioinks for skin biofabrication have been critically reviewed along with the intricate aspects of bioprinting hair follicles,sweat glands,and achieving skin pigmentation.Challenges remain with the need for vascularization,safety concerns,and the integration of automated processes for effective clinical translation.The review further investigates the incorporation of biosensor technologies,emphasizing their role in monitoring and enhancing the wound healing process.While highlighting the remarkable progress in the field,critical limitations and concerns are critically examined to provide a balanced perspective.This synthesis aims to guide scientists,engineers,and healthcare providers,fostering a deeper understanding of the current state,challenges,and future directions in skin bioprinting for transformative applications in tissue engineering and regenerative medicine.
文摘Background:Black ginseng is a unique processed product of ginseng,a source of health food,medicine and modern cosmetics.Purpose:The purpose of this study was to develop a new application for preventing inflammatory aging.Method:To investigate the effects and mechanisms of action of black ginseng extract(BGE)on inflammatory aging,models of inflammatory aging were established in human foreskin fibroblasts cells and reconstructed 3D skin model.Result:The results showed that MMP-1 expression in human foreskin fibroblasts was reduced at a concentration of 4μg/mL BGE.At a concentration of 0.1%,BGE not only inhibited the secretion of PGE 2 in reconstructed 3D skin model damaged by SDS but also promoted the expression of TIMP-1.Furthermore,the composition of three rare ginsenosides in the BGE and their binding affinity to target proteins associated with inflammatory aging were analyzed using high-performance liquid chromatography(HPLC)and molecular docking technique.Conclusion:BGE has potential application value in alleviating skin inflammatory aging.
文摘Polydeoxyribonucleotide(PDRN)has been utilized in clinical practice for an extended period;however,its application in the cosmetics is relatively recent.This study aims to explore the research progress of PDRN regarding its skin repair and anti-aging properties.To evaluate the potential skin repair,soothing,anti-wrinkle and firming effects of PDRN essence via both in vitro and in vivo test method.This study employed in vitro and in vivo test to assess the skin repair and anti-wrinkle effects.The 3D skin model(FulKutis®)was utilized to verify the effects of the PDRN essence on anti-inflammation,barrier repair,and anti-aging.In human test,a randomized,half-face comparison test with PDRN essence and the placebo was conducted with 33 Chinese subjects who met the test criteria.During the test,the skin conditions in terms of the a*value,facial red area ratio,and Transepidermal Water Loss(TEWL)were evaluated at 30 min,4 h,1 day,and 3 days;the skin conditions in terms of skin elasticity and firmness,skin wrinkles were evaluated at 42 days.Subjects’self-assessments were collected at 3 days and 42 days.The FulKutis■experiments demonstrated that the PDRN essence significantly decreased the levels of IL-1α,TNF-α,and PGE2,and improved the protein levels of Filaggrin,Loricrin,Collagen I,Collagen IV,and the thickness of the epidermal living cell layer.In the human study,the a*value,red area ratio,TEWL,and skin-related parameters(R2,R5,R7,F4,and skin wrinkles)on the test face side exhibited greater improvement compared to the control face side with statistically significant differences.Additionally,subject selfassessments revealed high satisfaction with the PDRN essence.The PDRN essence applied after non-ablative fractional laser treatment promotes barrier repair and soothing effects,shortens the recovery period,and enhances facial firmness and wrinkle improvement at the crow’s feet and undereye regions.No adverse events related to the tested cosmetics were observed.
文摘By compounding the cranberry extract,lavender flower extract,Kakadu plum extract,edelweiss flower/leaf extract and aloe vera extract into a composite plant extract(BIRC-Rosée WT100),the whitening effects of this composite plant extract in antioxidation,anti-inflammation,skin barrier maintenance,and inhibition of melanin production and transport were evaluated.By comparing the inhibitory effects on tyrosinase activity between the composite plant extract andα-arbutin,and then analyzing the decline of apparent chromaticity,apparent brightness and melanin content through 3D melanin skin model platform,the comprehensive whitening effect was evaluated and compared with that of kojic acid.The results showed that,the composite plant extract(BIRC-Rosée WT100)had a tyrosinase inhibition rate of 98.99%at the original concentration,which was much higher than that ofα-arbutin.In 3D melanin skin model,the apparent chromaticity and brightness could be effectively improved,and moreover,the total amount of melanin in the skin could be reduced,showing remarkable whitening effect.
文摘One being developed automatic sweep robot, need to estimate if anyone is on a certain range of road ahead then automatically adjust running speed, in order to ensure work efficiency and operation safety. This paper proposed a method using face detection to predict the data of image sensor. The experimental results show that, the proposed algorithm is practical and reliable, and good outcome have been achieved in the application of instruction robot.
文摘Objectives: Exposing skin to moderate ionic osmotic stress (MIOS) triggers several biochemical responses. The objective of this work is to reveal the mechanism triggered by MIOS on the skin surface. Furthermore, this work aims to study the involvement of the Nrf2 (nuclear factor erythroid-2-related factor 2) pathway, activated by MIOS, and its beneficial effect in protecting skin against stress via the stimulation of phase II enzymes. Methods: HaCaT cells and human skin organ culture were exposed to Dead Sea Water (DSW) as MIOS inducers and the induction of internal ROS elevation, Nrf2 translocation, mRNA gene expressions of the phase II enzymes, heme-oxygenase 1 (HO1), and Catalase (CAT) were determined. Results: Skin exposure to MIOS increases Nrf2 translocation to the nucleus, leading to increased levels of ROS, HO1, and CAT. Furthermore, exposing skin to MIOS promotes protection against UVB-related risks. This is demonstrated by attenuation of the expression of biomarkers, related to UVB-induced damage, Caspase-3, IL-8, and IL-1β. Conclusions: Skin exposure to MIOS leads to the activation of Nrf2 skin defense pathway and, therefore, could present beneficial advantages to human skin health, as demonstrated on human skin models. The beneficial effects of MIOS, induced by DSW are significantly superior to eq. NaCl brine, suggests that MIOS protection of skin against stress is partially related to specific mineral combinations.
基金supported by the National Key Research and Development Program of China(2020YFA0908200)the National Natural Science Foundation of China(T2225003)+3 种基金the Nanjing Medical Science and Technique Development Foundation(ZKX21019)the Clinical Trials from Nanjing Drum Tower Hospital(2022-LCYJ-ZD-01)the Key Research&Developement Program of Jiangsu Province(No.BE2022853)the Joint Fund of Henan Province Science and Technology R&D Program(Project NO.225200810021).
文摘The development of bionic skin is crucial for progressing our understanding of skin physiology and pathologies,while the precise replication of human skin features in skin models remains a challenge.In this study,we present a biomimetic,gender-specific skin model utilizing a gonads/epidermis-on-a-chip platform.This innovative approach seamlessly integrates ex vivo cultured human epidermal tissue and gonad cell aggregates into a microfluidic system,facilitating the exploration of the regulatory effects of sex hormones on the epidermis.Based on this platform,we have found that estradiol can enhance proliferation and inhibit apoptosis of keratinocytes.Conversely,testosterone notably induces keratinocyte differentiation,leading to epidermal hyperkeratosis.These observations align with the well-documented phenomenon that males possess thicker and more durable skin,while females’skin exhibits superior repair capabilities.Thus,we believe that our biomimetic gender-specific skin model can offer a promising avenue for obtaining higher-certainty evidence of human skin-related conditions and find important applications in the areas of cosmetics and biomedicine.
基金supported by Key R&D Program of Zhejiang Province(No.2023C01039).
文摘Realistic human skin rendering has been a long-standing challenge in computer graphics.Recently,biophysical-based skin rendering has received increasing attention,as it provides a more realistic skin-rendering and a more intuitive way to adjust the skin style.In this work,we present a novel heterogeneous biophysical-based volume rendering method for human skin that improves the realism of skin appearance while easily simulating various types of skin effects,including skin diseases,by modifying biological coefficient textures.Specifically,we introduce a two-layer skin representation by mesh deformation that explicitly models the epidermis and dermis with heterogeneous volumetric medium layers containing the corresponding spatially varying melanin and hemoglobin,respectively.Furthermore,to better facilitate skin acquisition,we introduced a learning-based framework that automatically estimates spatially varying biological coefficients from an albedo texture,enabling biophysical-based and intuitive editing,such as tanning,pathological vitiligo,and freckles.We illustrated the effects of multiple skinediting applications and demonstrated superior quality to the commonly used random walk skin-rendering method,with more convincing skin details regarding subsurface scattering.
文摘This paper presents a new face detection approach to real-time applications, which is based on the skin color model and the morphological filtering. First the non-skin color pixels of the input image are removed based on the skin color model in the YC rC b chrominance space, from which we extract candidate human face regions. Then a mathematical morphological filter is used to remove noisy regions and fill the holes in the candidate skin color regions. We adopt the similarity between the human face features and the candidate face regions to locate the face regions in the original image. We have implemented the algorithm in our smart media system. The experiment results show that this system is effective in real-time applications.
文摘Although multi-view monitoring techniques have been widely applied in skinned model reconstruction and movement analysis,traditional systems using high-performance Personal Computers(PCs),or industrial cameras are often prohibitive due to high costs and limited scalability.Here,we introduce an affordable,scalable multi-view image acquisition system for skinned model reconstruction in animal studies,utilizing consumer Android devices and a wireless network for synchronized monitoring named Rodent Arena Multi-View Monitor(RAMM).It uses smartphones as camera nodes with local data storage,enabling cost-effective scalability.Its custom synchronization solution and portability make it ideal for research and education in rodent behavior analysis,offering a practical alternative for institutions with limited budgets.Furthermore,the portability and flexibility of this system make it an ideal tool for rodent skinned model research based on multi-view image acquisition.To evaluate the performance,we perform an oscilloscope analysis to ensure effectiveness of synchronization.A 45-camera node setup is built to highlight RAMM’s cost efficiency and ease in constructing large-scale systems.Additionally,the data quality is validated using the Instant Neural Graphics Primitives(Instant-NGP)method.Remarkable results were achieved with a 30.49 dB PSNR by utilizing only 25 images with intrinsic and extrinsic parameters,fulfilling the requirements for well-synchronized data used in 3D representation algorithms.
基金partially supported by the National Natural Science Foundation of China(31930022,31771476,12026608,12042104,and 11871456)the Strategic Priority Project of CAS(XDB38040400)+1 种基金the National Key R&D Program of China(2017YFA0505500)JST Moonshot R&D program(JP MJMS2021 to L.C.).
文摘Skin,as the outmost layer of human body,is frequently exposed to environmental stressors including pollutants and ultraviolet(UV),which could lead to skin disorders.Generally,skin response process to ultraviolet B(UVB)irradiation is a nonlinear dynamic process,with unknown underlying molecular mechanism of critical transition.Here,the landscape dynamic network biomarker(lDNB)analysis of time series transcriptome data on 3D skin model was conducted to reveal the complicated process of skin response to UV irradiation at both molecular and network levels.The advanced l-DNB analysis approach showed that:(i)there was a tipping point before critical transition state during pigmentation process,validated by 3D skin model;(ii)13 core DNB genes were identified to detect the tipping point as a network biomarker,supported by computational assessment;(iii)core DNB genes such as COL7A1 and CTNNB1 can effectively predict skin lightening,validated by independent human skin data.Overall,this study provides new insights for skin response to repetitive UVB irradiation,including dynamic pathway pattern,biphasic response,and DNBs for skin lightening change,and enables us to further understand the skin resilience process after external stress.
基金financially supported by the National Natural Science Foundation of China (Grant No.51336006 and 51727811)
文摘Laser lipolysis can effectively treat obesity and its associated diseases, such as hypertension, fatty liver, and hyperlipidemia. However, currently available invasive laser lipolysis, which transmits laser to a fiber-optic catheter inserted into the subcutaneous tissue for irradiation through an incision, may cause hematomas, infections, and empyrosis. The current study presents a novel, noninvasive approach for laser lipolysis, which directly irradiates the intact skin surface without an incision and preferentially targets adipose tissue at the near-infrared band. High laser energy is necessary to damage adipocytes; however, this may carbonate and burn the dermis. Therefore, the introduction of skin cooling is essential to avoid unwanted hyperthermal injury and improve the threshold of radiant exposure. In the current study, we investigated a novel noninvasive approach assisted with skin cooling by establishing a homogeneous multi-layer skin model. In this method, light propagation in the skin was simulated by using the Monte Carlo method. Skin cooling was employed before laser irradiation to protect the epidermis from thermal damage, which was treated as a boundary condition based on Newton's law. The numerical results showed that the photons were deposited in the adipose layer more than in the other layers. Laser can effectively destroy adipose tissue at an energy density of >200 J/cm^2 at 1210 nm wavelength, whereas at least 300 J/cm^2 is required at 1064 nm to achieve the same effect. In this experiment, at >5 s pulse width, the selectivity of adipose was not obvious. Moreover, the results indicated that 60 ms R134a or R404a spray can effectively reduce the temperature of the epidermis. R404a exhibited a stronger cooling effect than R134a. Cold air cooling at -10 °C for 10 s could effectively decrease the skin temperature, and deeper cooling could be achieved by cold air cooling compared with cryogen spray cooling.
