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Deep Learning-Based Toolkit Inspection:Object Detection and Segmentation in Assembly Lines
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作者 Arvind Mukundan Riya Karmakar +1 位作者 Devansh Gupta Hsiang-Chen Wang 《Computers, Materials & Continua》 2026年第1期1255-1277,共23页
Modern manufacturing processes have become more reliant on automation because of the accelerated transition from Industry 3.0 to Industry 4.0.Manual inspection of products on assembly lines remains inefficient,prone t... Modern manufacturing processes have become more reliant on automation because of the accelerated transition from Industry 3.0 to Industry 4.0.Manual inspection of products on assembly lines remains inefficient,prone to errors and lacks consistency,emphasizing the need for a reliable and automated inspection system.Leveraging both object detection and image segmentation approaches,this research proposes a vision-based solution for the detection of various kinds of tools in the toolkit using deep learning(DL)models.Two Intel RealSense D455f depth cameras were arranged in a top down configuration to capture both RGB and depth images of the toolkits.After applying multiple constraints and enhancing them through preprocessing and augmentation,a dataset consisting of 3300 annotated RGB-D photos was generated.Several DL models were selected through a comprehensive assessment of mean Average Precision(mAP),precision-recall equilibrium,inference latency(target≥30 FPS),and computational burden,resulting in a preference for YOLO and Region-based Convolutional Neural Networks(R-CNN)variants over ViT-based models due to the latter’s increased latency and resource requirements.YOLOV5,YOLOV8,YOLOV11,Faster R-CNN,and Mask R-CNN were trained on the annotated dataset and evaluated using key performance metrics(Recall,Accuracy,F1-score,and Precision).YOLOV11 demonstrated balanced excellence with 93.0%precision,89.9%recall,and a 90.6%F1-score in object detection,as well as 96.9%precision,95.3%recall,and a 96.5%F1-score in instance segmentation with an average inference time of 25 ms per frame(≈40 FPS),demonstrating real-time performance.Leveraging these results,a YOLOV11-based windows application was successfully deployed in a real-time assembly line environment,where it accurately processed live video streams to detect and segment tools within toolkits,demonstrating its practical effectiveness in industrial automation.The application is capable of precisely measuring socket dimensions by utilising edge detection techniques on YOLOv11 segmentation masks,in addition to detection and segmentation.This makes it possible to do specification-level quality control right on the assembly line,which improves the ability to examine things in real time.The implementation is a big step forward for intelligent manufacturing in the Industry 4.0 paradigm.It provides a scalable,efficient,and accurate way to do automated inspection and dimensional verification activities. 展开更多
关键词 Tool detection image segmentation object detection assembly line automation Industry 4.0 Intel RealSense deep learning toolkit verification RGB-D imaging quality assurance
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Numerical study on the failure characteristics and reinforcement effects of shield segmental linings subjected to localized overload
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作者 Long ZHOU Zhiguo YAN Mengqi ZHU 《Journal of Zhejiang University-Science A(Applied Physics & Engineering)》 2025年第10期950-966,共17页
When only a portion of the shield lining structures in a full-line tunnel are overloaded,their bearing and failure characteristics are significantly different from those in the full-line overloaded case.In existing st... When only a portion of the shield lining structures in a full-line tunnel are overloaded,their bearing and failure characteristics are significantly different from those in the full-line overloaded case.In existing studies,one or several segmental lining rings have been studied,with overload applied to selected lining rings to analyze the performance evolution of the lining structures;however,this approach fails to reveal the bearing and failure characteristics of shield lining rings under localized overload.To address this research gap,we employ 3D finite element modeling to investigate the mechanical performance and failure mechanisms of shield segmental linings under localized overload conditions,and compare the results with full-line overload scenarios.Additionally,the impact of reinforcing shield segmental linings with steel rings is studied to address issues arising from localized overloads.The results indicate that localized overloads lead to significant ring joint dislocation and higher stress on longitudinal bolts,potentially causing longitudinal bolt failure.Furthermore,the overall deformation of lining rings,segmental joint opening,and stress in circumferential bolts and steel bars is lower compared to full-line overloads.For the same overload level,the convergence deformation of the lining under full-line overload is 1.5 to 2.0 times higher than that under localized overload.For localized overload situations,a reinforcement scheme with steel rings spanning across two adjacent lining rings is more effective than installing steel rings within individual lining rings.This spanning ring reinforcement strategy not only enhances the structural rigidity of each ring,but also limits joint dislocation and reduces stress on longitudinal bolts,with the reduction in maximum ring joint dislocation ranging from 70%to 82%and the reduction in maximum longitudinal bolt stress ranging from 19%to 57%compared to reinforcement within rings. 展开更多
关键词 Shield segmental lining Localized overload Failure characteristics Steel ring reinforcement
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Analyzing the strengthening effect of steel-ultra high performance concrete composite on segmental linings
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作者 Renpeng CHEN Meng FAN +4 位作者 Hongzhan CHENG Huaina WU Yang ZHANG Bingyong GAO Shiqiang RUAN 《Journal of Zhejiang University-Science A(Applied Physics & Engineering)》 2025年第6期558-572,共15页
This study aims to assess the comprehensive strengthening effect of a steel-ultra high performance concrete(UHPC)composite strengthening method.The axial force-moment interaction curve(N-M curve)was calculated in a no... This study aims to assess the comprehensive strengthening effect of a steel-ultra high performance concrete(UHPC)composite strengthening method.The axial force-moment interaction curve(N-M curve)was calculated in a novel way,using cross-sectional strains at ultimate states as well as real-time stress measurements for each material.The enclosed area of the N-M curve was defined as a comprehensive performance index for the system.We validate our approach with comparisons to numerical modeling and full-scale four-point bending experiments.Additionally,strengthening effects were compared for different sagging and hogging moments based on material stress responses,and the impact of various strengthening parameters was analyzed.We find that the N-M curve of the strengthened cross-section envelops that of the un-strengthened cross-section.Notably,improvements in flexural capacity are greater under sagging moments during the large eccentric failure stage,and greater under hogging moments during the small eccentric failure stage.This discrepancy is attributed to the strength utilization of strengthening materials.These findings provide a reference for understanding the strengthening effects and parameters of steel-UHPC composite. 展开更多
关键词 Strengthening effects Steel-ultra high performance concrete(UHPC)composite Axial force-moment interaction curve Shield segmental linings
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Studies on the key parameters in segmental lining design 被引量:2
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作者 Zhenchang Guan Tao Deng +1 位作者 Gang Wang Yujing Jiang 《Journal of Rock Mechanics and Geotechnical Engineering》 SCIE CSCD 2015年第6期674-683,共10页
The uniform ring model and the shell-spring model for segmental lining design are reviewed in thisarticle. The former is the most promising means to reflect the real behavior of segmental lining, while thelatter is th... The uniform ring model and the shell-spring model for segmental lining design are reviewed in thisarticle. The former is the most promising means to reflect the real behavior of segmental lining, while thelatter is the most popular means in practice due to its simplicity. To understand the relationship and thedifference between these two models, both of them are applied to the engineering practice of FuzhouMetro Line I, where the key parameters used in both models are described and compared. The effectiveratio of bending rigidity h reflecting the relative stiffness between segmental lining and surroundingground and the transfer ratio of bending moment x reflecting the relative stiffness between segment andjoint, which are two key parameters used in the uniform ring model, are especially emphasized. Thereasonable values for these two key parameters are calibrated by comparing the bending momentscalculated from both two models. Through case studies, it is concluded that the effective ratio of bendingrigidity h increases significantly with good soil properties, increases slightly with increasing overburden,and decreases slightly with increasing water head. Meanwhile, the transfer ratio of bending moment xseems to only relate to the properties of segmental lining itself and has a minor relation with the groundconditions. These results could facilitate the design practice for Fuzhou Metro Line I, and could alsoprovide some references to other projects with respect to similar scenarios. 展开更多
关键词 segmental lining Uniform ring model Shell-spring model Effective ratio of bending rigidity Transfer ratio of bending moment
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Prototype test study on mechanical characteristics of segmental lining structure of underwater railway shield tunnel 被引量:6
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作者 He Chuan Feng Kun Yan Qixiang 《Engineering Sciences》 EI 2014年第2期65-74,共10页
Based on the first unde rwater railway shield tunnel, the Shiziyang shield tunnel of Guangzhou Zhu- jiang River, the prototype test was carried out against its segmental lining structure by using "multi-function shie... Based on the first unde rwater railway shield tunnel, the Shiziyang shield tunnel of Guangzhou Zhu- jiang River, the prototype test was carried out against its segmental lining structure by using "multi-function shield tunnel structure test system". And the mechanical characteristics of segmental lining structure using straight assembling and staggered assembling were studied deeply. The results showed that, the mechanical characteristics of segmental lining structure varied with the water pressures; especially after cracking, the high water pressure played a significant role in slowing down the growing inner force and deformation. It also testi- fied that the failure characteristics varied with straight assembling structure and staggered assembling structure. Shear thilurc often occurred near longitudinal seam when using straight assembling. 展开更多
关键词 underwater railway shield tunnel segmental lining structure prototype test tenon joint failure characteristics
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DCNN Based Finger Knuckle Print Recognition Using C-ROI Morphological Segmentation and Derivative Line Extraction
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作者 Sathiya L Palanisamy V 《China Communications》 2025年第11期144-160,共17页
One of the evolving hand biometric features considered so far is finger knuckle printing,because of its ability towards unique identification of individuals.Despite many attempts have been made in this area of researc... One of the evolving hand biometric features considered so far is finger knuckle printing,because of its ability towards unique identification of individuals.Despite many attempts have been made in this area of research,the accuracy of the recognition model remains a major issue.To overcome this problem,a novel biometric-based method,named fingerknuckle-print(FKP),has been developed for individual verification.The proposed system carries key steps such as preprocessing,segmentation,feature extraction and classification.Initially input FKP image is fed into the preprocessing stage where colour images are converted to gray scale image for augmenting the system performance.Afterwards,segmentation process is carried out with the help of CROI(Circular Region of Interest)and Morphological operation.Then,feature extraction stage is carried out using Gabor-Derivative line approach for extracting intrinsic features.Finally,DCNN(Deep Convolutional Neural Network)is trained for the processed knuckle images to recognize imposter and genuine individuals.Extensive experiments on standard FKP database demonstrates that the proposed method attains considerable improvement compared with state-of-the-art methods.The overall accuracy attained for the proposed methodology is 95.6%which is achieved better than the existing techniques. 展开更多
关键词 ACCURACY deep convolutional neural network derivative line method gabor filter morphological segmentation sensitivity
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Optimal design scheme based on the segmental transmission line methodology for high-frequency interconnection in 3D micro/nano integration
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作者 Chenye Li Zhensong Li Min Miao 《Nanotechnology and Precision Engineering》 EI CAS CSCD 2020年第3期174-186,共13页
With the continuous growth of signal frequency and package density, discontinuity of the high-frequency interconnection in 3 D micro/nano integration becomes unavoidable, which results in serious signal integrity prob... With the continuous growth of signal frequency and package density, discontinuity of the high-frequency interconnection in 3 D micro/nano integration becomes unavoidable, which results in serious signal integrity problems. Traditional interconnection design schemes, such as termination and shielding/isolation, cannot meet the requirements under the unified constraints of specific cost, space occupancy, and performance. In this study, a transmission line design optimization scheme based on the segmental transmission line(STL) methodology is proposed. The genetic algorithm is used to select the optimal segment structure parameters of the transmission line to construct an STL with satisfying transmission performance or meet the specific signal amplitude adjustment requirements. This scheme can be adapted to various signal transmission scenarios to significantly improve the signal loss caused by reflection or other negative electromagnetic factors and meet the requirements for the modeling of discontinuous transmission lines. The simulation results show that this scheme is effective in the design scenario of performance improvement or equivalent modeling of discontinuous transmission lines and has significant advantages in circuit area reduction. 展开更多
关键词 Discontinuous transmission line segmental transmission line Genetic algorithm Equivalent model
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Prototype Loading Tests on Full-Ring Segmental Lining of Rectangular Shield Tunnel
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作者 ZHU Yeting ZHANG Zixin +1 位作者 HUANG Xin ZHANG Guanjun 《Journal of Shanghai Jiaotong university(Science)》 EI 2018年第6期746-757,共12页
A series of full-scale loading tests are performed for a prospective subway tunnel with a rectangular shape including two reliability tests: one stagger-jointed three-ring reliability test, and one ultimate failure te... A series of full-scale loading tests are performed for a prospective subway tunnel with a rectangular shape including two reliability tests: one stagger-jointed three-ring reliability test, and one ultimate failure test on a single ring. Comprehensive measuring programs are designed to record the deformation of both lining structure and joints and the stresses of concrete, bolts and reinforcements. Experimental results show that in both the single-ring and three-ring loading cases, the long sides of tunnel cross section bend inwards while the short sides of tunnel cross section bend outwards. The inner part of joints opens while the outer part of joints closes at places experiencing positive moment and vice versa. Joint's rotational stiffness varies at different locations. Concrete cracking and crushing are the chief damage modes, and they are closely related to the distribution of bending moment. Stagger-jointed fabrication significantly increases the overall rigidity of lining system, which thereby greatly reduces the deformation of both concrete lining and joints in comparison with the single-ring case. It is shown that the routinely-used uniform rigidity model is conservative and the preliminary design can be optimized by applying an effective rigidity ratio(ERR) of 0.5. 展开更多
关键词 segmental lining prototype loading test rectangular shield tunnel effective rigidity ratio(ERR) ultimate failure
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Mapping of QTLs Related to Grain Weight Using Chromosome Single Segment Substitution Lines in Rice 被引量:1
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作者 王军 周勇 +5 位作者 杨杰 朱金燕 范方军 李文奇 梁国华 仲维功 《Agricultural Science & Technology》 CAS 2014年第8期1288-1294,共7页
Grain weight, one of the major factors determining rice yield, is a typical quantitative trait control ed by multiple genes. With Guangluai 4 as recipient and Nipponbare as donor, a population of 119 chromosome single... Grain weight, one of the major factors determining rice yield, is a typical quantitative trait control ed by multiple genes. With Guangluai 4 as recipient and Nipponbare as donor, a population of 119 chromosome single segment substitution lines had been developed. Correlation analysis between grain weight and grain shape by SPSS revealed that 1 000-grain weight shared extremely significant posi-tive correlation with grain length and length-width ratio, but no significant correlation with grain width and thickness. The QTL analysis of grain weight was carried out using one-way analysis of variance and Dunnett's test. Nineteen stable QTLs re-sponsible for grain weight were identified over two years. Al 19 QTLs were identi-fied on al chromosomes except for chromosome 10 and 12 at a significance level of P≤0.001. Among them, 10 QTLs had a positive effect and were derived from the Nipponbare al ele, the additive effect of these QTLs ranged from 0.49 to 2.74 g, and the contributions of the additive effects ranged from 2.00% to 11.05%. Another 9 QTLs had a negative effect and were al derived from Guangluai 4 al ele, the ad-ditive effect of these QTLs ranged from 0.60 to 2.35 g, and the contributions of the additive effects ranged from 2.40% to 9.84%. The results provide a basis for the fine mapping and gene cloning of novel locus associated with rice grain weight. 展开更多
关键词 RICE Chromosome single segment substitution lines Quantitative trait loci 1 000-grain weight Substitution mapping
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QTLs Mapping for Salinity Tolerance at Seedling Stage or Rice(Oryza sativa L.) Using Chromosome Segment Substitution Lines
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作者 林静 张所兵 +2 位作者 张云辉 汪迎节 方先文 《Agricultural Science & Technology》 CAS 2017年第12期2209-2211,共3页
In this study, a population of chromosome segment substitution lines (CSSLs) derived from the cross between 9311 (indica) and Nipponbare (japonica) was employed to map the quantitative trait loci (QTLs) for sa... In this study, a population of chromosome segment substitution lines (CSSLs) derived from the cross between 9311 (indica) and Nipponbare (japonica) was employed to map the quantitative trait loci (QTLs) for salt tolerance under the salt stress simulated with 0.5% NaCI, using survival rate as the index. The data were analyzed by QTL IciMapping v3.1, and the results showed that one QTL (QSsr3) related to salt tolerance was located in the vicinity of the marker RM1350 on chromosome 3, into a genetic interval of 113.2-132.8 cM, with a contribution rate of 17.75%. The additive effect was 10.9, indicating that the QTL derived from the parent Nipponbare improved the salt tolerance of rice at seedling stage. This study will provide a theoretical basis for the selection of salt tolerant rice germplasm. 展开更多
关键词 RICE Salt tolerance Chromosome segment substitution lines (CSSLs) Quantitative trait loci (QTLs) mapping
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Mapping QTLs Associated with Sheath Blight Resistance Using Chromosome Segment Substitution Lines of Rice(Oryza sativa L.)
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作者 林静 张所兵 +2 位作者 张云辉 汪迎节 方先文 《Agricultural Science & Technology》 CAS 2014年第5期756-759,共4页
In this study, a population of 119 chromosome segment substitution lines (CSSLs) derived from backcross between indica 9311 and japonica Nipponbare was employed to map quantitative trait loci (QTL) associated with... In this study, a population of 119 chromosome segment substitution lines (CSSLs) derived from backcross between indica 9311 and japonica Nipponbare was employed to map quantitative trait loci (QTL) associated with sheath blight resis-tance in rice with toothpick inoculation method. A total of three sheath blight resis-tance-associated QTLs (qsb8-1, qsb8-2 and qsb8-3) were identified, which were lo-cated on adjacent molecular markers RM3262, RM5485 and RM3496 of chromo-some 8; the genetic interval was 81.7cM-91.7cM, 91.7cM-108.1cM and 108.1cM-119.6cM, respectively. The additive effect of qsb8-2 was negative, indicating that sheath blight resistance of susceptible parent harboring qsb8-2 fragment was en-hanced; additive effects of qsb8-1 and qsb8-3 were positive, indicating that sheath blight resistance of susceptible parent harboring qsb8-1 and qsb8-3 fragments was reduced. 展开更多
关键词 RICE Sheath blight resistance Chromosome segment substitution lines(CSSLs) Quantitative trait locus (QTL) mapping
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Development and high-throughput genotyping of substitution lines carting the chromosome segments of indica 9311 in the background of japonica Nipponbare 被引量:13
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作者 Hua Zhang Qiang Zhao +7 位作者 Zhi-Zhong Sun Chang-Quan Zhang Qi Feng Shu-Zhu Tang Guo-Hua Liang Ming-Hong Gu Bin Han Qiao-Quan Liu 《Journal of Genetics and Genomics》 SCIE CAS CSCD 2011年第12期603-611,共9页
Chromosome segment substitution lines(CSSLs) are useful for the precise mapping of quantitative trait loci(QTLs) and dissection of the genetic basis of complex traits.In this study,two whole-genome sequenced rice ... Chromosome segment substitution lines(CSSLs) are useful for the precise mapping of quantitative trait loci(QTLs) and dissection of the genetic basis of complex traits.In this study,two whole-genome sequenced rice cultivars,the japonica Nipponbare and indica 9311 were used as recipient and donor,respectively.A population with 57 CSSLs was developed after crossing and back-crossing assisted by molecular markers, and genotypes were identified using a high-throughput resequencing strategy.Detailed graphical genotypes of 38 lines were constructed based on resequencing data.These CSSLs had a total of 95 substituted segments derived from indica 9311,with an average of about 2.5 segments per CSSL and eight segments per chromosome,and covered about 87.4%of the rice whole genome.A multiple linear regression QTL analysis mapped four QTLs for 1000-grain weight.The largest-effect QTL was located in a region on chromosome 5 that contained a cloned major QTL GW5/qSW5 for grain size in rice.These CSSLs with a background of Nipponbare may provide powerful tools for future whole-genome 展开更多
关键词 Oryza sativa L. Chromosome segment substitution lines(CSSLs) Molecular marker-assisted selection High-throughput resequencing
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Identification of QTL for kernel number-related traits in a rice chromosome segment substitution line and fine mapping of qSP1 被引量:4
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作者 Fuying Ma Xiaoyan Zhu +8 位作者 Hui Wang Shiming Wang Guoqing Cui Ting Zhang Zhenglin Yang Guanghua He Yinghua Ling Nan Wang Fangming Zhao 《The Crop Journal》 SCIE CAS CSCD 2019年第4期494-503,共10页
A chromosome segment substitution line (CSSL) is a powerful tool for combining quantitative trait locus (QTL) mapping with the pyramiding of desirable alleles. The rice CSSL Z1364 with increased kernel number was iden... A chromosome segment substitution line (CSSL) is a powerful tool for combining quantitative trait locus (QTL) mapping with the pyramiding of desirable alleles. The rice CSSL Z1364 with increased kernel number was identified in a BC3F8 population derived from a cross of Nipponbare as the recipient with Xihui 18 as the donor parent. Z1364 carried three substitution segments distributed on chromosomes 1, 6, and 8. The mean substitution length was 1.19 Mb. Of 17 QTL identified on the substitution segments, qSP1 for spikelets per panicle, qSSD1 for seed-set density, and qNSB1 for number of secondary branches explained respectively 57.34%, 87.7%, and 49.44% of the corresponding phenotypic variance and were all linked to RM6777. Chi-square analysis showed that the increased kernel number in Z1364 was inherited recessively by a single gene. By fine mapping, qSP1 was delimited to a 50-kb region on the short arm of chromosome 1. Based on DNA sequence, a previously uncharacterized rice homolog of Arabidopsis thaliana AT4G32551 was identified as a candidate gene for qSP1 in which mutation increases the number of spikelets and kernels in Z1364. qSP1 was expressed in all tissues, but particularly in 1-cm panicles. The expression levels of OsMADS22, GN1A, and DST were upregulated and those of LAX2, GNP1, and GHD7 were downregulated in Nipponbare. These results provide a foundation for functional research on qSP1. 展开更多
关键词 RICE CHROMOSOME segment substitution line Increased number of KERNELS qSP1 QTL mapping for yield traits
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Identification of QTLs for Plant Height and Its Components by Using Single Segment Substitution Lines in Rice (Oryza sativa) 被引量:6
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作者 Akshay TALUKDAR 《Rice science》 SCIE 2005年第3期151-156,共6页
QTLs for plant height and its components on the substituted segments of fifty-two single segment substitution lines (SSSLs) in rice were identified through t-test (P〈0.001) for comparison between each SSSL and re... QTLs for plant height and its components on the substituted segments of fifty-two single segment substitution lines (SSSLs) in rice were identified through t-test (P〈0.001) for comparison between each SSSL and recipient parent Huajingxian 74. On the 14 substituted segments, 24 QTLs were detected, 10 for plant height, 2 for panicle length, 4 for length of the first internode from the top, 5 for length of the second internode from the top and 3 for length of the third internode from the top, respectively. All these QTLs were distributed on nine rice chromosomes except chromosomes 5, 9 and 11. The additive effect ranged from -4.08 to 3.98 cm, and the additive effect percentages varied from -19.35% to 10.43%. 展开更多
关键词 RICE single segment substitution line plant height quantitative trait locus
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Unconditional and Conditional QTL Mapping for Tiller Numbers at Various Stages with Single Segment Substitution Lines in Rice(Oryza sativa L.) 被引量:6
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作者 ZHAO Fang-ming LIU Gui-fu ZHU Hai-tao DING Xiao-hua ZENG Rui-zhen ZHANG Ze-min LI Wen-tao ZHANG Gui-quan 《Agricultural Sciences in China》 CAS CSCD 2008年第3期257-265,共9页
Tiller is one of the most important agronomic traits which influences quantity and quality of effective panicles and finally influences yield in rice. It is important to understand "static" and "dynamic" informati... Tiller is one of the most important agronomic traits which influences quantity and quality of effective panicles and finally influences yield in rice. It is important to understand "static" and "dynamic" information of the QTLs for tillers in rice. This work was the first time to simultaneously map unconditional and conditional QTLs for tiller numbers at various stages by using single segment substitution lines in rice. Fourteen QTLs for tiller number, distributing on the corresponding substitution segments of chromosomes 1, 2, 3, 4, 6, 7 and 8 were detected. Both the number and the effect of the QTLs for tiller number were various at different stages, from 6 to 9 in the number and from 1.49 to 3.49 in the effect, respectively. Tiller number QTLs expressed in a time order, mainly detected at three stages of 0-7 d, 14-21 d and 35-42 d after transplanting with 6 positive, 9 random and 6 negative expressing QTLs, respectively. Each of the QTLs expressed one time at least during the whole duration of rice. The tiller number at a specific stage was determined by sum of QTL effects estimated by the unconditional method, while the increasing or decreasing number in a given time interval was controlled by the total of QTL effects estimated by the conditional method. These results demonstrated that it is highly effective and accurate for mapping of the QTLs by using single segment substitution lines and the conditional analysis methodology. 展开更多
关键词 single segment substitution line unconditional QTL mapping conditional QTL mapping tiller number RICE
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Identification of long-grain chromosome segment substitution line Z744 and QTL analysis for agronomic traits in rice 被引量:7
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作者 MA Fu-ying DU Jie +7 位作者 WANG Da-chuan WANG Hui ZHAO Bing-bing HE Guang-hua YANG Zheng-lin ZHANG Ting WU Ren-hong ZHAO Fang-ming 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2020年第5期1163-1169,共7页
Length of grain affects the appearance, quality, and yield of rice. A rice long-grain chromosome segment substitution line Z744, with Nipponbare as the recipient parent and Xihui 18 as the donor parent, was identified... Length of grain affects the appearance, quality, and yield of rice. A rice long-grain chromosome segment substitution line Z744, with Nipponbare as the recipient parent and Xihui 18 as the donor parent, was identified. Z744 contains a total of six substitution segments distributed on chromosomes(Chrs.) 1, 2, 6, 7, and 12, with an average substitution length of 2.72 Mb. The grain length, ratio of length to width, and 1 000-grain weight of Z744 were significantly higher than those in Nipponbare. The plant height, panicle number, and seed-set ratio in Z744 were significantly lower than those in Nipponbare, but they were still 78.7 cm, 13.5 per plant, and 86.49%, respectively. Furthermore, eight QTLs of different traits were identified in the secondary F2 population, constructed by Nipponbare and Z744 hybridization. The grain weight of Z744 was controlled by two synergistic QTLs(qGWT1 and q GWT7) and two subtractive QTLs(qGWT2 and qGWT6), respectively. The increase in the grain weight of Z744 was caused mainly by the increase in grain length. Two QTLs were detected, qGL1 and qGL7-3, which accounted for 25.54 and 15.58% of phenotypic variation, respectively. A Chi-square test showed that the long-grain number and the short-grain number were in accordance with the 3:1 separation ratio, which indicates that the long grain is dominant over the short-grain and Z744 was controlled mainly by the principal effect qGL1. These results offered a good basis for further fine mapping of qGL1 and further dissection of other QTLs into single-segment substitution lines. 展开更多
关键词 RICE CHROMOSOME segment SUBSTITUTION line GRAIN length QTL
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Identification of Multiple Alleles at the Wx Locus and Development of Single Segment Substitution Lines for the Alleles in Rice 被引量:5
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作者 Akshay TALUKDAR 《Rice science》 SCIE 2006年第1期9-14,共6页
The microsatellite markers 484/485 and 484/W2R were used to identify the multiple alleles at the Wx locus in rice germplasm. Fifteen alleles were identified in 278 accessions by using microsatellite class and G-T poly... The microsatellite markers 484/485 and 484/W2R were used to identify the multiple alleles at the Wx locus in rice germplasm. Fifteen alleles were identified in 278 accessions by using microsatellite class and G-T polymorphism. Among these alleles, (CT)12-G, (CT)15-G, (CT)16-G, (CT)17-G, (CT)18-G and (CT)21-G have not been reported. Seventy-two single-segment substitution lines (SSSLs) carrying different alleles at the Wx locus were developed by using Huajingxian 74 with the (CT)11-G allele as a recipient and 20 accessions containing 12 different alleles at the Wx locus as donors. The estimated length of the substituted segments ranged from 2.2 to 77.3 cM with an average of 17.4 cM. 展开更多
关键词 RICE waxy gene single segment substitution line allelic variation molecular marker-assisted selection
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Detection of QTL for Cold Tolerance at Bud Bursting Stage Using Chromosome Segment Substitution Lines in Rice (Oryza sativa) 被引量:4
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作者 LIN Jing ZHU Wen-yin ZHANG Ya-dong ZHU Zhen ZHAO Ling CHEN Tao ZHAO Qing-yong ZHOU Li-hui FANG Xian-wen WANG yan-ping WANG Cai-lin 《Rice science》 SCIE 2011年第1期71-74,共4页
Ab The cold tolerance at the bud bursting stage (CTB) was evaluated at 5℃ by using a set of 95 chromosome segment substitution lines (CSSLs) derived from an indica rice 9311 and a japonica rice Nipponbare with a ... Ab The cold tolerance at the bud bursting stage (CTB) was evaluated at 5℃ by using a set of 95 chromosome segment substitution lines (CSSLs) derived from an indica rice 9311 and a japonica rice Nipponbare with a genetic background of 9311. The result showed that six CSSLs had slightly stronger effect on CTB than 9311. Total four quantitative trait loci (QTLs) for CTB were preliminary mapped on chromosomes 5 and 7 by substitution mapping, qCTB-5-1, qCTB-5-2 and qCTB-5-3 were mapped in the region of RM267-RM1237, RM2422-RM6054 and RM3321-RM1054, which were 21.3 cM, 27.4 cM and 12.7 cM in genetic distance on rice chromosome 5, respectively, qCTB-7 was mapped in a 6.8-cM region of RM11-RM2752 on rice chromosome 7. 展开更多
关键词 chromosome segment substitution lines cold tolerance bud bursting stage substitution mapping RICE
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Development of Chromosomal Segment Substitution Lines from a Backcross Recombinant Inbred Population of Interspecific Rice Cross 被引量:4
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作者 CHEN Jie Hafeez Ur Rahman BUGHIO +3 位作者 CHEN Da-zhou LIU Guang-jie ZHENG Kang-le ZHUANG Jie-yun 《Rice science》 SCIE 2006年第1期15-21,共7页
A backcross recombinant inbred line population consisting of 202 lines was developed from Xieqingzao B//Xieqingzao B / Dongxiang wild rice. The population was assayed with DNA markers and phenotyped on planthopper res... A backcross recombinant inbred line population consisting of 202 lines was developed from Xieqingzao B//Xieqingzao B / Dongxiang wild rice. The population was assayed with DNA markers and phenotyped on planthopper resistance and yield traits. A linkage map consisting of 119 DNA markers and spanned for 1188 cM over the 12 rice chromosomes was constructed. Thirty-two chromosomal segment substitution lines were selected based on the percentage of Xieqingzao B allele at marker loci. These lines are of great potential for gene mapping and alien gene introgression. 展开更多
关键词 alien introgression DNA marker Dongxiang wild rice chromosomal segment substitution line
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QTL Mapping for Rice RVA Properties Using High-Throughput Re-sequenced Chromosome Segment Substitution Lines 被引量:2
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作者 ZHANG Chang-quan HU Bing +5 位作者 ZHU Kong-zhi ZHANG Hua LENG Ya-lin TANG Shu-zhu GU Ming-hong LIU Qiao-quan 《Rice science》 SCIE 2013年第6期407-414,共8页
The rapid visco analyser (RVA) profile is an important factor for evaluation of the cooking and eating quality of rice. To improve rice quality, the identification of new quantitative trait loci (QTLs) for RVA pro... The rapid visco analyser (RVA) profile is an important factor for evaluation of the cooking and eating quality of rice. To improve rice quality, the identification of new quantitative trait loci (QTLs) for RVA profiling is of great significance. We used a japonica rice cultivar Nipponbare as the recipient and indica rice 9311 as the donor to develop a population containing 38 chromosome segment substitution lines (CSSLs) genotyped by a high-throughput re-sequencing strategy. In this study, the population and the parent lines, which contained similar apparent amylose contents, were used to map the QTLs of RVA properties including peak paste viscosity (PKV), hot paste viscosity (HPV), cool paste viscosity (CPV), breakdown viscosity (BKV), setback viscosity (SBV), consistency viscosity (CSV), peak time (PET) and pasting temperature (PAT). QTL analysis was carried out using one-way analysis of variance and Dunnett's test, and stable QTLs were identified over two years and under two environments. We identified 10 stable QTLs: qPKV2-1, qSBV2-1; qPKV5-1, qHPV5-1, qCPV5-1; qPKV7-1, qHPV7-1, qCPV7-1, qSBV7-1; and qPKV8-1 on chromosomes 2, 5, 7 and 8, respectively, with contributions ranging from -95.6% to 47.1%. Besides, there was pleiotropy in the QTLs on chromosomes 2, 5 and 7. 展开更多
关键词 RICE chromosome segment substitution line rapid visco analyzer profile quantitative traitlocus substitution mapping
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