Nocardia seriolae alters macrophages to a“foamy”phenotype characterized by the accumulation of lipid droplets during infection to create a habitat that is ideally suited to its requirements.However,little is known a...Nocardia seriolae alters macrophages to a“foamy”phenotype characterized by the accumulation of lipid droplets during infection to create a habitat that is ideally suited to its requirements.However,little is known about how exactly it does this.We extracted and identified specific lipids from N.seriolae,mycolic acids(NsMA),and confirmed they induced foam cells for granuloma formation in a snakehead(Channa argus)model.RNA-sequencing revealed that NsMA-induced foam cells were associated with Coronin-1A,which was further supported by Coronin-1A binding to NsMA as well as to the wild-type strain ZJ0503 rather than the NsMA-deficient strainΔfadD32.An in vitro assay revealed that the NsMA-induced Coronin-1A was responsible for inhibiting autophagy,whereas the inhibitory effect of autophagy was reversed in macrophages before receiving an anti-Coronin-1A antibody or mTOR inducer treatment.Meanwhile,the same results were also found by RNA interfering in vivo:silencing coronin-1A activated autophagy in NsMA-challenged snakehead,thereby alleviating the symptoms of granuloma formation with foam cell populations.Taken together,these results demonstrate that NsMA depends on the host's Coronin-1A to inhibit autophagy through the mTOR pathway,and thereby promotes cell foam for granuloma formation in N.seriolae-infected fish.展开更多
Sex-biased microRNAs(miRNAs)influence gonadal development in fish by directly targeting genes associated with estrogen production pathways.WW domain-binding protein 2(WBP2)functions as a crucial transcriptional co-act...Sex-biased microRNAs(miRNAs)influence gonadal development in fish by directly targeting genes associated with estrogen production pathways.WW domain-binding protein 2(WBP2)functions as a crucial transcriptional co-activator of the estrogen and progesterone receptors(PGR).This study investigates the direct modulation of a sex-biased miR-133b on wbp2 and its regulatory role in gonadal development in fish,the greater amberjack(Seriola dumerili).Using dual-luciferase reporter assays,we demonstrate that wbp2 is a direct target of miR-133b,with miR-133b-3p binding to the 3'untranslated region(3'UTR)of wbp2.In vitro,miR-133b mimic significantly downregulate wbp2 expression,while the miR-133b inhibitor increase wbp2 levels.Consistently,in vivo,wbp2 expression is upregulated following antagomir-133b treatment and downregulated following agomir-133b.RNA fluorescence in situ hybridization(RNA-FISH)results reveal miR-133b and wbp2 co-localization in ovarian interstitial cells.Notably,phylogenetic analysis indicates that miR-133b-3p and wbp2 are highly conserved among bony fish species.Additionally,dual-luciferase assays in other bony fish species including Oreochromis niloticus and Danio rerio also confirm the targeting effect of miR-133b-3p on wbp2,suggesting that this regulatory mechanism is conserved across bony fish.This research provides a theoretical foundation for further exploration of non-coding RNA-mediated regulation in gonadal development in teleost.展开更多
Bacterin was prepared by formalin-inactivating the virulent strain of Vibrio hollisae isolated from diseased Seriola dumerili （amberjack） suffering from vibriosis. Healthy S. dumeriIi were vaccinated by respective p...Bacterin was prepared by formalin-inactivating the virulent strain of Vibrio hollisae isolated from diseased Seriola dumerili （amberjack） suffering from vibriosis. Healthy S. dumeriIi were vaccinated by respective procedures of intramuscular injection, immersion, and orally administration. Results of the three different vaccinations were compared. Blood was drawn from the vaccinated fish every 7 days, and the antibody titers and lysozyme activities of the sera were determined. The antibody titer of injected fish was 1：40 at 7 d, and reached its peak of 1：320 at 28 d, while the fish vaccinated by immersion and orally administration exhibited weak antibody responses, the antibody titres of 〈1：10, 1：20, 1：160 were observed at 7 d, 14 d, 35 d respectively. Compared with the control, the vaccinated fish exhibited significantly higher lysozyme activities （P〈0.05）. Upon challenge with virulent strain, the relative percent survival （RPS） of injected, immersed and oral administrated fish were 75%, 45%, and 40% respectively, and the injected fish showed significantly higher RPS than immersed and oral administrated fish. The results suggested that vaccination of S. dumerili by the injection would be the best strategy to prevent the vibriosis in S. dumerili farm.展开更多
Myogenic Regulatory Factors(MRFs)is involved in the muscle growth and differentiation.In this study,the cDNA sequence of yellowtail kingfish MRFs genes were cloned by rapid amplification of cDNA ends(RACE)method;then,...Myogenic Regulatory Factors(MRFs)is involved in the muscle growth and differentiation.In this study,the cDNA sequence of yellowtail kingfish MRFs genes were cloned by rapid amplification of cDNA ends(RACE)method;then,the character-istics of these genes and the predicted protein sequences were analyzed by bioinformatics methods,the tissue and embryonic stages differential expression pattern were detected by the quantitative real-time PCR.Our results showed that the yellowtail kingfish(YTK)Myf5 cDNA has a full length of 951 bp,encoding 266 amino acids.The yellowtail kingfish Myf6 cDNA has a full length of 1105bp,encoding 250 amino acids.The proteins containα-helix,β-strand,and loops.The Neighbour-joining tree revealed that YTK Myf5 and Myf6 are closely related to Seriola dumerili.The yellowtail kingfish Myf5 and Myf6 gene expressed significantly higher in mus-cle than in other tissues(P<0.05).In addition,Myf5 and Myf6 in muscle was significantly expressed in 400g and 500 g fish but not in 50 g,suggesting that myogenic regulatory factors expression had a great relationship with the fish size.Our results also indicated that Myf5 and Myf6 have different functions during embryonic development,because Myf5 showed highest expression level at the neuroembryo period,but Myf6 had the highest expression level at embryo coverage yolk 70%stage.Myf5 gene showed highest ex-pression at 30 d of age,suggesting it played key roles in myogenic period.However,the Myf6 gene was significantly highly ex-pressed at 60 d,revealing this gene functioned in the later muscle formation period.展开更多
Nano-additives can improve the absorption and utilization of feed nutrients.In this study,we reported the effects of supplementary nano iron(0,30,60,120,240 and 480 mg/kg)on intestinal tract function of largemouth bas...Nano-additives can improve the absorption and utilization of feed nutrients.In this study,we reported the effects of supplementary nano iron(0,30,60,120,240 and 480 mg/kg)on intestinal tract function of largemouth bass juveniles.Firstly,60 mg/kg of supplementary nano iron improved specific growth rates of largemouth bass,and significantly increased the height and width of intestinal villi and intestinal absorption area.Secondly,supplementation of low concentrations of nano iron through intraperitoneal injection significantly increased the activity of intestinal antioxidant enzymes catalase and total superoxide dismutase(CAT and T-SOD),enhanced the expression of antioxidant-related genes(sod1,nrf-2)and proteins(CAT;P<0.05),and decreased malondialdehyde(MDA)content,resulting in higher survival rates of Nocardia seriolae.Supplementation of 480 mg/kg compromised the immune and antioxidant capacity of juvenile largemouth bass,as evident by the increased expression of inflammatory and apoptotic genes,significant reduction in the expression of intestinal tight junction genes(zo-1,claudin1,claudin7;P<0.05),impairment of intestinal physical barriers,and exacerbation of oxidative stress levels.Through quadratic regression analysis,the optimal dietary supplementation level of nano iron for juvenile largemouth bass was determined to be approximately 80 mg/kg.In conclusion,moderate supplementation of nano iron appears to enhance the intestinal physical barrier,antioxidant capacity,and immune function of juvenile largemouth bass.展开更多
Competing endogenous RNAs(ceRNAs)are a novel epigenetic regulatory mechanism implicated in sex determination and differentiation in teleosts,which exhibit a diversity of sex-determining mechanisms.In this study,based ...Competing endogenous RNAs(ceRNAs)are a novel epigenetic regulatory mechanism implicated in sex determination and differentiation in teleosts,which exhibit a diversity of sex-determining mechanisms.In this study,based on whole transcriptome sequencing data,a ceRNA regulatory network composed of sex-inclined miRNAs(miR-456),lncRNAs(ASTR),circRNAs(circklhl29),and target mRNAs(spire1 and sox11)was uncovered in the gonadal tissues of Seriola dumerili,an oceanic species with lagged gonadal development.MiRNA-456 was identified as the core of the ceRNA regulatory network and suppressed the expression of spire1 and sox11,interacting with the ncRNAs including circklhl29 and ASTR.Co-localization of these sex-biased ncRNAs and mRNAs in the gonads suggests that this ceRNA network modulated the gonadal differentiation in both sexes of S.dumerili.In vivo injection in the gonads of S.dumerili suggested the miR-456 agonist could significantly inhibit expression of sox11 and spire1 in male testes,while significant regulatory effects of the miR-456 agonist and antagonist on sox11 and spire1 targets were not observed in female ovaries.The conserved binding sites for sequences of miR-456 and spire1/sox11 targets in various fish species were aligned and dual luciferase reporter gene experiments clarified the universality of the mechanism by which miR-456 bound to inhibit sox11/spire1 targets in large yellow croaker(Larimichthys crocea),half-smooth tongue sole(Cynoglossus semilaevis),and zebrafish(Danio rerio).These results further support the notion that ceRNA networks may be a universal regulatory system in teleosts despite their highly divergent sex regulation programs.展开更多
The aquaculture of yellowtail kingfish(Seriola lalandi)has expanded around the globe,including China.Genetic resource of Chinese S.lalandi urgently needs to be assessed for improving production.Here,we collected wild ...The aquaculture of yellowtail kingfish(Seriola lalandi)has expanded around the globe,including China.Genetic resource of Chinese S.lalandi urgently needs to be assessed for improving production.Here,we collected wild S.lalandi samples from the Bohai Sea,China and evaluated its genetic diversity based on 17,690 nuclear loci.A population from the Southern Ocean,Australia was used for comparison.The analyses showed that the Chinese and Australian S.lalandi formed two completely distinct clusters and there was no genetic introgression from the Australian S.lalandi into the Chinese S.lalandi population.The genetic diversity is slightly lower,but comparable in Chinese versus Australian yellowtail kingfish(Chinese population,expected heterozygosity:0.19,observed heterozygosity:0.19,nucleotide diversity:0.19±0.09;Australian population,expected heterozygosity:0.23,observed heterozygosity:0.22,nucleotide diversity:0.22±0.11).Overall,our results indicated that Chinese S.lalandi could be a potential subject for genetic breeding programs.We also investigated morphological characters and developed molecular markers for population identification.Comparison of meristic characters between the Chinese population and the Australian population revealed that main shape difference were in the number of dorsal spines,dorsal fin rays and the number of upper gill rakers.Geometric morphology based on eight landmarks also revealed significant difference between the two populations including the distance between the tip of snout to origin of pelvic fin and the distance between the tip of snout to origin of pectoral fin.These morphological characters can be used for easy identification of the Chinese S.lalandi.展开更多
We investigated movement pattern of commercial important fish around Offshore Wind Turbine(OWT)and neighboring habitats in Goto Islands.We tagged 55 individuals of 2 fish species and released around the OWT in 2017.(2...We investigated movement pattern of commercial important fish around Offshore Wind Turbine(OWT)and neighboring habitats in Goto Islands.We tagged 55 individuals of 2 fish species and released around the OWT in 2017.(27 Pagrus major from 1st Feb 2017 to 16th Mar 2017(winter),8 P.major and 20 Seriola quinqueradiata from 13th July 2017 to 13th Oct 2017(summer)).Acoustic receivers were deployed at OWT,south artificial reef(AS),trapnet(TN),north FAD(F1)and south FAD(F2)in winter and two more receivers were deployed at north artificial reef(AN)and natural reef(NR)in summer.We observed 12 P.major at OWT for a day after released in winter,majority of fish(13 individuals)were detected at TN.In summer,7 P.major were observed at OWT,1 visited TN within the day,but others disappeared.Ten S.quinqueradiata stayed at OWT longer than P.major after released and disappeared within 2 days.They mostly moved around AS,AN,NR.Residence time from K-M curve was 10 days for P.major in winter,a day in summer and 3 days for S.quinqueradiata.Both species showed low affinity for OWT as recorded low residency index despite the season.展开更多
Objective: To understand the effect of weaning time on the rearing performance of yellowtail amberjack Seriola lalandi dorsalis in the experimental condition. Methods: The same weaning protocol started on four differe...Objective: To understand the effect of weaning time on the rearing performance of yellowtail amberjack Seriola lalandi dorsalis in the experimental condition. Methods: The same weaning protocol started on four different days of post hatching (DPH), including 12 DPH (W12), 15 DPH (W15), 18 DPH (W18), and 21 DPH (W21), respectively. Growth, survival, and jaw malformation were used as the assessment criteria to evaluate the impact of weaning time on the performance of yellowtail amberjack larvae. Results: The highest specific growth rate was observed in W21 treatment, and the lowest specific growth rate was found in W12 treatment. The highest survival was achieved in W21 treatment, and the lowest survival was recorded in W12 treatment. With postponing the weaning started time, jaw malformation rate significantly reduced. At the end of this study, the highest malformation rate was observed in W12 treatment, and the malformation rate was not significantly different in W15, W18, and W21 treatment. Conclusions: Base on the results obtained in this study, we suggest that weaning of yellowtail amberjack larvae should be started from 15 DPH.展开更多
基金supported by the Guangdong Basic and Applied Basic Research Foundation(2024A1515012674,2024A1515010074)the Shenzhen Science and Technology Program(JCYJ20240813111703005,JCYJ20220530162006015)+1 种基金the China Postdoctoral Science Foundation(2023M733697)the Special Projects in Key Fields of Colleges and Universities in Guangdong Province(2023ZDZX4008).
文摘Nocardia seriolae alters macrophages to a“foamy”phenotype characterized by the accumulation of lipid droplets during infection to create a habitat that is ideally suited to its requirements.However,little is known about how exactly it does this.We extracted and identified specific lipids from N.seriolae,mycolic acids(NsMA),and confirmed they induced foam cells for granuloma formation in a snakehead(Channa argus)model.RNA-sequencing revealed that NsMA-induced foam cells were associated with Coronin-1A,which was further supported by Coronin-1A binding to NsMA as well as to the wild-type strain ZJ0503 rather than the NsMA-deficient strainΔfadD32.An in vitro assay revealed that the NsMA-induced Coronin-1A was responsible for inhibiting autophagy,whereas the inhibitory effect of autophagy was reversed in macrophages before receiving an anti-Coronin-1A antibody or mTOR inducer treatment.Meanwhile,the same results were also found by RNA interfering in vivo:silencing coronin-1A activated autophagy in NsMA-challenged snakehead,thereby alleviating the symptoms of granuloma formation with foam cell populations.Taken together,these results demonstrate that NsMA depends on the host's Coronin-1A to inhibit autophagy through the mTOR pathway,and thereby promotes cell foam for granuloma formation in N.seriolae-infected fish.
基金The Fund of Southern Marine Science and Engineering Guangdong Laboratory(Zhanjiang)under contract No.ZJW-2023-01the Guangdong Basic and Applied Basic Research Foundation under contract Nos 2023A1515010576 and 2024A1515012859the Marine Youth Talent Project of Zhanjiang under contract No.2023E0006.
文摘Sex-biased microRNAs(miRNAs)influence gonadal development in fish by directly targeting genes associated with estrogen production pathways.WW domain-binding protein 2(WBP2)functions as a crucial transcriptional co-activator of the estrogen and progesterone receptors(PGR).This study investigates the direct modulation of a sex-biased miR-133b on wbp2 and its regulatory role in gonadal development in fish,the greater amberjack(Seriola dumerili).Using dual-luciferase reporter assays,we demonstrate that wbp2 is a direct target of miR-133b,with miR-133b-3p binding to the 3'untranslated region(3'UTR)of wbp2.In vitro,miR-133b mimic significantly downregulate wbp2 expression,while the miR-133b inhibitor increase wbp2 levels.Consistently,in vivo,wbp2 expression is upregulated following antagomir-133b treatment and downregulated following agomir-133b.RNA fluorescence in situ hybridization(RNA-FISH)results reveal miR-133b and wbp2 co-localization in ovarian interstitial cells.Notably,phylogenetic analysis indicates that miR-133b-3p and wbp2 are highly conserved among bony fish species.Additionally,dual-luciferase assays in other bony fish species including Oreochromis niloticus and Danio rerio also confirm the targeting effect of miR-133b-3p on wbp2,suggesting that this regulatory mechanism is conserved across bony fish.This research provides a theoretical foundation for further exploration of non-coding RNA-mediated regulation in gonadal development in teleost.
基金Supported by NSFC (No. B9910030)Key Technologies Program of Fujian Province (No. 2000z080)
文摘Bacterin was prepared by formalin-inactivating the virulent strain of Vibrio hollisae isolated from diseased Seriola dumerili （amberjack） suffering from vibriosis. Healthy S. dumeriIi were vaccinated by respective procedures of intramuscular injection, immersion, and orally administration. Results of the three different vaccinations were compared. Blood was drawn from the vaccinated fish every 7 days, and the antibody titers and lysozyme activities of the sera were determined. The antibody titer of injected fish was 1：40 at 7 d, and reached its peak of 1：320 at 28 d, while the fish vaccinated by immersion and orally administration exhibited weak antibody responses, the antibody titres of 〈1：10, 1：20, 1：160 were observed at 7 d, 14 d, 35 d respectively. Compared with the control, the vaccinated fish exhibited significantly higher lysozyme activities （P〈0.05）. Upon challenge with virulent strain, the relative percent survival （RPS） of injected, immersed and oral administrated fish were 75%, 45%, and 40% respectively, and the injected fish showed significantly higher RPS than immersed and oral administrated fish. The results suggested that vaccination of S. dumerili by the injection would be the best strategy to prevent the vibriosis in S. dumerili farm.
基金the National Key Research and Development Program(Nos.2018YFD0901204 and 2019YFD0900503)the National Natural Science Foun-dation of China(No.31772829)+4 种基金the Laboratory for Ma-rine Fisheries Science and Food Production Processes,Qingdao National Laboratory for Marine Science and Technology,P.R.China(No.2017-3A01)Central Pub-lic-interest Scientific Institution Basal Research,CAFS&Key Laboratory of Sustainable Development of Marine Fisheries,Ministry of Agriculture and Rural Affairs,P.R.China(No.2019HY-XKQ01)the Central Public-interest Scientific Institution Basal Research Fund,YSFRI,CAFS(No.20603022021004)the Central Public-interest Scien-tific Institution Basal Research fund,CAFS(No.47)the China Agriculture Research System(No.CARS-47).
文摘Myogenic Regulatory Factors(MRFs)is involved in the muscle growth and differentiation.In this study,the cDNA sequence of yellowtail kingfish MRFs genes were cloned by rapid amplification of cDNA ends(RACE)method;then,the character-istics of these genes and the predicted protein sequences were analyzed by bioinformatics methods,the tissue and embryonic stages differential expression pattern were detected by the quantitative real-time PCR.Our results showed that the yellowtail kingfish(YTK)Myf5 cDNA has a full length of 951 bp,encoding 266 amino acids.The yellowtail kingfish Myf6 cDNA has a full length of 1105bp,encoding 250 amino acids.The proteins containα-helix,β-strand,and loops.The Neighbour-joining tree revealed that YTK Myf5 and Myf6 are closely related to Seriola dumerili.The yellowtail kingfish Myf5 and Myf6 gene expressed significantly higher in mus-cle than in other tissues(P<0.05).In addition,Myf5 and Myf6 in muscle was significantly expressed in 400g and 500 g fish but not in 50 g,suggesting that myogenic regulatory factors expression had a great relationship with the fish size.Our results also indicated that Myf5 and Myf6 have different functions during embryonic development,because Myf5 showed highest expression level at the neuroembryo period,but Myf6 had the highest expression level at embryo coverage yolk 70%stage.Myf5 gene showed highest ex-pression at 30 d of age,suggesting it played key roles in myogenic period.However,the Myf6 gene was significantly highly ex-pressed at 60 d,revealing this gene functioned in the later muscle formation period.
基金supported by the Natural Science Foundation of Sichuan Province,China(NO:2024NSFSC2069)also supported by the Freshwater Fish Innovation of Sichuan Province,China(NO.SCCXTD-2025-15).
文摘Nano-additives can improve the absorption and utilization of feed nutrients.In this study,we reported the effects of supplementary nano iron(0,30,60,120,240 and 480 mg/kg)on intestinal tract function of largemouth bass juveniles.Firstly,60 mg/kg of supplementary nano iron improved specific growth rates of largemouth bass,and significantly increased the height and width of intestinal villi and intestinal absorption area.Secondly,supplementation of low concentrations of nano iron through intraperitoneal injection significantly increased the activity of intestinal antioxidant enzymes catalase and total superoxide dismutase(CAT and T-SOD),enhanced the expression of antioxidant-related genes(sod1,nrf-2)and proteins(CAT;P<0.05),and decreased malondialdehyde(MDA)content,resulting in higher survival rates of Nocardia seriolae.Supplementation of 480 mg/kg compromised the immune and antioxidant capacity of juvenile largemouth bass,as evident by the increased expression of inflammatory and apoptotic genes,significant reduction in the expression of intestinal tight junction genes(zo-1,claudin1,claudin7;P<0.05),impairment of intestinal physical barriers,and exacerbation of oxidative stress levels.Through quadratic regression analysis,the optimal dietary supplementation level of nano iron for juvenile largemouth bass was determined to be approximately 80 mg/kg.In conclusion,moderate supplementation of nano iron appears to enhance the intestinal physical barrier,antioxidant capacity,and immune function of juvenile largemouth bass.
基金financed by the Fund of Southern Marine Science and EngineeringGuangdong Laboratory(Zhanjiang)(ZJW-2023-01)the Guangdong Basic and Applied Basic Research Foundation(2023A1515010576,20241515012859)the Youth Science and Technology Innovation Project in 2024(2023E0006).
文摘Competing endogenous RNAs(ceRNAs)are a novel epigenetic regulatory mechanism implicated in sex determination and differentiation in teleosts,which exhibit a diversity of sex-determining mechanisms.In this study,based on whole transcriptome sequencing data,a ceRNA regulatory network composed of sex-inclined miRNAs(miR-456),lncRNAs(ASTR),circRNAs(circklhl29),and target mRNAs(spire1 and sox11)was uncovered in the gonadal tissues of Seriola dumerili,an oceanic species with lagged gonadal development.MiRNA-456 was identified as the core of the ceRNA regulatory network and suppressed the expression of spire1 and sox11,interacting with the ncRNAs including circklhl29 and ASTR.Co-localization of these sex-biased ncRNAs and mRNAs in the gonads suggests that this ceRNA network modulated the gonadal differentiation in both sexes of S.dumerili.In vivo injection in the gonads of S.dumerili suggested the miR-456 agonist could significantly inhibit expression of sox11 and spire1 in male testes,while significant regulatory effects of the miR-456 agonist and antagonist on sox11 and spire1 targets were not observed in female ovaries.The conserved binding sites for sequences of miR-456 and spire1/sox11 targets in various fish species were aligned and dual luciferase reporter gene experiments clarified the universality of the mechanism by which miR-456 bound to inhibit sox11/spire1 targets in large yellow croaker(Larimichthys crocea),half-smooth tongue sole(Cynoglossus semilaevis),and zebrafish(Danio rerio).These results further support the notion that ceRNA networks may be a universal regulatory system in teleosts despite their highly divergent sex regulation programs.
基金supported by National Natural Science Fundation(31572657,31372563)Special Funds from the Administration of Ocean and Fisheries of Guangdong Province(A201512C003,2015-115)+2 种基金Special Fund for Science and Technology from Hubei Province(2015BBA228)Fund from Wuhan Science and Technology Bureau(2016020101010089)“Innovation and Strong Universities”Special Funds(KA170500G)from the Department of Education of Guangdong Province
文摘利用转录组技术对鰤诺卡氏菌(Nocardia seriolae)感染的乌鳢(Channa argus)进行分析与比较,以揭示可能的免疫机制,尤其在Toll样受体和Nod样受体的免疫反应.从乌鳢的头肾提取总RNA,采用Illumina Hi Seq TM2500进行测序和de novo转录组分析.乌鳢感染鰤诺卡氏菌后,对照组和试验组的clean reads分别是33 556 284(93.79%)和34 202 766(93.52%).利用Trinity软件对unigenes进行注释,从133 999条unigenes里,一共106319条unigenes得到注释,占79.34%;NR(NCBI non-redundant protein database)注释54 886(40.97%)条unigenes;39 795(29.69%)的Swiss-Prot注释;5 885(4.39%)条unigenes被KEGG(Kyoto encyclopedia of genes and genomes)注释到335条通路;5 753(4.29%)被注释到GO(Gene ontology)里.3 912条unigenes表达差异,其中1552条表达上调(39.67%)和2 360条表达下调(60.33%).Toll样受体和Nod样受体的免疫信号通路分别有28和13条差别表达基因发现有调制,12和3个上调,11和6个下调,5和4个unigenes没有显著的变化.经过转录组测序分析显示,乌鳢感染鰤诺卡氏菌后的转录复杂程度高,导致感染个体重诱导表达的基因产品的验证发现,尤其在非特异性免疫系统方面.
基金This work was supported by Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding.
文摘The aquaculture of yellowtail kingfish(Seriola lalandi)has expanded around the globe,including China.Genetic resource of Chinese S.lalandi urgently needs to be assessed for improving production.Here,we collected wild S.lalandi samples from the Bohai Sea,China and evaluated its genetic diversity based on 17,690 nuclear loci.A population from the Southern Ocean,Australia was used for comparison.The analyses showed that the Chinese and Australian S.lalandi formed two completely distinct clusters and there was no genetic introgression from the Australian S.lalandi into the Chinese S.lalandi population.The genetic diversity is slightly lower,but comparable in Chinese versus Australian yellowtail kingfish(Chinese population,expected heterozygosity:0.19,observed heterozygosity:0.19,nucleotide diversity:0.19±0.09;Australian population,expected heterozygosity:0.23,observed heterozygosity:0.22,nucleotide diversity:0.22±0.11).Overall,our results indicated that Chinese S.lalandi could be a potential subject for genetic breeding programs.We also investigated morphological characters and developed molecular markers for population identification.Comparison of meristic characters between the Chinese population and the Australian population revealed that main shape difference were in the number of dorsal spines,dorsal fin rays and the number of upper gill rakers.Geometric morphology based on eight landmarks also revealed significant difference between the two populations including the distance between the tip of snout to origin of pelvic fin and the distance between the tip of snout to origin of pectoral fin.These morphological characters can be used for easy identification of the Chinese S.lalandi.
文摘We investigated movement pattern of commercial important fish around Offshore Wind Turbine(OWT)and neighboring habitats in Goto Islands.We tagged 55 individuals of 2 fish species and released around the OWT in 2017.(27 Pagrus major from 1st Feb 2017 to 16th Mar 2017(winter),8 P.major and 20 Seriola quinqueradiata from 13th July 2017 to 13th Oct 2017(summer)).Acoustic receivers were deployed at OWT,south artificial reef(AS),trapnet(TN),north FAD(F1)and south FAD(F2)in winter and two more receivers were deployed at north artificial reef(AN)and natural reef(NR)in summer.We observed 12 P.major at OWT for a day after released in winter,majority of fish(13 individuals)were detected at TN.In summer,7 P.major were observed at OWT,1 visited TN within the day,but others disappeared.Ten S.quinqueradiata stayed at OWT longer than P.major after released and disappeared within 2 days.They mostly moved around AS,AN,NR.Residence time from K-M curve was 10 days for P.major in winter,a day in summer and 3 days for S.quinqueradiata.Both species showed low affinity for OWT as recorded low residency index despite the season.
文摘Objective: To understand the effect of weaning time on the rearing performance of yellowtail amberjack Seriola lalandi dorsalis in the experimental condition. Methods: The same weaning protocol started on four different days of post hatching (DPH), including 12 DPH (W12), 15 DPH (W15), 18 DPH (W18), and 21 DPH (W21), respectively. Growth, survival, and jaw malformation were used as the assessment criteria to evaluate the impact of weaning time on the performance of yellowtail amberjack larvae. Results: The highest specific growth rate was observed in W21 treatment, and the lowest specific growth rate was found in W12 treatment. The highest survival was achieved in W21 treatment, and the lowest survival was recorded in W12 treatment. With postponing the weaning started time, jaw malformation rate significantly reduced. At the end of this study, the highest malformation rate was observed in W12 treatment, and the malformation rate was not significantly different in W15, W18, and W21 treatment. Conclusions: Base on the results obtained in this study, we suggest that weaning of yellowtail amberjack larvae should be started from 15 DPH.
