The increasing trends in SoCs and SiPs technologies demand integration of large numbers of buses and metal tracks for interconnections. On-Chip SerDes Transceiver is a promising solution which can reduce the number of...The increasing trends in SoCs and SiPs technologies demand integration of large numbers of buses and metal tracks for interconnections. On-Chip SerDes Transceiver is a promising solution which can reduce the number of interconnects and offers remarkable benefits in context with power consumption, area congestion and crosstalk. This paper reports a design of a new Serializer and Deserializer architecture for basic functional operations of serialization and deserialization used in On-Chip SerDes Transceiver. This architecture employs a design technique which samples input on both edges of clock. The main advantage of this technique which is input is sampled with lower clock (half the original rate) and is distributed for the same functional throughput, which results in power savings in the clock distribution network. This proposed Serializer and Deserializer architecture is designed using UMC 180 nm CMOS technology and simulation is done using Cadence Spectre simulator with a supply voltage of 1.8 V. The present design is compared with the earlier published similar works and improvements are obtained in terms of power consumption and area as shown in Tables 1-3 respectively. This design also helps the designer for solving crosstalk issues.展开更多
The common design of serial civil aircraft, an important strategy of modern civil aircraft research and develop-ment, minimizes the whole life cycle cost of civil aircraft through asset reuse and resource sharing. How...The common design of serial civil aircraft, an important strategy of modern civil aircraft research and develop-ment, minimizes the whole life cycle cost of civil aircraft through asset reuse and resource sharing. However, the existing estimating model for the R&D cost of civil aircraft ignores the effects of common design, so the value estimated by estimating derivative models is significantly inconsistent with the actual one. To solve this problem, a novel assessment method for civil aircraft commonality indicators is developed based on fuzzy set in the present study, exploiting the attributes and structural parameters of the aircraft to be assessed as input to determine the degree of membership that pertains to the commonality sub-interval as the commonality indicator.Then the BP(Back Propagation) neural network algorithm is adopted to establish the relationship between the common index and the decrease rate of the R&D cost of derivative models. The model employs over a dozen typical civil aircraft models(e.g., Boeing, Airbus, and Bombardier) as the sample data for network learning training to build a mature neural network model for estimating the R&D cost of novel derivative models. As revealed from the comparative analysis on the calculated results of the samples, the estimated results of the model given the effects of commonality in the present study exhibit higher estimation accuracy and value for future work.展开更多
In order to apply the knowledge based engineering to solve the low efficiency of serialized design,by analyzing the top-down design characteristics,a composite representation method for the design knowledge is propose...In order to apply the knowledge based engineering to solve the low efficiency of serialized design,by analyzing the top-down design characteristics,a composite representation method for the design knowledge is proposed.The object-knowledge is expressed by framework,and the construction principles of the framework are given,the design knowledge element is used to express the process-knowledge.Then the knowledge reasoning model for design decision-making is established.Finally,a case shows that the proposed method and model are effective.展开更多
This editorial is a commentary on the case report by Furuya et al focusing on the challenging diagnosis of early pancreatic adenocarcinoma and new tools for an earlier diagnosis.Currently,pancreatic cancer still has a...This editorial is a commentary on the case report by Furuya et al focusing on the challenging diagnosis of early pancreatic adenocarcinoma and new tools for an earlier diagnosis.Currently,pancreatic cancer still has a poor prognosis,mainly due to late diagnosis in an advanced stage.Two main precancerous routes have been identified as pathways to pancreatic adenocarcinoma:The first encompasses a large group of mucinous cystic lesions:intraductal papillary mucinous neoplasm and mucinous cystic neoplasm,and the second is pancreatic intraepithelial neoplasia.In the last decade the focus of research has been to identify high-risk patients,using advanced imaging techniques(magnetic resonance cholangiopancreatography or endoscopic ultrasonography)which could be helpful in finding“indirect signs”of early stage pancreatic lesions.Nevertheless,the survival rate still remains poor,and alternative screening methods are under investigation.Endoscopic retrograde cholangiopancreatography followed by serial pancreatic juice aspiration cytology could be a promising tool for identifying precursor lesions such as intraductal papillary mucinous neoplasm,but confirming data are still needed to validate its role.Probably a combination of cross-sectional imaging,endoscopic techniques(old and new ones)and genetic and biological biomarkers also in pancreatic juice)could be the best solution to reach an early diagnosis.Biomarkers could help to predict and follow the progression of early pancreatic lesions.However,further studies are needed to validate their diagnostic reliability and to establish diagnostic algorithms to improve prognosis and survival in patients with pancreatic cancer.展开更多
This study investigated the relationship between college students’awe and depression,along with the mediating roles of future self-continuity and presence of meaning.891 Chinese college students(570 female;mean age 1...This study investigated the relationship between college students’awe and depression,along with the mediating roles of future self-continuity and presence of meaning.891 Chinese college students(570 female;mean age 18.59;SD 1.34)from one university completed four surveys:Dispositional Awe Subscale,Future self-continuity Scale,Meaning in Life Scale and Depression Scale.Using structural equation modelling and the bootstrap method,the results delineated that awe negatively related to depression,and future self-continuity and presence of meaning had a serial mediation effect,reducing depression.The study implies educational institutions should foster a positive mental health education environment,urging students to develop positive traits,enhancing well-being and resilience,and facilitating psychological development.展开更多
Fractures are critical to subsurface activities such as oil and gas extraction,geothermal energy production,and carbon storage.Hydraulic fracturing,a technique that enhances fluid production,creates complex fracture n...Fractures are critical to subsurface activities such as oil and gas extraction,geothermal energy production,and carbon storage.Hydraulic fracturing,a technique that enhances fluid production,creates complex fracture networks within rock formations containing natural discontinuities.Accurately distinguishing between hydraulically induced fractures and pre-existing discontinuities is essential for understanding hydraulic fracture mechanisms.However,this remains challenging due to the interconnected nature of fractures in three-dimensional(3D)space.Manual segmentation,while adaptive,is both labor-intensive and subjective,making it impractical for large-scale 3D datasets.This study introduces a deep learning-based progressive cross-sectional segmentation method to automate the classification of 3D fracture volumes.The proposed method was applied to a 3D hydraulic fracture network in a Montney cube sample,successfully segmenting natural fractures,parted bedding planes,and hydraulic fractures with minimal user intervention.The automated approach achieves a 99.6%reduction in manual image processing workload while maintaining high segmentation accuracy,with test accuracy exceeding 98%and F1-score over 84%.This approach generalizes well to Brazilian disc samples with different fracture patterns,achieving consistently high accuracy in distinguishing between bedding and non-bedding fractures.This automated fracture segmentation method offers an effective tool for enhanced quantitative characterization of fracture networks,which would contribute to a deeper understanding of hydraulic fracturing processes.展开更多
This paper introduces an intelligent garbage-handling trolley model based on an STM32 single chip microcomputer as the control core.The device is driven by four independent motors to achieve automatic tracking,automat...This paper introduces an intelligent garbage-handling trolley model based on an STM32 single chip microcomputer as the control core.The device is driven by four independent motors to achieve automatic tracking,automatic obstacle avoidance,and fixed-point docking.Using external execution structure to realize the car without the use of a mechanical arm,complete garbage collection,storage,and uninstall function.On this basis,the type of garbage is marked by color,and the color recognition sensor is applied to realize the type recognition after garbage collection and put into the corresponding unloading point,to realize its intelligent classification function.It can automatically complete the established task autonomously.展开更多
The first in vivo measurements of serial sarcomere number in human muscles before and after eccentric strength training have just been published and the results will interest anyone involved with sport or health:Train...The first in vivo measurements of serial sarcomere number in human muscles before and after eccentric strength training have just been published and the results will interest anyone involved with sport or health:Training the hamstrings 3 times per week for 9 weeks with the Nordic hamstring exercise was found to increase the number of sarcomeres in series in the biceps femoris long head(BFlh)by≥25%.1 In this commentary,we highlight an additional,paradoxical finding,which was not discussed by the authors;namely that the substantial serial sarcomere addition must have been accompanied by a subtraction of sarcomeres in parallel to match the relatively small increase in muscle volume after training.展开更多
Sarcomerogenesis,the addition of serial sarcomeres in skeletal muscle myofibrils and fibres,is a natural occurrence during growth and maturation of animals,including humans.However,the detailed mechanisms that allow f...Sarcomerogenesis,the addition of serial sarcomeres in skeletal muscle myofibrils and fibres,is a natural occurrence during growth and maturation of animals,including humans.However,the detailed mechanisms that allow for sarcomerogenesis are not fully understood.In some diseases,such as cerebral palsy in children,sarcomerogenesis appears to be inhibited or at least reduced,1,2 often causing severe restrictions in muscle and joint function.展开更多
Contemporary Social Sciences is an English-language journal founded by Sichuan Academy of Social Sciences(SASS)in March 2016.It is China’s first and only English academic journal established by a local academy of soc...Contemporary Social Sciences is an English-language journal founded by Sichuan Academy of Social Sciences(SASS)in March 2016.It is China’s first and only English academic journal established by a local academy of social sciences,as well as Sichuan’s only English academic journal in humanities and social sciences with a CN serial number.展开更多
Rapidly obtaining spatial distribution maps of secondary disasters triggered by strong earthquakes is crucial for understanding the disaster-causing processes in the earthquake hazard chain and formulating effective e...Rapidly obtaining spatial distribution maps of secondary disasters triggered by strong earthquakes is crucial for understanding the disaster-causing processes in the earthquake hazard chain and formulating effective emergency response measures and post-disaster reconstruction plans.On April 3,2024,a M_(W)7.4 earthquake struck offshore east of Hualien,Taiwan,China,which triggered numerous coseismic landslides in bedrock mountain regions and severe soil liquefaction in coastal areas,resulting in significant economic losses.This study utilized postearthquake emergency data from China's high-resolution optical satellite imagery and applied visual interpretation method to establish a partial database of secondary disasters triggered by the 2024 Hualien earthquake.A total of 5348 coseismic landslides were identified,which were primarily distributed along the eastern slopes of the Central Mountain Range watersheds.In high mountain valleys,these landslides mainly manifest as localized bedrock collapses or slope debris flows,causing extensive damage to highways and tourism facilities.Their distribution partially overlaps with the landslide concentration zones triggered by the 1999 Chi-Chi earthquake.Additionally,6040 soil liquefaction events were interpreted,predominantly in the Hualien Port area and the lowland valleys of the Hualien River and concentrated within the IX-intensity zone.Widespread surface subsidence and sand ejections characterized soil liquefaction.Verified against local field investigation data in Taiwan,rapid imaging through post-earthquake remote sensing data can effectively assess the distribution of coseismic landslides and soil liquefaction within high-intensity zones.This study provides efficient and reliable data for earthquake disaster response.Moreover,the results are critical for seismic disaster mitigation in high mountain valleys and coastal lowlands.展开更多
BACKGROUND Mesenchymal stem cells(MSCs)have been extensively studied for therapeutic potential,due to their regenerative and immunomodulatory properties.Serial passage and stress factors may affect the biological char...BACKGROUND Mesenchymal stem cells(MSCs)have been extensively studied for therapeutic potential,due to their regenerative and immunomodulatory properties.Serial passage and stress factors may affect the biological characteristics of MSCs,but the details of these effects have not been recognized yet.AIM To investigate the effects of stress factors(high glucose and severe hypoxia)on the biological characteristics of MSCs at different passages,in order to optimize the therapeutic applications of MSCs.METHODS In this study,we investigated the impact of two stress conditions;severe hypoxia and high glucose on human adipose-tissue derived MSCs(hAD-MSCs)at passages 6(P6),P8,and P10.Proliferation,senescence and apoptosis were evaluated measuring WST-1,senescence-associated beta-galactosidase,and annexin V,respectively.RESULTS Cells at P6 showed decreased proliferation and increased apoptosis under conditions of high glucose and hypoxia compared to control,while the extent of senescence did not change significantly under stress conditions.At P8 hAD-MSCs cultured in stress conditions had a significant decrease in proliferation and apoptosis and a significant increase in senescence compared to counterpart cells at P6.Cells cultured in high glucose at P10 had lower proliferation and higher senescence than their counterparts in the previous passage,while no change in apoptosis was observed.On the other hand,MSCs cultured under hypoxia showed decreased senescence,increased apoptosis and no significant change in proliferation when compared to the same conditions at P8.CONCLUSION These results indicate that stress factors had distinct effects on the biological processes of MSCs at different passages,and suggest that senescence may be a protective mechanism for MSCs to survive under stress conditions at higher passage numbers.展开更多
The study investigates long-term changes in annual and seasonal rainfall patterns in the Indira Sagar Region of Madhya Pradesh, India, from 1901 to 2010. Agriculture sustainability, food supply, natural resource devel...The study investigates long-term changes in annual and seasonal rainfall patterns in the Indira Sagar Region of Madhya Pradesh, India, from 1901 to 2010. Agriculture sustainability, food supply, natural resource development, and hydropower system reliability in the region rely heavily on monsoon rainfall. Monthly rainfall data from three stations (East Nimar, Barwani, and West Nimar) were analyzed. Initially, the pre-whitening method was applied to eliminate serial correlation effects from the rainfall data series. Subsequently, statistical trends in annual and seasonal rainfall were assessed using both parametric (student-t test) and non-parametric tests [Mann-Kendall, Sen’s slope estimator, and Cumulative Sum (CUSUM)]. The magnitude of the rainfall trend was determined using Theil-Sen’s slope estimator. Spatial analysis of the Mann-Kendall test on an annual basis revealed a statistically insignificant decreasing trend for Barwani and East Nimar and an increasing trend for West Nimar. On a seasonal basis, the monsoon season contributes a significant percentage (88.33%) to the total annual rainfall. The CUSUM test results indicated a shift change detection in annual rainfall data for Barwani in 1997, while shifts were observed in West and East Nimar stations in 1929. These findings offer valuable insights into regional rainfall behavior, aiding in the planning and management of water resources and ecological systems.展开更多
We utilized Raspberry Pi 4B to develop a microbial monitoring system to simplify the microbial image-capturing process and facilitate the informatization of microbial observation results.The Raspberry Pi 4B firmware,d...We utilized Raspberry Pi 4B to develop a microbial monitoring system to simplify the microbial image-capturing process and facilitate the informatization of microbial observation results.The Raspberry Pi 4B firmware,developed under Python on the Linux platform,achieves sum verification of serial data,file upload based on TCP protocol,control of sequence light source and light valve,real-time self-test based on multithreading,and an experiment-oriented file management method.The system demonstrated improved code logic,scheduling,exception handling,and code readability.展开更多
This paper presents a distributed arithmetic(DA)architecture that can efficiently implement finite impulse response(FIR)filters for biomedical signal processor applications.FIR filter design is more efficient when it ...This paper presents a distributed arithmetic(DA)architecture that can efficiently implement finite impulse response(FIR)filters for biomedical signal processor applications.FIR filter design is more efficient when it uses a look-up table(LUT)-based technique rather than a serial one.The design’s performance and efficiency can be improved by using segmented memory banks as well as memory lookup for multiply operation.Verilog HDL is used to model the proposed design,and Synopsys Design Compiler tool is used for synthesis.The FIR filter architecture utilizing DA results in a 24.82%reduction in total power compared with the serial FIR structure.展开更多
文摘The increasing trends in SoCs and SiPs technologies demand integration of large numbers of buses and metal tracks for interconnections. On-Chip SerDes Transceiver is a promising solution which can reduce the number of interconnects and offers remarkable benefits in context with power consumption, area congestion and crosstalk. This paper reports a design of a new Serializer and Deserializer architecture for basic functional operations of serialization and deserialization used in On-Chip SerDes Transceiver. This architecture employs a design technique which samples input on both edges of clock. The main advantage of this technique which is input is sampled with lower clock (half the original rate) and is distributed for the same functional throughput, which results in power savings in the clock distribution network. This proposed Serializer and Deserializer architecture is designed using UMC 180 nm CMOS technology and simulation is done using Cadence Spectre simulator with a supply voltage of 1.8 V. The present design is compared with the earlier published similar works and improvements are obtained in terms of power consumption and area as shown in Tables 1-3 respectively. This design also helps the designer for solving crosstalk issues.
文摘The common design of serial civil aircraft, an important strategy of modern civil aircraft research and develop-ment, minimizes the whole life cycle cost of civil aircraft through asset reuse and resource sharing. However, the existing estimating model for the R&D cost of civil aircraft ignores the effects of common design, so the value estimated by estimating derivative models is significantly inconsistent with the actual one. To solve this problem, a novel assessment method for civil aircraft commonality indicators is developed based on fuzzy set in the present study, exploiting the attributes and structural parameters of the aircraft to be assessed as input to determine the degree of membership that pertains to the commonality sub-interval as the commonality indicator.Then the BP(Back Propagation) neural network algorithm is adopted to establish the relationship between the common index and the decrease rate of the R&D cost of derivative models. The model employs over a dozen typical civil aircraft models(e.g., Boeing, Airbus, and Bombardier) as the sample data for network learning training to build a mature neural network model for estimating the R&D cost of novel derivative models. As revealed from the comparative analysis on the calculated results of the samples, the estimated results of the model given the effects of commonality in the present study exhibit higher estimation accuracy and value for future work.
基金the outsourcing project of the AECC Sichuan Gas Turbine Establishment and the science and technology project of Sichuan province(grant number 2019YFG0312)。
文摘In order to apply the knowledge based engineering to solve the low efficiency of serialized design,by analyzing the top-down design characteristics,a composite representation method for the design knowledge is proposed.The object-knowledge is expressed by framework,and the construction principles of the framework are given,the design knowledge element is used to express the process-knowledge.Then the knowledge reasoning model for design decision-making is established.Finally,a case shows that the proposed method and model are effective.
文摘This editorial is a commentary on the case report by Furuya et al focusing on the challenging diagnosis of early pancreatic adenocarcinoma and new tools for an earlier diagnosis.Currently,pancreatic cancer still has a poor prognosis,mainly due to late diagnosis in an advanced stage.Two main precancerous routes have been identified as pathways to pancreatic adenocarcinoma:The first encompasses a large group of mucinous cystic lesions:intraductal papillary mucinous neoplasm and mucinous cystic neoplasm,and the second is pancreatic intraepithelial neoplasia.In the last decade the focus of research has been to identify high-risk patients,using advanced imaging techniques(magnetic resonance cholangiopancreatography or endoscopic ultrasonography)which could be helpful in finding“indirect signs”of early stage pancreatic lesions.Nevertheless,the survival rate still remains poor,and alternative screening methods are under investigation.Endoscopic retrograde cholangiopancreatography followed by serial pancreatic juice aspiration cytology could be a promising tool for identifying precursor lesions such as intraductal papillary mucinous neoplasm,but confirming data are still needed to validate its role.Probably a combination of cross-sectional imaging,endoscopic techniques(old and new ones)and genetic and biological biomarkers also in pancreatic juice)could be the best solution to reach an early diagnosis.Biomarkers could help to predict and follow the progression of early pancreatic lesions.However,further studies are needed to validate their diagnostic reliability and to establish diagnostic algorithms to improve prognosis and survival in patients with pancreatic cancer.
基金supported by the Fundamental Research Funds for the Central Universities,and the Research Funds of Civil Aviation University of China,No.3122024012.
文摘This study investigated the relationship between college students’awe and depression,along with the mediating roles of future self-continuity and presence of meaning.891 Chinese college students(570 female;mean age 18.59;SD 1.34)from one university completed four surveys:Dispositional Awe Subscale,Future self-continuity Scale,Meaning in Life Scale and Depression Scale.Using structural equation modelling and the bootstrap method,the results delineated that awe negatively related to depression,and future self-continuity and presence of meaning had a serial mediation effect,reducing depression.The study implies educational institutions should foster a positive mental health education environment,urging students to develop positive traits,enhancing well-being and resilience,and facilitating psychological development.
基金supported through the Natural Sciences and Engineering Research Council of Canada(NSERC)Discovery Grants 341275,CRDPJ 543894-19NSERC/Energi Simulation Industrial Research Chair program.
文摘Fractures are critical to subsurface activities such as oil and gas extraction,geothermal energy production,and carbon storage.Hydraulic fracturing,a technique that enhances fluid production,creates complex fracture networks within rock formations containing natural discontinuities.Accurately distinguishing between hydraulically induced fractures and pre-existing discontinuities is essential for understanding hydraulic fracture mechanisms.However,this remains challenging due to the interconnected nature of fractures in three-dimensional(3D)space.Manual segmentation,while adaptive,is both labor-intensive and subjective,making it impractical for large-scale 3D datasets.This study introduces a deep learning-based progressive cross-sectional segmentation method to automate the classification of 3D fracture volumes.The proposed method was applied to a 3D hydraulic fracture network in a Montney cube sample,successfully segmenting natural fractures,parted bedding planes,and hydraulic fractures with minimal user intervention.The automated approach achieves a 99.6%reduction in manual image processing workload while maintaining high segmentation accuracy,with test accuracy exceeding 98%and F1-score over 84%.This approach generalizes well to Brazilian disc samples with different fracture patterns,achieving consistently high accuracy in distinguishing between bedding and non-bedding fractures.This automated fracture segmentation method offers an effective tool for enhanced quantitative characterization of fracture networks,which would contribute to a deeper understanding of hydraulic fracturing processes.
文摘This paper introduces an intelligent garbage-handling trolley model based on an STM32 single chip microcomputer as the control core.The device is driven by four independent motors to achieve automatic tracking,automatic obstacle avoidance,and fixed-point docking.Using external execution structure to realize the car without the use of a mechanical arm,complete garbage collection,storage,and uninstall function.On this basis,the type of garbage is marked by color,and the color recognition sensor is applied to realize the type recognition after garbage collection and put into the corresponding unloading point,to realize its intelligent classification function.It can automatically complete the established task autonomously.
文摘The first in vivo measurements of serial sarcomere number in human muscles before and after eccentric strength training have just been published and the results will interest anyone involved with sport or health:Training the hamstrings 3 times per week for 9 weeks with the Nordic hamstring exercise was found to increase the number of sarcomeres in series in the biceps femoris long head(BFlh)by≥25%.1 In this commentary,we highlight an additional,paradoxical finding,which was not discussed by the authors;namely that the substantial serial sarcomere addition must have been accompanied by a subtraction of sarcomeres in parallel to match the relatively small increase in muscle volume after training.
文摘Sarcomerogenesis,the addition of serial sarcomeres in skeletal muscle myofibrils and fibres,is a natural occurrence during growth and maturation of animals,including humans.However,the detailed mechanisms that allow for sarcomerogenesis are not fully understood.In some diseases,such as cerebral palsy in children,sarcomerogenesis appears to be inhibited or at least reduced,1,2 often causing severe restrictions in muscle and joint function.
文摘Contemporary Social Sciences is an English-language journal founded by Sichuan Academy of Social Sciences(SASS)in March 2016.It is China’s first and only English academic journal established by a local academy of social sciences,as well as Sichuan’s only English academic journal in humanities and social sciences with a CN serial number.
基金funded by the Basic Research program from the Institute of Earthquake Forecasting,China Earthquake Administration(Grant No.CEAIEF20240302)the National Natural Science Foundation of China(Grant Nos.42072248)the National Key Research and Development Program of China(Grant Nos.2021YFC3000600 and 2019YFE0108900)。
文摘Rapidly obtaining spatial distribution maps of secondary disasters triggered by strong earthquakes is crucial for understanding the disaster-causing processes in the earthquake hazard chain and formulating effective emergency response measures and post-disaster reconstruction plans.On April 3,2024,a M_(W)7.4 earthquake struck offshore east of Hualien,Taiwan,China,which triggered numerous coseismic landslides in bedrock mountain regions and severe soil liquefaction in coastal areas,resulting in significant economic losses.This study utilized postearthquake emergency data from China's high-resolution optical satellite imagery and applied visual interpretation method to establish a partial database of secondary disasters triggered by the 2024 Hualien earthquake.A total of 5348 coseismic landslides were identified,which were primarily distributed along the eastern slopes of the Central Mountain Range watersheds.In high mountain valleys,these landslides mainly manifest as localized bedrock collapses or slope debris flows,causing extensive damage to highways and tourism facilities.Their distribution partially overlaps with the landslide concentration zones triggered by the 1999 Chi-Chi earthquake.Additionally,6040 soil liquefaction events were interpreted,predominantly in the Hualien Port area and the lowland valleys of the Hualien River and concentrated within the IX-intensity zone.Widespread surface subsidence and sand ejections characterized soil liquefaction.Verified against local field investigation data in Taiwan,rapid imaging through post-earthquake remote sensing data can effectively assess the distribution of coseismic landslides and soil liquefaction within high-intensity zones.This study provides efficient and reliable data for earthquake disaster response.Moreover,the results are critical for seismic disaster mitigation in high mountain valleys and coastal lowlands.
基金Supported by the Deanship of Scientific Research,Yarmouk University,Jordan,No.73/2022.
文摘BACKGROUND Mesenchymal stem cells(MSCs)have been extensively studied for therapeutic potential,due to their regenerative and immunomodulatory properties.Serial passage and stress factors may affect the biological characteristics of MSCs,but the details of these effects have not been recognized yet.AIM To investigate the effects of stress factors(high glucose and severe hypoxia)on the biological characteristics of MSCs at different passages,in order to optimize the therapeutic applications of MSCs.METHODS In this study,we investigated the impact of two stress conditions;severe hypoxia and high glucose on human adipose-tissue derived MSCs(hAD-MSCs)at passages 6(P6),P8,and P10.Proliferation,senescence and apoptosis were evaluated measuring WST-1,senescence-associated beta-galactosidase,and annexin V,respectively.RESULTS Cells at P6 showed decreased proliferation and increased apoptosis under conditions of high glucose and hypoxia compared to control,while the extent of senescence did not change significantly under stress conditions.At P8 hAD-MSCs cultured in stress conditions had a significant decrease in proliferation and apoptosis and a significant increase in senescence compared to counterpart cells at P6.Cells cultured in high glucose at P10 had lower proliferation and higher senescence than their counterparts in the previous passage,while no change in apoptosis was observed.On the other hand,MSCs cultured under hypoxia showed decreased senescence,increased apoptosis and no significant change in proliferation when compared to the same conditions at P8.CONCLUSION These results indicate that stress factors had distinct effects on the biological processes of MSCs at different passages,and suggest that senescence may be a protective mechanism for MSCs to survive under stress conditions at higher passage numbers.
文摘The study investigates long-term changes in annual and seasonal rainfall patterns in the Indira Sagar Region of Madhya Pradesh, India, from 1901 to 2010. Agriculture sustainability, food supply, natural resource development, and hydropower system reliability in the region rely heavily on monsoon rainfall. Monthly rainfall data from three stations (East Nimar, Barwani, and West Nimar) were analyzed. Initially, the pre-whitening method was applied to eliminate serial correlation effects from the rainfall data series. Subsequently, statistical trends in annual and seasonal rainfall were assessed using both parametric (student-t test) and non-parametric tests [Mann-Kendall, Sen’s slope estimator, and Cumulative Sum (CUSUM)]. The magnitude of the rainfall trend was determined using Theil-Sen’s slope estimator. Spatial analysis of the Mann-Kendall test on an annual basis revealed a statistically insignificant decreasing trend for Barwani and East Nimar and an increasing trend for West Nimar. On a seasonal basis, the monsoon season contributes a significant percentage (88.33%) to the total annual rainfall. The CUSUM test results indicated a shift change detection in annual rainfall data for Barwani in 1997, while shifts were observed in West and East Nimar stations in 1929. These findings offer valuable insights into regional rainfall behavior, aiding in the planning and management of water resources and ecological systems.
文摘We utilized Raspberry Pi 4B to develop a microbial monitoring system to simplify the microbial image-capturing process and facilitate the informatization of microbial observation results.The Raspberry Pi 4B firmware,developed under Python on the Linux platform,achieves sum verification of serial data,file upload based on TCP protocol,control of sequence light source and light valve,real-time self-test based on multithreading,and an experiment-oriented file management method.The system demonstrated improved code logic,scheduling,exception handling,and code readability.
文摘This paper presents a distributed arithmetic(DA)architecture that can efficiently implement finite impulse response(FIR)filters for biomedical signal processor applications.FIR filter design is more efficient when it uses a look-up table(LUT)-based technique rather than a serial one.The design’s performance and efficiency can be improved by using segmented memory banks as well as memory lookup for multiply operation.Verilog HDL is used to model the proposed design,and Synopsys Design Compiler tool is used for synthesis.The FIR filter architecture utilizing DA results in a 24.82%reduction in total power compared with the serial FIR structure.
