Early mammalian embryogenesis represents a central question in life sciences,yet its molecular regulation has long been inferred primarily from transcriptomic and translatomic analyses.Recently,a study by Zhu et al.(2...Early mammalian embryogenesis represents a central question in life sciences,yet its molecular regulation has long been inferred primarily from transcriptomic and translatomic analyses.Recently,a study by Zhu et al.(2025)based on low-input proteomic approaches systematically charted protein dynamics from oocytes to blastocysts in both mice and humans,further interrogating molecular signatures of developmentally compromised human embryos at the single-embryo level.This work not only substantially expands proteome coverage during early development,but also reveals a pervasive uncoupling between transcriptional activation,translational initiation,and protein accumulation.These findings provide new perspectives on the relationship between zygotic genome activation(ZGA)and lineage specification.This paper discussed the central role of low-input proteomics in this study,highlighted its implications for reshaping current paradigms of early embryonic development,and considered its potential applications across broader areas of biomedical research.展开更多
文摘Early mammalian embryogenesis represents a central question in life sciences,yet its molecular regulation has long been inferred primarily from transcriptomic and translatomic analyses.Recently,a study by Zhu et al.(2025)based on low-input proteomic approaches systematically charted protein dynamics from oocytes to blastocysts in both mice and humans,further interrogating molecular signatures of developmentally compromised human embryos at the single-embryo level.This work not only substantially expands proteome coverage during early development,but also reveals a pervasive uncoupling between transcriptional activation,translational initiation,and protein accumulation.These findings provide new perspectives on the relationship between zygotic genome activation(ZGA)and lineage specification.This paper discussed the central role of low-input proteomics in this study,highlighted its implications for reshaping current paradigms of early embryonic development,and considered its potential applications across broader areas of biomedical research.
