The vaginal microbiome is critical for the reproductive health of women,yet the differential impacts exerted by the host and by ambient environmental variables on the vaginal microbiome remain largely unknown.Here,we ...The vaginal microbiome is critical for the reproductive health of women,yet the differential impacts exerted by the host and by ambient environmental variables on the vaginal microbiome remain largely unknown.Here,we conducted a comprehensive cross-sectional study of the relationships between the vaginal microbiome and 81 matched host and environmental variables across 6755 Chinese women.By 16S rRNA sequencing,we identified four core vaginal microbiota with a prevalence of over 90%and a total median abundance of 98.8%.Twenty-four variables,including physiology,lifestyle behaviors,gynecologic history,social and environmental information,were found associated with the microbiome composition,of which bacterial vaginosis(BV)showed the largest effect size.Age was among the strongest explanatory variables and the vaginal microbiome dynamically succeeded with increasing age,especially with a composition turning point at the age of 45.Our mediation analyses indicated that the effects of age on the microbiome could be mediated by variables such as parity number and lifestyles.We further classified the vaginal microbiomes of the population into 13“Vagitypes”.Women with Lactobacillus iners-and Lactobacillus jensenii-dominated Vagitypes had significantly higher live birth rate than those with Vagitype dominated by Fannyhessea vaginae(53.40%,59.09%vs 21.43%;OR[95%CI]:3.62[1.12-14.87],5.39[1.27-27.36];P=0.031,P=0.021).This study provides a comprehensive overview of the associations between identified variables and the vaginal microbiome,representing an important step toward understanding of environment-microbe-host interactions.展开更多
Objective:In this study,we aimed to identify the genes involved in leaf margin serration in Perilla frutescens.P.frutescens(Family:Lamiaceae)is widely grown in Asian countries.Perilla leaf is the medicinal part stipul...Objective:In this study,we aimed to identify the genes involved in leaf margin serration in Perilla frutescens.P.frutescens(Family:Lamiaceae)is widely grown in Asian countries.Perilla leaf is the medicinal part stipulated in the Chinese Pharmacopoeia.There are mainly two types of perilla leaves:one with serrated leaf margin which is the phenotype described in the pharmacopoeia and the other with smooth leaf margin.Methods:Transcriptome sequencing,co-expression analysis,and qRT-PCR analysis of six perilla tissues sampled from two different phenotypes(serrated and smooth leaves)were performed.Results:Forty-three differentially expressed genes(DEGs),which may potentially regulate leaf shape,were identified through de novo transcriptome sequencing between the two groups.Genes involved in leaf shape regulation were identified.Simultaneously,we validated five DEGs by qRT-PCR,and the results were consistent with the transcriptome data.In addition,1186 transcription factors(TFs)belonging to 45 TF families were identified.Moreover,the co-expression network of DEGs was constructed.Conclusion:The study identified the key genes that control leaf shape by comparing the transcriptomes.Our findings also provide basic data for further exploring P.frutescens,which can help study the mechanism of leaf shape development and molecular breeding.展开更多
基金funded by the National Key Research and Development Program of China 2021YFC2700400 and 2021YFC2700701 to H.Z.and S.G.Z.the Basic Science Center Program of NSFC 31988101,Shandong Provincial Key Research and Development Program 2020ZLYS02+4 种基金the Innovative Research Team of Highlevel Local Universities in Shanghai SHSMU-ZLCX20210200,to Z.-J.C.the National Natural Science Foundation of China 82421004,82192874,31871509,82071606,and 82071122 to H.Z.,S.G.Z.and Q.F.the National High-level Young Scientist Project Foundation(2019)and the Excellent Young Scientist Foundation of Shandong Province ZR202102230369 to Q.F.the Taishan Scholars Program of Shandong Province ts20190988 to H.Z.the Fundamental Research Funds of Shandong University 2023QNTDO04 to S.G.Z.
文摘The vaginal microbiome is critical for the reproductive health of women,yet the differential impacts exerted by the host and by ambient environmental variables on the vaginal microbiome remain largely unknown.Here,we conducted a comprehensive cross-sectional study of the relationships between the vaginal microbiome and 81 matched host and environmental variables across 6755 Chinese women.By 16S rRNA sequencing,we identified four core vaginal microbiota with a prevalence of over 90%and a total median abundance of 98.8%.Twenty-four variables,including physiology,lifestyle behaviors,gynecologic history,social and environmental information,were found associated with the microbiome composition,of which bacterial vaginosis(BV)showed the largest effect size.Age was among the strongest explanatory variables and the vaginal microbiome dynamically succeeded with increasing age,especially with a composition turning point at the age of 45.Our mediation analyses indicated that the effects of age on the microbiome could be mediated by variables such as parity number and lifestyles.We further classified the vaginal microbiomes of the population into 13“Vagitypes”.Women with Lactobacillus iners-and Lactobacillus jensenii-dominated Vagitypes had significantly higher live birth rate than those with Vagitype dominated by Fannyhessea vaginae(53.40%,59.09%vs 21.43%;OR[95%CI]:3.62[1.12-14.87],5.39[1.27-27.36];P=0.031,P=0.021).This study provides a comprehensive overview of the associations between identified variables and the vaginal microbiome,representing an important step toward understanding of environment-microbe-host interactions.
基金This work was funded by National Natural Science Foundation for regional fund(31860391)Guizhou Province Science and Technology Plan Project(Qian science platform[2019]5656)National Major Project for Breeding New Transgenic Varieties(2016ZX08010-003).
文摘Objective:In this study,we aimed to identify the genes involved in leaf margin serration in Perilla frutescens.P.frutescens(Family:Lamiaceae)is widely grown in Asian countries.Perilla leaf is the medicinal part stipulated in the Chinese Pharmacopoeia.There are mainly two types of perilla leaves:one with serrated leaf margin which is the phenotype described in the pharmacopoeia and the other with smooth leaf margin.Methods:Transcriptome sequencing,co-expression analysis,and qRT-PCR analysis of six perilla tissues sampled from two different phenotypes(serrated and smooth leaves)were performed.Results:Forty-three differentially expressed genes(DEGs),which may potentially regulate leaf shape,were identified through de novo transcriptome sequencing between the two groups.Genes involved in leaf shape regulation were identified.Simultaneously,we validated five DEGs by qRT-PCR,and the results were consistent with the transcriptome data.In addition,1186 transcription factors(TFs)belonging to 45 TF families were identified.Moreover,the co-expression network of DEGs was constructed.Conclusion:The study identified the key genes that control leaf shape by comparing the transcriptomes.Our findings also provide basic data for further exploring P.frutescens,which can help study the mechanism of leaf shape development and molecular breeding.
