Rattan is a typical tropical climbing plant that uses flagella to climb supports to grow.A comprehensive understanding of the anatomic structure and micromechanics of rattan flagella might inspire more research on bio...Rattan is a typical tropical climbing plant that uses flagella to climb supports to grow.A comprehensive understanding of the anatomic structure and micromechanics of rattan flagella might inspire more research on biomimetic climbing materials.Here,the structure and micromechanical properties of flagella in calamus simplicifolius were examined by Micro-Computed Tomography(Micro-CT)and nanoindentation techniques,respectively.The results showed that the rachis of the flagella mainly comprised vascular bundles surrounded by basic tissues,which had a gradient density decreasing from outsides to insides.The prickles are derived from the epidermis or the epidermis and cortical tissue of the flagellum,which do not possess vascular tissue.The entire tip of the prickle was composed almost of fibrous cells.The indentation modulus of elasticity of the prickle was 17.03 GPa,which was 17.93%higher in comparison with the rachis.The hardness of the prickle was 539.27 MPa and was slightly higher than that of the rachis.The results indicated that the discrepancy of micromechanical strengths in different parts of flagella reflects on their unique roles in the process of climbing.展开更多
The random amplified polymorphic DNA （RAPD） molecular marker technique was used to determine the sex of Calamus simplicifolius C. F. Wei In the present study, DNA samples were extracted individually from 10 male and...The random amplified polymorphic DNA （RAPD） molecular marker technique was used to determine the sex of Calamus simplicifolius C. F. Wei In the present study, DNA samples were extracted individually from 10 male and 10 female plants. After a total of 1 040 decamer primers had been tested, an approximate 500-bp male-specific DNA fragment was generated with the S 1443 primer. It is feasible to identify sex at the early stages of plant life, which is beneficial for improving breeding programs of this dioecious species. In addition, we have obtained a proper RAPD protocol that is useful for other species of rattan.展开更多
基金Basic Scientific Research Funds of the National Natural Science Foundation(Grant No.31800476)International Center for Bamboo and Rattan(Grant No.1632020011).
文摘Rattan is a typical tropical climbing plant that uses flagella to climb supports to grow.A comprehensive understanding of the anatomic structure and micromechanics of rattan flagella might inspire more research on biomimetic climbing materials.Here,the structure and micromechanical properties of flagella in calamus simplicifolius were examined by Micro-Computed Tomography(Micro-CT)and nanoindentation techniques,respectively.The results showed that the rachis of the flagella mainly comprised vascular bundles surrounded by basic tissues,which had a gradient density decreasing from outsides to insides.The prickles are derived from the epidermis or the epidermis and cortical tissue of the flagellum,which do not possess vascular tissue.The entire tip of the prickle was composed almost of fibrous cells.The indentation modulus of elasticity of the prickle was 17.03 GPa,which was 17.93%higher in comparison with the rachis.The hardness of the prickle was 539.27 MPa and was slightly higher than that of the rachis.The results indicated that the discrepancy of micromechanical strengths in different parts of flagella reflects on their unique roles in the process of climbing.
文摘The random amplified polymorphic DNA （RAPD） molecular marker technique was used to determine the sex of Calamus simplicifolius C. F. Wei In the present study, DNA samples were extracted individually from 10 male and 10 female plants. After a total of 1 040 decamer primers had been tested, an approximate 500-bp male-specific DNA fragment was generated with the S 1443 primer. It is feasible to identify sex at the early stages of plant life, which is beneficial for improving breeding programs of this dioecious species. In addition, we have obtained a proper RAPD protocol that is useful for other species of rattan.
