Temporomandibular joint(TMJ)disc displacement is one of the most significant subtypes of temporomandibular joint disorders,but its etiology and mechanism are poorly understood.In this study,we elucidated the mechanism...Temporomandibular joint(TMJ)disc displacement is one of the most significant subtypes of temporomandibular joint disorders,but its etiology and mechanism are poorly understood.In this study,we elucidated the mechanisms by which destruction of inflamed collagen fibrils induces alterations in the mechanical properties and positioning of the TMJ disc.By constructing a rat model of TMJ arthritis,we observed anteriorly dislocated TMJ discs with aggravated deformity in vivo from five weeks to six months after a local injection of Freund’s complete adjuvant.By mimicking inflammatory conditions with interleukin-1 beta in vitro,we observed enhanced expression of collagen-synthesis markers in primary TMJ disc cells cultured in a conventional two-dimensional environment.In contrast,three-dimensional(3D)-cultivated disc cell sheets demonstrated the disordered assembly of inflamed collagen fibrils,inappropriate arrangement,and decreased Young’s modulus.Mechanistically,inflammation-related activation of the nuclear factor kappa-B(NF-κB)pathway occurs during the progression of TMJ arthritis.NF-κB inhibition reduced the collagen fibril destruction in the inflamed disc cell sheets in vitro,and early NF-κB blockade alleviated collagen degeneration and dislocation of the TMJ discs in vivo.Therefore,the NF-κB pathway participates in the collagen remodeling in inflamed TMJ discs,offering a potential therapeutic target for disc displacement.展开更多
Catalytic destruction is an ascendant technology for the abatement of volatile organic compounds(VOCs)originating fromsolvent-based industrial processes.The varied composition tends to influence each VOC’s catalytic ...Catalytic destruction is an ascendant technology for the abatement of volatile organic compounds(VOCs)originating fromsolvent-based industrial processes.The varied composition tends to influence each VOC’s catalytic behavior in the reaction mixture.We investigated the catalytic destruction of multi-component VOCs including dichloromethane(DCM)and ethyl acetate(EA),as representatives from pharmaceutical waste gases,over co-supported HxPO_(4)-RuOx/CeO_(2) catalyst.A mutual inhibitory effect relating to concentrations because of competitive adsorption was verified in the binary VOCs oxidation and EA posed a more negative effect on DCM oxidation owing to EA’s superior adsorption capacity.Preferential adsorption of EA on acidic sites(HxPO_(4)/CeO_(2))promoted DCM activation on basic sites(O^(2−))and the dominating EA oxidation blocked DCM’s access to oxidation centers(RuOx/CeO_(2)),resulting in boosted monochloromethane yield and increased chlorine deposition for DCM oxidation.The impaired redox ability of Ru species owing to chlorine deposition in turn jeopardized deep oxidation of EA and its by-products,leading to increased gaseous by-products such as acetic acid originating fromEA pyrolysis.Notably,DCM at low concentration slightly promoted EA conversion at low temperatures with or without water,consistent with the enhanced EA adsorption in co-adsorption analyses.This was mainly due to that DCM impeded the shielding effect of hydrolysate deposition from rapid EA hydrolysis depending on the decreased acidity.Moreover,water benefited EA hydrolysis but decreased CO_(2) selectivity while the generated water derived from EA was likely to affect DCM transformation.This work may provide theoretical guidance for the promotion of applied catalysts toward industrial applications.展开更多
With the continuous advancement of communication and unmanned aerial vehicle(UAV)technologies,the collaborative operations of diverse platforms,including UAVs and ground vehicles,have been significantly promoted.Howev...With the continuous advancement of communication and unmanned aerial vehicle(UAV)technologies,the collaborative operations of diverse platforms,including UAVs and ground vehicles,have been significantly promoted.However,battlefield uncertainties,such as equipment failures and enemy attacks,can impact these collaborative operations'stability and communication efficiency.To this end,we design a highly destruction-resistant air-ground cooperative resilient networking platform that aims to enhance the robustness of network communications by integrating ground vehicle information for UAV network deployment.It then incorporates the concept of virtual guiding force,enabling the UAV swarm to adaptively configure its network layout based on ground vehicle information,thereby improving network destruction resistance.Simulation results demonstrate that the UAV swarm involved in the proposed platform exhibits balanced flight energy consumption and excellent performance in network destruction resistance.展开更多
Photocatalytic disinfection is an eco-friendly strategy for countering bacterial pollution in aquatic environments.Numerous strategies have been devised to facilitate the generation of reactive oxygen species(ROS)with...Photocatalytic disinfection is an eco-friendly strategy for countering bacterial pollution in aquatic environments.Numerous strategies have been devised to facilitate the generation of reactive oxygen species(ROS)within photocatalysts,ultimately leading to the eradication of bacteria.However,the significance of the physical morphology of photocatalysts in the context of sterilization is frequently obscured,and the progress in the development of physical-chemical synergistic sterilization photocatalysts has been relatively limited.Herein,graphitic carbon nitride(g-C_(3)N_(4))is chemically protonated to expose more sharp edges.PL fluorescence and EIS results indicate that the protonation can accelerate photogenerated carrier separation and enhance ROS production.Meanwhile,the sharp edges on the protonated g-C_(3)N_(4)facilitate the physical disruption of cell walls for further promoting oxidative damage.Protonated C_(3)N_(4)demonstrated superior bactericidal performance than that of pristine g-C_(3)N_(4),effectively eliminating Escherichia coli within 40 minutes under irradiation.This work highlights the significance of incorporating physical and chemical synergies in photocatalyst design to enhance the disinfection efficiency of photocatalysis.展开更多
BACKGROUND Although laparoscopic gastrolithotomy had been widely used in clinical practice,uncommon postoperative complications still require vigilance by medical staff.CASE SUMMARY Here we report a 67-year-old man wh...BACKGROUND Although laparoscopic gastrolithotomy had been widely used in clinical practice,uncommon postoperative complications still require vigilance by medical staff.CASE SUMMARY Here we report a 67-year-old man who suffered for 18 months and underwent surgery several times due to a rare and undetected complication of laparoscopic gastricolithotomy.He presented to multiple hospitals because of sustained left upper quadrant abdominal pain one month after laparoscopic gastricolithotomy due to a large gastric bezoar caused by unrestrained eating of black dates and was diagnosed with possible intercostal neuritis.Many painkillers were used to relieve his symptoms but the condition progressed.Seven months after surgery,he was hospitalized as skin ulceration occurred in the left upper abdominal wall and was subsequently diagnosed with a massive thoracoabdominal wall abscess.One year after surgery,irreversible costal destruction was demonstrated.Both lesions were finally proved to be secondary damage due to a rare chronic gastro-abdominal wall fistula related to laparoscopic gastricolithotomy and the diameter of the gastric fistula reached 2 centimeters(cm).The patient was ultimately cured but underwent multi-regional incisions and drainage of the abscess,drainage of the gastric fistula,partial gastrectomy and removal of damaged ribs,and was followed-up for more than 4 years without recurrence.It is well-known that gastric fistula usually has an acute onset and occurs early after surgery,while chronic gastro-abdominal wall fistula especially with secondary massive thoracoabdominal wall abscess and costal destruction has rarely been reported.CONCLUSION This may be the first reported case of a chronic thoracoabdominal abscess and costal destruction caused by an undetected chronic gastro-abdominal wall fistula.We believe that this is a novel type of gastric fistula and the diagnosis and treatment were challenging.展开更多
Dear Editor,I am Yuki Tanaka from Hakodate Central General Hospital,Japan.Mikulicz’s disease is characterized by symmetrical swelling of lacrimal and salivary glands.In 2012,a Japanese study group proposed comprehens...Dear Editor,I am Yuki Tanaka from Hakodate Central General Hospital,Japan.Mikulicz’s disease is characterized by symmetrical swelling of lacrimal and salivary glands.In 2012,a Japanese study group proposed comprehensive diagnostic criteria for immunoglobulin G4-related disease(IgG4-RD)^([1]).They have revealed that Mikulicz’s disease is a systemic IgG4-RD and attracted attentions of ophthalmologists.In 2014,the criteria for IgG4-related ophthalmic disease(IgG4-ROD)were established.展开更多
In order to improve the destruction efficiency of dioxins and also for developing new dioxin control technology, the destruction mechanisms of 2,3,7,8-tetrachlorodihenzo-p-dioxin （2,3,7,8-TCDD） by O3 and NO3, were i...In order to improve the destruction efficiency of dioxins and also for developing new dioxin control technology, the destruction mechanisms of 2,3,7,8-tetrachlorodihenzo-p-dioxin （2,3,7,8-TCDD） by O3 and NO3, were investigated employing quantum chemical calculations. For involved reactions, the microcosmic reaction processes were analyzed and depicted in detail based on geometry optimizations made by the B3LYP/6-31G（d） method. At the same time, the reaction activation energies were also calculated at the MP2/6- 311G（d,p）//B3LYP/6-31G（d） level. Configuration analysis indicated that 2,3,7,8-TCDD could be destroyed by 03 and NO3 in two different ways. The destruction of 2,3,7,8-TCDD by 03 proceeded via the addition of 03 and the cleavage of C=C while the destruction of 2,3,7,8-TCDD by NO3 proceeded via the substitution of chlorine by NO3. Calculated results show that, the activation energy of the destruction reaction of 2,3,7,8-TCDD by NO3 （267.48 kJ/mol） is much larger than that of the destruction reaction of 2,3,7,8-TCDD by O3 （51.20 kJ/mol）. This indicated that the destruction of 2,3,7,8-TCDD by 03 is much more efficient than that of 2,3,7,8-TCDD by NO3. The reason why the activation energy for the destruction reaction of 2,3,7,8-TCDD by NO3 is so large, is also discussed.展开更多
We aimed to investigate the synergistic effects of ultrasonic waves and repeated freeze-thaw cycles on cel-wal destruction of Auricularia auric-ula mycelia, and determine the best combination of conditions for cel-wal...We aimed to investigate the synergistic effects of ultrasonic waves and repeated freeze-thaw cycles on cel-wal destruction of Auricularia auric-ula mycelia, and determine the best combination of conditions for cel-wal destruc-tion of Auricularia auricula mycelia. [Method] The effects of destruction time, added water, destruction times, freeze time and number of freeze-thaw cycles during ultra-sonic treatment on polysaccharide yield were investigated by single-factor test in our research. The optimum conditions for cel-wal destruction of Auricularia auricula mycelia by the synergistic effect of ultrasonic waves and repeated freeze-thaw cycles were ascertained by orthogonal test. [Result] The results of single test indicated the best combination of conditions was as fol ows： freeze time, 30 min; destruction time, 20 min; added water, 15 times; destruction times, 2 times; number of freeze-thaw cycles, 3 cycles. The results of orthogonal test indicated the influencing factors ranked as destruction time 〉 destruction times 〉 freeze time. The best combination of conditions was as fol ows： freeze time, 30 min; destruction time, 25 min; destruc-tion times, 2 times. Under above conditions, the polysaccharide yield reached 57.76 mg/g. [Conclusion] This research would provide a basis and reference for practical production.展开更多
This essay is going to read the different kinds of beings in The Lord of the Rings as different stages of man,and to analyze how they construct or destruct their selves respectively over power.The Lord of the Rings is...This essay is going to read the different kinds of beings in The Lord of the Rings as different stages of man,and to analyze how they construct or destruct their selves respectively over power.The Lord of the Rings is read as a story about the self-construction and self-destruction of man,who begins to construct the sense of self from a very early stage in life,and becomes who he is in the continuous process of trying to identify himself with the image that keeps changing,of the frequently alternative construction and destruction of his self,and of intersubjectivity,the interrelation with other subjects.展开更多
PCDD/Fs have been become a serious issue because of their toxicological effects and associated adverse health implications. In this study, the gliding arc plasma was tested for treatment of polychlorinated dibenzo-p-d...PCDD/Fs have been become a serious issue because of their toxicological effects and associated adverse health implications. In this study, the gliding arc plasma was tested for treatment of polychlorinated dibenzo-p-dioxins （PCDDs） and polychlorinated dibenzofurans （PCDFs）, which was synthesized from pentachlorophenol in atmospheric condition at 350℃ with or without the catalysis of CuCl2. From the experiment, we found that the destruction efficiency of PCDD/F homologues after gliding was discharge ranged from 25% to 79%. This result demonstrates that gliding arc plasma is an effective technology to decompose PCDDs/Fs in flue gas. A plausible degradation mechanism for PCDD/Fs by gliding arc was discussed. Finally, a multistage reactor structure of gliding arc was proposed to upgrade removal efficiency for PCDD/Fs.展开更多
The destruction of methylphosphonic acid （MPA）, a final product by hydrolysis/neutralization of organophosphorus agents such as sarin and VX （O-ethyl S-[2-（diisopropylamino）ethyl] methylphosphonothionate）, was i...The destruction of methylphosphonic acid （MPA）, a final product by hydrolysis/neutralization of organophosphorus agents such as sarin and VX （O-ethyl S-[2-（diisopropylamino）ethyl] methylphosphonothionate）, was investigated in a a bench-scale, continuous concentric vertical double wall reactor under supercritical water oxidation condition. The experiments were conducted at a temperature range of 450–600°C and a fixed pressure of 25 MPa. Hydrogen peroxide was used as an oxidant. The destruction efficiency （DE） was monitored by analyzing total organic carbon （TOC） and MPA concentrations using ion chromatography on the liquid effluent samples. The results showed that the DE of MPA up to 99.999% was achieved at a reaction temperature of 600°C, oxygen concentration of 113% storichiometric requirement, and reactor residence time of 8 sec. On the basis of the data derived from experiments, a global kinetic rate equation for the DE of MPA and DE of TOC were developed by nonlinear regression analysis. The model predictions agreed well with the experimental data.展开更多
Destruction of organic contaminants in water by ozonation is a gas-liquid process which involves ozone mass transfer and fast irreversible chemical reactions. Ozonation reactor design and process optimizing require th...Destruction of organic contaminants in water by ozonation is a gas-liquid process which involves ozone mass transfer and fast irreversible chemical reactions. Ozonation reactor design and process optimizing require the modeling of the gas-liquid interactions within the reactor. In this paper a theoretical model combining the fluid dynamic and reaction kinetic parameters is proposed for predicting the destruction rates of organic pollutants in a semi-batch stirred-tank reactor by ozonation. A simple expression for the enhancement factor as our previous work has been applied to evaluate the chemical mass transfer coefficient in ozone absorption. 2,4-dichlorophenol (2,4-DCP) and 2,6-DCP or their mixture are chosen as the model compounds for simulating, and the predicted DCP concentrations are compared with some measured data.展开更多
[Objective]To investigate the inhibitory effect ofhonokiol on Candida albicans in vitro.[Methods]The minimum inhibitory concentration(MIC)and minimum fungicidal concentration(MFC)of magnolol for C.albicans were determ...[Objective]To investigate the inhibitory effect ofhonokiol on Candida albicans in vitro.[Methods]The minimum inhibitory concentration(MIC)and minimum fungicidal concentration(MFC)of magnolol for C.albicans were determined by microdilution method.Transmission electron microscopy was used to examine the effect of different concentrations of honokiol on the ultrastructure of C.albicans.Apoptosis of C.albicans caused by honokiol was analyzed by Annexin V-FITC/propidium iodide(PI)double staining and the accumulation of reactive oxygen species(ROS)in C.albicans cells was determined by DCFH-DA staining.The effects of honokiol on mitochondrial membrane potential and cell membrane permeability of C.albicans were analyzed by JC-1 staining and PI staining respectively.[Results]Honokiol had strong inhibitory effect on C.albicans,with MIC90 of 16μg/mL and MFC of 32μg/mL.Honokiol affected cell wall,cell membrane,and cytoplasm of C.albicans,induced both apoptosis and necrosis in C.albicans,probably through ROS production and disruption of mitochondrial function.It also changed the permeability of cell membrane,leaded to the cell wall damage and the binding of ergosterol.[Conclusion]Inhibition of C.albicans by honikiol involves multiple mechanisms including ROS production accompanied by a series of cellular damages.Honikiol appears to be a potential antifungal drug candidate.展开更多
In this study, we examined the effects of neuroglobin gene （Ngb） transfection into SH-SY5Y cells, using ultrasound-targeted microbubble destruction （UTMD）, on cobalt chloride-induced hypoxia. With an ultrasound in...In this study, we examined the effects of neuroglobin gene （Ngb） transfection into SH-SY5Y cells, using ultrasound-targeted microbubble destruction （UTMD）, on cobalt chloride-induced hypoxia. With an ultrasound intensity of 0.8 W/cm2, a 60-second exposure duration, 50% duty cycle, and 20% microbubble concentration, pAcGFP1-C1-Ngb-transfected cells exhibited the highest cell viability and transfection efficiency. The efficiency of plasmid delivery was significantly higher with UTMD than transfection with plasmid alone, transfection with plasmid using microbubbles, or transfection of plasmid by ultrasound. In addition, during cobalt chloride-induced hypoxia, caspase-3 activity in pAcGFP1-C1-Ngb-transfected cells was significantly lower than in untransfected cells. Ngb protein and mRNA expression were significantly higher in cells transfected by UTMD than in cells transfected with the other methods. These results demonstrate that UTMD can very efficiently mediate exogenous gene delivery, and that Ngb overexpression protects cells against cobalt chloride-induced hypoxia.展开更多
AIM： To detect the therapeutic effects of chemical destruction of celiac ganglion in patients with pancreatic carcinoma with intractable pain. METHODS： Ninety-seven cases with advanced pancreatic carcinoma received ...AIM： To detect the therapeutic effects of chemical destruction of celiac ganglion in patients with pancreatic carcinoma with intractable pain. METHODS： Ninety-seven cases with advanced pancreatic carcinoma received chemical destruction of celiac ganglion-5 mL pure alcohol injection around celiac artery under ultrasonic guidance. The changes of visual analogue scale （VAS）, serum substance P （Sub P）, β-endopeptide （β-EP） and T-lymphocyte subtypes level were compared between pre- and post-therapy. RESULTS： Successful rate of puncture was 98.7%, with one failure. No serious complications such as traumatic pancreatitis, pancreatic fistula, abdominal cavity hemorrhage or peritoneal infection occurred. VAS, serum Sub P and β-EP level significantly changed after treatment （8.0 ± 2.3 vs 4.6 ± 2.1, 254.1 ± 96.7 vs 182.4 ± 77.6, 3.2 ± 0.8 vs 8.8 ± 2.1, P 〈 0.01, P 〈 0.05, P 〈 0.01） with complete relief rate 54.2%, partial relief rate 21.9%, ineffective rate 12.5% and recurrent rate 10.7%. The T-lymphocyte subtypes level remarkably increased when compared with that of pre-therapy （46.7 ± 3.7 vs 62.5 ± 5.5, P 〈 0.01）. CONCLUSION： Our study suggests that chemical destruction of celiac ganglion under ultrasonic guidance is highly safe, and can evidently relieve cancer pain and improve the cellular immunity in patients with advanced pancreatic carcinoma.展开更多
The molecular pathogenesis of T-2 toxin-induced cartilage destruction has not been fully unraveled yet. The aim of this study was to detect changes in serum metabolites in a rat anomaly model with articular cartilage ...The molecular pathogenesis of T-2 toxin-induced cartilage destruction has not been fully unraveled yet. The aim of this study was to detect changes in serum metabolites in a rat anomaly model with articular cartilage destruction. Thirty healthy male Wistar rats were fed a diet containing T-2 toxin （300 ng/kg chow） for 3 months. Histopathological changes in femorotibial cartilage were characterized in terms of chondrocyte degeneration/necrosis and superficial cartilage defect, and the endogenous metabolite profile of serum was determined by UPLC/Q-TOF MS. Treated rats showed extensive areas of chondrocyte necrosis and superficial cartilage defect in the articular cartilage. In addition, 8 metabolites were found to change significantly in these rats compared to the control group, including lyso PE （18：0/0：0）, lyso PC（14：0）, lyso PC[18：4 （6Z,9Z,12Z,15Z）], lyso PC[（16：1（9Z）], lyso PC（16：0）, L-valine, hippuric acid, and asparaginyl-glycine. These 8 metabolites associated with cartilage injury are mainly involved in phospholipid and amino acid metabolic pathways.展开更多
The North China Craton(NCC)is the best example of an Archean craton that has lost its stability in the Late Mesozoic.Although the cratonic destruction is generally considered to have occurred in the Eastern Block and ...The North China Craton(NCC)is the best example of an Archean craton that has lost its stability in the Late Mesozoic.Although the cratonic destruction is generally considered to have occurred in the Eastern Block and reached a peak in the Early Cretaceous,the exact areal extent of cratonic destruction is debated,especially the southern and northern margin of the NCC.Here we report geochronology,geochemical and Hf isotopic data of the Late Mesozoic granitoids from Lushi polymetal mineralization area(LPMA)in the southern margin of NCC.These results provide new insights into the destruction in the southern margin of the NCC during the Late Mesozoic.Zircon U-Pb dating indicates that eight granitoids intruded in the Late Jurassic to Early Cretaceous(136.8–154.1 Ma),respectively.Geochemical signatures define these granitoids being A-type or I-type granite that formed in an extension setting.In addition,Hf isotopic compositions of zircons from these granitoids vary in a relatively large range,withεHf(t)values and TDM2 ages ranginge from-26.1 to+15.2 and 215 to 2849 Ma,respectively.The parental magmas were likely derived from diverse sources,including materials of the partial melting of ancient lower crust and mantle-derived mafic magmas in various proportions.Combining with previous studies on the contemporaneous magma-tectonic activities in circum of NCC,we suggest that the rim of NCC was already unstabilized from the Late Jurassic in the LPMA.The subduction of the Paleo-Pacific Plate was the main trigger to the destruction of the southern margin of NCC,which was responsible for the lithospheric extension and thinning,extensive magmatism and mineralization.展开更多
The immune-stromal cell interactions play a key role in health and diseases. In periodontitis, the most prevalent infectious disease in humans, immune cells accumulate in the oral mucosa and promote bone destruction b...The immune-stromal cell interactions play a key role in health and diseases. In periodontitis, the most prevalent infectious disease in humans, immune cells accumulate in the oral mucosa and promote bone destruction by inducing receptor activator of nuclear factor-κB ligand (RANKL) expression in osteogenic cells such as osteoblasts and periodontal ligament cells. However, the detailed mechanism underlying immune–bone cell interactions in periodontitis is not fully understood. Here, we performed single-cell RNAsequencing analysis on mouse periodontal lesions and showed that neutrophil–osteogenic cell crosstalk is involved in periodontitis-induced bone loss. The periodontal lesions displayed marked infiltration of neutrophils, and in silico analyses suggested that the neutrophils interacted with osteogenic cells through cytokine production. Among the cytokines expressed in the periodontal neutrophils, oncostatin M (OSM) potently induced RANKL expression in the primary osteoblasts, and deletion of the OSM receptor in osteogenic cells significantly ameliorated periodontitis-induced bone loss. Epigenomic data analyses identified the OSM-regulated RANKL enhancer region in osteogenic cells, and mice lacking this enhancer showed decreased periodontal bone loss while maintaining physiological bone metabolism. These findings shed light on the role of neutrophils in bone regulation during bacterial infection, highlighting the novel mechanism underlying osteoimmune crosstalk.展开更多
基金supported by the National Natural Science Foundation of China Nos.82370983,81671015(X.W.),82230030(Y.L.),82101043(S.C.)and 82370922(Y.F.)Beijing International Science and Technology Cooperation Project No.Z221100002722003(Y.L.)+4 种基金Beijing Natural Science Foundation Nos.L234017,JL23002(Y.L.),No.7242282(S.C.)and 7232217(Y.G.)Clinical Medicine Plus X-Young Scholars Project of Peking University No.PKU2024LCXQ039(Y.L.)National Program for Multidisciplinary Cooperative Treatment on Major Diseases No.PKUSSNMP-202013(X.W.)Hygiene and Health Development Scientific Research Fostering Plan of Haidian District Beijing No.HP2023-12-509001(J.Z.)Young Clinical Research Fund of the Chinese Stomatological Association No.CSA-02022-03(J.Z.).
文摘Temporomandibular joint(TMJ)disc displacement is one of the most significant subtypes of temporomandibular joint disorders,but its etiology and mechanism are poorly understood.In this study,we elucidated the mechanisms by which destruction of inflamed collagen fibrils induces alterations in the mechanical properties and positioning of the TMJ disc.By constructing a rat model of TMJ arthritis,we observed anteriorly dislocated TMJ discs with aggravated deformity in vivo from five weeks to six months after a local injection of Freund’s complete adjuvant.By mimicking inflammatory conditions with interleukin-1 beta in vitro,we observed enhanced expression of collagen-synthesis markers in primary TMJ disc cells cultured in a conventional two-dimensional environment.In contrast,three-dimensional(3D)-cultivated disc cell sheets demonstrated the disordered assembly of inflamed collagen fibrils,inappropriate arrangement,and decreased Young’s modulus.Mechanistically,inflammation-related activation of the nuclear factor kappa-B(NF-κB)pathway occurs during the progression of TMJ arthritis.NF-κB inhibition reduced the collagen fibril destruction in the inflamed disc cell sheets in vitro,and early NF-κB blockade alleviated collagen degeneration and dislocation of the TMJ discs in vivo.Therefore,the NF-κB pathway participates in the collagen remodeling in inflamed TMJ discs,offering a potential therapeutic target for disc displacement.
基金supported by the National Natural Science Foundation of China (Nos.21906087 and 52070168)the Key R&D Plan of Zhejiang Province (No.2023C03127)the Fundamental Research Funds for the Central Universities (No.226-2022-00150).
文摘Catalytic destruction is an ascendant technology for the abatement of volatile organic compounds(VOCs)originating fromsolvent-based industrial processes.The varied composition tends to influence each VOC’s catalytic behavior in the reaction mixture.We investigated the catalytic destruction of multi-component VOCs including dichloromethane(DCM)and ethyl acetate(EA),as representatives from pharmaceutical waste gases,over co-supported HxPO_(4)-RuOx/CeO_(2) catalyst.A mutual inhibitory effect relating to concentrations because of competitive adsorption was verified in the binary VOCs oxidation and EA posed a more negative effect on DCM oxidation owing to EA’s superior adsorption capacity.Preferential adsorption of EA on acidic sites(HxPO_(4)/CeO_(2))promoted DCM activation on basic sites(O^(2−))and the dominating EA oxidation blocked DCM’s access to oxidation centers(RuOx/CeO_(2)),resulting in boosted monochloromethane yield and increased chlorine deposition for DCM oxidation.The impaired redox ability of Ru species owing to chlorine deposition in turn jeopardized deep oxidation of EA and its by-products,leading to increased gaseous by-products such as acetic acid originating fromEA pyrolysis.Notably,DCM at low concentration slightly promoted EA conversion at low temperatures with or without water,consistent with the enhanced EA adsorption in co-adsorption analyses.This was mainly due to that DCM impeded the shielding effect of hydrolysate deposition from rapid EA hydrolysis depending on the decreased acidity.Moreover,water benefited EA hydrolysis but decreased CO_(2) selectivity while the generated water derived from EA was likely to affect DCM transformation.This work may provide theoretical guidance for the promotion of applied catalysts toward industrial applications.
基金supported by the Researchers Supporting Project of King Saud University,Riyadh,Saudi Arabia,under Project RSPD2025R681。
文摘With the continuous advancement of communication and unmanned aerial vehicle(UAV)technologies,the collaborative operations of diverse platforms,including UAVs and ground vehicles,have been significantly promoted.However,battlefield uncertainties,such as equipment failures and enemy attacks,can impact these collaborative operations'stability and communication efficiency.To this end,we design a highly destruction-resistant air-ground cooperative resilient networking platform that aims to enhance the robustness of network communications by integrating ground vehicle information for UAV network deployment.It then incorporates the concept of virtual guiding force,enabling the UAV swarm to adaptively configure its network layout based on ground vehicle information,thereby improving network destruction resistance.Simulation results demonstrate that the UAV swarm involved in the proposed platform exhibits balanced flight energy consumption and excellent performance in network destruction resistance.
基金supported by the National Natural Science Foundation of China(No.2021YFC3200603)the Special Research Assistant Program,Chinese Academy of Sciences.
文摘Photocatalytic disinfection is an eco-friendly strategy for countering bacterial pollution in aquatic environments.Numerous strategies have been devised to facilitate the generation of reactive oxygen species(ROS)within photocatalysts,ultimately leading to the eradication of bacteria.However,the significance of the physical morphology of photocatalysts in the context of sterilization is frequently obscured,and the progress in the development of physical-chemical synergistic sterilization photocatalysts has been relatively limited.Herein,graphitic carbon nitride(g-C_(3)N_(4))is chemically protonated to expose more sharp edges.PL fluorescence and EIS results indicate that the protonation can accelerate photogenerated carrier separation and enhance ROS production.Meanwhile,the sharp edges on the protonated g-C_(3)N_(4)facilitate the physical disruption of cell walls for further promoting oxidative damage.Protonated C_(3)N_(4)demonstrated superior bactericidal performance than that of pristine g-C_(3)N_(4),effectively eliminating Escherichia coli within 40 minutes under irradiation.This work highlights the significance of incorporating physical and chemical synergies in photocatalyst design to enhance the disinfection efficiency of photocatalysis.
文摘BACKGROUND Although laparoscopic gastrolithotomy had been widely used in clinical practice,uncommon postoperative complications still require vigilance by medical staff.CASE SUMMARY Here we report a 67-year-old man who suffered for 18 months and underwent surgery several times due to a rare and undetected complication of laparoscopic gastricolithotomy.He presented to multiple hospitals because of sustained left upper quadrant abdominal pain one month after laparoscopic gastricolithotomy due to a large gastric bezoar caused by unrestrained eating of black dates and was diagnosed with possible intercostal neuritis.Many painkillers were used to relieve his symptoms but the condition progressed.Seven months after surgery,he was hospitalized as skin ulceration occurred in the left upper abdominal wall and was subsequently diagnosed with a massive thoracoabdominal wall abscess.One year after surgery,irreversible costal destruction was demonstrated.Both lesions were finally proved to be secondary damage due to a rare chronic gastro-abdominal wall fistula related to laparoscopic gastricolithotomy and the diameter of the gastric fistula reached 2 centimeters(cm).The patient was ultimately cured but underwent multi-regional incisions and drainage of the abscess,drainage of the gastric fistula,partial gastrectomy and removal of damaged ribs,and was followed-up for more than 4 years without recurrence.It is well-known that gastric fistula usually has an acute onset and occurs early after surgery,while chronic gastro-abdominal wall fistula especially with secondary massive thoracoabdominal wall abscess and costal destruction has rarely been reported.CONCLUSION This may be the first reported case of a chronic thoracoabdominal abscess and costal destruction caused by an undetected chronic gastro-abdominal wall fistula.We believe that this is a novel type of gastric fistula and the diagnosis and treatment were challenging.
文摘Dear Editor,I am Yuki Tanaka from Hakodate Central General Hospital,Japan.Mikulicz’s disease is characterized by symmetrical swelling of lacrimal and salivary glands.In 2012,a Japanese study group proposed comprehensive diagnostic criteria for immunoglobulin G4-related disease(IgG4-RD)^([1]).They have revealed that Mikulicz’s disease is a systemic IgG4-RD and attracted attentions of ophthalmologists.In 2014,the criteria for IgG4-related ophthalmic disease(IgG4-ROD)were established.
文摘In order to improve the destruction efficiency of dioxins and also for developing new dioxin control technology, the destruction mechanisms of 2,3,7,8-tetrachlorodihenzo-p-dioxin （2,3,7,8-TCDD） by O3 and NO3, were investigated employing quantum chemical calculations. For involved reactions, the microcosmic reaction processes were analyzed and depicted in detail based on geometry optimizations made by the B3LYP/6-31G（d） method. At the same time, the reaction activation energies were also calculated at the MP2/6- 311G（d,p）//B3LYP/6-31G（d） level. Configuration analysis indicated that 2,3,7,8-TCDD could be destroyed by 03 and NO3 in two different ways. The destruction of 2,3,7,8-TCDD by 03 proceeded via the addition of 03 and the cleavage of C=C while the destruction of 2,3,7,8-TCDD by NO3 proceeded via the substitution of chlorine by NO3. Calculated results show that, the activation energy of the destruction reaction of 2,3,7,8-TCDD by NO3 （267.48 kJ/mol） is much larger than that of the destruction reaction of 2,3,7,8-TCDD by O3 （51.20 kJ/mol）. This indicated that the destruction of 2,3,7,8-TCDD by 03 is much more efficient than that of 2,3,7,8-TCDD by NO3. The reason why the activation energy for the destruction reaction of 2,3,7,8-TCDD by NO3 is so large, is also discussed.
文摘We aimed to investigate the synergistic effects of ultrasonic waves and repeated freeze-thaw cycles on cel-wal destruction of Auricularia auric-ula mycelia, and determine the best combination of conditions for cel-wal destruc-tion of Auricularia auricula mycelia. [Method] The effects of destruction time, added water, destruction times, freeze time and number of freeze-thaw cycles during ultra-sonic treatment on polysaccharide yield were investigated by single-factor test in our research. The optimum conditions for cel-wal destruction of Auricularia auricula mycelia by the synergistic effect of ultrasonic waves and repeated freeze-thaw cycles were ascertained by orthogonal test. [Result] The results of single test indicated the best combination of conditions was as fol ows： freeze time, 30 min; destruction time, 20 min; added water, 15 times; destruction times, 2 times; number of freeze-thaw cycles, 3 cycles. The results of orthogonal test indicated the influencing factors ranked as destruction time 〉 destruction times 〉 freeze time. The best combination of conditions was as fol ows： freeze time, 30 min; destruction time, 25 min; destruc-tion times, 2 times. Under above conditions, the polysaccharide yield reached 57.76 mg/g. [Conclusion] This research would provide a basis and reference for practical production.
文摘This essay is going to read the different kinds of beings in The Lord of the Rings as different stages of man,and to analyze how they construct or destruct their selves respectively over power.The Lord of the Rings is read as a story about the self-construction and self-destruction of man,who begins to construct the sense of self from a very early stage in life,and becomes who he is in the continuous process of trying to identify himself with the image that keeps changing,of the frequently alternative construction and destruction of his self,and of intersubjectivity,the interrelation with other subjects.
基金Project supported by the National Natural Science Foundation of China(No.50476058)Natural Science Foundation of Zhejiang Province(No.X506214)
文摘PCDD/Fs have been become a serious issue because of their toxicological effects and associated adverse health implications. In this study, the gliding arc plasma was tested for treatment of polychlorinated dibenzo-p-dioxins （PCDDs） and polychlorinated dibenzofurans （PCDFs）, which was synthesized from pentachlorophenol in atmospheric condition at 350℃ with or without the catalysis of CuCl2. From the experiment, we found that the destruction efficiency of PCDD/F homologues after gliding was discharge ranged from 25% to 79%. This result demonstrates that gliding arc plasma is an effective technology to decompose PCDDs/Fs in flue gas. A plausible degradation mechanism for PCDD/Fs by gliding arc was discussed. Finally, a multistage reactor structure of gliding arc was proposed to upgrade removal efficiency for PCDD/Fs.
基金supported by Agency for Defense Development,Korea and Korea Institute of Scienceand Technology
文摘The destruction of methylphosphonic acid （MPA）, a final product by hydrolysis/neutralization of organophosphorus agents such as sarin and VX （O-ethyl S-[2-（diisopropylamino）ethyl] methylphosphonothionate）, was investigated in a a bench-scale, continuous concentric vertical double wall reactor under supercritical water oxidation condition. The experiments were conducted at a temperature range of 450–600°C and a fixed pressure of 25 MPa. Hydrogen peroxide was used as an oxidant. The destruction efficiency （DE） was monitored by analyzing total organic carbon （TOC） and MPA concentrations using ion chromatography on the liquid effluent samples. The results showed that the DE of MPA up to 99.999% was achieved at a reaction temperature of 600°C, oxygen concentration of 113% storichiometric requirement, and reactor residence time of 8 sec. On the basis of the data derived from experiments, a global kinetic rate equation for the DE of MPA and DE of TOC were developed by nonlinear regression analysis. The model predictions agreed well with the experimental data.
文摘Destruction of organic contaminants in water by ozonation is a gas-liquid process which involves ozone mass transfer and fast irreversible chemical reactions. Ozonation reactor design and process optimizing require the modeling of the gas-liquid interactions within the reactor. In this paper a theoretical model combining the fluid dynamic and reaction kinetic parameters is proposed for predicting the destruction rates of organic pollutants in a semi-batch stirred-tank reactor by ozonation. A simple expression for the enhancement factor as our previous work has been applied to evaluate the chemical mass transfer coefficient in ozone absorption. 2,4-dichlorophenol (2,4-DCP) and 2,6-DCP or their mixture are chosen as the model compounds for simulating, and the predicted DCP concentrations are compared with some measured data.
文摘[Objective]To investigate the inhibitory effect ofhonokiol on Candida albicans in vitro.[Methods]The minimum inhibitory concentration(MIC)and minimum fungicidal concentration(MFC)of magnolol for C.albicans were determined by microdilution method.Transmission electron microscopy was used to examine the effect of different concentrations of honokiol on the ultrastructure of C.albicans.Apoptosis of C.albicans caused by honokiol was analyzed by Annexin V-FITC/propidium iodide(PI)double staining and the accumulation of reactive oxygen species(ROS)in C.albicans cells was determined by DCFH-DA staining.The effects of honokiol on mitochondrial membrane potential and cell membrane permeability of C.albicans were analyzed by JC-1 staining and PI staining respectively.[Results]Honokiol had strong inhibitory effect on C.albicans,with MIC90 of 16μg/mL and MFC of 32μg/mL.Honokiol affected cell wall,cell membrane,and cytoplasm of C.albicans,induced both apoptosis and necrosis in C.albicans,probably through ROS production and disruption of mitochondrial function.It also changed the permeability of cell membrane,leaded to the cell wall damage and the binding of ergosterol.[Conclusion]Inhibition of C.albicans by honikiol involves multiple mechanisms including ROS production accompanied by a series of cellular damages.Honikiol appears to be a potential antifungal drug candidate.
文摘In this study, we examined the effects of neuroglobin gene （Ngb） transfection into SH-SY5Y cells, using ultrasound-targeted microbubble destruction （UTMD）, on cobalt chloride-induced hypoxia. With an ultrasound intensity of 0.8 W/cm2, a 60-second exposure duration, 50% duty cycle, and 20% microbubble concentration, pAcGFP1-C1-Ngb-transfected cells exhibited the highest cell viability and transfection efficiency. The efficiency of plasmid delivery was significantly higher with UTMD than transfection with plasmid alone, transfection with plasmid using microbubbles, or transfection of plasmid by ultrasound. In addition, during cobalt chloride-induced hypoxia, caspase-3 activity in pAcGFP1-C1-Ngb-transfected cells was significantly lower than in untransfected cells. Ngb protein and mRNA expression were significantly higher in cells transfected by UTMD than in cells transfected with the other methods. These results demonstrate that UTMD can very efficiently mediate exogenous gene delivery, and that Ngb overexpression protects cells against cobalt chloride-induced hypoxia.
文摘AIM： To detect the therapeutic effects of chemical destruction of celiac ganglion in patients with pancreatic carcinoma with intractable pain. METHODS： Ninety-seven cases with advanced pancreatic carcinoma received chemical destruction of celiac ganglion-5 mL pure alcohol injection around celiac artery under ultrasonic guidance. The changes of visual analogue scale （VAS）, serum substance P （Sub P）, β-endopeptide （β-EP） and T-lymphocyte subtypes level were compared between pre- and post-therapy. RESULTS： Successful rate of puncture was 98.7%, with one failure. No serious complications such as traumatic pancreatitis, pancreatic fistula, abdominal cavity hemorrhage or peritoneal infection occurred. VAS, serum Sub P and β-EP level significantly changed after treatment （8.0 ± 2.3 vs 4.6 ± 2.1, 254.1 ± 96.7 vs 182.4 ± 77.6, 3.2 ± 0.8 vs 8.8 ± 2.1, P 〈 0.01, P 〈 0.05, P 〈 0.01） with complete relief rate 54.2%, partial relief rate 21.9%, ineffective rate 12.5% and recurrent rate 10.7%. The T-lymphocyte subtypes level remarkably increased when compared with that of pre-therapy （46.7 ± 3.7 vs 62.5 ± 5.5, P 〈 0.01）. CONCLUSION： Our study suggests that chemical destruction of celiac ganglion under ultrasonic guidance is highly safe, and can evidently relieve cancer pain and improve the cellular immunity in patients with advanced pancreatic carcinoma.
基金financially supported by the National Natural Science Foundation of China[No.81372937]
文摘The molecular pathogenesis of T-2 toxin-induced cartilage destruction has not been fully unraveled yet. The aim of this study was to detect changes in serum metabolites in a rat anomaly model with articular cartilage destruction. Thirty healthy male Wistar rats were fed a diet containing T-2 toxin （300 ng/kg chow） for 3 months. Histopathological changes in femorotibial cartilage were characterized in terms of chondrocyte degeneration/necrosis and superficial cartilage defect, and the endogenous metabolite profile of serum was determined by UPLC/Q-TOF MS. Treated rats showed extensive areas of chondrocyte necrosis and superficial cartilage defect in the articular cartilage. In addition, 8 metabolites were found to change significantly in these rats compared to the control group, including lyso PE （18：0/0：0）, lyso PC（14：0）, lyso PC[18：4 （6Z,9Z,12Z,15Z）], lyso PC[（16：1（9Z）], lyso PC（16：0）, L-valine, hippuric acid, and asparaginyl-glycine. These 8 metabolites associated with cartilage injury are mainly involved in phospholipid and amino acid metabolic pathways.
基金This work was financially supported by the National Key R&D Program of China(No.2016YFC0600109)the State Key Laboratory of Lithospheric Evolution,IGGCAS(No.SKL-Z201905)+1 种基金This work was also financially supported by the National Natural Science Foundation of China(No.41602099)the State Key Laboratory of Lithospheric Evolution,IGGCAS(No.SPECIAL201606).
文摘The North China Craton(NCC)is the best example of an Archean craton that has lost its stability in the Late Mesozoic.Although the cratonic destruction is generally considered to have occurred in the Eastern Block and reached a peak in the Early Cretaceous,the exact areal extent of cratonic destruction is debated,especially the southern and northern margin of the NCC.Here we report geochronology,geochemical and Hf isotopic data of the Late Mesozoic granitoids from Lushi polymetal mineralization area(LPMA)in the southern margin of NCC.These results provide new insights into the destruction in the southern margin of the NCC during the Late Mesozoic.Zircon U-Pb dating indicates that eight granitoids intruded in the Late Jurassic to Early Cretaceous(136.8–154.1 Ma),respectively.Geochemical signatures define these granitoids being A-type or I-type granite that formed in an extension setting.In addition,Hf isotopic compositions of zircons from these granitoids vary in a relatively large range,withεHf(t)values and TDM2 ages ranginge from-26.1 to+15.2 and 215 to 2849 Ma,respectively.The parental magmas were likely derived from diverse sources,including materials of the partial melting of ancient lower crust and mantle-derived mafic magmas in various proportions.Combining with previous studies on the contemporaneous magma-tectonic activities in circum of NCC,we suggest that the rim of NCC was already unstabilized from the Late Jurassic in the LPMA.The subduction of the Paleo-Pacific Plate was the main trigger to the destruction of the southern margin of NCC,which was responsible for the lithospheric extension and thinning,extensive magmatism and mineralization.
基金supported in part by the Japan Agency for Medical Research and Development (AMED) under grant number JP20ek0410073, JP23ek0410108, JP22ek0410100, AMEDCREST under grant number JP19gm1210008 and AMED-PRIME under grant number JP21gm6310029, the AMED Japan Initiative for World leading Vaccine Research and Development Centers (JP223fa627001)Japan Society for the Promotion of Science (JSPS): Scientific Research S (21H05046), Scientific Research B (21H03104, 22H03195, and 22H02844) and Challenging Research (20K21515 and 21K18254)+3 种基金the JST FOREST Program (JPMJFR2261, JPMJFR205Z)Y.A. was supported by a JSPS Research Fellowship for Young Scientists (23KJ1949)Japanese Society for Immunology (JSI)Kibou Scholarship for Doctoral Students in Immunology。
文摘The immune-stromal cell interactions play a key role in health and diseases. In periodontitis, the most prevalent infectious disease in humans, immune cells accumulate in the oral mucosa and promote bone destruction by inducing receptor activator of nuclear factor-κB ligand (RANKL) expression in osteogenic cells such as osteoblasts and periodontal ligament cells. However, the detailed mechanism underlying immune–bone cell interactions in periodontitis is not fully understood. Here, we performed single-cell RNAsequencing analysis on mouse periodontal lesions and showed that neutrophil–osteogenic cell crosstalk is involved in periodontitis-induced bone loss. The periodontal lesions displayed marked infiltration of neutrophils, and in silico analyses suggested that the neutrophils interacted with osteogenic cells through cytokine production. Among the cytokines expressed in the periodontal neutrophils, oncostatin M (OSM) potently induced RANKL expression in the primary osteoblasts, and deletion of the OSM receptor in osteogenic cells significantly ameliorated periodontitis-induced bone loss. Epigenomic data analyses identified the OSM-regulated RANKL enhancer region in osteogenic cells, and mice lacking this enhancer showed decreased periodontal bone loss while maintaining physiological bone metabolism. These findings shed light on the role of neutrophils in bone regulation during bacterial infection, highlighting the novel mechanism underlying osteoimmune crosstalk.
