Objective To determine the diagnostic significance of detecting the specific epithelial keratin CK-20 mRNA in peripheral venous blood from patients with bladder carcinomas. Methods Reverse transcription coupled with t...Objective To determine the diagnostic significance of detecting the specific epithelial keratin CK-20 mRNA in peripheral venous blood from patients with bladder carcinomas. Methods Reverse transcription coupled with two-step polymerase chain reaction (nested RT-PCR) was used to detect CK-20 mRNA expression in the peripheral blood from patients with blodder carcinomas. Results Detection of CK-20 mRNA expression was positive in 37 of 91 patients with bladder carcinoma (41 % ). Among 20 patients with distant metastasis, 17 were positive (85 % ). CK-20 mRNA was not detectable in the blood samples from 25 normal individuals. The frequency of positive CK-20 mRNA expression was signficantly higher in those with distant metastasis. Conclusion The presence of CK-20 mRNA expression in peripheral blood may be used as an early indicator of hematogenous metastasis of bladder carcinoma cells. 6 refs,1 tab.展开更多
In this study,we present a comprehensive analysis of an unidentified pointlike ultra-high-energy(UHE)g-ray source,designated as 1LHAASO J1740+0948u,situated in the vicinity of the middle-aged pulsar PSR J1740+1000.The...In this study,we present a comprehensive analysis of an unidentified pointlike ultra-high-energy(UHE)g-ray source,designated as 1LHAASO J1740+0948u,situated in the vicinity of the middle-aged pulsar PSR J1740+1000.The detection significance reached 17.1 s(9.4 s)above 25 TeV(100 TeV).展开更多
文摘Objective To determine the diagnostic significance of detecting the specific epithelial keratin CK-20 mRNA in peripheral venous blood from patients with bladder carcinomas. Methods Reverse transcription coupled with two-step polymerase chain reaction (nested RT-PCR) was used to detect CK-20 mRNA expression in the peripheral blood from patients with blodder carcinomas. Results Detection of CK-20 mRNA expression was positive in 37 of 91 patients with bladder carcinoma (41 % ). Among 20 patients with distant metastasis, 17 were positive (85 % ). CK-20 mRNA was not detectable in the blood samples from 25 normal individuals. The frequency of positive CK-20 mRNA expression was signficantly higher in those with distant metastasis. Conclusion The presence of CK-20 mRNA expression in peripheral blood may be used as an early indicator of hematogenous metastasis of bladder carcinoma cells. 6 refs,1 tab.
基金supported by the following grants:the National Natural Science Foundation of China nos.12393851,12393852,12393853,12393854,12205314,12105301,12305120,12261160362,12105294,U1931201,12375107,and 12173039the Department of Science and Technology of Sichuan Province,China,no.24NSFSC2319+1 种基金the Project for Young Scientists in Basic Research of Chinese Academy of Sciences no.YSBR-061the NSRF in Thailand via the Program Management Unit for Human Resources and Institutional Development,Research and Innovation(B39G670013).
文摘In this study,we present a comprehensive analysis of an unidentified pointlike ultra-high-energy(UHE)g-ray source,designated as 1LHAASO J1740+0948u,situated in the vicinity of the middle-aged pulsar PSR J1740+1000.The detection significance reached 17.1 s(9.4 s)above 25 TeV(100 TeV).
