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Siberian silkmoth outbreaks surpassed geoclimatic barrier in Siberian Mountains 被引量:4
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作者 KHARUK Viacheslav I. IM Sergei T. SOLDATOV Vladimir V. 《Journal of Mountain Science》 SCIE CSCD 2020年第8期1891-1900,共10页
Siberian silkmoth(SSM,Dendrolimus sibiricus Tschetv.)is the most important defoliator of Siberian pine(Pinus sibirica Du Tour)and fir(Abies sibirica Ledeb.)stands.Warming-induced SSM outbreaks are one of the major dri... Siberian silkmoth(SSM,Dendrolimus sibiricus Tschetv.)is the most important defoliator of Siberian pine(Pinus sibirica Du Tour)and fir(Abies sibirica Ledeb.)stands.Warming-induced SSM outbreaks are one of the major driving factors of successions within the taiga zone.It is suggested that climate change impacted the SSM range and life cycle.We analyzed the migration of alpine and northerly SSM outbreak boundaries in Siberia and the impact of the climate variables and topography on the outbreak dynamics.We used time-series scenes(multispectral data,and vegetation indexes EVI and NDII)in combination with field studies,climate variables,and GIS techniques.We found that SSM outbreaks in the area of alpine boundary shifted about 370 m uphill since the mid of 1950.The outbreak onset was promoted by increased dryness and active temperatures and decreased root zone moisture content in the spring-early summer period.The terrain topography strongly affected SSM outbreak onset and dynamics.Initially,the outbreak was located at the middle elevations on the gentle concave southeastern slopes,which are the favorable insect habitats between outbreaks.Then the outbreak expanded uphill and downhill,to steeper slopes,and both concave and convex terrains.Alongside with elevation range expansion,SSM surpassed its northern historical outbreak boundary:the potential outbreaks’boundary moved about 300 km northward.Climate warming contributes to SSM migration into former outbreak free conifer stands located in highlands and at northern latitudes. 展开更多
关键词 Insect outbreaks Remote sensing monitoring Insect ranges expansion Siberian silkmoth Forest health Conifer mortality
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柞蚕胚胎对高温胁迫的耐受性及其遗传
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作者 焦阳 姜晓旭 +4 位作者 吴艳 钟亮 戚俐 陈俊山 徐亮 《沈阳农业大学学报》 CAS CSCD 北大核心 2024年第3期316-322,共7页
为探索柞蚕胚胎对高温胁迫的耐受性及其遗传规律,对试验群体辽蚕527及5个亲本10个F1组合的柞蚕受精卵采取43℃以上的高温胁迫。结果表明:高温胁迫对不同发育阶段胚胎的影响存在显著差异。有效积温在5~10℃时对高温胁迫的耐受性最弱,而... 为探索柞蚕胚胎对高温胁迫的耐受性及其遗传规律,对试验群体辽蚕527及5个亲本10个F1组合的柞蚕受精卵采取43℃以上的高温胁迫。结果表明:高温胁迫对不同发育阶段胚胎的影响存在显著差异。有效积温在5~10℃时对高温胁迫的耐受性最弱,而有效积温在20℃时对高温胁迫的耐受性最强。高温胁迫对胚胎生命力的影响存在显著差异,随着温度的升高,孵化率降低。高温胁迫也会延长胚胎的发育时期,并且对孵化整齐度产生重要影响,使孵化时间延长。不同品种对高温胁迫的耐受性存在显著差异,其中辽蚕527对高温胁迫的耐受性最强,小白蚕的耐受性最弱。遗传分析表明:柞蚕胚胎对高温胁迫的耐受性受4对主基因控制,主基因效应明显,其遗传率为91.97%,而微效多基因对其影响较小,遗传率仅为5.77%。综上,通过杂交后代胚胎对高温胁迫耐受性进行早期选择,可以选育出对高温胁迫耐受性强的柞蚕品种。 展开更多
关键词 柞蚕 卵胚胎 高温胁迫 耐受性 遗传
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蚕类昆虫线粒体DNA研究及其在起源与进化研究中的应用 被引量:6
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作者 房守敏 张烈 鲁成 《昆虫知识》 CAS CSCD 北大核心 2010年第3期439-445,共7页
线粒体DNA(mtDNA)属母系遗传,进化速率较核基因快且基因组结构相对简单,已作为理想的分子标记广泛应用于昆虫群体遗传学及分子系统学等研究。本文对蚕类昆虫线粒体DNA在分子水平上的最新研究进展进行了较详细的阐述,重点介绍了蚕类昆虫... 线粒体DNA(mtDNA)属母系遗传,进化速率较核基因快且基因组结构相对简单,已作为理想的分子标记广泛应用于昆虫群体遗传学及分子系统学等研究。本文对蚕类昆虫线粒体DNA在分子水平上的最新研究进展进行了较详细的阐述,重点介绍了蚕类昆虫线粒体基因组的组成及特征、mtDNA克隆与多态性及在蚕类昆虫分子系统学研究中的应用等。 展开更多
关键词 线粒体DNA 蚕类昆虫 多态性 分子系统学
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福建樗蚕生物学特性的观察 被引量:3
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作者 李友恭 陈顺立 赵芳 《福建林学院学报》 CSCD 1991年第4期418-421,共4页
樗蚕是樟树的一种害虫。在福建一年发生二代,以蛹越冬,翌年4月中旬成虫开始羽化,卵产于叶上,幼虫6龄,少数5龄。黑瘤姬蜂、绒茧蜂、广大腿蜂和家蚕追寄蝇是其主要天敌。
关键词 樗蚕 生物学特性 樟树 害虫 福建
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家蚕卵半胱氨酸蛋白酶纯化及抗血清制备 被引量:1
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作者 赵小凡 S.Y.Takahashi 《Zoological Research》 CAS CSCD 1993年第1期93-95,共3页
据报道,家蚕卵中存在半胱氨酸蛋白酶(Cysteine proteinase,CP),其性质与哺乳类溶酶体半胱氨酸蛋白酶类的组织蛋白酶L相似,最佳作用pH为3.5,体外最适作用底物为牛血红蛋白,体内最适作用底物为卵黄磷蛋白。经SDS—PAGE分析,分子量47KD。... 据报道,家蚕卵中存在半胱氨酸蛋白酶(Cysteine proteinase,CP),其性质与哺乳类溶酶体半胱氨酸蛋白酶类的组织蛋白酶L相似,最佳作用pH为3.5,体外最适作用底物为牛血红蛋白,体内最适作用底物为卵黄磷蛋白。经SDS—PAGE分析,分子量47KD。其主要作用是在胚胎发育过程中降解卵黄蛋白质,供胚胎发育之需要。 在成熟卵中具很高含量的半胱氨酸蛋白酶,在胚胎发育开始前,并不发生卵黄蛋白质的水解,其作用机制尚待阐明。为了进一步研究半胱氨酸蛋白酶的组织分布、合成位点、及cDNA克隆等,作者从家蚕卵中纯化了半胱氨酸蛋白酶,并制备了抗血清。 展开更多
关键词 家蚕 半胱氨酸 蛋白酶 提纯
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斑点免疫印迹法快速测定4品种家蚕蛾下颚溶茧酶含量
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作者 王厚伟 《中药材》 CAS CSCD 北大核心 2008年第11期1619-1621,共3页
目的:测定4品种家蚕蛾下颚溶茧酶含量,并建立斑点免疫印迹法在中药材药效成分含量的测定方法。方法:以兔抗溶茧酶多抗为一抗,应用斑点免疫印迹法对4品种家蚕蛾下颚溶茧酶含量作定性检测,杂交信号应用Quantity one软件作定量分析。结果:... 目的:测定4品种家蚕蛾下颚溶茧酶含量,并建立斑点免疫印迹法在中药材药效成分含量的测定方法。方法:以兔抗溶茧酶多抗为一抗,应用斑点免疫印迹法对4品种家蚕蛾下颚溶茧酶含量作定性检测,杂交信号应用Quantity one软件作定量分析。结果:4品种家蚕蛾下颚的个体溶茧酶平均含量分别为6.41、8.09、7.62、1.88μg/头。结论:不同品种家蚕蛾间下颚溶茧酶含量差异显著。斑点免疫印迹法简便快捷,结果准确,无需大型设备,可用于类似中药材药效物质的含量测定与品质评价。 展开更多
关键词 家蚕蛾 溶茧酶 斑点印迹
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Extraction of Natural Silk Fibre from Cocoons of Argema mimosae (Lepidoptera: Saturniidae)
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作者 Boniface Mutua Ngoka Everlyn Kamene Nguku +2 位作者 Esther Ndaisi Kioko Suresh Kumar Raina Jones Mulwa Mueke 《Journal of Agricultural Science and Technology(A)》 2015年第10期846-853,共8页
In recent years, numerous actions for sustainable exploitation of forest resources have been undertaken, but few deal with commercialization of forest insects. Several tree based insect products, like wild silk, can b... In recent years, numerous actions for sustainable exploitation of forest resources have been undertaken, but few deal with commercialization of forest insects. Several tree based insect products, like wild silk, can be linked with forest conservation activities to provide livelihood services of different economic scales to forest dependent people. African wild silkmoth, Argema mimosae Boisduval, is one of the forest insects, a moth in the family of Saturniidae producing wild silk, but there is need for definite procedures for extracting silk fibre from its cocoons. This study evaluated physical characteristics of the A. mimosae cocoons and outlined a procedure to extract viable silk fibre for use in silk industry. Cocoons of the A. mimosae were collected from natural forest of Arabuko-Sokoke, Kenya (3020' S, 39055' E), and others obtained by semi-captive rearing of the silkmoth larvae. The cocoons of A mimosae were described to be silvery in colour, tough and thick with distinctive perforations on the surface and a prominent valve-like opening with loose filaments at one end. The study recorded 120 min as the optimum boiling time for the cocoons to yield silvery brown silk floss using sodium carbonate (Na2CO3) as the degumming agent. Cocoons boiled in distilled water could not be deflossed into floss, but remained hard and intact, as compared with those of the control, Bombyx mori. This reveals that A. mimosae cocoons can be processed for natural silk fibre production and offer communities adjacent to forests an excellent opportunity to engage in production of natural wild silk. 展开更多
关键词 African wild silkmoth A. mimosae COCOONS silk fibre.
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