Objective:Recent cholera outbreaks in Anhui Province have been linked to Vibrio cholerae O139,but information on these strains is limited.We established the first genomic dataset of local O139 strains to analyze the g...Objective:Recent cholera outbreaks in Anhui Province have been linked to Vibrio cholerae O139,but information on these strains is limited.We established the first genomic dataset of local O139 strains to analyze the genomic characteristics and evolution of antibiotic resistance.Methods:Thirty-four Vibrio cholerae O139 isolates from Anhui(2013–2024)were sequenced using nextgeneration sequencing.Genes for virulence,antimicrobial resistance,pathogenicity islands,and mobile genetic elements were predicted using ABRicate and other online tools.To construct a phylogenetic tree,124 publicly available O139 genomes were included in the single-nucleotide polymorphism analysis alongside the study isolates.Results:Strains formed two clusters that were genetically closer to China isolates than with those from Bangladesh and India.All strains harbored ctxA and ctxB,with partial deletions in virulence genes and pathogenicity islands;over 50%lacked vgrG-2 in the T6SS.Strains from 2022–2024 exhibited higher azithromycin but lower trimethoprimsulfamethoxazole resistance than those collected during 2013–2017.Conclusion:Vibrio cholerae O139 in Anhui are endemic to China,with limited virulence but strong colonization abilities.The increased azithromycin resistance rate may be driven by its clinical antimicrobial usage,suggesting its potential for continued antibiotic resistance evolution.展开更多
基金Supported by the National Disease Control and Prevention Administration Public Health Talent Training Program(202303)the Open Fund of the State Key Laboratory of Tea Plant Biology and Utilization(SKLTOF20200131)+1 种基金the Scientific Research Projects of Health Commission of Anhui Province in 2022(AHWJ2022a022)the Scientific Research Projects of Health Commission of Anhui Province in 2023(AHWJ2023A20398).
文摘Objective:Recent cholera outbreaks in Anhui Province have been linked to Vibrio cholerae O139,but information on these strains is limited.We established the first genomic dataset of local O139 strains to analyze the genomic characteristics and evolution of antibiotic resistance.Methods:Thirty-four Vibrio cholerae O139 isolates from Anhui(2013–2024)were sequenced using nextgeneration sequencing.Genes for virulence,antimicrobial resistance,pathogenicity islands,and mobile genetic elements were predicted using ABRicate and other online tools.To construct a phylogenetic tree,124 publicly available O139 genomes were included in the single-nucleotide polymorphism analysis alongside the study isolates.Results:Strains formed two clusters that were genetically closer to China isolates than with those from Bangladesh and India.All strains harbored ctxA and ctxB,with partial deletions in virulence genes and pathogenicity islands;over 50%lacked vgrG-2 in the T6SS.Strains from 2022–2024 exhibited higher azithromycin but lower trimethoprimsulfamethoxazole resistance than those collected during 2013–2017.Conclusion:Vibrio cholerae O139 in Anhui are endemic to China,with limited virulence but strong colonization abilities.The increased azithromycin resistance rate may be driven by its clinical antimicrobial usage,suggesting its potential for continued antibiotic resistance evolution.
