This case study demonstrates a single-position supine approach for robotic retroperitoneal lymph node dissection(R-RPLDN)for the treatment of nonseminomatous germ cell tumors and residual masses after chemotherapy.We ...This case study demonstrates a single-position supine approach for robotic retroperitoneal lymph node dissection(R-RPLDN)for the treatment of nonseminomatous germ cell tumors and residual masses after chemotherapy.We performed a bilateral R-RPLDN in a 33-year-old man with nonseminomatous germ cell tumors and residual postchemotherapy masses.For this approach,the patient was placed in a steep Trendelenburg position,and a 5-port transperitoneal technique was used,with the robot docked so that the arms were oriented cephalad.This approach allowed simultaneous access to both sides of the retroperitoneum,thereby eliminating the need for bilateral lymphadenectomy and patient repositioning.Bilateral R-RPLDN was performed using a single-position supine approach.This versatile approach offers a less invasive,more efficient,and safer solution for removing residual postchemotherapy testicular cancer masses.展开更多
Liver X receptors(LXRs)are known as key transcription factors in lipid metabolism and have been reported to play an important role in T-cell proliferation.However,whether LXRs play a role in thymocyte development rema...Liver X receptors(LXRs)are known as key transcription factors in lipid metabolism and have been reported to play an important role in T-cell proliferation.However,whether LXRs play a role in thymocyte development remains largely unknown.Here,we demonstrated that LXRβdeficiency caused a reduction in single-positive(SP)thymocytes,whereas the transitions from the double-negative to SP stage were normal.Meanwhile,LXRβ-null SP thymocytes exhibited increased apoptosis and impairment of the IL-7Rα-Bcl2 axis.In addition,the LXR agonist T0901317 promoted the survival of SP thymocytes with enhanced IL-7Rαexpression in wild-type mice but not in LXRβ-deficient mice.Mechanistically,LXRβpositively regulated the expression of IL-7Rαvia direct binding to the Il7r allele in SP thymocytes,and forced expression of IL-7Rαor Bcl2 restored the survival of LXRβ-defective SP thymocytes.Thus,our results indicate that LXRβfunctions as an important transcription factor upstream of IL-7Rαto promote the survival of SP thymocytes.展开更多
基金supported by the Natural Science Foundation of Hunan Province(2022JJ30908)Innovation Guidance Project of Clinical Medical Technology of Hunan Province(2021SK53710).
文摘This case study demonstrates a single-position supine approach for robotic retroperitoneal lymph node dissection(R-RPLDN)for the treatment of nonseminomatous germ cell tumors and residual masses after chemotherapy.We performed a bilateral R-RPLDN in a 33-year-old man with nonseminomatous germ cell tumors and residual postchemotherapy masses.For this approach,the patient was placed in a steep Trendelenburg position,and a 5-port transperitoneal technique was used,with the robot docked so that the arms were oriented cephalad.This approach allowed simultaneous access to both sides of the retroperitoneum,thereby eliminating the need for bilateral lymphadenectomy and patient repositioning.Bilateral R-RPLDN was performed using a single-position supine approach.This versatile approach offers a less invasive,more efficient,and safer solution for removing residual postchemotherapy testicular cancer masses.
基金This work was supported by grants from the National Key Research and Development Program of China(No.2016YFA0502203 to X.Z.and No.2016YFA0502204 to Y.W.)the National Natural Science Foundation of China(No.81571537 to T.Z.,No.31770949 to X.Z.,No.31770972 to Z.X.,and No.81571604 to J.Z.)the Chongqing Basic and Frontier Research Project(No.cstc2015jcyjBX0086 to H.He.).
文摘Liver X receptors(LXRs)are known as key transcription factors in lipid metabolism and have been reported to play an important role in T-cell proliferation.However,whether LXRs play a role in thymocyte development remains largely unknown.Here,we demonstrated that LXRβdeficiency caused a reduction in single-positive(SP)thymocytes,whereas the transitions from the double-negative to SP stage were normal.Meanwhile,LXRβ-null SP thymocytes exhibited increased apoptosis and impairment of the IL-7Rα-Bcl2 axis.In addition,the LXR agonist T0901317 promoted the survival of SP thymocytes with enhanced IL-7Rαexpression in wild-type mice but not in LXRβ-deficient mice.Mechanistically,LXRβpositively regulated the expression of IL-7Rαvia direct binding to the Il7r allele in SP thymocytes,and forced expression of IL-7Rαor Bcl2 restored the survival of LXRβ-defective SP thymocytes.Thus,our results indicate that LXRβfunctions as an important transcription factor upstream of IL-7Rαto promote the survival of SP thymocytes.
