The elevation of Qinghai\|Tibetan plateau is a epoch\|making event in the global evolutionary history, which not only constructed the modern magnificent geomorphic feature, but also influenced far\|reachingly the glob...The elevation of Qinghai\|Tibetan plateau is a epoch\|making event in the global evolutionary history, which not only constructed the modern magnificent geomorphic feature, but also influenced far\|reachingly the global climate. The evolutionary models of its uplifting time and scope has been primarily set up, and is waiting to be competed with new discovery and supplement. A comprehensive field geologic excursion on the Cenozoic strata of the Kumukuli basin, Xinjiang, northwest Qinghai\|Tibetan plateau by authors has collected a lot of first\|hand data, and the discovery and dating of the late Pleistocene mammal fossils made an important supplement to the scarcity of late Quaternary fossil recorders in many basins, as well as contributed a great deal to the quantitative study of the researches alike, in the main time, provided new evidences to the uplift of Qinghai\|Tibetan plateau since late pleistocene.Lies in the northwest part of the Qinghai\|Tibetan plateau, and between the Kunlun and Altum Mountain ranges, Kumukuli basin is a intracontinental plateau basin, with a mean elevation about 4000m. Cenozoic strata have a time span from Oligocene to Pleistocene.The stratigraphic and lithologic association displays that the development of Kumukuli basin started at the Oligocene, at the primary uplift stage of the Qinghai\|Tibetan plateau. The Oligocene and Miocene, with a giant thickness and multiple layers of thick conglomerate, is of the products of stronger erosional stage, and a reflection of higher differential in inner geomorphology of the plateau. The aggradation led to the smoothness of the geomorphic feature and fineness of sedimentary particle since Pliocene. Since then, Large scale conglomerate outcroped in the peripheral of the plateau, and differential of geomorphology in the plateau and its peripheral areas became distinct. Kumukuli basin is one of the synchronously developed basin with the plateau, having the most completed Cenozoic sequence with a thickness over 7000m. Its Tertiary sedimentary sequence is basically similar to that of the Ningxia basin, northeastern margin of the Qinghai\|Tibetan plateau, and can be well correlated each other, mutually, they recorded the uplift process of the Qinghai\|Tibetan plateau, and their difference may represent the contrast evolution of the plateau and inhomogeneous differential in geomorphic feature of the plateau and its perimeter respectively. Being two sedimentary basins in separately tectonic units, the former has much thicker strata and coarser particles, for instance, the Oligocene is over 3000m in thickness in Kumukuli basin, however, only about 100m in Ningxia basin. Several uncomformities occurring in Kumukuli basin, namely, between the Quarternary and the Pliocene, the Pliocene and the Middle\|Upper Miocene, as well as the Middle\|Upper Miocene and the Lower Miocene, are the direct effects of horizontal movement accompanied with the process of the uplift of the Qinghai\|Tibetan plateau. Maybe those data imply that the western part of the plateau much stronger in uplift and distinct in horizontal movement than that of the east.展开更多
目的构建人白细胞介素 10重组腺病毒载体 ,为真核表达及其临床研究提供实验基础。方法自行设计一对引物 ,应用逆转录多聚酶链式反应 ( RT- PCR )技术 ,从活化的正常人外周血单个核细胞中扩增出 h IL - 10 c DNA,并将h IL - 10 c DNA克...目的构建人白细胞介素 10重组腺病毒载体 ,为真核表达及其临床研究提供实验基础。方法自行设计一对引物 ,应用逆转录多聚酶链式反应 ( RT- PCR )技术 ,从活化的正常人外周血单个核细胞中扩增出 h IL - 10 c DNA,并将h IL - 10 c DNA克隆到腺病毒载体 p Ad CMV - L ink1中 ,构建 p Ad CMV/ h IL - 10表达质粒。将此表达质粒与腺病毒重组质粒p JM17共转染 2 93细胞 ,通过同源重组产生 h IL - 10重组腺病毒。结果扩增到的 c DNA片段经酶切鉴定、PCR扩增、DNA测序最终确定为 h IL- 10 c DNA;所得人白细胞介素 10重组腺病毒滴度为 1.9× 10 9pfu/ m L。结论本实验为今后研究 h IL-展开更多
文摘The elevation of Qinghai\|Tibetan plateau is a epoch\|making event in the global evolutionary history, which not only constructed the modern magnificent geomorphic feature, but also influenced far\|reachingly the global climate. The evolutionary models of its uplifting time and scope has been primarily set up, and is waiting to be competed with new discovery and supplement. A comprehensive field geologic excursion on the Cenozoic strata of the Kumukuli basin, Xinjiang, northwest Qinghai\|Tibetan plateau by authors has collected a lot of first\|hand data, and the discovery and dating of the late Pleistocene mammal fossils made an important supplement to the scarcity of late Quaternary fossil recorders in many basins, as well as contributed a great deal to the quantitative study of the researches alike, in the main time, provided new evidences to the uplift of Qinghai\|Tibetan plateau since late pleistocene.Lies in the northwest part of the Qinghai\|Tibetan plateau, and between the Kunlun and Altum Mountain ranges, Kumukuli basin is a intracontinental plateau basin, with a mean elevation about 4000m. Cenozoic strata have a time span from Oligocene to Pleistocene.The stratigraphic and lithologic association displays that the development of Kumukuli basin started at the Oligocene, at the primary uplift stage of the Qinghai\|Tibetan plateau. The Oligocene and Miocene, with a giant thickness and multiple layers of thick conglomerate, is of the products of stronger erosional stage, and a reflection of higher differential in inner geomorphology of the plateau. The aggradation led to the smoothness of the geomorphic feature and fineness of sedimentary particle since Pliocene. Since then, Large scale conglomerate outcroped in the peripheral of the plateau, and differential of geomorphology in the plateau and its peripheral areas became distinct. Kumukuli basin is one of the synchronously developed basin with the plateau, having the most completed Cenozoic sequence with a thickness over 7000m. Its Tertiary sedimentary sequence is basically similar to that of the Ningxia basin, northeastern margin of the Qinghai\|Tibetan plateau, and can be well correlated each other, mutually, they recorded the uplift process of the Qinghai\|Tibetan plateau, and their difference may represent the contrast evolution of the plateau and inhomogeneous differential in geomorphic feature of the plateau and its perimeter respectively. Being two sedimentary basins in separately tectonic units, the former has much thicker strata and coarser particles, for instance, the Oligocene is over 3000m in thickness in Kumukuli basin, however, only about 100m in Ningxia basin. Several uncomformities occurring in Kumukuli basin, namely, between the Quarternary and the Pliocene, the Pliocene and the Middle\|Upper Miocene, as well as the Middle\|Upper Miocene and the Lower Miocene, are the direct effects of horizontal movement accompanied with the process of the uplift of the Qinghai\|Tibetan plateau. Maybe those data imply that the western part of the plateau much stronger in uplift and distinct in horizontal movement than that of the east.
文摘目的构建人白细胞介素 10重组腺病毒载体 ,为真核表达及其临床研究提供实验基础。方法自行设计一对引物 ,应用逆转录多聚酶链式反应 ( RT- PCR )技术 ,从活化的正常人外周血单个核细胞中扩增出 h IL - 10 c DNA,并将h IL - 10 c DNA克隆到腺病毒载体 p Ad CMV - L ink1中 ,构建 p Ad CMV/ h IL - 10表达质粒。将此表达质粒与腺病毒重组质粒p JM17共转染 2 93细胞 ,通过同源重组产生 h IL - 10重组腺病毒。结果扩增到的 c DNA片段经酶切鉴定、PCR扩增、DNA测序最终确定为 h IL- 10 c DNA;所得人白细胞介素 10重组腺病毒滴度为 1.9× 10 9pfu/ m L。结论本实验为今后研究 h IL-
