It is critical to acknowledge that the coexistence of genes related to carbapenem and polymyxin resistance can exacerbate challenges in antimicrobial therapy.In this study,we identified Klebsiella pneumoniae isolate L...It is critical to acknowledge that the coexistence of genes related to carbapenem and polymyxin resistance can exacerbate challenges in antimicrobial therapy.In this study,we identified Klebsiella pneumoniae isolate L2425 fromthe feces of an ICU patient in China.The isolate belongs to sequence type 11 and carries the antimicrobial resistance genes blaKPC-2,bla_(CTX-M-65) and mcr-8.Through S1-nuclease pulsed-field gel electrophoresis and Southern blotting analysis,we confirmed the presence of a~110 kb plasmid harboring the mcr-8 gene with a conserved structure(inhA-mcr-8-ompR-dgkA-IS903B).Furthermore,another~130 kb plasmid carrying bla_(KPC-2) was detected along with a composite transposon flanked by insertion sequence IS26.Conjugation experiments demonstrated that both pL2425-KPC-2 and pL2425-MCR-8 plasmids were transferable.Importantly,this is the first instance of clinical fecal samples from China containing a K.pneumoniae strain carrying both blaKPC-2 and mcr-8 genes.These findings underscore the significance of surveillance for carbapenemases and mobile colistin–resistance variants.展开更多
基金supported by the National Key Research and Development Programof China(2019YFC0840600,2019YFC0840609 and 2020YFE0204300)the National Natural Science Foundation of China(82072314)+3 种基金the Zhejiang Province Natural Science Foundation of China(LHDMZ22H190002 and LY19H160060)the Shandong Provincial Laboratory Project(SYS202202)the Research Project of Jinan Microecological Biomedicine Shandong Laboratory(JNL-2022011B),the Fundamental Research Funds for the Central Universities(2022ZFJH003)the CAMS Innovation Fund for Medical Sciences(2019-I2M-5-045).
文摘It is critical to acknowledge that the coexistence of genes related to carbapenem and polymyxin resistance can exacerbate challenges in antimicrobial therapy.In this study,we identified Klebsiella pneumoniae isolate L2425 fromthe feces of an ICU patient in China.The isolate belongs to sequence type 11 and carries the antimicrobial resistance genes blaKPC-2,bla_(CTX-M-65) and mcr-8.Through S1-nuclease pulsed-field gel electrophoresis and Southern blotting analysis,we confirmed the presence of a~110 kb plasmid harboring the mcr-8 gene with a conserved structure(inhA-mcr-8-ompR-dgkA-IS903B).Furthermore,another~130 kb plasmid carrying bla_(KPC-2) was detected along with a composite transposon flanked by insertion sequence IS26.Conjugation experiments demonstrated that both pL2425-KPC-2 and pL2425-MCR-8 plasmids were transferable.Importantly,this is the first instance of clinical fecal samples from China containing a K.pneumoniae strain carrying both blaKPC-2 and mcr-8 genes.These findings underscore the significance of surveillance for carbapenemases and mobile colistin–resistance variants.
