Sensors capable of recognizing cell surface carbohydrates,such as sialyl Lewis X（sLe-x）,are invaluable research tools and for the diagnosis and early detection of many forms of cancer.In this paper,we report the des...Sensors capable of recognizing cell surface carbohydrates,such as sialyl Lewis X（sLe-x）,are invaluable research tools and for the diagnosis and early detection of many forms of cancer.In this paper,we report the design and synthesis of a series of bisboronic acids 6（a-f） as fluorescent sensors towards mono-/oligosaccharides.Among them,compounds 6d and 6e showed strong binding affinities with glucose and fructose,while compound 6c,in which two anthracene-based boronic acid units were linked by a hexamethylene spacer,was able to recognize sLe-x selectivity and stained HEPG2 cells at 1 μmoI/L.展开更多
Lanthanide-doped upconversion nanoparticles(Ln-UCNPs)are a new type of nanomaterials with excellent fluorescence properties,which are well applied in fluorescent biosensing.Herein we developed a multifunctional probe ...Lanthanide-doped upconversion nanoparticles(Ln-UCNPs)are a new type of nanomaterials with excellent fluorescence properties,which are well applied in fluorescent biosensing.Herein we developed a multifunctional probe based on the surface engineering of core-shell structure UCNPs with polyacrylic acid(PAA).The developed PAA/UCNPs probe could be highly selective to detect and respond to Cu^(2+) at different pH.Cu^(2+) could easily combine with the carboxylate anion of PAA to quench the fluorescence of UCNPs.Therefore,we creatively proposed a fluorescent array sensor(PAA/UCNPs-Cu^(2+)),in which the same material acted as the sensing element by coupled with pH regulation for pattern recognition of 5 thiols.It could also easily identify the chiral enantiomer of cystine(L-Cys-and D-Cys),and distinguish their mixed samples with different concentrations,and more importantly,it could be combined with urine samples to detect actual level of homocysteine(Hcys)to provide a new solution for judging whether the human body suffers from homocystinuria.展开更多
Supraparticles(SPs),such as assembly of inorganic components with organic,have made tremendous attention in biochemical analysis,which represents a novel but challenging research orientation.Herein,a single-SPs multif...Supraparticles(SPs),such as assembly of inorganic components with organic,have made tremendous attention in biochemical analysis,which represents a novel but challenging research orientation.Herein,a single-SPs multifunctional fluorescent sensor array has been developed for high-throughput detection of heavy metal ions in biofluids,which is based on an inorganic/organic hybrid SPs consisting of carbon dots(CDs)and an easily available porphyrin[5,10,15,20-tetra(4-carboxyphenyl)porphyrin(TCPP)].TCPP can aggregate with the CDs to form the assembly(CDs/TCPP SPs)through the electrostatic andπ-πstacking interaction.There are two independent and clearly separated fluorescence emission peaks at 470 and 668 nm in the resultant CDs/TCPP SPs under 380 nm excitation.As a proof-of concept design,F 470,F 668,F 668/F 470 of SPs are chosen as three sensor components to constitute our sensor array.With the addi-tion of metal ions,three sensor components can generate different fluorescence response patterns for discriminating 11 heavy metal ions via principal component analysis(PCA).Additionally,thiols can read-ily capture Cu^(2+)to switch the fluorescence of CDs/TCPP initially altered by Cu^(2+).Hence,CDs/TCPP-Cu^(2+)ensemble is further demonstrated to be a powerful sensor array for pattern recognition of 7 thiols and even chiral recognition of cysteine enantiomers.This novel strategy avoids the tanglesome synthesis of multiple sensing probes and dedicates an innovative method for the facile establishment of tongue-mimic sensors,which would prospectively sprout more homologous assumptions to broaden its application toward more biosensing fields.展开更多
For a vision measurement system consisted of laser-CCD scanning sensors, an algorithm is proposed to extract and recognize the target object contour. Firstly, the two-dimensional（2D） point cloud that is output by th...For a vision measurement system consisted of laser-CCD scanning sensors, an algorithm is proposed to extract and recognize the target object contour. Firstly, the two-dimensional（2D） point cloud that is output by the integrated laser sensor is transformed into a binary image. Secondly, the potential target object contours are segmented and extracted based on the connected domain labeling and adaptive corner detection. Then, the target object contour is recognized by improved Hu invariant moments and BP neural network classifier. Finally, we extract the point data of the target object contour through the reverse transformation from a binary image to a 2D point cloud. The experimental results show that the average recognition rate is 98.5% and the average recognition time is 0.18 s per frame. This algorithm realizes the real-time tracking of the target object in the complex background and the condition of multi-moving objects.展开更多
Triboelectricity-driven acoustic transducers with various merits have demonstrated significant potential in energy harvesting and self-powered sensing.The transducers generally require additionally a spacer and a corr...Triboelectricity-driven acoustic transducers with various merits have demonstrated significant potential in energy harvesting and self-powered sensing.The transducers generally require additionally a spacer and a corresponding exquisite process for smooth operation,which provides an unnecessary interface between the elements.The exploration of a novel manufacturing approach for triboelectricity-driven acoustic transducers is warranted to resolve this issue.Here,Triboelectricitydriven Oscillating Nano-Electricity generator(TONE)developed via mechanically guided fourdimensional(4D)printing is introduced for acoustic energy harvesting and self-powered voice recognition.The mechanically buckled structure of the TONE facilitates its smooth oscillation by sound wave without the use of an additional spacer,enabling the TONE to exhibit outputs of 156 V and 10μA.The output characteristics of the TONE are analyzed based on the acoustic-structuraltriboelectric interaction mechanism.The TONE demonstrates practical versatility by providing power to commercial electronics from controlled/daily sound and being utilized in artificial intelligence-based human voice recognition sensors.展开更多
基金supported by the National Natural Science Foundation of China(NSFC)(No.21372060)Hebei Province Natural Science Fund for Distinguished Young Scholars(No.B2015201005)
文摘Sensors capable of recognizing cell surface carbohydrates,such as sialyl Lewis X（sLe-x）,are invaluable research tools and for the diagnosis and early detection of many forms of cancer.In this paper,we report the design and synthesis of a series of bisboronic acids 6（a-f） as fluorescent sensors towards mono-/oligosaccharides.Among them,compounds 6d and 6e showed strong binding affinities with glucose and fructose,while compound 6c,in which two anthracene-based boronic acid units were linked by a hexamethylene spacer,was able to recognize sLe-x selectivity and stained HEPG2 cells at 1 μmoI/L.
基金supported by the National Natural Science Foundation of China(No.21775044)the Shanghai Science and Technology Committee(Nos.19ZR1473300 and 18DZ1112700)the Fundamental Research Funds for the Central Universities。
文摘Lanthanide-doped upconversion nanoparticles(Ln-UCNPs)are a new type of nanomaterials with excellent fluorescence properties,which are well applied in fluorescent biosensing.Herein we developed a multifunctional probe based on the surface engineering of core-shell structure UCNPs with polyacrylic acid(PAA).The developed PAA/UCNPs probe could be highly selective to detect and respond to Cu^(2+) at different pH.Cu^(2+) could easily combine with the carboxylate anion of PAA to quench the fluorescence of UCNPs.Therefore,we creatively proposed a fluorescent array sensor(PAA/UCNPs-Cu^(2+)),in which the same material acted as the sensing element by coupled with pH regulation for pattern recognition of 5 thiols.It could also easily identify the chiral enantiomer of cystine(L-Cys-and D-Cys),and distinguish their mixed samples with different concentrations,and more importantly,it could be combined with urine samples to detect actual level of homocysteine(Hcys)to provide a new solution for judging whether the human body suffers from homocystinuria.
基金supported by the National Natural Science Foun-dation of China(No.21775044)the Shanghai Science and Tech-nology Committee(Nos.19ZR1473300,18DZ1112700)+1 种基金the Shanghai Key Laboratory of Multidimensional Information Processing(No.MIP202104)the Fundamental Research Funds for the Central Universities.
文摘Supraparticles(SPs),such as assembly of inorganic components with organic,have made tremendous attention in biochemical analysis,which represents a novel but challenging research orientation.Herein,a single-SPs multifunctional fluorescent sensor array has been developed for high-throughput detection of heavy metal ions in biofluids,which is based on an inorganic/organic hybrid SPs consisting of carbon dots(CDs)and an easily available porphyrin[5,10,15,20-tetra(4-carboxyphenyl)porphyrin(TCPP)].TCPP can aggregate with the CDs to form the assembly(CDs/TCPP SPs)through the electrostatic andπ-πstacking interaction.There are two independent and clearly separated fluorescence emission peaks at 470 and 668 nm in the resultant CDs/TCPP SPs under 380 nm excitation.As a proof-of concept design,F 470,F 668,F 668/F 470 of SPs are chosen as three sensor components to constitute our sensor array.With the addi-tion of metal ions,three sensor components can generate different fluorescence response patterns for discriminating 11 heavy metal ions via principal component analysis(PCA).Additionally,thiols can read-ily capture Cu^(2+)to switch the fluorescence of CDs/TCPP initially altered by Cu^(2+).Hence,CDs/TCPP-Cu^(2+)ensemble is further demonstrated to be a powerful sensor array for pattern recognition of 7 thiols and even chiral recognition of cysteine enantiomers.This novel strategy avoids the tanglesome synthesis of multiple sensing probes and dedicates an innovative method for the facile establishment of tongue-mimic sensors,which would prospectively sprout more homologous assumptions to broaden its application toward more biosensing fields.
文摘For a vision measurement system consisted of laser-CCD scanning sensors, an algorithm is proposed to extract and recognize the target object contour. Firstly, the two-dimensional（2D） point cloud that is output by the integrated laser sensor is transformed into a binary image. Secondly, the potential target object contours are segmented and extracted based on the connected domain labeling and adaptive corner detection. Then, the target object contour is recognized by improved Hu invariant moments and BP neural network classifier. Finally, we extract the point data of the target object contour through the reverse transformation from a binary image to a 2D point cloud. The experimental results show that the average recognition rate is 98.5% and the average recognition time is 0.18 s per frame. This algorithm realizes the real-time tracking of the target object in the complex background and the condition of multi-moving objects.
基金supported by the National Research Foundation of Korea(NRF)grant funded by the Korea government(MSIT)(No.RS-2024-00344920)supported by the Human Resources Development of the Korea Institute of Energy Technology Evaluation and Planning(KETEP)grant funded by the Ministry of Trade,Industry and Energy of Korea(No.RS-2023-00244330)。
文摘Triboelectricity-driven acoustic transducers with various merits have demonstrated significant potential in energy harvesting and self-powered sensing.The transducers generally require additionally a spacer and a corresponding exquisite process for smooth operation,which provides an unnecessary interface between the elements.The exploration of a novel manufacturing approach for triboelectricity-driven acoustic transducers is warranted to resolve this issue.Here,Triboelectricitydriven Oscillating Nano-Electricity generator(TONE)developed via mechanically guided fourdimensional(4D)printing is introduced for acoustic energy harvesting and self-powered voice recognition.The mechanically buckled structure of the TONE facilitates its smooth oscillation by sound wave without the use of an additional spacer,enabling the TONE to exhibit outputs of 156 V and 10μA.The output characteristics of the TONE are analyzed based on the acoustic-structuraltriboelectric interaction mechanism.The TONE demonstrates practical versatility by providing power to commercial electronics from controlled/daily sound and being utilized in artificial intelligence-based human voice recognition sensors.
