Tetracentron sinense is a‘living fossil’tree in East Asia.Understanding how this‘living fossil’responds to climate change and adapts to local environments is critical for its conservation.Here,we used resequenced ...Tetracentron sinense is a‘living fossil’tree in East Asia.Understanding how this‘living fossil’responds to climate change and adapts to local environments is critical for its conservation.Here,we used resequenced genomes to clarify the evolutionary history and adaptive potential of T.sinense.We identifiedsix divergent lineages in T.sinense:three lineages from southwestern China(Yunnan Province)and three lineages from the central subtropical region of China.Additionally,we detected hybridization events between some adjacent lineages.Demographic analysis revealed that over the past 10,000 years the effective population size(Ne)of three T.sinense lineages(i.e.,NORTH,SWEST,and YNWEST)increased after their last bottleneck and then remained stable,whereas that of the remaining three lineages(i.e.,YSEAST,YC,and EAST)declined steadily.The decline in effective population size in the Yunnan lineages aligned well with the decrease in genome-wide diversity and a significantincrease in the frequency of runs of homozygosity.Deleterious variants and positively selected sites were involved in the evolution of different lineages.Further,genotype–environment association(GEA)analyses indicated adaptation to temperature-and precipitation-related factors.Genomic offset analyses found the most vulnerable populations,while SC and SC-yad were predicted to better handle extreme changes.Our findingsprovide insights into the evolutionary history and conservation of T.sinense and enhance our understanding of the evolution of living fossil species.展开更多
[Objective] This study was conducted to screen the best fertilization mode for potted Cymbidium sinense ＇Qihei＇. [Method] According to the contents of the N, P and K elements in C. sinense ＇Qihei＇ and the main fer...[Objective] This study was conducted to screen the best fertilization mode for potted Cymbidium sinense ＇Qihei＇. [Method] According to the contents of the N, P and K elements in C. sinense ＇Qihei＇ and the main fertilization modes used by producers and related literature information, the effects of 8 kinds of fertilization models on the growth of C. sinense ＇Qihei＇ were studied. Twenty six morphologic and physiological indexes of C. sinense ＇Qihei＇ growth were measured. [Result] Significant differences were shown on 21 indexes. [Conclusion] Based on the 26 in- dexes, the best fertilization mode included the steps of applying 14-14-14 （N-P20^- K20） slow-release fertilizer particles （8 g per bag） once in April, July and October, respectively; drip-applying 800 times of 30-10-10 water-soluble fertilizer once every half a month from April to September; and drip-applying 800 times of 15-5-30 wa- ter-soluble fertilizer （150 ml） once every half a month from October to December.展开更多
[Objective] The aim was to clone Syntaxin genes in Limonium sinense Kuntze. [Method] Limonium sinense Kuntze leaves were used as materials and total RNA was extracted and transcribed reversely. Nested primers were des...[Objective] The aim was to clone Syntaxin genes in Limonium sinense Kuntze. [Method] Limonium sinense Kuntze leaves were used as materials and total RNA was extracted and transcribed reversely. Nested primers were designed based on EST sequences at 5’ region of Syntaxin, and cDNA obtained through reverse reaction was taken as the template. Sequences of Syntaxin gene at 3’ region were obtained through two rounds of PCR amplifications. [Result] DNA fragments （1 096 bp） were obtained. For LsSyntaxin, open reading frame （ORF） was 816 bp and the encoded amino acids were 271. The relative molecular weight of Syntaxin was 30 254.3 Da and isoelectric point in theory was 5.55. [Conclusion] Syntaxin genes from Limonium sinense Kuntze were cloned. The research laid foundation for the study on Syntaxin gene function in Limonium sinense Kuntze and salt-secreted process.展开更多
To analyze the degree and pattern of phenotypic variation in leaves of Tetracentron sinense Oliv from the perspective of genetic and environmental adaptation and thus contribute to effective evidence-based conservatio...To analyze the degree and pattern of phenotypic variation in leaves of Tetracentron sinense Oliv from the perspective of genetic and environmental adaptation and thus contribute to effective evidence-based conservation and management strategies for germplasm resources,we measured 17 morphological and epidermal micromorphological leaf traits from 24 natural populations of T.sinense.Nested analysis of variance,multiple comparison,principal component analysis(PCA),cluster analysis,and correlation analysis were used to explore phenotypic leaf variation among and within populations and potential correlations with geographic and environmental factors.There were significant differences in 17 leaf phenotypic traits among and within populations.The mean phenotypic differentiation coefficient of the 17 traits was 56.34%,and the variation among populations(36.4%)was greater than that within populations(27.2%).The coefficient of variation(CV)of each trait ranged from 4.6 to 23.8%,and the mean was 11.8%.Phenotypic variation of leaves was related to environmental factors such as average annual sunshine hours,average July temperature,and average annual rainfall.The variation changed along gradients of longitude,latitude,and altitude.The PCA clustered the 24 natural populations into four groups.Our study suggests that phenotypic variation in T.sinense occurred primarily among populations,with moderate levels of phenotypic differentiation among populations and low levels of phenotypic variation within populations.The plant’s poor adaptability to the environment is likely an important contributor to its endangerment.Accordingly,conservation strategies are proposed to protect and manage the natural populations of T.sinense.展开更多
A new alkaloid,sinensine(1),had been isolated from the fruiting bodies of Ganoderma sinense Zhao,Xu et Zhang.Its structure was elucidated on the basis of 1D and 2D spectral analysis.This alkaloid exhibited activity in...A new alkaloid,sinensine(1),had been isolated from the fruiting bodies of Ganoderma sinense Zhao,Xu et Zhang.Its structure was elucidated on the basis of 1D and 2D spectral analysis.This alkaloid exhibited activity in protecting the injury induced by hydrogen peroxide oxidation on HUVEC,with EC_(50) value 6.2μmol/L.展开更多
Four new alkaloids,sinensines B-E(1-4),together with one known alkaloid,sinensine(5),were isolated from the fruiting bodies of Ganoderma sinense.Their structures were elucidated on the basis of 1D and 2D NMR spectra a...Four new alkaloids,sinensines B-E(1-4),together with one known alkaloid,sinensine(5),were isolated from the fruiting bodies of Ganoderma sinense.Their structures were elucidated on the basis of 1D and 2D NMR spectra analysis.The structure of sinensine E was confirmed by X-ray crystallographic analysis of its acetyl product(4a).展开更多
The fast growth of Tetracentron sinense is a potential valuable timber resource, but whether its anatomy and chemical components are suitable for timber is unknown. We used light microscopy and SEM to examine the anat...The fast growth of Tetracentron sinense is a potential valuable timber resource, but whether its anatomy and chemical components are suitable for timber is unknown. We used light microscopy and SEM to examine the anatomical structure and FITR to measure the chemical components of the phloem and xylem of this tree. Radial variations in growth ring width and tracheid dimensions were also evaluated. The sieve tube, phloem parenchyma cell and sclereids clusters were the main cells in phloem, and the tracheid was the fundamental cell in xylem. An unusual tracheid type, fiber-tracheids or vessel-liked elements was visible. Wood rays nonstoried, uniseriate and multiseriate, including heterogeneous II, occasionally I, and usually 3-6 cells wide. The mean growth-ring width was 2.53 +/- 0.46 mm, and the percentage of late wood was over 60%. For radial variation, growth-ring width increased at an early growth stage, and reached the largest increment during years 11-15, then decreased. The maximum growth-ring width was 5.313 mm. During late growth (60-85 years), trees also maintained a high radial growth increment. Radial variation in the percentage of late wood was uniform, about 50-70%, throughout the growth years. Growth patterns in the length and width of early and late wood were similar as the trees aged. From the FTIR results, the chemical components differed significantly between xylem and phloem, hemicellulose in particular was higher in the xylem than in the phloem, where it was apparently absent. All of these suggest that the composition of phloem in T. sinense is very similar to that of hardwood, and it has higher growth ratio and uniform wood properties.展开更多
The Structural nature of Tctracctron sinense MWL is characterized by means of Chemical analysis.GC,IR,’H NMR and <sup>C</sup>（13） NMR spectra.The researches indicated that the MWL is of guaiacyl—syring...The Structural nature of Tctracctron sinense MWL is characterized by means of Chemical analysis.GC,IR,’H NMR and <sup>C</sup>（13） NMR spectra.The researches indicated that the MWL is of guaiacyl—syringyl type.The main connective form between structural units is β—O—4aryl ether bonds.The quantity of β—O—4 and 5—5 substructures is 0.53/C<sub>9</sub> and 0.06/C<sub>9</sub>,respectively.The alipatic and phenolic hydroxy contents are respectively.The OCH<sub>3</sub> content is estimated by <sup>C</sup>（13）NMR is spectrum （1.14/C<sub>9</sub>）,which is con-sistent with chemical method（1.11/C<sub>9</sub>） The degree of condensetion of the MWL is 0.51/C<sub>9</sub>（from ’HNMR）,which is consistent with the result from <sup>C</sup>（13）NMR.展开更多
The regeneration of Tetracentron sinense Oliv.is poor in the understory and in open areas due to the charac-teristics of natural regeneration of the species on forest edges and in gaps.It is unclear whether different ...The regeneration of Tetracentron sinense Oliv.is poor in the understory and in open areas due to the charac-teristics of natural regeneration of the species on forest edges and in gaps.It is unclear whether different light intensities in various habitats affect eco-physiological characteristics of saplings and their natural regeneration.In this study,the light intensity in T.sinense habitats was simulated by artificial shading(L1:100%NS(natural sunlight)in the open;L2:50%NS in a forest gap or edge;L3:10%NS in understory)to investigate differences in morphology,leaf structure,physiology,and photosynthesis of 2-year-old sap-lings,and to analyze the mechanism of light intensity on sapling establishment.Significant differences were observed in morphology(including leaf area,and specific leaf area)under different light intensities.Compared to L1 and L3,chloroplast structure in L2 was intact.With increasing time,superoxide dismutase(SOD)and catalase(CAT)activities in L2 became gradually higher than under the other light intensities,while malondialdehyde(MDA)content was opposite.Shading decreased osmoregulation substance contents of leaves but increased chlorophyll.The results suggest that light intensities significantly affect the eco-physiological characteristics of T.sinense saplings and they would respond most favorably at intermediate levels of light by optimizing eco-physiological characteristics.Therefore,50%natural sunlight should be created to promote saplings establishment and population recovery of T.sinense during in situ conservation,including sowing mature seeds in forest edges or gaps and providing appropriate shade protection for seedlings and saplings in the open.展开更多
The wood powder of Tetracentron Sinense Olive was extracted with the benzene and alcohol (2:1 v/v). Then, the extractive is analyzed in Gc and Gc-Ms.Twenty chemical constitutions of the extractive are separated and id...The wood powder of Tetracentron Sinense Olive was extracted with the benzene and alcohol (2:1 v/v). Then, the extractive is analyzed in Gc and Gc-Ms.Twenty chemical constitutions of the extractive are separated and identified, which are as follows: calamenene; 2. 6,10-trimethyldihexyl; 3 -methoxybenzaldehyde; Methyl- 4- methox- ybenzoate; 4- methoxyphenylacetone; 3. 4- dimethoxybenzaldehyde;. 3. 4 -dimethoxyhypnone; methyl-3. 4-dimethoxybenzoate, Methyl-4-methoxyphenylacrylate,4,4-dimethoxycabonyl-diphenylethane; Methyl-3, 4,5 - erimethoxybenzoate; Methyl - 3. 4 -dimethoxyphenylacrylate; Methyl-myristate; Methyl - palmate, Methyl- zoomarate;Methyl-stearate; Methyl-oleate; Methyl-arachidate; Methyl-behenate, Dimethyl -azelate.展开更多
Ligusticum sinense cv.Chuanxiong(L.Chuanxiong),one of the widely used traditional Chinese medicines(TCM),is currently facing the problem of excessive cadmium(Cd)content.This problem has significantly affected the qual...Ligusticum sinense cv.Chuanxiong(L.Chuanxiong),one of the widely used traditional Chinese medicines(TCM),is currently facing the problem of excessive cadmium(Cd)content.This problem has significantly affected the quality and safety of L.Chuanxiong and become a vital factor restricting its clinical application and international trade development.Currently,to solve the problem of excessive Cd,it is essential to research the response mechanisms of L.Chuanxiong to Cd stress.However,there are few reports on its physiological and biochemical responses under Cd stress.In this study,we conducted the hydroponic experiment under 25μM Cd stress,based on the Cd content of the genuine producing areas soil.The results showed that 25μM Cd stress not only had no significant inhibitory effect on the growth of L.Chuanxiong seedlings but also significantly increased the chlorophyll a content(11.79%)and root activity(51.82%)compared with that of the control,which might be a hormesis effect.Further results showed that the absorption and assimilation of NH_(4)^(+)increased in seedlings under 25μM Cd stress,which was associated with high photosynthetic pigments.Here,we initially hypothesized and confirmed that Cd exceedance in the root system of L.Chuanxiong was due to the thickening of the root cell wall,changes in the content of the cell wall components,and chelation of Cd by GSH.There was an increase in cell wall thickness(57.64%)and a significant increase in cellulose(25.48%)content of roots under 25μM Cd stress.In addition,L.Chuanxiong reduced oxidative stress caused by 25μM Cd stress mainly through the GSH/GSSG cycle.Among them,GSH-Px(48.26%)and GR(42.64%)activities were significantly increased,thereby maintaining a high GSH/GSSG ratio.This study preliminarily reveals the response of L.Chuanxiong to Cd stress and the mechanism of Cd enrichment.It provides a theoretical basis for solving the problem of Cd excessive in L.Chuanxiong.展开更多
A novel alkaloid,named hypodematine,was isolated from the whole plant of a fern.Htpodematium sinemse lwatsuki.Its structure has been elucidated to have a novel skeleton of benzo-azacyclooctatetraene with a phenyl subs...A novel alkaloid,named hypodematine,was isolated from the whole plant of a fern.Htpodematium sinemse lwatsuki.Its structure has been elucidated to have a novel skeleton of benzo-azacyclooctatetraene with a phenyl substituent by means of ~1H-~1H COSY.~1H-(13)C COSY.and long-range ~1H-^(13)C COSY spectroscopy.展开更多
基金supported by the National Natural Science Foundation of China(no.32570426)the Key Basic Research Program of Yunnan Province,China(202101BC070003)+1 种基金the Fundamental Research Funds for the Central Universities(QNTD202502)the STI 2030—Major Program(2022ZD0401605-2).
文摘Tetracentron sinense is a‘living fossil’tree in East Asia.Understanding how this‘living fossil’responds to climate change and adapts to local environments is critical for its conservation.Here,we used resequenced genomes to clarify the evolutionary history and adaptive potential of T.sinense.We identifiedsix divergent lineages in T.sinense:three lineages from southwestern China(Yunnan Province)and three lineages from the central subtropical region of China.Additionally,we detected hybridization events between some adjacent lineages.Demographic analysis revealed that over the past 10,000 years the effective population size(Ne)of three T.sinense lineages(i.e.,NORTH,SWEST,and YNWEST)increased after their last bottleneck and then remained stable,whereas that of the remaining three lineages(i.e.,YSEAST,YC,and EAST)declined steadily.The decline in effective population size in the Yunnan lineages aligned well with the decrease in genome-wide diversity and a significantincrease in the frequency of runs of homozygosity.Deleterious variants and positively selected sites were involved in the evolution of different lineages.Further,genotype–environment association(GEA)analyses indicated adaptation to temperature-and precipitation-related factors.Genomic offset analyses found the most vulnerable populations,while SC and SC-yad were predicted to better handle extreme changes.Our findingsprovide insights into the evolutionary history and conservation of T.sinense and enhance our understanding of the evolution of living fossil species.
基金Supported by Science and Technology Planning Project of Guangdong Province(2012A020602036)
文摘[Objective] This study was conducted to screen the best fertilization mode for potted Cymbidium sinense ＇Qihei＇. [Method] According to the contents of the N, P and K elements in C. sinense ＇Qihei＇ and the main fertilization modes used by producers and related literature information, the effects of 8 kinds of fertilization models on the growth of C. sinense ＇Qihei＇ were studied. Twenty six morphologic and physiological indexes of C. sinense ＇Qihei＇ growth were measured. [Result] Significant differences were shown on 21 indexes. [Conclusion] Based on the 26 in- dexes, the best fertilization mode included the steps of applying 14-14-14 （N-P20^- K20） slow-release fertilizer particles （8 g per bag） once in April, July and October, respectively; drip-applying 800 times of 30-10-10 water-soluble fertilizer once every half a month from April to September; and drip-applying 800 times of 15-5-30 wa- ter-soluble fertilizer （150 ml） once every half a month from October to December.
基金Supported by National Natural Science Foundation of China(30870199)Shandong Natural Science Foundation(Y2007D34+1 种基金ZR2011CM006)Key Projects of Shandong Natural Science Foundation(2010GNC10937)~~
文摘[Objective] The aim was to clone Syntaxin genes in Limonium sinense Kuntze. [Method] Limonium sinense Kuntze leaves were used as materials and total RNA was extracted and transcribed reversely. Nested primers were designed based on EST sequences at 5’ region of Syntaxin, and cDNA obtained through reverse reaction was taken as the template. Sequences of Syntaxin gene at 3’ region were obtained through two rounds of PCR amplifications. [Result] DNA fragments （1 096 bp） were obtained. For LsSyntaxin, open reading frame （ORF） was 816 bp and the encoded amino acids were 271. The relative molecular weight of Syntaxin was 30 254.3 Da and isoelectric point in theory was 5.55. [Conclusion] Syntaxin genes from Limonium sinense Kuntze were cloned. The research laid foundation for the study on Syntaxin gene function in Limonium sinense Kuntze and salt-secreted process.
基金funded by the Sichuan Science and Technology Program,Sichuan Province,China(No.2017JY0164)the Special Fund for the Fourth General Survey of Chinese Medicine Resources in China(No.2018PC001)+1 种基金Meritocracy Research Funds of China West Normal University(No.17YC325)the Fundamental Research Funds of China West Normal University(No.18B026).
文摘To analyze the degree and pattern of phenotypic variation in leaves of Tetracentron sinense Oliv from the perspective of genetic and environmental adaptation and thus contribute to effective evidence-based conservation and management strategies for germplasm resources,we measured 17 morphological and epidermal micromorphological leaf traits from 24 natural populations of T.sinense.Nested analysis of variance,multiple comparison,principal component analysis(PCA),cluster analysis,and correlation analysis were used to explore phenotypic leaf variation among and within populations and potential correlations with geographic and environmental factors.There were significant differences in 17 leaf phenotypic traits among and within populations.The mean phenotypic differentiation coefficient of the 17 traits was 56.34%,and the variation among populations(36.4%)was greater than that within populations(27.2%).The coefficient of variation(CV)of each trait ranged from 4.6 to 23.8%,and the mean was 11.8%.Phenotypic variation of leaves was related to environmental factors such as average annual sunshine hours,average July temperature,and average annual rainfall.The variation changed along gradients of longitude,latitude,and altitude.The PCA clustered the 24 natural populations into four groups.Our study suggests that phenotypic variation in T.sinense occurred primarily among populations,with moderate levels of phenotypic differentiation among populations and low levels of phenotypic variation within populations.The plant’s poor adaptability to the environment is likely an important contributor to its endangerment.Accordingly,conservation strategies are proposed to protect and manage the natural populations of T.sinense.
基金supported by the Ministry of Science and Technology of the People's Republic of China (No.2007Fy130100)
文摘A new alkaloid,sinensine(1),had been isolated from the fruiting bodies of Ganoderma sinense Zhao,Xu et Zhang.Its structure was elucidated on the basis of 1D and 2D spectral analysis.This alkaloid exhibited activity in protecting the injury induced by hydrogen peroxide oxidation on HUVEC,with EC_(50) value 6.2μmol/L.
基金The project was financially supported by the General Program of NSFC(No.81172940)Knowledge Innovation Program of the CAS(Grant No.KSCX2-YW-G-038,KSCX2-YW-R-194,KZCX2-XB2-15-03,)as well as Foundation of State Key Laboratory of Phytochemistry and Plant Resources in West China(P2010-ZZ14).
文摘Four new alkaloids,sinensines B-E(1-4),together with one known alkaloid,sinensine(5),were isolated from the fruiting bodies of Ganoderma sinense.Their structures were elucidated on the basis of 1D and 2D NMR spectra analysis.The structure of sinensine E was confirmed by X-ray crystallographic analysis of its acetyl product(4a).
基金financially supported by the Youth Science and Technology Innovation Fund of NJFU(CXL2015018)the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)
文摘The fast growth of Tetracentron sinense is a potential valuable timber resource, but whether its anatomy and chemical components are suitable for timber is unknown. We used light microscopy and SEM to examine the anatomical structure and FITR to measure the chemical components of the phloem and xylem of this tree. Radial variations in growth ring width and tracheid dimensions were also evaluated. The sieve tube, phloem parenchyma cell and sclereids clusters were the main cells in phloem, and the tracheid was the fundamental cell in xylem. An unusual tracheid type, fiber-tracheids or vessel-liked elements was visible. Wood rays nonstoried, uniseriate and multiseriate, including heterogeneous II, occasionally I, and usually 3-6 cells wide. The mean growth-ring width was 2.53 +/- 0.46 mm, and the percentage of late wood was over 60%. For radial variation, growth-ring width increased at an early growth stage, and reached the largest increment during years 11-15, then decreased. The maximum growth-ring width was 5.313 mm. During late growth (60-85 years), trees also maintained a high radial growth increment. Radial variation in the percentage of late wood was uniform, about 50-70%, throughout the growth years. Growth patterns in the length and width of early and late wood were similar as the trees aged. From the FTIR results, the chemical components differed significantly between xylem and phloem, hemicellulose in particular was higher in the xylem than in the phloem, where it was apparently absent. All of these suggest that the composition of phloem in T. sinense is very similar to that of hardwood, and it has higher growth ratio and uniform wood properties.
文摘The Structural nature of Tctracctron sinense MWL is characterized by means of Chemical analysis.GC,IR,’H NMR and <sup>C</sup>（13） NMR spectra.The researches indicated that the MWL is of guaiacyl—syringyl type.The main connective form between structural units is β—O—4aryl ether bonds.The quantity of β—O—4 and 5—5 substructures is 0.53/C<sub>9</sub> and 0.06/C<sub>9</sub>,respectively.The alipatic and phenolic hydroxy contents are respectively.The OCH<sub>3</sub> content is estimated by <sup>C</sup>（13）NMR is spectrum （1.14/C<sub>9</sub>）,which is con-sistent with chemical method（1.11/C<sub>9</sub>） The degree of condensetion of the MWL is 0.51/C<sub>9</sub>（from ’HNMR）,which is consistent with the result from <sup>C</sup>（13）NMR.
基金funded by the National Natural Science Foundation of China (No.32070371)the Innovation Team Funds of China West Normal University (No.KCXTD2022-4)+1 种基金the fund of Sichuan Meigu Dafegnding National Nature Reserve (No.mgdfd2022-13)Sichuan Micang Mountain National Nature Reserve (No.N5108212022000043)。
文摘The regeneration of Tetracentron sinense Oliv.is poor in the understory and in open areas due to the charac-teristics of natural regeneration of the species on forest edges and in gaps.It is unclear whether different light intensities in various habitats affect eco-physiological characteristics of saplings and their natural regeneration.In this study,the light intensity in T.sinense habitats was simulated by artificial shading(L1:100%NS(natural sunlight)in the open;L2:50%NS in a forest gap or edge;L3:10%NS in understory)to investigate differences in morphology,leaf structure,physiology,and photosynthesis of 2-year-old sap-lings,and to analyze the mechanism of light intensity on sapling establishment.Significant differences were observed in morphology(including leaf area,and specific leaf area)under different light intensities.Compared to L1 and L3,chloroplast structure in L2 was intact.With increasing time,superoxide dismutase(SOD)and catalase(CAT)activities in L2 became gradually higher than under the other light intensities,while malondialdehyde(MDA)content was opposite.Shading decreased osmoregulation substance contents of leaves but increased chlorophyll.The results suggest that light intensities significantly affect the eco-physiological characteristics of T.sinense saplings and they would respond most favorably at intermediate levels of light by optimizing eco-physiological characteristics.Therefore,50%natural sunlight should be created to promote saplings establishment and population recovery of T.sinense during in situ conservation,including sowing mature seeds in forest edges or gaps and providing appropriate shade protection for seedlings and saplings in the open.
文摘The wood powder of Tetracentron Sinense Olive was extracted with the benzene and alcohol (2:1 v/v). Then, the extractive is analyzed in Gc and Gc-Ms.Twenty chemical constitutions of the extractive are separated and identified, which are as follows: calamenene; 2. 6,10-trimethyldihexyl; 3 -methoxybenzaldehyde; Methyl- 4- methox- ybenzoate; 4- methoxyphenylacetone; 3. 4- dimethoxybenzaldehyde;. 3. 4 -dimethoxyhypnone; methyl-3. 4-dimethoxybenzoate, Methyl-4-methoxyphenylacrylate,4,4-dimethoxycabonyl-diphenylethane; Methyl-3, 4,5 - erimethoxybenzoate; Methyl - 3. 4 -dimethoxyphenylacrylate; Methyl-myristate; Methyl - palmate, Methyl- zoomarate;Methyl-stearate; Methyl-oleate; Methyl-arachidate; Methyl-behenate, Dimethyl -azelate.
基金supported by Natural Science Foundation of Sichuan Province(2024NSFSC1832)The National Natural Science Foundation of China(grant numbers 82404796,82373986)+2 种基金China Postdoctoral Science Foundation(grant number 2023M730381)Key R&D Projects of Sichuan Provincial Department of Science and Technology(grant numbers 22ZDYF1861)Natural Science Foundation of Sichuan Province(2024NSFSC1831).
文摘Ligusticum sinense cv.Chuanxiong(L.Chuanxiong),one of the widely used traditional Chinese medicines(TCM),is currently facing the problem of excessive cadmium(Cd)content.This problem has significantly affected the quality and safety of L.Chuanxiong and become a vital factor restricting its clinical application and international trade development.Currently,to solve the problem of excessive Cd,it is essential to research the response mechanisms of L.Chuanxiong to Cd stress.However,there are few reports on its physiological and biochemical responses under Cd stress.In this study,we conducted the hydroponic experiment under 25μM Cd stress,based on the Cd content of the genuine producing areas soil.The results showed that 25μM Cd stress not only had no significant inhibitory effect on the growth of L.Chuanxiong seedlings but also significantly increased the chlorophyll a content(11.79%)and root activity(51.82%)compared with that of the control,which might be a hormesis effect.Further results showed that the absorption and assimilation of NH_(4)^(+)increased in seedlings under 25μM Cd stress,which was associated with high photosynthetic pigments.Here,we initially hypothesized and confirmed that Cd exceedance in the root system of L.Chuanxiong was due to the thickening of the root cell wall,changes in the content of the cell wall components,and chelation of Cd by GSH.There was an increase in cell wall thickness(57.64%)and a significant increase in cellulose(25.48%)content of roots under 25μM Cd stress.In addition,L.Chuanxiong reduced oxidative stress caused by 25μM Cd stress mainly through the GSH/GSSG cycle.Among them,GSH-Px(48.26%)and GR(42.64%)activities were significantly increased,thereby maintaining a high GSH/GSSG ratio.This study preliminarily reveals the response of L.Chuanxiong to Cd stress and the mechanism of Cd enrichment.It provides a theoretical basis for solving the problem of Cd excessive in L.Chuanxiong.
文摘A novel alkaloid,named hypodematine,was isolated from the whole plant of a fern.Htpodematium sinemse lwatsuki.Its structure has been elucidated to have a novel skeleton of benzo-azacyclooctatetraene with a phenyl substituent by means of ~1H-~1H COSY.~1H-(13)C COSY.and long-range ~1H-^(13)C COSY spectroscopy.
