Based on stochastic optimization strategy, a formulation methodology is proposed for synthesizing distillation column sequences, allowing more than one middle component as the distributing components between a pair of...Based on stochastic optimization strategy, a formulation methodology is proposed for synthesizing distillation column sequences, allowing more than one middle component as the distributing components between a pair of key components in the non-sharp split. In order to represent and manipulate the distillation configuration structures, a new coding procedure is proposed in the form of one-dimensional array. Theoretically, an array can represent any kind of split(non-sharp and sharp). With the application of a binary sort tree approach, a robust flow sheet encoding and decoding procedure is developed so that the problem formulation and solution becomes tractable. In this paper,the synthesis problem is formulated as a mixed-integer nonlinear programming(MINLP) problem and an improved simulated annealing approach is adopted to solve the optimization problem. Besides, a shortcut method is applied to the evaluation of all required design parameters as well as the total function.展开更多
Microbiome-wide association studies(MWASs)have uncovered microbial markers linked to ecosystem traits,but the mechanisms underlying their functions can remain elusive.This is largely due to challenges in validating th...Microbiome-wide association studies(MWASs)have uncovered microbial markers linked to ecosystem traits,but the mechanisms underlying their functions can remain elusive.This is largely due to challenges in validating their in situ metabolic activities and tracing such activities to individual genomes.Here,we introduced a phylogeny-metabolism dual-directed singlecell genomics approach called fluorescence-in situ-hybridization-guided single-cell Raman-activated sorting and sequencing(FISH-scRACS-seq).It directly localizes individual cells from target taxon via an FISH probe for marker organism,profiles their in situ metabolic functions via single-cell Raman spectra,sorts cells of target taxonomy and target metabolism,and produces indexed,high-coverage,and precisely-one-cell genomes.From cyclohexane-contaminated seawater,cells representing the MWAS-derived marker taxon of g-Proteobacteria and that are actively degrading cyclohexane in situ were directly identified via FISH and Raman,respectively,then sorted and sequenced for one-cell full genomes.In such a Pseudoalteromonas fuliginea cell,we discovered a three-component cytochrome P450 system that can convert cyclohexane to cyclohexanol in vitro,representing a previously unknown group of cyclohexane-degrading enzymes and organisms.Therefore,by unveiling enzymes,pathways,genomes,and their in situ cellular functions specifically for those organisms with ecological relevance at one-cell resolution,FISH-scRACS-seq is a rational and generally applicable approach to dissecting and mining microbiota functions.展开更多
基金Supported by the 973 Program of China(2012CB720503)the Open Research Project of State Kay Laboratory of Chemical Engineering(SKL-Ch E-13B02)
文摘Based on stochastic optimization strategy, a formulation methodology is proposed for synthesizing distillation column sequences, allowing more than one middle component as the distributing components between a pair of key components in the non-sharp split. In order to represent and manipulate the distillation configuration structures, a new coding procedure is proposed in the form of one-dimensional array. Theoretically, an array can represent any kind of split(non-sharp and sharp). With the application of a binary sort tree approach, a robust flow sheet encoding and decoding procedure is developed so that the problem formulation and solution becomes tractable. In this paper,the synthesis problem is formulated as a mixed-integer nonlinear programming(MINLP) problem and an improved simulated annealing approach is adopted to solve the optimization problem. Besides, a shortcut method is applied to the evaluation of all required design parameters as well as the total function.
基金supported by grants from the National Key R&D Program Young Scientists Project of China(2021YFD1900400)the Natural Science Foundation of China(32030003,32270109,32370097,42076165,and 32071266).
文摘Microbiome-wide association studies(MWASs)have uncovered microbial markers linked to ecosystem traits,but the mechanisms underlying their functions can remain elusive.This is largely due to challenges in validating their in situ metabolic activities and tracing such activities to individual genomes.Here,we introduced a phylogeny-metabolism dual-directed singlecell genomics approach called fluorescence-in situ-hybridization-guided single-cell Raman-activated sorting and sequencing(FISH-scRACS-seq).It directly localizes individual cells from target taxon via an FISH probe for marker organism,profiles their in situ metabolic functions via single-cell Raman spectra,sorts cells of target taxonomy and target metabolism,and produces indexed,high-coverage,and precisely-one-cell genomes.From cyclohexane-contaminated seawater,cells representing the MWAS-derived marker taxon of g-Proteobacteria and that are actively degrading cyclohexane in situ were directly identified via FISH and Raman,respectively,then sorted and sequenced for one-cell full genomes.In such a Pseudoalteromonas fuliginea cell,we discovered a three-component cytochrome P450 system that can convert cyclohexane to cyclohexanol in vitro,representing a previously unknown group of cyclohexane-degrading enzymes and organisms.Therefore,by unveiling enzymes,pathways,genomes,and their in situ cellular functions specifically for those organisms with ecological relevance at one-cell resolution,FISH-scRACS-seq is a rational and generally applicable approach to dissecting and mining microbiota functions.
