As an important ecological tree species in northern China, Populus simonii plays a crucial role in maintaining ecological balance and promoting environmental sustainability. The academic community has conducted a seri...As an important ecological tree species in northern China, Populus simonii plays a crucial role in maintaining ecological balance and promoting environmental sustainability. The academic community has conducted a series of in-depth studies on this species, covering key areas such as genomics, survival mechanisms, and genetic breeding. Through the analysis of the genomic structure and function of P. simonii, we have not only revealed the molecular basis for its adaptation to harsh environments but also identified key genes that promote its growth and resistance to pests and diseases. Furthermore, exploring the survival mechanisms of P. simonii has deepened our understanding of its stress resistance traits, including how it effectively copes with abiotic stresses such as drought, salinization, and heavy metal pollution. In genetic breeding, significant progress has been made through the application of modern biotechnology, improving the growth rate and wood quality of P. simonii and enhancing its environmental adaptability and disease resistance. These research findings have not only enriched our knowledge of the biological characteristics of P. simonii but also provided a solid scientific foundation for its application in ecological restoration, forestry production, and environmental management.展开更多
The role of plant eIF5A proteins in multiple biological processes, such as protein synthesis regulation, translation elongation, mRNA turnover, programmed cell death and stress tolerance is well known. Toward using th...The role of plant eIF5A proteins in multiple biological processes, such as protein synthesis regulation, translation elongation, mRNA turnover, programmed cell death and stress tolerance is well known. Toward using these powerful proteins to increase stress tolerance in agricultural plants, in the present study, we cloned and characterized PsneIFSA2 and PsneIFSA4 from young poplar （P. simonii × P. nigra） leaves. The deduced amino acid sequences of PsneIF5A2 and PsneIF5A4 were 98 % similar to each other, and they are orthologs of eIF5A 1 in Arabidopsis. In a subcellular localization analysis, PsneIF5A2 and PsneIF5A4 proteins were localized in the nucleus and cytoplasm, qRT-PCR analysis showed that PsneIF5A2 and PsneIF5A4 were transcribed in poplar flowers, stem, leaves, and roots. In addition, they were also induced by abiotic stresses. Transgenic yeast expressing PsneIF5A2 and PsneIF5A4 had increased salt, heavy metal, osmotic, oxidative tolerance. Our results suggest that PsneIF5A2 and PsneIF5A4 are excellent candidates for genetic engineering to improve salt and heavy metal tolerance in agricultural plants.展开更多
We investigated phenotypic variation ofPopulus simonii from five provenances in northern China. Our results show that significant differences exist between plants of different provenances in morphological and physiolo...We investigated phenotypic variation ofPopulus simonii from five provenances in northern China. Our results show that significant differences exist between plants of different provenances in morphological and physiological traits, except for leaf-stalk length and the number of leaf margins. The coefficient of variation for all traits ranges from 14.77% to 81.49%. The mean phenotypic coefficient of differentiation （VST） is 47.1%, which means that the variation within provenances is the major source for phenotypic variation in P. simonii. Given our cluster analysis of provenances based on an average linkage computing method, the five provenances ofP. simonii investigated could be divided into three groups. Our results provide a theoretical basis for genetic resource conservation and provenance selection of natural P. simonii in northern China.展开更多
Aldehydes play an important role in atmospheric chemistry and plant direct and indirect defense against environmental stresses.In this study,the emissions of saturated C6-C10 aldehydes from Populus simonii × P.py...Aldehydes play an important role in atmospheric chemistry and plant direct and indirect defense against environmental stresses.In this study,the emissions of saturated C6-C10 aldehydes from Populus simonii × P.pyramidalis ＇Opera 8277＇ cuttings were examined by using a gas chromatography/mass spectrometry（GC/MS） technique at three levels of light intensity（400,800 and 1 200 μmol·m-2·s-1）.A positive correlation between the emissions of these aldehydes and light intensity was found.Moreover,nordi-hydroguaiaretic acid（NDGA）,a special inhibitor of lipoxygenase（LOX）,significantly inhibited the emissions of C6-C9 aldehydes at three levels of light intensity,but did not influence the emission of decanal（C10）.The emissions of C6-C10 aldehydes in NDGA treated poplar cuttings,exhibited the same positive correlation with light intensity.The results indicated that LOX pathway contributes to the emissions of C6-C9 aldehydes,whereas some pathways regulated by light intensity might be a universal mechanism for emissions of C6-C10 aldehydes.展开更多
[ Objective ] This study aimed to analyze the functions of AP1 gene from Populus simonii × Populus nigra and to lay the theoretical foundation for shortening the breeding cycle of forest trees and investigating t...[ Objective ] This study aimed to analyze the functions of AP1 gene from Populus simonii × Populus nigra and to lay the theoretical foundation for shortening the breeding cycle of forest trees and investigating the flowering mechanism in poplar. [ Method] Plant expression vectors of AP1 genes were constructed and transformed into tobacco leaf disks with Agrobacterium-mediated method. Transgenic tobacco plants were identified by PCR. [ Result] AP1 genes were integrated into the genome of tobacco. Transgenic tobacco plants all presented an early flowering phenotype compared with wild-type tobacco. [ Conclusion] AP1 genes could promote early flowering in transgenic tobacco plants, which provided theoretical basis for molecular regulation of flowering in poplar.展开更多
Populus simonii Carr., one of the main poplar tree species, is cultivated widely in Northeast and Northwest China in protection and timber forests. Plant phenology plays an important role in timber production by contr...Populus simonii Carr., one of the main poplar tree species, is cultivated widely in Northeast and Northwest China in protection and timber forests. Plant phenology plays an important role in timber production by controlling the growing period (i.e., the period between the leaf unfolding and the leaf turning yellow). It is important to understand this control mechanism and to improve the accuracy of the simulation of leaf unfolding phenology for P. simonii in order to determine accurately the timber production of P. simonii plantations. In this study, based on phenological observation data from 10 agricultural meteorological stations in Heilongjiang Province, China, model simulation was employed to determine the control mechanism of leaf unfolding of P. simonii. Furthermore, the predicting effects of nine phenology-simulating models for P. simonii leaf unfolding were evaluated and the distribution characteristics of P. simonii leaf unfolding in China in 2015 were simulated. The results show that P. simonii leaf unfolding is sensitive to air temperature;consequently, climate warming could advance the P. simonii leaf unfolding process. The phenological model based on air temperature could be better suited for simulating P. simonii leaf unfolding, with 76.7% of the calibration data of absolute error being less than three days. The performance of the models based solely on forcing requirements was found superior to that of the models incorporating chilling. If it was imperative that the chilling threshold is reached, the south of the Yunnan, Guangdong, and Guangxi provinces would be unsuitable for planting P. simonii. In this regard, the phenology model based on the chilling threshold as necessary condition was indicated a more reasonable model for the distribution characteristics of P. simonii leaf unfolding.展开更多
文摘As an important ecological tree species in northern China, Populus simonii plays a crucial role in maintaining ecological balance and promoting environmental sustainability. The academic community has conducted a series of in-depth studies on this species, covering key areas such as genomics, survival mechanisms, and genetic breeding. Through the analysis of the genomic structure and function of P. simonii, we have not only revealed the molecular basis for its adaptation to harsh environments but also identified key genes that promote its growth and resistance to pests and diseases. Furthermore, exploring the survival mechanisms of P. simonii has deepened our understanding of its stress resistance traits, including how it effectively copes with abiotic stresses such as drought, salinization, and heavy metal pollution. In genetic breeding, significant progress has been made through the application of modern biotechnology, improving the growth rate and wood quality of P. simonii and enhancing its environmental adaptability and disease resistance. These research findings have not only enriched our knowledge of the biological characteristics of P. simonii but also provided a solid scientific foundation for its application in ecological restoration, forestry production, and environmental management.
基金supported by the Program for New Century Excellent Talents in University(No.NCET-12-0808)National Natural Science Foundation of China(No.31370661)the Fundamental Research Funds for the Central Universities(No.2572014AA26)
文摘The role of plant eIF5A proteins in multiple biological processes, such as protein synthesis regulation, translation elongation, mRNA turnover, programmed cell death and stress tolerance is well known. Toward using these powerful proteins to increase stress tolerance in agricultural plants, in the present study, we cloned and characterized PsneIFSA2 and PsneIFSA4 from young poplar （P. simonii × P. nigra） leaves. The deduced amino acid sequences of PsneIF5A2 and PsneIF5A4 were 98 % similar to each other, and they are orthologs of eIF5A 1 in Arabidopsis. In a subcellular localization analysis, PsneIF5A2 and PsneIF5A4 proteins were localized in the nucleus and cytoplasm, qRT-PCR analysis showed that PsneIF5A2 and PsneIF5A4 were transcribed in poplar flowers, stem, leaves, and roots. In addition, they were also induced by abiotic stresses. Transgenic yeast expressing PsneIF5A2 and PsneIF5A4 had increased salt, heavy metal, osmotic, oxidative tolerance. Our results suggest that PsneIF5A2 and PsneIF5A4 are excellent candidates for genetic engineering to improve salt and heavy metal tolerance in agricultural plants.
基金supported by grants from the Changjiang Scholars Program of the Ministry of Education of China (Prof. Bailian Li)the National Natural Science Foundation of China (Grant Nos. 30600479,30872042)+3 种基金the Major Science Foundation of the Ministry of Education of China (Grant No. 307006)the Foundation for the Author of National Excellent Doctoral Dissertation of the China (Grant No. 200770)the Program for New Century Excellent Talents in University (Grant No. NCET-07-0084)the Introduction of Foreign Advanced Agricultural Science and Technology into China (Grant No. 2009-4-22)
文摘We investigated phenotypic variation ofPopulus simonii from five provenances in northern China. Our results show that significant differences exist between plants of different provenances in morphological and physiological traits, except for leaf-stalk length and the number of leaf margins. The coefficient of variation for all traits ranges from 14.77% to 81.49%. The mean phenotypic coefficient of differentiation （VST） is 47.1%, which means that the variation within provenances is the major source for phenotypic variation in P. simonii. Given our cluster analysis of provenances based on an average linkage computing method, the five provenances ofP. simonii investigated could be divided into three groups. Our results provide a theoretical basis for genetic resource conservation and provenance selection of natural P. simonii in northern China.
基金supported by National Natural Science Foundation of China (30871727 31071817)+1 种基金the Funding Project for Academic Human Resources Development in Institutions of Higher Learning under the Jurisdiction of Beijing Municipality (PXM20090142076309)the Beijing Science and Technology Plan Program (Z080005032508017)
文摘Aldehydes play an important role in atmospheric chemistry and plant direct and indirect defense against environmental stresses.In this study,the emissions of saturated C6-C10 aldehydes from Populus simonii × P.pyramidalis ＇Opera 8277＇ cuttings were examined by using a gas chromatography/mass spectrometry（GC/MS） technique at three levels of light intensity（400,800 and 1 200 μmol·m-2·s-1）.A positive correlation between the emissions of these aldehydes and light intensity was found.Moreover,nordi-hydroguaiaretic acid（NDGA）,a special inhibitor of lipoxygenase（LOX）,significantly inhibited the emissions of C6-C9 aldehydes at three levels of light intensity,but did not influence the emission of decanal（C10）.The emissions of C6-C10 aldehydes in NDGA treated poplar cuttings,exhibited the same positive correlation with light intensity.The results indicated that LOX pathway contributes to the emissions of C6-C9 aldehydes,whereas some pathways regulated by light intensity might be a universal mechanism for emissions of C6-C10 aldehydes.
基金Supported by National Natural Science Foundation of China(31370661)
文摘[ Objective ] This study aimed to analyze the functions of AP1 gene from Populus simonii × Populus nigra and to lay the theoretical foundation for shortening the breeding cycle of forest trees and investigating the flowering mechanism in poplar. [ Method] Plant expression vectors of AP1 genes were constructed and transformed into tobacco leaf disks with Agrobacterium-mediated method. Transgenic tobacco plants were identified by PCR. [ Result] AP1 genes were integrated into the genome of tobacco. Transgenic tobacco plants all presented an early flowering phenotype compared with wild-type tobacco. [ Conclusion] AP1 genes could promote early flowering in transgenic tobacco plants, which provided theoretical basis for molecular regulation of flowering in poplar.
文摘Populus simonii Carr., one of the main poplar tree species, is cultivated widely in Northeast and Northwest China in protection and timber forests. Plant phenology plays an important role in timber production by controlling the growing period (i.e., the period between the leaf unfolding and the leaf turning yellow). It is important to understand this control mechanism and to improve the accuracy of the simulation of leaf unfolding phenology for P. simonii in order to determine accurately the timber production of P. simonii plantations. In this study, based on phenological observation data from 10 agricultural meteorological stations in Heilongjiang Province, China, model simulation was employed to determine the control mechanism of leaf unfolding of P. simonii. Furthermore, the predicting effects of nine phenology-simulating models for P. simonii leaf unfolding were evaluated and the distribution characteristics of P. simonii leaf unfolding in China in 2015 were simulated. The results show that P. simonii leaf unfolding is sensitive to air temperature;consequently, climate warming could advance the P. simonii leaf unfolding process. The phenological model based on air temperature could be better suited for simulating P. simonii leaf unfolding, with 76.7% of the calibration data of absolute error being less than three days. The performance of the models based solely on forcing requirements was found superior to that of the models incorporating chilling. If it was imperative that the chilling threshold is reached, the south of the Yunnan, Guangdong, and Guangxi provinces would be unsuitable for planting P. simonii. In this regard, the phenology model based on the chilling threshold as necessary condition was indicated a more reasonable model for the distribution characteristics of P. simonii leaf unfolding.
