Guided by TNF-α secretion inhibitory activity assay, four taraxastane-type triterpenoids, including two new ones, 22-oxo-20-taraxasten-3β, 30-diol(1) and 22α-hydroxy-20-taraxasten-30β, 30-triol(2), have been obtai...Guided by TNF-α secretion inhibitory activity assay, four taraxastane-type triterpenoids, including two new ones, 22-oxo-20-taraxasten-3β, 30-diol(1) and 22α-hydroxy-20-taraxasten-30β, 30-triol(2), have been obtained from an active fraction of the petroleum ether-soluble extract of the the medicinal and edible plant Cirsium setosum. Their structures were elucidated by spectroscopic data and CD data analysis. In the TNF-α secretion inhibitory activity assay, compounds 1 and 2 were active with the IC50 of 2.6 and 3.8 μmol·L-1, respectively. In addition, compounds 1 and 2 showed moderately selective cytotoxicity against the human ovarian cancer(A2780) and colon cancer(HCT-8) cell lines.展开更多
试验以吸附率与解析率为考察指标,比较4种大孔吸附树脂D101、D4020、AB-8、X-5对小蓟(Cirsium setosum)多糖的纯化效果。采用分光光度法测定小蓟多糖的含量,用静态与动态吸附-解析方法从4种树脂中筛选出最适树脂,采用单因素试验和正交...试验以吸附率与解析率为考察指标,比较4种大孔吸附树脂D101、D4020、AB-8、X-5对小蓟(Cirsium setosum)多糖的纯化效果。采用分光光度法测定小蓟多糖的含量,用静态与动态吸附-解析方法从4种树脂中筛选出最适树脂,采用单因素试验和正交试验优化其纯化工艺。结果表明,AB-8型大孔树脂为纯化小蓟多糖的最佳树脂;其纯化最佳工艺条件为:上样液浓度2.0 mg/m L,上样量30 m L,流速1.0 m L/min,洗脱剂p H 7.0。在此工艺条件下,AB-8对小蓟多糖的解析率可达77.93%。展开更多
目的:对小蓟的化学成分进行研究。方法:运用硅胶柱层析、Sephadex LH-20柱层析等手段进行分离,运用UV、1HNMR、13CNMR、MS等波谱方法进行结构鉴定。结果:共分离鉴定了6个化合物,分别为乙酸橙酰胺(auran-tiam ide acetate,1),loliolide(...目的:对小蓟的化学成分进行研究。方法:运用硅胶柱层析、Sephadex LH-20柱层析等手段进行分离,运用UV、1HNMR、13CNMR、MS等波谱方法进行结构鉴定。结果:共分离鉴定了6个化合物,分别为乙酸橙酰胺(auran-tiam ide acetate,1),loliolide(2),刺槐素(acacetin,3),蒙花苷(linarin,4),洋芹素-7-O-β-D-葡萄糖醛酸丁酯(ap igen in-7-O-β-D-butylglucuron ide,5),β-胡萝卜苷(β-daucosterol,6)。结论:化合物1为首次从蓟属植物中分离得到,化合物2、5、6首次从小蓟中分离得到。展开更多
基金supported by the Key projects of the Beijing Natural Sciences Foundation and Beijing Municipal Education Committee(No.KZ201811417049)the National Natural Science Foundation of China(Nos.81522050,81773589 and 81760783)+1 种基金the Science and Technology program of Qinghai(No.2018-ZJ-739)the Scientific Research Common Program of Beijing Municipal Commission of Education(No.KM201811417008)
文摘Guided by TNF-α secretion inhibitory activity assay, four taraxastane-type triterpenoids, including two new ones, 22-oxo-20-taraxasten-3β, 30-diol(1) and 22α-hydroxy-20-taraxasten-30β, 30-triol(2), have been obtained from an active fraction of the petroleum ether-soluble extract of the the medicinal and edible plant Cirsium setosum. Their structures were elucidated by spectroscopic data and CD data analysis. In the TNF-α secretion inhibitory activity assay, compounds 1 and 2 were active with the IC50 of 2.6 and 3.8 μmol·L-1, respectively. In addition, compounds 1 and 2 showed moderately selective cytotoxicity against the human ovarian cancer(A2780) and colon cancer(HCT-8) cell lines.
文摘试验以吸附率与解析率为考察指标,比较4种大孔吸附树脂D101、D4020、AB-8、X-5对小蓟(Cirsium setosum)多糖的纯化效果。采用分光光度法测定小蓟多糖的含量,用静态与动态吸附-解析方法从4种树脂中筛选出最适树脂,采用单因素试验和正交试验优化其纯化工艺。结果表明,AB-8型大孔树脂为纯化小蓟多糖的最佳树脂;其纯化最佳工艺条件为:上样液浓度2.0 mg/m L,上样量30 m L,流速1.0 m L/min,洗脱剂p H 7.0。在此工艺条件下,AB-8对小蓟多糖的解析率可达77.93%。
文摘目的:对小蓟的化学成分进行研究。方法:运用硅胶柱层析、Sephadex LH-20柱层析等手段进行分离,运用UV、1HNMR、13CNMR、MS等波谱方法进行结构鉴定。结果:共分离鉴定了6个化合物,分别为乙酸橙酰胺(auran-tiam ide acetate,1),loliolide(2),刺槐素(acacetin,3),蒙花苷(linarin,4),洋芹素-7-O-β-D-葡萄糖醛酸丁酯(ap igen in-7-O-β-D-butylglucuron ide,5),β-胡萝卜苷(β-daucosterol,6)。结论:化合物1为首次从蓟属植物中分离得到,化合物2、5、6首次从小蓟中分离得到。
