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Fosl-1对肾母细胞瘤动物模型中Wnt通路及肿瘤转移相关因子的影响
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作者 海力其古丽·努日丁 古丽巴哈·买买提 +2 位作者 刘玉 王学梅 严媚 《新疆医科大学学报》 2026年第2期198-204,共7页
目的探讨Fosl-1、Wnt通路及肿瘤转移相关因子与肾母细胞瘤肿瘤转移的相关性,为寻找肾母细胞瘤治疗靶点提供理论依据。方法将慢病毒Fosl-1干扰的WIT49肾母细胞瘤细胞及Fosl-1干扰空载的WIT49细胞分别皮下接种于BALB/c-Nude雄性裸鼠以构... 目的探讨Fosl-1、Wnt通路及肿瘤转移相关因子与肾母细胞瘤肿瘤转移的相关性,为寻找肾母细胞瘤治疗靶点提供理论依据。方法将慢病毒Fosl-1干扰的WIT49肾母细胞瘤细胞及Fosl-1干扰空载的WIT49细胞分别皮下接种于BALB/c-Nude雄性裸鼠以构建移植瘤模型。实验动物分为4组:对照组(皮下接种未处理的WIT49细胞)、Fosl-1干扰-NC组(皮下接种空载慢病毒转染的WIT49细胞)、Fosl-1干扰组(皮下接种Fosl-1干扰慢病毒转染的WIT49细胞)和Fosl-1干扰+Wnt抑制组(皮下接种Fosl-1干扰慢病毒转染的WIT49细胞,在接种后进行腹腔注射Wnt抑制剂FH535)。观察肿瘤生长并测量瘤体体积;采用实时荧光定量逆转录聚合酶链式反应(qRT-PCR)检测Fosl-1 mRNA表达;通过蛋白质免疫印迹(Western blot)检测Wnt通路关键蛋白[卷曲蛋白1(FZD1)、Wnt家族成员1(Wnt-1)、β-连环蛋白(β-catenin)]、肿瘤转移相关蛋白[基质金属蛋白酶9(MMP-9)、肿瘤转移抑制蛋白23(NM23)]及促凋亡蛋白Bax的表达;运用HE染色及免疫组织化学法观察肿瘤组织形态并检测Fosl-1蛋白表达。结果与对照组相比,Fosl-1干扰组和Fosl-1干扰+Wnt抑制组裸鼠肿瘤体积在第13天和第16天均减小(P<0.05)。在接种细胞后第10天、第13天和第16天,与Fosl-1干扰组相比,Fosl-1干扰+Wnt抑制组裸鼠的肿瘤体积均更小,差异有统计学意义(P<0.05)。与对照组相比,Fosl-1干扰组和Fosl-1干扰+Wnt抑制组的Fosl-1 mRNA、Wnt信号通路相关关键蛋白FZD1、Wnt-1、β-catenin及肿瘤转移相关蛋白MMP-9、NM23的表达均下降,而促凋亡蛋白Bax的表达升高(P<0.05)。HE染色结果显示,与对照组相比,Fosl-1干扰组及Fosl-1干扰+Wnt抑制组肿瘤细胞核分裂像减少,核浆比降低,间质结构更疏松,同时,空泡状细胞结构增加,尤其Fosl-1干扰+Wnt抑制组空泡状细胞结构增加更明显。免疫组化检测结果显示,与对照组相比,Fosl-1干扰组和Fosl-1干扰+Wnt抑制组中Fosl-1蛋白的表达量均降低,差异有统计学意义(P<0.05)。结论抑制Fosl-1能有效延缓肾母细胞瘤的生长并降低肿瘤转移相关因子的表达。联合抑制Wnt通路能进一步增强对肿瘤生长的抑制作用,提示Fosl-1在肾母细胞瘤中的作用可能依赖于Wnt/β-catenin信号通路。 展开更多
关键词 fosl-1 肾母细胞瘤 WNT通路 肿瘤转移
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lncRNA XIST调控miR-17-5p/FOSL1轴对胆囊癌细胞增殖、迁移和侵袭的影响
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作者 陈李康 姚磊 彭莹莹 《肝胆胰外科杂志》 2026年第2期115-123,共9页
目的探讨长链非编码RNA(lncRNA)X染色体失活特异性转录本(XIST)调控微小RNA-17-5p(miR-17-5p)/FOS样抗原1(FOSL1)轴对胆囊癌(GBC)细胞增殖、迁移和侵袭的影响。方法qRT-PCR法检测GBC组织和细胞株中mRNA表达;双荧光素酶报告基因实验验证l... 目的探讨长链非编码RNA(lncRNA)X染色体失活特异性转录本(XIST)调控微小RNA-17-5p(miR-17-5p)/FOS样抗原1(FOSL1)轴对胆囊癌(GBC)细胞增殖、迁移和侵袭的影响。方法qRT-PCR法检测GBC组织和细胞株中mRNA表达;双荧光素酶报告基因实验验证lncRNA XIST与miR-17-5p,miR-17-5p与FOSL1的互作;将EH-GB1细胞分为Ctrl组、sh-NC组、sh-XIST组、sh-XIST+inhibitor-NC组、sh-XIST+inhibitor-miR-17-5p组、mimic-NC组、mimic-miR-17-5p组、mimic-miR-17-5p+OE-NC组、mimic-miR-17-5p+OE-FOSL1组。台盼蓝染色和平板克隆实验检测EH-GB1细胞增殖;划痕试验检测EH-GB1细胞迁移;Transwell实验检测EH-GB1细胞侵袭;Western blotting检测EH-GB1细胞中Ki67、Cyclin D1、MMP-2、CD44、FOSL1蛋白的表达。裸鼠移植瘤实验检测敲低lncRNA XIST对GBC肿瘤生长的影响。结果GBC组织和细胞中miR-17-5p呈低表达,lncRNA XIST、FOSL1呈高表达。sh-XIST组细胞存活率、增殖数、划痕愈合率、侵袭数,Ki67、Cyclin D1、MMP-2、CD44表达水平低于sh-NC组、Ctrl组(P<0.05);sh-XIST+inhibitor-miR-17-5p组细胞存活率、增殖数、划痕愈合率、侵袭数及Ki67、Cyclin D1、MMP-2、CD44表达水平高于sh-XIST组、sh-XIST+inhibitor-NC组(P<0.05)。mimic-miR-17-5p组细胞存活率、增殖数、划痕愈合率、侵袭数及Ki67、Cyclin D1、MMP-2、CD44、FOSL1表达水平低于mimic-NC组、Ctrl组(P<0.05);mimic-miR-17-5p+OE-FOSL1组细胞存活率、增殖数、划痕愈合率、侵袭数及Ki67、Cyclin D1、MMP-2、CD44、FOSL1表达水平高于mimic-miR-17-5p组、mimic-miR-17-5p+OE-NC组(P<0.05)。lncRNA XIST可以靶向负调控miR-17-5p,而miR-17-5p可以靶向负调控FOSL1(P<0.05)。XIST敲低组裸鼠移植瘤体积、体质量,肿瘤FOSL1蛋白、lncRNA XIST表达水平低于阴性对照组,miR-17-5p表达水平高于阴性对照组(P<0.05)。结论lncRNA XIST通过调控miR-17-5p/FOSL1轴促进GBC细胞的增殖、迁移和侵袭。 展开更多
关键词 长链非编码RNA X染色体失活特异性转录本 微小RNA-17-5p fos样抗原1 胆囊癌 增殖 迁移 侵袭
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和胃安神方对失眠模型大鼠视交叉上核c-fos、c-jun及神经元型一氧化氮合酶表达的影响
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作者 刘子涵 王硕 +7 位作者 邹涛 田楚娇 于泓洋 底昂 王亮 陈琦 王长振 李绍旦 《环球中医药》 2026年第1期36-44,共9页
目的探讨和胃安神方对失眠模型大鼠视交叉上核(suprachiasmatic nucleus,SCN)即刻早期基因c-fos、c-jun以及神经元型一氧化氮合酶(neuronal nitric oxide synthase,nNOS)表达的影响。方法选用60只雄性SD大鼠,随机分配至正常对照组、模... 目的探讨和胃安神方对失眠模型大鼠视交叉上核(suprachiasmatic nucleus,SCN)即刻早期基因c-fos、c-jun以及神经元型一氧化氮合酶(neuronal nitric oxide synthase,nNOS)表达的影响。方法选用60只雄性SD大鼠,随机分配至正常对照组、模型对照组、艾司唑仑组以及和胃安神方低、中、高剂量组,每组10只。除正常对照组外,其余各组大鼠均通过腹腔注射对氯苯丙氨酸诱导失眠模型。造模效果通过戊巴比妥钠翻正实验进行评估。造模成功后,各组大鼠接受连续7天的灌胃给药:正常对照组与模型对照组灌服10 mL/kg蒸馏水;艾司唑仑组给予0.33 mg/(kg·d)艾司唑仑水溶液;和胃安神方低、中、高剂量组则分别灌胃浓度为1 g/mL、2 g/mL、4 g/mL的和胃安神方药液,给药体积均为10 mL/kg。给药7天完成后,开展旷场实验及高架十字迷宫实验以评估大鼠行为并采用免疫组化法和qPCR法检测大鼠SCN中c-fos、c-jun以及nNOS表达情况。结果造模结束后,与正常对照组相比,造模组大鼠睡眠潜伏期延长(P<0.01),睡眠时间缩短(P<0.01)。末次给药后,与正常对照组相比,模型对照组大鼠旷场运动总路程、平均速度显著增加(P<0.01),旷场中央区停留时间显著下降(P<0.01),高架十字迷宫开放臂进入次数比例、开放臂停留时间比例下降(P<0.01),SCN中c-fos、c-jun及nNOS的表达水平显著升高(P<0.01)。与模型对照组相比,艾司唑仑组与和胃安神方中、高剂量组大鼠旷场运动总路程、平均速度显著下降(P<0.05或P<0.01),旷场中央区停留时间显著上升(P<0.05或P<0.01);艾司唑仑组与和胃安神方低、中、高剂量组大鼠高架十字迷宫进入开放臂次数比例、开放臂停留时间比例均升高(P<0.05或P<0.01),SCN中c-fos、c-jun及nNOS的表达水平显著降低(P<0.05或P<0.01)。结论和胃安神方能有效缓解失眠模型大鼠的睡眠障碍,可能与其对SCN中c-fos、c-jun及nNOS表达水平的下调作用相关。 展开更多
关键词 失眠 和胃安神方 视交叉上核 c-fos c-jun 神经元型一氧化氮合酶
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Noninvasive Radar Sensing Augmented with Machine Learning for Reliable Detection of Motor Imbalance
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作者 Faten S.Alamri Adil Ali Saleem +2 位作者 Muhammad I.Khan Hafeez Ur Rehman Siddiqui Amjad Rehman 《Computer Modeling in Engineering & Sciences》 2026年第1期698-726,共29页
Motor imbalance is a critical failure mode in rotating machinery,potentially causing severe equipment damage if undetected.Traditional vibration-based diagnostic methods rely on direct sensor contact,leading to instal... Motor imbalance is a critical failure mode in rotating machinery,potentially causing severe equipment damage if undetected.Traditional vibration-based diagnostic methods rely on direct sensor contact,leading to installation challenges and measurement artifacts that can compromise accuracy.This study presents a novel radar-based framework for non-contact motor imbalance detection using 24 GHz continuous-wave radar.A dataset of 1802 experimental trials was sourced,covering four imbalance levels(0,10,20,30 g)across varying motor speeds(500–1500 rpm)and load torques(0–3 Nm).Dual-channel in-phase and quadrature radar signals were captured at 10,000 samples per second for 30-s intervals,preserving both amplitude and phase information for analysis.A multi-domain feature extraction methodology captured imbalance signatures in time,frequency,and complex signal domains.From 65 initial features,statistical analysis using Kruskal–Wallis tests identified significant descriptors,and recursive feature elimination with Random Forest reduced the feature set to 20 dimensions,achieving 69%dimensionality reduction without loss of performance.Six machine learning algorithms,Random Forest,Extra Trees Classifier,Extreme Gradient Boosting,Categorical Boosting,Support Vector Machine with radial basis function kernel,and k-Nearest Neighbors were evaluated with grid-search hyperparameter optimization and five-fold cross-validation.The Extra Trees Classifier achieved the best performance with 98.52%test accuracy,98%cross-validation accuracy,and minimal variance,maintaining per-class precision and recall above 97%.Its superior performance is attributed to its randomized split selection and full bootstrapping strategy,which reduce variance and overfitting while effectively capturing the nonlinear feature interactions and non-normal distributions present in the dataset.The model’s average inference time of 70 ms enables near real-time deployment.Comparative analysis demonstrates that the radar-based framework matches or exceeds traditional contact-based methods while eliminating their inherent limitations,providing a robust,scalable,and noninvasive solution for industrial motor condition monitoring,particularly in hazardous or space-constrained environments. 展开更多
关键词 Condition monitoring imbalance detection industrial applications machine learning motor fault diagnosis non-contact sensing radar sensing vibration monitoring
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Azobenzene-winged phenanthroline for supramolecular chirality sensing and multidimensional chiroptical manipulation via solvent,light,temperature,and redox
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作者 Xiaoqian Wang Yanling Shen +6 位作者 Long Chen Lizhi Fang Kuppusamy Kanagaraj Ming Rao Chunying Fan Wanhua Wu Cheng Yang 《Chinese Chemical Letters》 2026年第2期453-457,共5页
Azobenzene-winged phenanthrolines(L1 and L2)were designed,synthesized,and fully characterized.Ligand L1 forms an in-situ cobalt complex,which has been effectively employed as a circular dichroism(CD)-active chiral sen... Azobenzene-winged phenanthrolines(L1 and L2)were designed,synthesized,and fully characterized.Ligand L1 forms an in-situ cobalt complex,which has been effectively employed as a circular dichroism(CD)-active chiral sensor.The resulting ternary complex(L1-Co^(2+)-amino alcohol)exhibits pronounced exciton-coupled circular dichroism(ECCD)signals at the characteristic azobenzene absorption bands.These signals arise from efficient chirality transfer from the chiral amino alcohol to the azobenzene chromophores,enabling the determination of the absolute configuration of chiral amino alcohols.Accordingly,the L1-Co^(2+)coordination system demonstrates considerably potential in chirality sensing applications.Remarkably,the induced ECCD signals are highly responsive to multiple external stimuli,including photoirradiation,solvent polarity,temperature,and redox conditions.In particular,temperature and redox changes can induce a reversible inversion of the ECCD signal,thereby establishing this system as a multifunctional,stimuli-responsive chiroptical molecular switch. 展开更多
关键词 Phenanthroline derivative AZOBENZENE Amino alcohols Chirality sensing Stimuli-response
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Enhancing the estimation of soil water content using a resistive heater in the dual-probe distributed temperature sensing approach
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作者 Luis Eduardo Bertotto Alan Reis +1 位作者 Cristina de Hollanda Cavalcanti Tsuha Edson Cezar Wendland 《Journal of Rock Mechanics and Geotechnical Engineering》 2026年第1期625-636,共12页
The dual-probe heat pulse(DPHP)is a well-established method for estimating soil moisture(θ)using soil thermal conductivity(λ)and volumetric heat capacity(C_(v)).Recently,monitoringθhas been improved by integrating ... The dual-probe heat pulse(DPHP)is a well-established method for estimating soil moisture(θ)using soil thermal conductivity(λ)and volumetric heat capacity(C_(v)).Recently,monitoringθhas been improved by integrating the DPHP method with distributed temperature sensing(DTS)technology.In the DPHP-DTS approach,a single fiber optic(FO)cable with embedded metallic constituents functions as a heating element,while a parallel cable serves to monitor the temperature.Despite ongoing advancements,challenges such as the difficulty in positioning heating and sensing cables and high energy requirements hinder the widespread adoption of the DPHP-DTS method.As alternative heating materials are seldom used,this study evaluated the feasibility of employing a resistive metallic alloy as the heating element in a laboratory DPHP-DTS application.Overall,higher errors were observed when assessing C_(v)andλat higherθvalues(>0.2),but using C_(v)data produced more accurateθestimates(with the root mean square error(RMSE)≤0.06).Based on C_(v)values,a low-power,long-duration heat pulse(8.07 W/m for 300 s)yielded more consistentθestimates(RMSE=0.04)than a high-power,shortduration pulse(15.93 W/m for 180 s,with RMSE=0.06).The findings of this study also indicated that variations in heating uniformity and electrical power fluctuations potentially affected measurement accuracy.Nevertheless,the resistive alloy proved advantageous for DPHP-DTS due to its independent power connection,ability to maintain linear positioning within the soil,and potential for energy savings,all while providing reliableθestimates. 展开更多
关键词 Fiber optic(FO) Heat pulse Heating material Soil thermal properties Soil moisture
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GLMCNet: A Global-Local Multiscale Context Network for High-Resolution Remote Sensing Image Semantic Segmentation
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作者 Yanting Zhang Qiyue Liu +4 位作者 Chuanzhao Tian Xuewen Li Na Yang Feng Zhang Hongyue Zhang 《Computers, Materials & Continua》 2026年第1期2086-2110,共25页
High-resolution remote sensing images(HRSIs)are now an essential data source for gathering surface information due to advancements in remote sensing data capture technologies.However,their significant scale changes an... High-resolution remote sensing images(HRSIs)are now an essential data source for gathering surface information due to advancements in remote sensing data capture technologies.However,their significant scale changes and wealth of spatial details pose challenges for semantic segmentation.While convolutional neural networks(CNNs)excel at capturing local features,they are limited in modeling long-range dependencies.Conversely,transformers utilize multihead self-attention to integrate global context effectively,but this approach often incurs a high computational cost.This paper proposes a global-local multiscale context network(GLMCNet)to extract both global and local multiscale contextual information from HRSIs.A detail-enhanced filtering module(DEFM)is proposed at the end of the encoder to refine the encoder outputs further,thereby enhancing the key details extracted by the encoder and effectively suppressing redundant information.In addition,a global-local multiscale transformer block(GLMTB)is proposed in the decoding stage to enable the modeling of rich multiscale global and local information.We also design a stair fusion mechanism to transmit deep semantic information from deep to shallow layers progressively.Finally,we propose the semantic awareness enhancement module(SAEM),which further enhances the representation of multiscale semantic features through spatial attention and covariance channel attention.Extensive ablation analyses and comparative experiments were conducted to evaluate the performance of the proposed method.Specifically,our method achieved a mean Intersection over Union(mIoU)of 86.89%on the ISPRS Potsdam dataset and 84.34%on the ISPRS Vaihingen dataset,outperforming existing models such as ABCNet and BANet. 展开更多
关键词 Multiscale context attention mechanism remote sensing images semantic segmentation
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Modulation of quorum sensing,biofilm formation,and migration of Vibrio harveyi by curcumin-mediated photo/sonodynamic treatment
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作者 Dehua Wang Fang Xu +4 位作者 Feng Zhou Jiamiao Hu Yi Zhang Natthida Sriboonvorakul Shaoling Lin 《Journal of Future Foods》 2026年第6期1188-1194,共7页
Vibrio harveyi,a Gram-negative bacterium ubiquitous in marine environments,is recognized as an opportunistic pathogen affecting various aquatic organisms such as fish,shrimp,and shellfish.To enhance its environmental ... Vibrio harveyi,a Gram-negative bacterium ubiquitous in marine environments,is recognized as an opportunistic pathogen affecting various aquatic organisms such as fish,shrimp,and shellfish.To enhance its environmental resilience and adaptive capacity,V.harveyi employs a complex quorum sensing mechanism to modulate its virulence factors,such as bioluminescence,biofilm formation,and motility.Therefore,targeting the quorum sensing of V.harveyi could be a promising strategy to develop novel approaches to ensure the microbial safety of seafood products.This study aims to evaluate the impact of curcumin-mediated photo/sonodynamic treatment on quorum sensing in V.harveyi and its regulated functions.The results indicate a significant decrease of luminescence in V.harveyi following curcumin-mediated photo/sonodynamic treatment.Correspondingly,the biofilm formation ability and bacterial motility of V.harveyi were also greatly impaired by the treatment.Notably,the production of reactive oxygen species in bacteria induced by the photo/sonodynamic treatment could be the underlying mechanism involved in the observed disruption of quorum sensing.These findings underscore the great potential of photo/sonodynamic treatment as a promising strategy to disrupt quorum sensing and mitigate the virulence of V.harveyi,thereby contributing to the development of effective control strategies against this pervasive pathogen. 展开更多
关键词 Photo/sonodynamic treatment Vibrio harveyi Quorum sensing CURCUMIN
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MewCDNet: A Wavelet-Based Multi-Scale Interaction Network for Efficient Remote Sensing Building Change Detection
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作者 Jia Liu Hao Chen +5 位作者 Hang Gu Yushan Pan Haoran Chen Erlin Tian Min Huang Zuhe Li 《Computers, Materials & Continua》 2026年第1期687-710,共24页
Accurate and efficient detection of building changes in remote sensing imagery is crucial for urban planning,disaster emergency response,and resource management.However,existing methods face challenges such as spectra... Accurate and efficient detection of building changes in remote sensing imagery is crucial for urban planning,disaster emergency response,and resource management.However,existing methods face challenges such as spectral similarity between buildings and backgrounds,sensor variations,and insufficient computational efficiency.To address these challenges,this paper proposes a novel Multi-scale Efficient Wavelet-based Change Detection Network(MewCDNet),which integrates the advantages of Convolutional Neural Networks and Transformers,balances computational costs,and achieves high-performance building change detection.The network employs EfficientNet-B4 as the backbone for hierarchical feature extraction,integrates multi-level feature maps through a multi-scale fusion strategy,and incorporates two key modules:Cross-temporal Difference Detection(CTDD)and Cross-scale Wavelet Refinement(CSWR).CTDD adopts a dual-branch architecture that combines pixel-wise differencing with semanticaware Euclidean distance weighting to enhance the distinction between true changes and background noise.CSWR integrates Haar-based Discrete Wavelet Transform with multi-head cross-attention mechanisms,enabling cross-scale feature fusion while significantly improving edge localization and suppressing spurious changes.Extensive experiments on four benchmark datasets demonstrate MewCDNet’s superiority over comparison methods:achieving F1 scores of 91.54%on LEVIR,93.70%on WHUCD,and 64.96%on S2Looking for building change detection.Furthermore,MewCDNet exhibits optimal performance on the multi-class⋅SYSU dataset(F1:82.71%),highlighting its exceptional generalization capability. 展开更多
关键词 Remote sensing change detection deep learning wavelet transform MULTI-SCALE
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Research Progress on Spatiotemporal Variability of Rice Planting Based on Satellite Remote Sensing Monitoring
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作者 Qi ang HU Aichuan LI +2 位作者 Xinbing WANG Francesco Marinello Zhan SHI 《Agricultural Biotechnology》 2026年第1期76-81,共6页
As a vital food crop,rice is an important part of global food crops.Studying the spatiotemporal changes in rice cultivation facilitates early prediction of production risks and provides support for agricultural policy... As a vital food crop,rice is an important part of global food crops.Studying the spatiotemporal changes in rice cultivation facilitates early prediction of production risks and provides support for agricultural policy decisions related to rice.With the increasing application of satellite remote sensing technology in crop monitoring,remote sensing for rice cultivation has emerged as a novel approach,offering new perspectives for monitoring rice planting.This paper briefly outlined the current research and development status of satellite remote sensing for monitoring rice cultivation both at home and abroad.Foreign scholars have made innovations in data sources and methodologies for satellite remote sensing monitoring,and utilized multi-source satellite information and machine learning algorithms to enhance the accuracy of rice planting monitoring.Scholars in China have achieved significant results in the study of satellite remote sensing for monitoring rice cultivation.Their research and application in monitoring rice planting areas provide valuable references for agricultural production management.However,satellite remote sensing monitoring of rice still faces challenges such as low spatiotemporal resolution and difficulties related to cloud cover and data fusion,which require further in-depth investigation.Additionally,there are shortcomings in the accuracy of remote sensing monitoring for fragmented farmland plots and smallholder farming.To address these issues,future efforts should focus on developing multi-source heterogeneous data fusion analysis technologies and researching monitoring systems.These advancements are expected to enable high-precision large-scale acquisition of rice planting information,laying a foundation for future smart agriculture. 展开更多
关键词 Satellite remote sensing Rice cultivation Spatiotemporal variability MONITORING Research review
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Enhanced Multi-Scale Feature Extraction Lightweight Network for Remote Sensing Object Detection
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作者 Xiang Luo Yuxuan Peng +2 位作者 Renghong Xie Peng Li Yuwen Qian 《Computers, Materials & Continua》 2026年第3期2097-2118,共22页
Deep learning has made significant progress in the field of oriented object detection for remote sensing images.However,existing methods still face challenges when dealing with difficult tasks such as multi-scale targ... Deep learning has made significant progress in the field of oriented object detection for remote sensing images.However,existing methods still face challenges when dealing with difficult tasks such as multi-scale targets,complex backgrounds,and small objects in remote sensing.Maintaining model lightweight to address resource constraints in remote sensing scenarios while improving task completion for remote sensing tasks remains a research hotspot.Therefore,we propose an enhanced multi-scale feature extraction lightweight network EM-YOLO based on the YOLOv8s architecture,specifically optimized for the characteristics of large target scale variations,diverse orientations,and numerous small objects in remote sensing images.Our innovations lie in two main aspects:First,a dynamic snake convolution(DSC)is introduced into the backbone network to enhance the model’s feature extraction capability for oriented targets.Second,an innovative focusing-diffusion module is designed in the feature fusion neck to effectively integrate multi-scale feature information.Finally,we introduce Layer-Adaptive Sparsity for magnitude-based Pruning(LASP)method to perform lightweight network pruning to better complete tasks in resource-constrained scenarios.Experimental results on the lightweight platform Orin demonstrate that the proposed method significantly outperforms the original YOLOv8s model in oriented remote sensing object detection tasks,and achieves comparable or superior performance to state-of-the-art methods on three authoritative remote sensing datasets(DOTA v1.0,DOTA v1.5,and HRSC2016). 展开更多
关键词 Deep learning object detection feature extraction feature fusion remote sensing
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Strain localization and time-dependent deformation in granodiorite characterized by distributed optical fiber sensing
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作者 Shuting Miao Arno Zang +3 位作者 Guido Blöcher Yinlin Ji Hannes Hofmann Pengzhi Pan 《Journal of Rock Mechanics and Geotechnical Engineering》 2026年第1期166-178,共13页
A multi-stage stress relaxation test was performed on a granodiorite sample to understand the deformation process prior to the macroscopic failure of brittle rocks,as well as the transient response during stress relax... A multi-stage stress relaxation test was performed on a granodiorite sample to understand the deformation process prior to the macroscopic failure of brittle rocks,as well as the transient response during stress relaxation.Distributed optical fiber sensing was used to measure strains across the sample surface by helically wrapping the single-mode fiber around the cylindrical sample.Close agreement was observed between the circumferential strains obtained from the optical fibers and the extensometer.The reconstructed full-field strain contours show strain heterogeneity from the crack closure phase,and the strains in the later deformation phase are dominantly localized within the former high-strain zone.The Gini coefficient was used to quantify the degree of strain localization and shows an initial increase during the crack closure phase,a decrease during the linear elastic phase,and a subsequent increase during the post-yielding phase.This behavior corresponds to a process of initial localization from an imperfect boundary condition,homogenization,and eventual relocalization prior to the macroscopic failure of the sample.The transient strain rate decay during the stress relaxation phase was quantified using the p-value in the“Omori-like"power law function.A higher initial stress at the onset of relaxation results in a lower p-value,indicating a slower strain rate decay.As the sample approaches macroscopic failure,the lowest p-value shifts from the most damaged zone to adjacent areas,suggesting stress redistribution or crack propagation in deformed crystalline rocks under stress relaxation conditions. 展开更多
关键词 Distributed optical fiber sensing Stress relaxation Strain localization Time-dependent deformation
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YOLO-DS:a detection model for desert shrub identification and coverage estimation in UAV remote sensing
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作者 Weifan Xu Huifang Zhang +6 位作者 Yan Zhang Kangshuo Liu Jinglu Zhang Yali Zhu Baoerhan Dilixiati Jifeng Ning Jian Gao 《Journal of Forestry Research》 2026年第1期242-255,共14页
Desert shrubs are indispensable in maintaining ecological stability by reducing soil erosion,enhancing water retention,and boosting soil fertility,which are critical factors in mitigating desertification processes.Due... Desert shrubs are indispensable in maintaining ecological stability by reducing soil erosion,enhancing water retention,and boosting soil fertility,which are critical factors in mitigating desertification processes.Due to the complex topography,variable climate,and challenges in field surveys in desert regions,this paper proposes YOLO-Desert-Shrub(YOLO-DS),a detection method for identifying desert shrubs in UAV remote sensing images based on an enhanced YOLOv8n framework.This method accurately identifying shrub species,locations,and coverage.To address the issue of small individual plants dominating the dataset,the SPDconv convolution module is introduced in the Backbone and Neck layers of the YOLOv8n model,replacing conventional convolutions.This structural optimization mitigates information degradation in fine-grained data while strengthening discriminative feature capture across spatial scales within desert shrub datasets.Furthermore,a structured state-space model is integrated into the main network,and the MambaLayer is designed to dynamically extract and refine shrub-specific features from remote sensing images,effectively filtering out background noise and irrelevant interference to enhance feature representation.Benchmark evaluations reveal the YOLO-DS framework attains 79.56%mAP40weight,demonstrating 2.2%absolute gain versus the baseline YOLOv8n architecture,with statistically significant advantages over contemporary detectors in cross-validation trials.The predicted plant coverage exhibits strong consistency with manually measured coverage,with a coefficient of determination(R^(2))of 0.9148 and a Root Mean Square Error(RMSE)of1.8266%.The proposed UAV-based remote sensing method utilizing the YOLO-DS effectively identify and locate desert shrubs,monitor canopy sizes and distribution,and provide technical support for automated desert shrub monitoring. 展开更多
关键词 Desert shrubs Deep learning Object detection UAV remote sensing YOLOv8 Mamba
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Photochromic poly(lactic acid)/poly(3-hydroxybutyrate)/thermoplastic polyurethane/carbon black nanoparticle composite strain-sensing yarn with coaxial hollow-core-sheath structure for multifunctional textiles
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作者 Tao YAN Yehan ZHU +2 位作者 Yuting WU Xin ZHANG Zhijuan PAN 《Science China(Technological Sciences)》 2026年第3期86-100,共15页
The development of multifunctional intelligent textiles has become an important innovation direction in the field of textile engineering, under the dual demands of intelligent health monitoring and environmental prote... The development of multifunctional intelligent textiles has become an important innovation direction in the field of textile engineering, under the dual demands of intelligent health monitoring and environmental protection. Although singlefunctional textiles with antibacterial, photochromic, and strain-sensing properties have been developed, they are unable to meet the demand for multifunctional textiles. In this respect, this study developed a poly(lactic acid) and poly(3-hydroxybutyrate)/thermoplastic polyurethane/carbon black nanoparticle composite nanofiber yarn(PPTCY) with a hollow-core-sheath structure using a simple conjugate electrospinning technology. PPTCY possessed excellent mechanical strength and could be effectively woven. More importantly, it not only enabled real-time visual monitoring of ultraviolet(UV) intensity in the environment but also possessed excellent antibacterial properties and strain-sensing performance. Its ΔE value was up to 58.24, and its antibacterial rates against Escherichia coli and Staphylococcus aureus were both 99.99%. This fabric had excellent strainsensing performance, high linearity, and durability under both pressure and stretching deformations. This research provides favorable technical support for the application of intelligent textiles in the field of UV protection and traffic safety. 展开更多
关键词 conjugated electrospinning hollow-core-sheath structure photochromic antibacterial yarn dual-mode strain sensing multifunctional textiles
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Key roles of Young’s modulus and mechanical hysteresis in hydrogel strain sensors for high-fidelity sensing
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作者 Yuanlai Fang Jialin Li +5 位作者 Zhongxiang Bai Jingjiang Wei Kun Yang Li Yang Qingyuan Wang Jiaxi Cui 《Science China Materials》 2026年第3期1624-1633,共10页
Conductive hydrogel-based stretchable electronics have been extensively investigated,among which strain sensors are the most prominently studied.While the mechanical properties significantly affect the performance of ... Conductive hydrogel-based stretchable electronics have been extensively investigated,among which strain sensors are the most prominently studied.While the mechanical properties significantly affect the performance of these devices,the systematic correlation between specific mechanical parameters and sensing performance remains rarely explored.This work compares the influences of Young’s modulus and mechanical hysteresis on the sensing performance between highly entangled PAM-Li and double-network PAM-Li-Agar-3 strain sensors.Owing to the brittle agar network,which imparts a higher Young’s modulus and pronounced mechanical hysteresis to the double-network PAMLi-Agar-3 hydrogel,the corresponding sensor requires a greater driving force for deformation and yields signals with poor reproducibility.In comparison,the PAM-Li hydrogel,characterized by highly entangled polymer chains,exhibits a lower Young’s modulus and negligible mechanical hysteresis.Consequently,signals from the PAM-Li strain sensor demonstrate enhanced sensitivity and stability.Therefore,this work demonstrates that a low Young’s modulus and minimal mechanical hysteresis are critical factors for achieving superior sensing performance in strain sensors,as systematically validated through comparative analyses across diverse application scenarios. 展开更多
关键词 conductive hydrogel strain sensor Young’s modulus mechanical hysteresis sensing quality
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An Overview of Remote Sensing of Agricultural Greenhouses:Advances and Perspectives
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作者 GAO Yuan ZHU Bingxue SONG Kaishan 《Chinese Geographical Science》 2026年第2期171-190,共20页
Agricultural greenhouses(AGHs)are increasingly used globally to control the crop growth environment,which are vital for food production,resource conservation,and rural economies.Advances in high-quality data acquisiti... Agricultural greenhouses(AGHs)are increasingly used globally to control the crop growth environment,which are vital for food production,resource conservation,and rural economies.Advances in high-quality data acquisition methods and information retrieval algorithms have improved the ability to extract AGHs from remote sensing images(e.g.,satellite and uncrewed aerial vehicle(UAV)).Research on this topic began in 1989,and the number of related studies has increased annually.This paper provides a review of the development of remote sensing of AGHs and research hotspots.It summarizes the current status and trends of data sources,identification features,methods,and accuracy of AGHs extraction.Due to the unique spectral,textural,and geometric characteristics of AGHs,research studies have primarily utilized optical remote sensing data from sensors with spatial resolutions of 30 m or more,such as Landsat,Sentinel,Gaofen(GF),and Worldview,to extract AGHs.Machine learning and deep learning methods have provided more precise results for extracting AGHs than threshold segmentation methods.In contrast,deep learning algorithms have been primarily used with high-spatial resolution data and small-scale study areas,with accuracy rates generally exceeding 90.00%.However,future research may use higher spatial resolution images to improve the accuracy and detail of AGH extraction.Recent studies have integrated multiple data sources and performed time-series analysis to improve monitoring of dynamic changes in AGHs.Moreover,emphasis should be placed on optimizing data fusion techniques,implementing sample transfer methods,expanding the number of sensors,and increasing the application of artificial intelligence(AI)in monitoring AGHs.These efforts will provide more reliable methods and tools to improve agricultural production and resource utilization efficiency.This review provides resources for researchers and decision-makers involved in modern agricultural development,as well as scientific evidence for the sustainable development of rural areas. 展开更多
关键词 agricultural greenhouse(AGH) remote sensing deep learning precision agriculture time-series analysis
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Deep Learning-Enhanced Human Sensing with Channel State Information: A Survey
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作者 Binglei Yue Aili Jiang +3 位作者 Chun Yang Junwei Lei Heng Liu Yin Zhang 《Computers, Materials & Continua》 2026年第1期1-28,共28页
With the growing advancement of wireless communication technologies,WiFi-based human sensing has gained increasing attention as a non-intrusive and device-free solution.Among the available signal types,Channel State I... With the growing advancement of wireless communication technologies,WiFi-based human sensing has gained increasing attention as a non-intrusive and device-free solution.Among the available signal types,Channel State Information(CSI)offers fine-grained temporal,frequency,and spatial insights into multipath propagation,making it a crucial data source for human-centric sensing.Recently,the integration of deep learning has significantly improved the robustness and automation of feature extraction from CSI in complex environments.This paper provides a comprehensive review of deep learning-enhanced human sensing based on CSI.We first outline mainstream CSI acquisition tools and their hardware specifications,then provide a detailed discussion of preprocessing methods such as denoising,time–frequency transformation,data segmentation,and augmentation.Subsequently,we categorize deep learning approaches according to sensing tasks—namely detection,localization,and recognition—and highlight representative models across application scenarios.Finally,we examine key challenges including domain generalization,multi-user interference,and limited data availability,and we propose future research directions involving lightweight model deployment,multimodal data fusion,and semantic-level sensing. 展开更多
关键词 Channel State Information(CSI) human sensing human activity recognition deep learning
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Quorum sensing:its roles in mediating biofilm and viable but non-culturable state formation,and strategies for the prevention and control of foodborne bacteria via quorum quenching
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作者 Ting Ding Xuchen Li +3 位作者 Hongwei Zhan Yanqing Li Zhenqing Li Yang Deng 《Food Science and Human Wellness》 2026年第2期520-537,共18页
Foodborne bacteria produce biofilms and their viable but non-culturable(VBNC)formation,can affect food quality and safety.Studies have shown that these characteristics are regulated by the bacterial quorum sensing(QS)... Foodborne bacteria produce biofilms and their viable but non-culturable(VBNC)formation,can affect food quality and safety.Studies have shown that these characteristics are regulated by the bacterial quorum sensing(QS)system.Quenching the QS system of foodborne bacteria and blocking the expression of the corresponding genes may be an effective way to improve food quality and safety.Therefore,this article reviews the QS systems for foodborne bacteria,the regulatory mechanisms of QS systems in biofilm and VBNC formation and resuscitation,the research progress on quorum sensing inhibitors(QSIs)for Gram-negative and Gram-positive bacteria,and introduces QSIs from various sources.In addition,we have also summarized the current research issues on QS regulation of biofilms and VBNC formation.The systematic study of the QS phenomenon of foodborne bacteria in practical situations,the mechanism of bacterial QS cooperation-cheating,the screening of novel and highly active QSIs,the combination of QSIs and other technologies to improve their bioavailability,and the regulatory network between biofilm and VBNC formation and resuscitation are research directions that need to be paid attention to in the future. 展开更多
关键词 Foodborne bacteria Quorum sensing Biofilm formation Viable but non-culturable state formation Food quality
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Statistical method for quantifying the strain localization process in Beishan granite under multi-creep triaxial compression based on distributed optical fiber sensing
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作者 Xiujun Zhang Peng-Zhi Pan Shuting Miao 《Journal of Rock Mechanics and Geotechnical Engineering》 2026年第1期398-415,共18页
To investigate the damage evolution caused by stress-driven and sub-critical crack propagation within the Beishan granite under multi-creep triaxial compressive conditions,the distributed optical fiber sensing and X-r... To investigate the damage evolution caused by stress-driven and sub-critical crack propagation within the Beishan granite under multi-creep triaxial compressive conditions,the distributed optical fiber sensing and X-ray computed tomography were combined to obtain the strain distribution over the sample surface and internal fractures of the samples.The Gini and skewness(G-S)coefficients were used to quantify strain localization during tests,where the Gini coefficient reflects the degree of clustering of elements with high strain values,i.e.,strain localization/delocalization.The strain localization-induced asymmetry of data distribution is quantified by the skewness coefficient.A precursor to granite failure is defined by the rapid and simultaneous increase of the G-S coefficients,which are calculated from strain increment,giving an earlier warning of failure by about 8%peak stress than those from absolute strain values.Moreover,the process of damage accumulation due to stress-driven crack propagation in Beishan granite is different at various confining pressures as the stress exceeds the crack initiation stress.Concretely,strain localization is continuous until brittle failure at higher confining pressure,while both strain localization and delocalization occur at lower confining pressure.Despite the different stress conditions,a similar statistical characteristic of strain localization during the creep stage is observed.The Gini coefficient increases,and the skewness coefficient decreases slightly as the creep stress is below 95%peak stress.When the accelerated strain localization begins,the Gini and skewness coefficients increase rapidly and simultaneously. 展开更多
关键词 Statistical method Multi-creep triaxial compression Strain localization quantification Distributed optical fiber sensing Precursor identification
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