To effective prevent and control the disease of Penaeus vannamei Boone in Hainan Province, the morphology, cultural characteristics, physiological and biochemical reactions of the dominant strain isolated from the hep...To effective prevent and control the disease of Penaeus vannamei Boone in Hainan Province, the morphology, cultural characteristics, physiological and biochemical reactions of the dominant strain isolated from the hepatopanereas tissue of diseased P. vannamei were identified by conventional metheds, and 16S rDNA sequence was amplified by polymerase chain reaction （PCR） for sequencing analysis. Furthermore, artificial experimental infection was conducted to de- tect the pathogenicity of the isolate. Finally, the lytic ability of phage 28TM against the isolate was detected by in-vitro lysis test. The results showed that the isolate （HYRJ2013） was identified as Vibrio sinaloensis. There were similar symptoms with the natural incidence of P. vannamei when the isolate HYRJ2013 was used to infect P. vannamei by muscular injection. The median lethal dose （ LD50 ） was measured as 6.68 x 107 cfu/ml. Phage 28TM could lyse the isolate and be used for biological control. The results indicate that V. sinaloensis is an opportunistic pathogen to P. vannamei.展开更多
基金Supported by the Colleges and Universities Scientific Research Projects of the Education Department of Nation(201311810046)Health Department Project of Hainan Province(QW2013 ZU-053)the Colleges and Universities Scientific Research Projects of the Education Department of Hainan Medical University(HYCX201213)
文摘To effective prevent and control the disease of Penaeus vannamei Boone in Hainan Province, the morphology, cultural characteristics, physiological and biochemical reactions of the dominant strain isolated from the hepatopanereas tissue of diseased P. vannamei were identified by conventional metheds, and 16S rDNA sequence was amplified by polymerase chain reaction （PCR） for sequencing analysis. Furthermore, artificial experimental infection was conducted to de- tect the pathogenicity of the isolate. Finally, the lytic ability of phage 28TM against the isolate was detected by in-vitro lysis test. The results showed that the isolate （HYRJ2013） was identified as Vibrio sinaloensis. There were similar symptoms with the natural incidence of P. vannamei when the isolate HYRJ2013 was used to infect P. vannamei by muscular injection. The median lethal dose （ LD50 ） was measured as 6.68 x 107 cfu/ml. Phage 28TM could lyse the isolate and be used for biological control. The results indicate that V. sinaloensis is an opportunistic pathogen to P. vannamei.
