The Fujian oyster(Crassostrea angulata) is an economically significant shellfish species distributed mainly along the Fujian coast, Southeast China. However, its genetic diversity and structure remain unclear. The mai...The Fujian oyster(Crassostrea angulata) is an economically significant shellfish species distributed mainly along the Fujian coast, Southeast China. However, its genetic diversity and structure remain unclear. The main distribution area of the C. angulata is located in Fujian, South China. In total, 420 C. angulata were collected from 14 natural habitats(populations) along the Fujian coast, and their genetic diversity and structure were analyzed in the mitochondrial COI and nuclear gene ITS2 sequences. Results reveal that all the 14 populations of C. angulata exhibited high levels of genetic diversity, with a total of 57(haplotype diversity: 0.811±0.016) and 124(haplotype diversity: 0.912±0.007) haplotypes revealed by COI and ITS2, respectively. Notably, significant intermediate level of genetic differentiations between the Ningde Zhujiang(ZJ) population(FS T by COI: 0.035–0.142, P<0.05;FS T by ITS2: 0.078–0.123, P<0.05) with other populations were observed for the first time, which is also supported by the results of molecular variance analysis(FC T by COI: 0.105, P<0.05;FC T by ITS2: 0.086, P<0.05) and the clustering of the ZJ population into distinct branches in the interpopulation genetic differentiation tree. Furthermore, the evolutionary tree and haplotype network analyses do not support the formation of a clear geographical genealogical structure among these 14 populations. In addition, the population dynamics analysis suggests that the C. angulata may have undergone expansion during the third ice age of the Pleistocene. These results provide a reference for the preservation and further genetic improvement of C. angulata.展开更多
Binary sequences constructed by Legendre symbols are widely used in communication and cryptography since they have many good pseudo-random properties.In this paper,we determine the 2-adic complexity of the sum sequenc...Binary sequences constructed by Legendre symbols are widely used in communication and cryptography since they have many good pseudo-random properties.In this paper,we determine the 2-adic complexity of the sum sequence of any k many Legendre sequences and show that the 2-adic complexity of the sum sequences of any k many Legendre sequences reaches the maximum by proving the case of k=2 and 3,which implies that the sum sequences can resist the attack of rational approximation algorithm.展开更多
In this paper,we introduce non-abelian cohomology groups and classify the nonabelian extensions of Rota-Baxter pre-Lie algebras in terms of non-abelian cohomology groups.Next,we explore the inducibility of pairs of au...In this paper,we introduce non-abelian cohomology groups and classify the nonabelian extensions of Rota-Baxter pre-Lie algebras in terms of non-abelian cohomology groups.Next,we explore the inducibility of pairs of automorphisms and derive the analog Wells exact sequences under the circumstance of Rota-Baxter pre-Lie algebras.Finally,we discuss the inducibility problem of pairs of automorphisms about an abelian extensions of Rota-Baxter pre-Lie algebras.展开更多
目的研究大连市2023—2024年分离的18株布鲁氏菌基因组特征,为布鲁氏菌分子溯源、毒力基因分析以及耐药机制的研究提供数据支持。方法对大连市2023—2024年分离自患者的18株布鲁氏菌进行生化鉴定和全基因组测序,利用基因组分析软件对全...目的研究大连市2023—2024年分离的18株布鲁氏菌基因组特征,为布鲁氏菌分子溯源、毒力基因分析以及耐药机制的研究提供数据支持。方法对大连市2023—2024年分离自患者的18株布鲁氏菌进行生化鉴定和全基因组测序,利用基因组分析软件对全基因组数据进行质控和基因组组装,同时从NCBI下载布鲁氏菌基因组序列,对组装基因组开展多位点序列分型(MLST)、全基因组单核苷酸多态性分析(whole-genome Single Nucleotide Polymorphism,wgSNP)、毒力基因和耐药基因分析。结果MLST分型结果:18株布鲁氏菌均为羊种布鲁氏菌ST8型。wgSNP结果:18株本地菌株和其他22株菌株被分为40个SNP型,所有菌株间均存在SNP差异,总SNP数为20590。通过系统发育分析,40株菌被分为3个主进化分支,2株本地菌株与呼和浩特、河北分离的菌株亲缘关系较近,位于主进化分支1上;4株本地菌株与通辽分离的菌株亲缘关系较近,位于主进化分支2上;其余12株本地菌株与其他19株菌株亲缘关系较近,均位于主进化分支3上,12株本地菌株与2株北京分离株、2株呼伦贝尔分离株位于主进化分支3的亚分支3b上,其他种的布鲁氏菌参考株均位于主进化分支3的亚分支3a上。DL230017BRU与DL231021BRU、DL240794BRU与DL240723BRU之间的SNP数均为2。18株菌株均有LPS、T4SS等13种毒力基因和adeF、Brucella suis mprF两种耐药基因。结论没有足够的流行病学信息支持这18株菌株为同一来源,且部分菌株与国内其他地区分离的菌株亲缘关系较近,但这些菌株具有潜在的致病性和耐药性,有必要开展连续监测,为布鲁氏菌病防控提供依据。展开更多
目的探讨动脉粥样硬化(AS)患者口腔菌群多样性与炎症因子水平的特征及关联。方法纳入AS患者50例与健康对照50例,采集唾液和血样进行16S rRNA测序、IL-6、IL-1β、TNF-α、CRP检测;比较两组菌群结构与炎症指标差异,并进行Spearman相关和...目的探讨动脉粥样硬化(AS)患者口腔菌群多样性与炎症因子水平的特征及关联。方法纳入AS患者50例与健康对照50例,采集唾液和血样进行16S rRNA测序、IL-6、IL-1β、TNF-α、CRP检测;比较两组菌群结构与炎症指标差异,并进行Spearman相关和多因素回归分析。结果AS组IL-6、IL-1β、TNF-α、CRP水平均显著高于对照组(IL-6:8.24±2.15 vs 6.15±1.76,P<0.01;CRP:7.42±2.41 vs 3.98±1.57,P<0.01);口腔菌群Shannon指数低于对照组(4.38±0.55 vs 4.61±0.52,P=0.040);Fusobacterium与CRP、IL-6正相关(r=0.41、0.36,均P<0.05)。多因素回归提示Fusobacterium丰度及IL-6、CRP水平均为AS潜在危险因素。结论AS患者口腔菌群多样性下降伴随炎症显著增高,二者或通过免疫和代谢途径相互作用,为AS的风险评估与干预策略提供新的思路。展开更多
[目的]雌牦牛性成熟晚是影响牦牛繁殖率的重要原因之一,分析不同发育时期牦牛卵巢的全基因组DNA甲基化差异,筛选出调控卵巢卵泡发育的差异甲基化基因,为研究表观遗传在牦牛卵巢组织生长发育过程中的作用提供数据基础。[方法]分别选取0.5...[目的]雌牦牛性成熟晚是影响牦牛繁殖率的重要原因之一,分析不同发育时期牦牛卵巢的全基因组DNA甲基化差异,筛选出调控卵巢卵泡发育的差异甲基化基因,为研究表观遗传在牦牛卵巢组织生长发育过程中的作用提供数据基础。[方法]分别选取0.5岁(幼年)、2.5岁(初情期)和4.5岁(性成熟)的牦牛各3头,采用全基因组重亚硫酸盐测序法,检测分析不同发育时期牦牛卵巢组织的全基因组DNA甲基化谱。[结果]牦牛卵巢组织中,甲基化位点以CpG序列类型为主,其甲基化水平明显高于CHG和CHH类型。CpG序列在CDS区、内含子和3′UTR的甲基化水平高于基因下游2 kb、基因上游2 kb和5′UTR。对比3个年龄组的甲基化位点,统计出3种类型的差异甲基化区域数量分别为4233(0.5 vs 2.5)、7302(2.5 vs 4.5)和1133(0.5 vs 4.5),其中CpG序列的差异甲基化区域分别对应334(0.5 vs 2.5)、640(2.5 vs 4.5)和371(0.5 vs 4.5)个差异甲基化基因。采用重亚硫酸盐测序法对测序结果进一步验证,结果显示变化趋势一致。GO和KEGG富集分析结果显示,CpG序列的差异甲基化基因显著富集到包括卵母细胞减数分裂、剪接体通路、Hippo信号通路、催产素信号通路、GnRH信号通路、Wnt信号通路、钙信号通路和Rap1信号通路等与卵巢卵泡发育相关的通路(P<0.05)。并从这些通路中筛选出8个甲基化差异显著且与牦牛卵巢卵泡发育相关的基因作为候选基因(P<0.05)。[结论]本研究利用全基因组重亚硫酸盐测序法检测分析了不同发育阶段牦牛卵巢全基因组甲基化谱,筛选出调控卵巢卵泡发育的8个候选基因,为进一步研究DNA甲基化对牦牛卵巢生长发育的调控机制提供数据基础。展开更多
The first internal transcribed spacer(ITS1) of nuclear ribosomal DNA of three wild rice species and two subspecies of cultivated rice, which are distributed in China, was amplified using PCR technique and sequenced wi...The first internal transcribed spacer(ITS1) of nuclear ribosomal DNA of three wild rice species and two subspecies of cultivated rice, which are distributed in China, was amplified using PCR technique and sequenced with automated fluorescent sequencing. The sequences of ITS1 ranged from 193 bp to 218 bp in size and G/C content varied from 69.3% to 72.7%. In pairwise comparisons among the five taxa, sequence site divergence ranged from 1.5% to 10.6%. Phylogenetic analysis of ITS1 sequences using Wagner parsimony generated a single well resolved tree, which revealed that Oryza rufipogon was much more closely related to cultivated rice species than to the other two wild species. Oryza granulata was less closely related to either cultivated rice species or the other two wild species, and might be a unique and isolated taxon in the genus Oryza. The phylogenetic relationships of the three wild rice species and two cultivated rice subspecies inferred from ITS1 sequences is highly concordant with those based on the molecular evidence from isozyme, chloroplast DNA (cpDNA), mitochondrial DNA(mtDNA) and nuclear DNA (nDNA) of the genus Oryza .展开更多
基金Supported by the National Natural Science Foundation of China(No.32172979)the Natural Science Foundation of Fujian Province(No.2021J05159)the 2023 Special Program for Promoting High-Quality Development of Marine and Fishery Industry in Fujian Province(No.PJHYF-L-2023-2)。
文摘The Fujian oyster(Crassostrea angulata) is an economically significant shellfish species distributed mainly along the Fujian coast, Southeast China. However, its genetic diversity and structure remain unclear. The main distribution area of the C. angulata is located in Fujian, South China. In total, 420 C. angulata were collected from 14 natural habitats(populations) along the Fujian coast, and their genetic diversity and structure were analyzed in the mitochondrial COI and nuclear gene ITS2 sequences. Results reveal that all the 14 populations of C. angulata exhibited high levels of genetic diversity, with a total of 57(haplotype diversity: 0.811±0.016) and 124(haplotype diversity: 0.912±0.007) haplotypes revealed by COI and ITS2, respectively. Notably, significant intermediate level of genetic differentiations between the Ningde Zhujiang(ZJ) population(FS T by COI: 0.035–0.142, P<0.05;FS T by ITS2: 0.078–0.123, P<0.05) with other populations were observed for the first time, which is also supported by the results of molecular variance analysis(FC T by COI: 0.105, P<0.05;FC T by ITS2: 0.086, P<0.05) and the clustering of the ZJ population into distinct branches in the interpopulation genetic differentiation tree. Furthermore, the evolutionary tree and haplotype network analyses do not support the formation of a clear geographical genealogical structure among these 14 populations. In addition, the population dynamics analysis suggests that the C. angulata may have undergone expansion during the third ice age of the Pleistocene. These results provide a reference for the preservation and further genetic improvement of C. angulata.
文摘Binary sequences constructed by Legendre symbols are widely used in communication and cryptography since they have many good pseudo-random properties.In this paper,we determine the 2-adic complexity of the sum sequence of any k many Legendre sequences and show that the 2-adic complexity of the sum sequences of any k many Legendre sequences reaches the maximum by proving the case of k=2 and 3,which implies that the sum sequences can resist the attack of rational approximation algorithm.
基金Supported by the National Natural Science Foundation of China(Grant No.12161013)the School-Level Student Research Project of Guizhou University of Finance and Economics(Grant No.2024ZXSY239).
文摘In this paper,we introduce non-abelian cohomology groups and classify the nonabelian extensions of Rota-Baxter pre-Lie algebras in terms of non-abelian cohomology groups.Next,we explore the inducibility of pairs of automorphisms and derive the analog Wells exact sequences under the circumstance of Rota-Baxter pre-Lie algebras.Finally,we discuss the inducibility problem of pairs of automorphisms about an abelian extensions of Rota-Baxter pre-Lie algebras.
文摘目的研究大连市2023—2024年分离的18株布鲁氏菌基因组特征,为布鲁氏菌分子溯源、毒力基因分析以及耐药机制的研究提供数据支持。方法对大连市2023—2024年分离自患者的18株布鲁氏菌进行生化鉴定和全基因组测序,利用基因组分析软件对全基因组数据进行质控和基因组组装,同时从NCBI下载布鲁氏菌基因组序列,对组装基因组开展多位点序列分型(MLST)、全基因组单核苷酸多态性分析(whole-genome Single Nucleotide Polymorphism,wgSNP)、毒力基因和耐药基因分析。结果MLST分型结果:18株布鲁氏菌均为羊种布鲁氏菌ST8型。wgSNP结果:18株本地菌株和其他22株菌株被分为40个SNP型,所有菌株间均存在SNP差异,总SNP数为20590。通过系统发育分析,40株菌被分为3个主进化分支,2株本地菌株与呼和浩特、河北分离的菌株亲缘关系较近,位于主进化分支1上;4株本地菌株与通辽分离的菌株亲缘关系较近,位于主进化分支2上;其余12株本地菌株与其他19株菌株亲缘关系较近,均位于主进化分支3上,12株本地菌株与2株北京分离株、2株呼伦贝尔分离株位于主进化分支3的亚分支3b上,其他种的布鲁氏菌参考株均位于主进化分支3的亚分支3a上。DL230017BRU与DL231021BRU、DL240794BRU与DL240723BRU之间的SNP数均为2。18株菌株均有LPS、T4SS等13种毒力基因和adeF、Brucella suis mprF两种耐药基因。结论没有足够的流行病学信息支持这18株菌株为同一来源,且部分菌株与国内其他地区分离的菌株亲缘关系较近,但这些菌株具有潜在的致病性和耐药性,有必要开展连续监测,为布鲁氏菌病防控提供依据。
文摘目的探讨动脉粥样硬化(AS)患者口腔菌群多样性与炎症因子水平的特征及关联。方法纳入AS患者50例与健康对照50例,采集唾液和血样进行16S rRNA测序、IL-6、IL-1β、TNF-α、CRP检测;比较两组菌群结构与炎症指标差异,并进行Spearman相关和多因素回归分析。结果AS组IL-6、IL-1β、TNF-α、CRP水平均显著高于对照组(IL-6:8.24±2.15 vs 6.15±1.76,P<0.01;CRP:7.42±2.41 vs 3.98±1.57,P<0.01);口腔菌群Shannon指数低于对照组(4.38±0.55 vs 4.61±0.52,P=0.040);Fusobacterium与CRP、IL-6正相关(r=0.41、0.36,均P<0.05)。多因素回归提示Fusobacterium丰度及IL-6、CRP水平均为AS潜在危险因素。结论AS患者口腔菌群多样性下降伴随炎症显著增高,二者或通过免疫和代谢途径相互作用,为AS的风险评估与干预策略提供新的思路。
文摘[目的]雌牦牛性成熟晚是影响牦牛繁殖率的重要原因之一,分析不同发育时期牦牛卵巢的全基因组DNA甲基化差异,筛选出调控卵巢卵泡发育的差异甲基化基因,为研究表观遗传在牦牛卵巢组织生长发育过程中的作用提供数据基础。[方法]分别选取0.5岁(幼年)、2.5岁(初情期)和4.5岁(性成熟)的牦牛各3头,采用全基因组重亚硫酸盐测序法,检测分析不同发育时期牦牛卵巢组织的全基因组DNA甲基化谱。[结果]牦牛卵巢组织中,甲基化位点以CpG序列类型为主,其甲基化水平明显高于CHG和CHH类型。CpG序列在CDS区、内含子和3′UTR的甲基化水平高于基因下游2 kb、基因上游2 kb和5′UTR。对比3个年龄组的甲基化位点,统计出3种类型的差异甲基化区域数量分别为4233(0.5 vs 2.5)、7302(2.5 vs 4.5)和1133(0.5 vs 4.5),其中CpG序列的差异甲基化区域分别对应334(0.5 vs 2.5)、640(2.5 vs 4.5)和371(0.5 vs 4.5)个差异甲基化基因。采用重亚硫酸盐测序法对测序结果进一步验证,结果显示变化趋势一致。GO和KEGG富集分析结果显示,CpG序列的差异甲基化基因显著富集到包括卵母细胞减数分裂、剪接体通路、Hippo信号通路、催产素信号通路、GnRH信号通路、Wnt信号通路、钙信号通路和Rap1信号通路等与卵巢卵泡发育相关的通路(P<0.05)。并从这些通路中筛选出8个甲基化差异显著且与牦牛卵巢卵泡发育相关的基因作为候选基因(P<0.05)。[结论]本研究利用全基因组重亚硫酸盐测序法检测分析了不同发育阶段牦牛卵巢全基因组甲基化谱,筛选出调控卵巢卵泡发育的8个候选基因,为进一步研究DNA甲基化对牦牛卵巢生长发育的调控机制提供数据基础。
基金The project supported by the Grant from Presidentof Chinese Academy of Sciences
文摘The first internal transcribed spacer(ITS1) of nuclear ribosomal DNA of three wild rice species and two subspecies of cultivated rice, which are distributed in China, was amplified using PCR technique and sequenced with automated fluorescent sequencing. The sequences of ITS1 ranged from 193 bp to 218 bp in size and G/C content varied from 69.3% to 72.7%. In pairwise comparisons among the five taxa, sequence site divergence ranged from 1.5% to 10.6%. Phylogenetic analysis of ITS1 sequences using Wagner parsimony generated a single well resolved tree, which revealed that Oryza rufipogon was much more closely related to cultivated rice species than to the other two wild species. Oryza granulata was less closely related to either cultivated rice species or the other two wild species, and might be a unique and isolated taxon in the genus Oryza. The phylogenetic relationships of the three wild rice species and two cultivated rice subspecies inferred from ITS1 sequences is highly concordant with those based on the molecular evidence from isozyme, chloroplast DNA (cpDNA), mitochondrial DNA(mtDNA) and nuclear DNA (nDNA) of the genus Oryza .
