Sequence-activated chemiluminescence(CL)technique offers a unique approach to detecting specific biomolecules or cellular events with high sensitivity and spatial resolution.The fundamental principle behind sequence-a...Sequence-activated chemiluminescence(CL)technique offers a unique approach to detecting specific biomolecules or cellular events with high sensitivity and spatial resolution.The fundamental principle behind sequence-activated CL probes involves tailoring highly-controllable CL signals in response to specific sequences or events of interest,thus enabling high-contrast signals under complex physiological conditions.This sequential activation could be triggered by enzymes,reactive oxygen species(ROS),biothiols or changes in the microenvironment.These unique sequence-activated CL probes bestow several advantages,including minimal background,deep-tissue penetration,and the ability of non-invasive or minimally invasive detection.The sequence-activated CL techniques represent a significant advancement in molecular imaging and diagnostics.In this mini-review,we focus on the latest developments in sequential CL probes,addressing the technical challenges and discussing the future trajectory by harnessing CL signaling and molecular recognition.展开更多
基金supported by the National Key Research and Development Program of China(2023YFA1802000,2021YFA0910000)the National Natural Science Foundation of China(22225805,22308101,32394001,32121005)+1 种基金the Shanghai Science and Technology Innovation Action Plan(23J21901600)the Innovation Program of Shanghai Municipal Education Commission,China Postdoctoral Science Foundation(2021M701199)。
文摘Sequence-activated chemiluminescence(CL)technique offers a unique approach to detecting specific biomolecules or cellular events with high sensitivity and spatial resolution.The fundamental principle behind sequence-activated CL probes involves tailoring highly-controllable CL signals in response to specific sequences or events of interest,thus enabling high-contrast signals under complex physiological conditions.This sequential activation could be triggered by enzymes,reactive oxygen species(ROS),biothiols or changes in the microenvironment.These unique sequence-activated CL probes bestow several advantages,including minimal background,deep-tissue penetration,and the ability of non-invasive or minimally invasive detection.The sequence-activated CL techniques represent a significant advancement in molecular imaging and diagnostics.In this mini-review,we focus on the latest developments in sequential CL probes,addressing the technical challenges and discussing the future trajectory by harnessing CL signaling and molecular recognition.
