Phosphophoryn (PP) and dentin sialoprotein (DSP) are the most dominant non-collagenous proteins in dentin. PP is an extremely acidic protein that can function as a mineral nucleator for dentin mineralization. DSP was ...Phosphophoryn (PP) and dentin sialoprotein (DSP) are the most dominant non-collagenous proteins in dentin. PP is an extremely acidic protein that can function as a mineral nucleator for dentin mineralization. DSP was first identified in 1981, yet its functional significance is still controversial. Historically, these two proteins were considered to be independently synthesized and secreted by dental pulp cells into the developing dentin matrix. However, with the identification of the DSP coding sequence in 1994, followed2 years later by the finding that the PP coding sequence was located immediately downstream from the DSP sequence, it became immediately clear that DSP and PP proteins were derived from a single DSP-PP (i.e., dentin sialophosphoprotein, DSPP) transcript.Since DSPP cDNA became available, tremendous progress has been made in studying DSP-PP mRNA distribution and DSP generation from the DSP-PP precursor protein at specific cleavage sites by protease tolloid-related-1 (TLR1) or bone morphogenetic protein 1 (BMP1). The functions of DSP-PP and DSP were investigated via DSP-PP knockout (KO) and DSP knockin in DSP-PP KO mice. In addition, a number of in vitro studies aimed to elucidate DSPP and DSP function in dental pulp cells.展开更多
Summary： This study aimed to evaluate the diagnostic and prognostic significance of serum bone sialoprotein （BSP） and prostate-specific antigen doubling time （PSADT） in patients with bone metastasis （BM） from p...Summary： This study aimed to evaluate the diagnostic and prognostic significance of serum bone sialoprotein （BSP） and prostate-specific antigen doubling time （PSADT） in patients with bone metastasis （BM） from prostate cancer （PC）. A total of 116 patients with PC, 120 patients with benign prostatic hyperplasia （BPH） and 120 healthy controls were enrolled in this study. PC patients were divided into bone metastasis （BM） group （n=56） and non-bone metastasis （NBM） group （n=60）. Serum BSP was detected by Sandwich ELISA. Severity of bone pain was evaluated using visual analogue score （VAS）. Serum f-PSA and t-PSA levels were measured by using electrochemiluminescence immunoassay （ECLIA）. PSADT was calculated according to the formula： PSADT=lg（2）/[log（PSA2）--log（PSA1）]. The mean serum BSP level in PC patients with BM was significantly higher than in PC patients without BM, BPH patients and controls （P〈0.001 for all）. Pearson＇s analysis showed that serum BSP level was posi- tively correlated with VAS in PC patients with BM （P〈0.05）. Receiver operating characteristics （ROC） analysis demonstrated that BSP discriminated patients with BM from those without BM at the cutoff value of 33.26 ng/mL. The sensitivity and specificity were 78.21% and 79.28%, respectively. The opti- mal cutoff value of PSADT was 131 days, with sensitivity of 85.69% and specificity of 85.36%. Kap- lan-Meier analysis revealed that subjects with higher BSP levels/shorter PSADT had a shorter BM-free period than those with lower BSP levels/longer PSADT. Serum BSP and PSADT are useful biomarkers for the diagnosis of BM from PC, and can be regarded as independent factors for predicting the progno- sis of BM from PC. Combined determination of BSP and PSADT can improve accuracy and positive rate of BM from PC significantly.展开更多
Bone sialoprotein(BSP)is an important non-collagen extracellular matrix protein(EMC)that promotes bone formation and induces bone resorption.BSP is secreted by odontoblasts,it plays an important role in cementum,alveo...Bone sialoprotein(BSP)is an important non-collagen extracellular matrix protein(EMC)that promotes bone formation and induces bone resorption.BSP is secreted by odontoblasts,it plays an important role in cementum,alveolar bone formation and mineralization,and periodontal function.Bone resorption is controlled by a complex molecular network,and BSP can promote osteoclast differentiation and bone resorption.It is also associated with the metastasis of a range of malignancies.Osteoclasts(OC)are thought to be the only cells involved in bone resorption and play an important role in bone formation and late developmental remodeling.Osteoporosis and periodontal disease are caused by excessive bone resorption.This article will summarize the osteoclasts differentiation,the biological function of bone resorption,and explore the progress of the prevention and treatment of the related bone resorption diseases such as osteoporosis and periodontal disease through the regulation of osteoclasts.展开更多
Objective With the unique properties of Au nano-particles,a nano-piezoelectric immunosensor microarray was designed using AT-cut 10 Hz quartz crystal to detect blood sialoprotein (BSP) expression from clinical serum.M...Objective With the unique properties of Au nano-particles,a nano-piezoelectric immunosensor microarray was designed using AT-cut 10 Hz quartz crystal to detect blood sialoprotein (BSP) expression from clinical serum.Methods展开更多
Objective To construct transgenic vectors of mouse DSP.Methods To construct pcDNA3.1 CX by substituting CMV promoter of pcDNA3.1 with promoter cβ actin,and establish the ultimate transgenic vector by cloning DSP codi...Objective To construct transgenic vectors of mouse DSP.Methods To construct pcDNA3.1 CX by substituting CMV promoter of pcDNA3.1 with promoter cβ actin,and establish the ultimate transgenic vector by cloning DSP coding sequence into pcDNA3.1 CX.Result s Enzyme digestion and sequencing are consistent with expected.Conclusion The transgenic vector of mouse DSP was constructed successfully.展开更多
OBJECTIVE:To prepare PLGA microsphere of BSP.METHODS:mulsion solvent extraction-evaporation process was used to prepare microsphere.Technology of preparation was optimized by the orthogonal design.The surface morpholo...OBJECTIVE:To prepare PLGA microsphere of BSP.METHODS:mulsion solvent extraction-evaporation process was used to prepare microsphere.Technology of preparation was optimized by the orthogonal design.The surface morphology of the microsphere was observed by EM and SEM.The drug loading,the encapsulation efficiency and release rate in vitro were measured using micro-BCA kit.RESULTS:The mean particle size of the microsphere was 50±0.5μm,and its mean span was 0.58±0.10,the drug loading and the encapsulation efficiency were(0.725±0.075)%and(64.876±0.680)%respectively.The reappearance of pharmaceutical technology was good.CONCLUSION:this research described the optimized preparation technology of PLGA microspheres of BSP.展开更多
背景:Siblings家族蛋白为一种外分泌蛋白家族,可由多种细胞分泌至细胞外基质,使其黏附于细胞外基质胶原,反过来作用于细胞,产生相应的生理、病理作用。目的:归纳现阶段关于Siblings家族蛋白在心血管疾病方面的研究。方法:通过计算机对Pu...背景:Siblings家族蛋白为一种外分泌蛋白家族,可由多种细胞分泌至细胞外基质,使其黏附于细胞外基质胶原,反过来作用于细胞,产生相应的生理、病理作用。目的:归纳现阶段关于Siblings家族蛋白在心血管疾病方面的研究。方法:通过计算机对PubMed、Web of Science、Engineering Village及中国知网数据库相关文献进行检索,检索时限为2008年1月至2024年3月,英文检索词为“Current research status,Siblings,Siblings protein family,Cardiovascular,Cardiovascular diseases”;中文检索词为“研究进展、Siblings家族蛋白、心血管、心血管疾病”,依据入选标准对检索结果进行筛选排除,最终纳入76篇文献进行综述分析。结果与结论:(1)Siblings家族蛋白在心血管多种疾病中起了很大的作用,尤其是在瓣膜钙化、血管硬化的疾病中有十分大的研究潜力。(2)部分Siblings家族蛋白可以沉积钙盐形成羟基磷灰石核心,开始形成微钙化结节,并通过RGD肽段与细胞表面的整合素受体结合促进细胞成骨样分化的正反馈机制,增加自身的表达量。(3)整合素受体可成为治疗心血管钙化性疾病的潜在靶点,临床可通过阻断整合素受体达到延缓钙化进程的目的。展开更多
目的:探讨骨代谢生化指标血清Ⅰ型胶原交联氨基末端肽(N-telopeptide of typeⅠcollagen,NTx)和骨涎酸蛋白(bone sialoprotein,BSP)的检测对肺癌和乳腺癌骨转移的临床意义。方法:选择2006年1月至2006年7月长海医院肿瘤科经病理确诊的肺...目的:探讨骨代谢生化指标血清Ⅰ型胶原交联氨基末端肽(N-telopeptide of typeⅠcollagen,NTx)和骨涎酸蛋白(bone sialoprotein,BSP)的检测对肺癌和乳腺癌骨转移的临床意义。方法:选择2006年1月至2006年7月长海医院肿瘤科经病理确诊的肺癌或乳腺癌患者105例,分为两组,其中骨转移组50例,无骨转移组55例。应用ELISA法检测患者血清NTx和BSP浓度。结果:骨转移组患者血清NTx和BSP水平均明显高于无骨转移组(P<0.01)。NTx诊断骨转移的灵敏度和特异度分别为90.0%和67.3%,BSP诊断骨转移的灵敏度和特异度分别为84.0%和70.9%。临床发生骨相关事件(skeletal related event,SRE)的骨转移患者,血清NTx水平明显高于未发生SRE的骨转移患者(P<0.05)。在6～13个月随访期内,21例患者确诊了新发骨转移;采用Cox比例风险回归模型分析,血清NTx浓度升高提示骨转移发生的相对危险度为1.127;乳腺癌患者血清BSP增高是唯一预测骨转移的危险因素(P<0.05),其相对危险度为1.058。随访期共有33例患者死亡;无论肺癌还是乳腺癌,血清BSP增高患者的累积生存率均明显低于血清BSP正常组(P<0.05)。结论:血清NTx和BSP是诊断肺癌和乳腺癌骨转移的重要参考指标,其水平的增高是预测骨转移发生的高危因素;BSP可能是肺癌和乳腺癌患者独立预后指标。展开更多
文摘Phosphophoryn (PP) and dentin sialoprotein (DSP) are the most dominant non-collagenous proteins in dentin. PP is an extremely acidic protein that can function as a mineral nucleator for dentin mineralization. DSP was first identified in 1981, yet its functional significance is still controversial. Historically, these two proteins were considered to be independently synthesized and secreted by dental pulp cells into the developing dentin matrix. However, with the identification of the DSP coding sequence in 1994, followed2 years later by the finding that the PP coding sequence was located immediately downstream from the DSP sequence, it became immediately clear that DSP and PP proteins were derived from a single DSP-PP (i.e., dentin sialophosphoprotein, DSPP) transcript.Since DSPP cDNA became available, tremendous progress has been made in studying DSP-PP mRNA distribution and DSP generation from the DSP-PP precursor protein at specific cleavage sites by protease tolloid-related-1 (TLR1) or bone morphogenetic protein 1 (BMP1). The functions of DSP-PP and DSP were investigated via DSP-PP knockout (KO) and DSP knockin in DSP-PP KO mice. In addition, a number of in vitro studies aimed to elucidate DSPP and DSP function in dental pulp cells.
基金supported by a grant from the Scientific and Technological Department of Hunan Province in 2005(No.05FJ3033)
文摘Summary： This study aimed to evaluate the diagnostic and prognostic significance of serum bone sialoprotein （BSP） and prostate-specific antigen doubling time （PSADT） in patients with bone metastasis （BM） from prostate cancer （PC）. A total of 116 patients with PC, 120 patients with benign prostatic hyperplasia （BPH） and 120 healthy controls were enrolled in this study. PC patients were divided into bone metastasis （BM） group （n=56） and non-bone metastasis （NBM） group （n=60）. Serum BSP was detected by Sandwich ELISA. Severity of bone pain was evaluated using visual analogue score （VAS）. Serum f-PSA and t-PSA levels were measured by using electrochemiluminescence immunoassay （ECLIA）. PSADT was calculated according to the formula： PSADT=lg（2）/[log（PSA2）--log（PSA1）]. The mean serum BSP level in PC patients with BM was significantly higher than in PC patients without BM, BPH patients and controls （P〈0.001 for all）. Pearson＇s analysis showed that serum BSP level was posi- tively correlated with VAS in PC patients with BM （P〈0.05）. Receiver operating characteristics （ROC） analysis demonstrated that BSP discriminated patients with BM from those without BM at the cutoff value of 33.26 ng/mL. The sensitivity and specificity were 78.21% and 79.28%, respectively. The opti- mal cutoff value of PSADT was 131 days, with sensitivity of 85.69% and specificity of 85.36%. Kap- lan-Meier analysis revealed that subjects with higher BSP levels/shorter PSADT had a shorter BM-free period than those with lower BSP levels/longer PSADT. Serum BSP and PSADT are useful biomarkers for the diagnosis of BM from PC, and can be regarded as independent factors for predicting the progno- sis of BM from PC. Combined determination of BSP and PSADT can improve accuracy and positive rate of BM from PC significantly.
基金National Natural Science Foundation Project(No.81260275)。
文摘Bone sialoprotein(BSP)is an important non-collagen extracellular matrix protein(EMC)that promotes bone formation and induces bone resorption.BSP is secreted by odontoblasts,it plays an important role in cementum,alveolar bone formation and mineralization,and periodontal function.Bone resorption is controlled by a complex molecular network,and BSP can promote osteoclast differentiation and bone resorption.It is also associated with the metastasis of a range of malignancies.Osteoclasts(OC)are thought to be the only cells involved in bone resorption and play an important role in bone formation and late developmental remodeling.Osteoporosis and periodontal disease are caused by excessive bone resorption.This article will summarize the osteoclasts differentiation,the biological function of bone resorption,and explore the progress of the prevention and treatment of the related bone resorption diseases such as osteoporosis and periodontal disease through the regulation of osteoclasts.
文摘Objective With the unique properties of Au nano-particles,a nano-piezoelectric immunosensor microarray was designed using AT-cut 10 Hz quartz crystal to detect blood sialoprotein (BSP) expression from clinical serum.Methods
文摘Objective To construct transgenic vectors of mouse DSP.Methods To construct pcDNA3.1 CX by substituting CMV promoter of pcDNA3.1 with promoter cβ actin,and establish the ultimate transgenic vector by cloning DSP coding sequence into pcDNA3.1 CX.Result s Enzyme digestion and sequencing are consistent with expected.Conclusion The transgenic vector of mouse DSP was constructed successfully.
基金supported by Key Projects in Guangdong Province Natural Science Foundation(No.06104396)Research Team Project in Guangdong Province Natural Science Foundation(No.20023001).
文摘OBJECTIVE:To prepare PLGA microsphere of BSP.METHODS:mulsion solvent extraction-evaporation process was used to prepare microsphere.Technology of preparation was optimized by the orthogonal design.The surface morphology of the microsphere was observed by EM and SEM.The drug loading,the encapsulation efficiency and release rate in vitro were measured using micro-BCA kit.RESULTS:The mean particle size of the microsphere was 50±0.5μm,and its mean span was 0.58±0.10,the drug loading and the encapsulation efficiency were(0.725±0.075)%and(64.876±0.680)%respectively.The reappearance of pharmaceutical technology was good.CONCLUSION:this research described the optimized preparation technology of PLGA microspheres of BSP.
文摘背景:Siblings家族蛋白为一种外分泌蛋白家族,可由多种细胞分泌至细胞外基质,使其黏附于细胞外基质胶原,反过来作用于细胞,产生相应的生理、病理作用。目的:归纳现阶段关于Siblings家族蛋白在心血管疾病方面的研究。方法:通过计算机对PubMed、Web of Science、Engineering Village及中国知网数据库相关文献进行检索,检索时限为2008年1月至2024年3月,英文检索词为“Current research status,Siblings,Siblings protein family,Cardiovascular,Cardiovascular diseases”;中文检索词为“研究进展、Siblings家族蛋白、心血管、心血管疾病”,依据入选标准对检索结果进行筛选排除,最终纳入76篇文献进行综述分析。结果与结论:(1)Siblings家族蛋白在心血管多种疾病中起了很大的作用,尤其是在瓣膜钙化、血管硬化的疾病中有十分大的研究潜力。(2)部分Siblings家族蛋白可以沉积钙盐形成羟基磷灰石核心,开始形成微钙化结节,并通过RGD肽段与细胞表面的整合素受体结合促进细胞成骨样分化的正反馈机制,增加自身的表达量。(3)整合素受体可成为治疗心血管钙化性疾病的潜在靶点,临床可通过阻断整合素受体达到延缓钙化进程的目的。
文摘目的:探讨骨代谢生化指标血清Ⅰ型胶原交联氨基末端肽(N-telopeptide of typeⅠcollagen,NTx)和骨涎酸蛋白(bone sialoprotein,BSP)的检测对肺癌和乳腺癌骨转移的临床意义。方法:选择2006年1月至2006年7月长海医院肿瘤科经病理确诊的肺癌或乳腺癌患者105例,分为两组,其中骨转移组50例,无骨转移组55例。应用ELISA法检测患者血清NTx和BSP浓度。结果:骨转移组患者血清NTx和BSP水平均明显高于无骨转移组(P<0.01)。NTx诊断骨转移的灵敏度和特异度分别为90.0%和67.3%,BSP诊断骨转移的灵敏度和特异度分别为84.0%和70.9%。临床发生骨相关事件(skeletal related event,SRE)的骨转移患者,血清NTx水平明显高于未发生SRE的骨转移患者(P<0.05)。在6～13个月随访期内,21例患者确诊了新发骨转移;采用Cox比例风险回归模型分析,血清NTx浓度升高提示骨转移发生的相对危险度为1.127;乳腺癌患者血清BSP增高是唯一预测骨转移的危险因素(P<0.05),其相对危险度为1.058。随访期共有33例患者死亡;无论肺癌还是乳腺癌,血清BSP增高患者的累积生存率均明显低于血清BSP正常组(P<0.05)。结论:血清NTx和BSP是诊断肺癌和乳腺癌骨转移的重要参考指标,其水平的增高是预测骨转移发生的高危因素;BSP可能是肺癌和乳腺癌患者独立预后指标。
