[Objectives] To optimize the pet dog dyeing process of plant hair dye. [Methods]Three factors affecting dyeing were selected including pH,concentration of dye and dyeing time. The L_9(3~3) orthogonal design was used t...[Objectives] To optimize the pet dog dyeing process of plant hair dye. [Methods]Three factors affecting dyeing were selected including pH,concentration of dye and dyeing time. The L_9(3~3) orthogonal design was used to evaluate the hair coloring effect and the absorbance of cleaning solution after hair was dyed. [Results] The optimum pet dog dyeing process of plant hair dye was dye concentration of50 mg/mL,dye pH of 7. 5 and dyeing time of 1 h. [Conclusions] The coloring and fixing effects of the dye and dyeing process are good,which can lay a foundation for the development and utilization of plant dyes.展开更多
In this study,polysaccharides from Zanthoxylum schinifolium Siebold and Zucc.leaves(ZLPs)were obtained through five extraction methods:hot water extraction(HWE-CPS),ultrasound-assisted extraction(UAE-CPS),enzymatic ex...In this study,polysaccharides from Zanthoxylum schinifolium Siebold and Zucc.leaves(ZLPs)were obtained through five extraction methods:hot water extraction(HWE-CPS),ultrasound-assisted extraction(UAE-CPS),enzymatic extraction(EAE-CPS),microwave-assisted extraction(MAE-CPS),and microwave-enzyme co-assisted extraction(MA-EAE-CPS).Structural analysis showed that ZLPs consist mainly of galacturonic acid(30.34 mol%-34.24 mol%)and galactose(33.94 mol%-37.59 mol%),with molecular weights(Mw)ranging from 1648 to 3859 kDa.Ultrasound-assisted extraction reduced the Mw.All the ZLPs resisted degradation under simulated gastrointestinal conditions,with UAE-CPS exhibiting the strongest resistance and EAE-CPS the weakest.The prebiotic potentials of HWE-CPS,UAE-CPS,and MA-EAE-CPS were assessed by in vitro colonic fermentation.After 48 h,all the samples were extensively degraded,and the microbial composition(pH declined to 6.13,5.58,and 5.50)led to a significant increase in Bacteroidetes and a decrease in Proteobacteria.Fermentation also elevated short-chain fatty acids(SCFAs)levels,with UAE-CPS yielding the highest total SCFAs concentration(42.42 mmol/L).Correlation analysis indicated that the ratio of the RG-I domain was positively correlated with the abundance of certain beneficial microorganisms and the production of SCFAs.These findings provide valuable insights into polysaccharide extraction from plant leaves and provide a reference for the application of ZLPs as functional prebiotic ingredients.展开更多
基金Supported by the National Science and Technology Foundation Project of Traditional Chinese Veterinary Medicine Resources Rescuing and Organizing(2013FY110600-03)
文摘[Objectives] To optimize the pet dog dyeing process of plant hair dye. [Methods]Three factors affecting dyeing were selected including pH,concentration of dye and dyeing time. The L_9(3~3) orthogonal design was used to evaluate the hair coloring effect and the absorbance of cleaning solution after hair was dyed. [Results] The optimum pet dog dyeing process of plant hair dye was dye concentration of50 mg/mL,dye pH of 7. 5 and dyeing time of 1 h. [Conclusions] The coloring and fixing effects of the dye and dyeing process are good,which can lay a foundation for the development and utilization of plant dyes.
基金supported through the Chongqing Science and Technology Bureau's Experiment and Demonstration Project(CSTB2023TIAD-KPX0031)by a grant from the Chongqing Agri-cultural and Rural Affairs Committee(CQMAITS05).
文摘In this study,polysaccharides from Zanthoxylum schinifolium Siebold and Zucc.leaves(ZLPs)were obtained through five extraction methods:hot water extraction(HWE-CPS),ultrasound-assisted extraction(UAE-CPS),enzymatic extraction(EAE-CPS),microwave-assisted extraction(MAE-CPS),and microwave-enzyme co-assisted extraction(MA-EAE-CPS).Structural analysis showed that ZLPs consist mainly of galacturonic acid(30.34 mol%-34.24 mol%)and galactose(33.94 mol%-37.59 mol%),with molecular weights(Mw)ranging from 1648 to 3859 kDa.Ultrasound-assisted extraction reduced the Mw.All the ZLPs resisted degradation under simulated gastrointestinal conditions,with UAE-CPS exhibiting the strongest resistance and EAE-CPS the weakest.The prebiotic potentials of HWE-CPS,UAE-CPS,and MA-EAE-CPS were assessed by in vitro colonic fermentation.After 48 h,all the samples were extensively degraded,and the microbial composition(pH declined to 6.13,5.58,and 5.50)led to a significant increase in Bacteroidetes and a decrease in Proteobacteria.Fermentation also elevated short-chain fatty acids(SCFAs)levels,with UAE-CPS yielding the highest total SCFAs concentration(42.42 mmol/L).Correlation analysis indicated that the ratio of the RG-I domain was positively correlated with the abundance of certain beneficial microorganisms and the production of SCFAs.These findings provide valuable insights into polysaccharide extraction from plant leaves and provide a reference for the application of ZLPs as functional prebiotic ingredients.
