Over the past three decades,fluorescence imaging has evolved from a descriptive tool into a precision instrument for dissecting biology at the molecular scale.1 This transformation has been propelled by parallel advan...Over the past three decades,fluorescence imaging has evolved from a descriptive tool into a precision instrument for dissecting biology at the molecular scale.1 This transformation has been propelled by parallel advances in optical instrumentation,such as super resolution micros-copy2−4 and single molecule tracking,5 and in the design of functional small molecule fluorophores.With the latest imaging technologies achieving near-molecular spatial reso-lution and submillisecond temporal precision,we are approaching the long-sought goal of interrogating biological systems at the molecular level in real time.展开更多
文摘Over the past three decades,fluorescence imaging has evolved from a descriptive tool into a precision instrument for dissecting biology at the molecular scale.1 This transformation has been propelled by parallel advances in optical instrumentation,such as super resolution micros-copy2−4 and single molecule tracking,5 and in the design of functional small molecule fluorophores.With the latest imaging technologies achieving near-molecular spatial reso-lution and submillisecond temporal precision,we are approaching the long-sought goal of interrogating biological systems at the molecular level in real time.
