Objective:To evaluate the efficacy and safety of sheathless and fluoroscopy-free flexible ureterorenoscopic laser lithotripsy(FURSL)for treating renal stones.Methods:Between May 2015 and May 2017,135 patients with ren...Objective:To evaluate the efficacy and safety of sheathless and fluoroscopy-free flexible ureterorenoscopic laser lithotripsy(FURSL)for treating renal stones.Methods:Between May 2015 and May 2017,135 patients with renal stones treated with sheathless and fluoroscopy-free FURSL were prospectively evaluated.Our technique involved a semirigid ureteroscopic assessment of the ureter,and the guidewire was left in situ to railroad the flexible ureteroscope.A holmium laser was used to fragment and dust the stones;fragments were neither grasped nor collected.Results:The study population consisted of 135 patients including 85 males(62.96%)and 50 females(37.04%)with a mean age of 40.65 years(range:3-70 years)were evaluated.The mean stone size was 17.23 mm(range:8-41 mm).Complete stone-free status was achieved in 122(90.37%)patients and clinically insignificant residual fragments(CIRF)in two(1.48%),while residual stones were still present in 11(8.15%)patients.Postoperative complications occurred in 23(17.4%)cases and were mostly minor,including fever in 17(12.6%),pyelonephritis in four(3.0%),subcapsular hematoma in one(0.7%)and steinstrasse in one(0.7%).These complications were Clavien Ⅰ-Ⅱ,GⅠ in 17(12.6%)patients,GⅡ in five(3.7%),and Clavien Ⅲb in one(0.7%).No major complications were observed.Stone size2 cm,operative time ≥30 min,and lasing time ≥20 min were significantly associated with a higher rate of complications and lower stone-free rates upon univariate analysis(p<0.05).Conclusion:Sheathless and fluoroscopy-free FURSL are effective and safe for renal stone management,especially for stones under 2 cm in diameter.This process is a feasible option for avoiding sheath complications,which can protect surgeons from the negative effects of radiation.展开更多
Haematococcus pluvialis(H.pluvialis)is a critical natural source of astaxanthin,recognized for its potent antioxidantcapacity,anti-inflammatory properties,and ability to suppress the proliferation of breast and skin c...Haematococcus pluvialis(H.pluvialis)is a critical natural source of astaxanthin,recognized for its potent antioxidantcapacity,anti-inflammatory properties,and ability to suppress the proliferation of breast and skin cancer cells.The sizedifference between microalgae such as Chlorella vulgaris(C.vulgaris)and Haematococcus pluvialis(H.pluvialis),coupledwith their high research value,makes size-based separation of microalgae essential for efficient extraction of valuablespecies and advancing directed algal evolution.In this study,we introduce an innovative method utilizing microfluidicdevices that integrate spiral channels with contraction-expansion channels.We systematically examine how variablessuch as flow rate,cell concentration,cell size,and three distinct coupling configurations impact cell sorting.Our resultshighlight that each coupling configuration of the spiral and contraction-expansion channels exerts unique controlover sorting performance,offering promising new approaches for optimizing microchannel design.展开更多
文摘Objective:To evaluate the efficacy and safety of sheathless and fluoroscopy-free flexible ureterorenoscopic laser lithotripsy(FURSL)for treating renal stones.Methods:Between May 2015 and May 2017,135 patients with renal stones treated with sheathless and fluoroscopy-free FURSL were prospectively evaluated.Our technique involved a semirigid ureteroscopic assessment of the ureter,and the guidewire was left in situ to railroad the flexible ureteroscope.A holmium laser was used to fragment and dust the stones;fragments were neither grasped nor collected.Results:The study population consisted of 135 patients including 85 males(62.96%)and 50 females(37.04%)with a mean age of 40.65 years(range:3-70 years)were evaluated.The mean stone size was 17.23 mm(range:8-41 mm).Complete stone-free status was achieved in 122(90.37%)patients and clinically insignificant residual fragments(CIRF)in two(1.48%),while residual stones were still present in 11(8.15%)patients.Postoperative complications occurred in 23(17.4%)cases and were mostly minor,including fever in 17(12.6%),pyelonephritis in four(3.0%),subcapsular hematoma in one(0.7%)and steinstrasse in one(0.7%).These complications were Clavien Ⅰ-Ⅱ,GⅠ in 17(12.6%)patients,GⅡ in five(3.7%),and Clavien Ⅲb in one(0.7%).No major complications were observed.Stone size2 cm,operative time ≥30 min,and lasing time ≥20 min were significantly associated with a higher rate of complications and lower stone-free rates upon univariate analysis(p<0.05).Conclusion:Sheathless and fluoroscopy-free FURSL are effective and safe for renal stone management,especially for stones under 2 cm in diameter.This process is a feasible option for avoiding sheath complications,which can protect surgeons from the negative effects of radiation.
基金supported by National Natural Science Foundation of China(grant numbers 52075138,61964006 and 62364011)Hainan Province Science and Technology Special Fund(grant numbers ZDYF2022SHFZ301).
文摘Haematococcus pluvialis(H.pluvialis)is a critical natural source of astaxanthin,recognized for its potent antioxidantcapacity,anti-inflammatory properties,and ability to suppress the proliferation of breast and skin cancer cells.The sizedifference between microalgae such as Chlorella vulgaris(C.vulgaris)and Haematococcus pluvialis(H.pluvialis),coupledwith their high research value,makes size-based separation of microalgae essential for efficient extraction of valuablespecies and advancing directed algal evolution.In this study,we introduce an innovative method utilizing microfluidicdevices that integrate spiral channels with contraction-expansion channels.We systematically examine how variablessuch as flow rate,cell concentration,cell size,and three distinct coupling configurations impact cell sorting.Our resultshighlight that each coupling configuration of the spiral and contraction-expansion channels exerts unique controlover sorting performance,offering promising new approaches for optimizing microchannel design.
