Objective To explore the role and mechanism of palmitoyltransferase DHHC7 in colorectal cancer invasiveness.Methods Colorectacl ancer cell lines HCT116 with ZDHHC7 knocked out were the knockout group,and wild-type HCT...Objective To explore the role and mechanism of palmitoyltransferase DHHC7 in colorectal cancer invasiveness.Methods Colorectacl ancer cell lines HCT116 with ZDHHC7 knocked out were the knockout group,and wild-type HCT116 cells were the control group.The mRNA expression of the two groupsswas detected by transcriptome sequencing.The differentially expressed genes were screened,and the functions and signaling pathways which differentially expressed genes were involved in were preliminarily analyzed through Gene Ontology(GO)function analysis and Kyoto Encyclopedia of Genes and Genomes(KECG)pathway enrichment analysis,and effect of DHHC7 on the invasive capacity of tumor cells was verified by Transwell invasion assay.Results There were 2099 differentially expressed genes between the knockout group and the control group,of which 1190 were downregulated and 909 were upregulated.Significantly enriched GO items included cell adhesion,cell membrane composition,etc.The results of KECC pathway enrichment analysis suggested that DHHC7 might be involved in extracelluar matrix-receptor interaction and cell adhesion molecular pathway.The result of transwell assay confirmed that ZDHHC7 knockout could inhibit the invasion of tumor cells.Conclusion DHHC7 may promote the invasiveness of colorectal cancer by regulating the destruction of extracellular matrix and the expression of cell adhesion molecules.展开更多
文摘Objective To explore the role and mechanism of palmitoyltransferase DHHC7 in colorectal cancer invasiveness.Methods Colorectacl ancer cell lines HCT116 with ZDHHC7 knocked out were the knockout group,and wild-type HCT116 cells were the control group.The mRNA expression of the two groupsswas detected by transcriptome sequencing.The differentially expressed genes were screened,and the functions and signaling pathways which differentially expressed genes were involved in were preliminarily analyzed through Gene Ontology(GO)function analysis and Kyoto Encyclopedia of Genes and Genomes(KECG)pathway enrichment analysis,and effect of DHHC7 on the invasive capacity of tumor cells was verified by Transwell invasion assay.Results There were 2099 differentially expressed genes between the knockout group and the control group,of which 1190 were downregulated and 909 were upregulated.Significantly enriched GO items included cell adhesion,cell membrane composition,etc.The results of KECC pathway enrichment analysis suggested that DHHC7 might be involved in extracelluar matrix-receptor interaction and cell adhesion molecular pathway.The result of transwell assay confirmed that ZDHHC7 knockout could inhibit the invasion of tumor cells.Conclusion DHHC7 may promote the invasiveness of colorectal cancer by regulating the destruction of extracellular matrix and the expression of cell adhesion molecules.
