A single molecule detection technique was developed by the combination of a single channel poly （dimethylsiloxane）/glass micro-fluidic chip and fluorescence correlation spectroscopy （FCS）. This method was successf...A single molecule detection technique was developed by the combination of a single channel poly （dimethylsiloxane）/glass micro-fluidic chip and fluorescence correlation spectroscopy （FCS）. This method was successfully used to determine the proportion of two model components in the mixture containing fluorescein and the rhodamine-green succinimidyl ester.展开更多
We have developed a simple method for fabricating robust and low noise glass nanopore electrodes with pore size 10±5 nm to detect single molecules.β-Cyclodextrin was used as model compound for characterization.I...We have developed a simple method for fabricating robust and low noise glass nanopore electrodes with pore size 10±5 nm to detect single molecules.β-Cyclodextrin was used as model compound for characterization.In 1.0 mol/L NaCl solution,the molecules generated current pulses of 2-5 pA with noise level less than 0.8 pA.A slide mode and a plug mode were suggested for the way ofβ-cyclodextrin single molecule moving into the glass nanopores.展开更多
Biosensors featuring single molecule detection present huge opportunities as well as challenges in food safety inspection,disease diagnosis,and environmental monitoring.Single-molecule detection is largely lacking of ...Biosensors featuring single molecule detection present huge opportunities as well as challenges in food safety inspection,disease diagnosis,and environmental monitoring.Single-molecule detection is largely lacking of high enough activity,precision molecule selectivity,and understanding in the exact operating mechanism.Single-atom catalysts(SACs),especially those metals-nitrogen-carbon that mimic the natural metalloenzyme structure,and with well-defined metal atom bond configurations,high level of molecular selectivity,and easy fabrication,endow single molecule detections with practical-use feasibilities.The recent advances in single-atom catalysts also present new pathways in the key mechanism understandings.In this short review,we will first visit the brief history and advantages of SACs that have been explored only recently for molecule-scale biosensors,where they are analogous and also differentiated from those nanozymes and natural metalloenzymes.Their applications in electrochemical,photochemical,and photoelectrochemical sensors are then discussed comprehensively by focusing on the different molecule-scale sensing modes in achieving local coordination-modulated signal amplifications.Finally,we identify new opportunities and challenges faced by these SACs-based single molecule detections in the further development of biosensors.展开更多
Advancements in single-molecule electrical detection techniques have provided a novel microscopic perspective for investigating the properties of single DNA molecules.These state-of-the-art technologies,with their ult...Advancements in single-molecule electrical detection techniques have provided a novel microscopic perspective for investigating the properties of single DNA molecules.These state-of-the-art technologies,with their ultra-high resolution at the single-event/single-base level,significantly enhance our understanding of the dynamic properties of single DNA molecules,thus providing valuable guidance for deciphering biological mechanisms,including DNA replication,repair,and transcription.展开更多
Surface plasmon resonance(SPR)sensors are based on photon-excited surface charge density oscillations confined at metal-dielectric interfaces,which makes them highly sensitive to biological or chemical molecular bindi...Surface plasmon resonance(SPR)sensors are based on photon-excited surface charge density oscillations confined at metal-dielectric interfaces,which makes them highly sensitive to biological or chemical molecular bindings to functional metallic surfaces.Metal nanostructures further concentrate surface plasmons into a smaller area than the diffraction limit,thus strengthening photon-sample interactions.However,plasmonic sensors based on intensity detection provide limited resolution with long acquisition time owing to their high vulnerability to environmental and instrumental noises.Here,we demonstrate fast and precise detection of noble gas dynamics at single molecular resolution via frequency-comb-referenced plasmonic phase spectroscopy.The photon-sample interaction was enhanced by a factor of 3,852 than the physical sample thickness owing to plasmon resonance and thermophoresis-assisted optical confinement effects.By utilizing a sharp plasmonic phase slope and a high heterodyne information carrier,a small atomic-density modulation was clearly resolved at 5 Hz with a resolution of 0.06 Ar atoms per nano-hole(in 10^(11)RIU)in Allan deviation at 0.2 s;a faster motion up to 200 Hz was clearly resolved.This fast and precise sensing technique can enable the in-depth analysis of fast fluid dynamics with the utmost resolution for a better understanding of biomedical,chemical,and physical events and interactions.展开更多
In recent years, bio-nanopore and solid-state nanopore have been greatly improved for molecule bio-sensing. Whereas, the development of this scientific field seems to have encountered a bottleneck due to their respect...In recent years, bio-nanopore and solid-state nanopore have been greatly improved for molecule bio-sensing. Whereas, the development of this scientific field seems to have encountered a bottleneck due to their respective limitations. The small pore size of the former impedes the detection of large single molecule, and the latter is difficult to achieve similar accuracy and functional control. DNA origami plays a novel role to bring more opportuni- ties for the development of nanopore technology since it is relatively easy to synthesize and modify. This review mainly focuses on introducing the DNA origami nanopore fabrication methods, characterization and application. Meanwhile, the challenges in the present DNA origami nanopore research are also discussed.展开更多
基金This work was financially supported by the National Natural Science Foundation of China. （No.20271033, 20335020, 90408014）.
文摘A single molecule detection technique was developed by the combination of a single channel poly （dimethylsiloxane）/glass micro-fluidic chip and fluorescence correlation spectroscopy （FCS）. This method was successfully used to determine the proportion of two model components in the mixture containing fluorescein and the rhodamine-green succinimidyl ester.
基金support from the National Natural Science Foundation of China(No. 20575062) The Graduate Innovation Fund of USTC
文摘We have developed a simple method for fabricating robust and low noise glass nanopore electrodes with pore size 10±5 nm to detect single molecules.β-Cyclodextrin was used as model compound for characterization.In 1.0 mol/L NaCl solution,the molecules generated current pulses of 2-5 pA with noise level less than 0.8 pA.A slide mode and a plug mode were suggested for the way ofβ-cyclodextrin single molecule moving into the glass nanopores.
基金Prof.Z.Kou acknowledges the financial support of the Fundamental Research Funds for the Central Universities(Grant No.40120631)Natural Science Foundation of Hubei Province(Grant No.2021CFB007)+2 种基金National Natural Science Foundation of China(Grant No.52202291)the support.Prof.J.Wang thanks the support by the Singapore Ministry of Education,and the National Research Foundation(NRF)research conducted at the National University of Singapore(Tier 1,A-8000186-01-00,and CRP NRF-CRP26-2021-0003).
文摘Biosensors featuring single molecule detection present huge opportunities as well as challenges in food safety inspection,disease diagnosis,and environmental monitoring.Single-molecule detection is largely lacking of high enough activity,precision molecule selectivity,and understanding in the exact operating mechanism.Single-atom catalysts(SACs),especially those metals-nitrogen-carbon that mimic the natural metalloenzyme structure,and with well-defined metal atom bond configurations,high level of molecular selectivity,and easy fabrication,endow single molecule detections with practical-use feasibilities.The recent advances in single-atom catalysts also present new pathways in the key mechanism understandings.In this short review,we will first visit the brief history and advantages of SACs that have been explored only recently for molecule-scale biosensors,where they are analogous and also differentiated from those nanozymes and natural metalloenzymes.Their applications in electrochemical,photochemical,and photoelectrochemical sensors are then discussed comprehensively by focusing on the different molecule-scale sensing modes in achieving local coordination-modulated signal amplifications.Finally,we identify new opportunities and challenges faced by these SACs-based single molecule detections in the further development of biosensors.
基金the National Key R&D Program of China(2021YFA1200102,2021YFA1200101,2023YFF1205803,2024YFA1208100,and 2022YFE0128700)the National Natural Science Foundation of China(22173050)Beijing National Laboratory for Molecular Sciences(BNLMS-CXXM-202407).
文摘Advancements in single-molecule electrical detection techniques have provided a novel microscopic perspective for investigating the properties of single DNA molecules.These state-of-the-art technologies,with their ultra-high resolution at the single-event/single-base level,significantly enhance our understanding of the dynamic properties of single DNA molecules,thus providing valuable guidance for deciphering biological mechanisms,including DNA replication,repair,and transcription.
基金supported by the National Research Foundation of the Republic of Korea(NRF-2019K1A3A1A20092429,NRF-2020R1A2C2102338,NRF-2022M1A3C2069728RS-2024-00401786)+2 种基金the Basic Research Program(NK236C)funded by the Korea Institute of Machinery and Materials(KIMM)supported by the KAIST UP Program and the Commercializations Promotion Agency for R&D Outcomes(COMPA)under grant RS-2023-00260002 and the Ministry of Small and Medium-sized Enterprises(SMEs)and Startups under grant RCMS-S3207602support of time and facilities from Ho Chi Minh City University of Technology(HCMUT),Viet Nam National University Ho Chi Minh City(VNU-HCM)。
文摘Surface plasmon resonance(SPR)sensors are based on photon-excited surface charge density oscillations confined at metal-dielectric interfaces,which makes them highly sensitive to biological or chemical molecular bindings to functional metallic surfaces.Metal nanostructures further concentrate surface plasmons into a smaller area than the diffraction limit,thus strengthening photon-sample interactions.However,plasmonic sensors based on intensity detection provide limited resolution with long acquisition time owing to their high vulnerability to environmental and instrumental noises.Here,we demonstrate fast and precise detection of noble gas dynamics at single molecular resolution via frequency-comb-referenced plasmonic phase spectroscopy.The photon-sample interaction was enhanced by a factor of 3,852 than the physical sample thickness owing to plasmon resonance and thermophoresis-assisted optical confinement effects.By utilizing a sharp plasmonic phase slope and a high heterodyne information carrier,a small atomic-density modulation was clearly resolved at 5 Hz with a resolution of 0.06 Ar atoms per nano-hole(in 10^(11)RIU)in Allan deviation at 0.2 s;a faster motion up to 200 Hz was clearly resolved.This fast and precise sensing technique can enable the in-depth analysis of fast fluid dynamics with the utmost resolution for a better understanding of biomedical,chemical,and physical events and interactions.
文摘In recent years, bio-nanopore and solid-state nanopore have been greatly improved for molecule bio-sensing. Whereas, the development of this scientific field seems to have encountered a bottleneck due to their respective limitations. The small pore size of the former impedes the detection of large single molecule, and the latter is difficult to achieve similar accuracy and functional control. DNA origami plays a novel role to bring more opportuni- ties for the development of nanopore technology since it is relatively easy to synthesize and modify. This review mainly focuses on introducing the DNA origami nanopore fabrication methods, characterization and application. Meanwhile, the challenges in the present DNA origami nanopore research are also discussed.
