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Fosl-1对肾母细胞瘤动物模型中Wnt通路及肿瘤转移相关因子的影响
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作者 海力其古丽·努日丁 古丽巴哈·买买提 +2 位作者 刘玉 王学梅 严媚 《新疆医科大学学报》 2026年第2期198-204,共7页
目的探讨Fosl-1、Wnt通路及肿瘤转移相关因子与肾母细胞瘤肿瘤转移的相关性,为寻找肾母细胞瘤治疗靶点提供理论依据。方法将慢病毒Fosl-1干扰的WIT49肾母细胞瘤细胞及Fosl-1干扰空载的WIT49细胞分别皮下接种于BALB/c-Nude雄性裸鼠以构... 目的探讨Fosl-1、Wnt通路及肿瘤转移相关因子与肾母细胞瘤肿瘤转移的相关性,为寻找肾母细胞瘤治疗靶点提供理论依据。方法将慢病毒Fosl-1干扰的WIT49肾母细胞瘤细胞及Fosl-1干扰空载的WIT49细胞分别皮下接种于BALB/c-Nude雄性裸鼠以构建移植瘤模型。实验动物分为4组:对照组(皮下接种未处理的WIT49细胞)、Fosl-1干扰-NC组(皮下接种空载慢病毒转染的WIT49细胞)、Fosl-1干扰组(皮下接种Fosl-1干扰慢病毒转染的WIT49细胞)和Fosl-1干扰+Wnt抑制组(皮下接种Fosl-1干扰慢病毒转染的WIT49细胞,在接种后进行腹腔注射Wnt抑制剂FH535)。观察肿瘤生长并测量瘤体体积;采用实时荧光定量逆转录聚合酶链式反应(qRT-PCR)检测Fosl-1 mRNA表达;通过蛋白质免疫印迹(Western blot)检测Wnt通路关键蛋白[卷曲蛋白1(FZD1)、Wnt家族成员1(Wnt-1)、β-连环蛋白(β-catenin)]、肿瘤转移相关蛋白[基质金属蛋白酶9(MMP-9)、肿瘤转移抑制蛋白23(NM23)]及促凋亡蛋白Bax的表达;运用HE染色及免疫组织化学法观察肿瘤组织形态并检测Fosl-1蛋白表达。结果与对照组相比,Fosl-1干扰组和Fosl-1干扰+Wnt抑制组裸鼠肿瘤体积在第13天和第16天均减小(P<0.05)。在接种细胞后第10天、第13天和第16天,与Fosl-1干扰组相比,Fosl-1干扰+Wnt抑制组裸鼠的肿瘤体积均更小,差异有统计学意义(P<0.05)。与对照组相比,Fosl-1干扰组和Fosl-1干扰+Wnt抑制组的Fosl-1 mRNA、Wnt信号通路相关关键蛋白FZD1、Wnt-1、β-catenin及肿瘤转移相关蛋白MMP-9、NM23的表达均下降,而促凋亡蛋白Bax的表达升高(P<0.05)。HE染色结果显示,与对照组相比,Fosl-1干扰组及Fosl-1干扰+Wnt抑制组肿瘤细胞核分裂像减少,核浆比降低,间质结构更疏松,同时,空泡状细胞结构增加,尤其Fosl-1干扰+Wnt抑制组空泡状细胞结构增加更明显。免疫组化检测结果显示,与对照组相比,Fosl-1干扰组和Fosl-1干扰+Wnt抑制组中Fosl-1蛋白的表达量均降低,差异有统计学意义(P<0.05)。结论抑制Fosl-1能有效延缓肾母细胞瘤的生长并降低肿瘤转移相关因子的表达。联合抑制Wnt通路能进一步增强对肿瘤生长的抑制作用,提示Fosl-1在肾母细胞瘤中的作用可能依赖于Wnt/β-catenin信号通路。 展开更多
关键词 fosl-1 肾母细胞瘤 WNT通路 肿瘤转移
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DUSP10调控c-Fos/NFATc1信号通路抑制破骨细胞分化研究
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作者 刘梦想 陈磊 于洪涛 《中国医学工程》 2026年第2期10-14,共5页
目的探讨DUSP10对破骨细胞分化的调控作用及其分子机制。方法使用RAW264.7细胞作为模型,将细胞分为NC组(未转染质粒,正常培养细胞),DUSP10-NC组(转染DUSP10空载质粒),DUSP10-OE组(转染DUSP10过表达质粒),DUSP10-sh组(转染DUSP10干扰质... 目的探讨DUSP10对破骨细胞分化的调控作用及其分子机制。方法使用RAW264.7细胞作为模型,将细胞分为NC组(未转染质粒,正常培养细胞),DUSP10-NC组(转染DUSP10空载质粒),DUSP10-OE组(转染DUSP10过表达质粒),DUSP10-sh组(转染DUSP10干扰质粒),通过CCK-8法检测细胞活性,TRAP染色评估破骨细胞分化,Western blot检测c-Fos、NFATc1及TRAP蛋白表达水平。结果DUSP10过表达抑制破骨细胞活性(P<0.05),并减少TRAP阳性细胞数量(P<0.05),同时降低c-Fos、NFATc1和TRAP蛋白表达水平(P<0.05);而抑制DUSP10则促进破骨细胞分化(P<0.005),并上调上述蛋白表达。结论DUSP10可通过抑制c-Fos/NFATc1信号通路降低破骨细胞分化活性,为骨质疏松症等骨代谢疾病的靶向治疗提供了新的理论依据。 展开更多
关键词 DUSP10 破骨细胞 C-fos NFATc1 TRAP
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lncRNA XIST调控miR-17-5p/FOSL1轴对胆囊癌细胞增殖、迁移和侵袭的影响
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作者 陈李康 姚磊 彭莹莹 《肝胆胰外科杂志》 2026年第2期115-123,共9页
目的探讨长链非编码RNA(lncRNA)X染色体失活特异性转录本(XIST)调控微小RNA-17-5p(miR-17-5p)/FOS样抗原1(FOSL1)轴对胆囊癌(GBC)细胞增殖、迁移和侵袭的影响。方法qRT-PCR法检测GBC组织和细胞株中mRNA表达;双荧光素酶报告基因实验验证l... 目的探讨长链非编码RNA(lncRNA)X染色体失活特异性转录本(XIST)调控微小RNA-17-5p(miR-17-5p)/FOS样抗原1(FOSL1)轴对胆囊癌(GBC)细胞增殖、迁移和侵袭的影响。方法qRT-PCR法检测GBC组织和细胞株中mRNA表达;双荧光素酶报告基因实验验证lncRNA XIST与miR-17-5p,miR-17-5p与FOSL1的互作;将EH-GB1细胞分为Ctrl组、sh-NC组、sh-XIST组、sh-XIST+inhibitor-NC组、sh-XIST+inhibitor-miR-17-5p组、mimic-NC组、mimic-miR-17-5p组、mimic-miR-17-5p+OE-NC组、mimic-miR-17-5p+OE-FOSL1组。台盼蓝染色和平板克隆实验检测EH-GB1细胞增殖;划痕试验检测EH-GB1细胞迁移;Transwell实验检测EH-GB1细胞侵袭;Western blotting检测EH-GB1细胞中Ki67、Cyclin D1、MMP-2、CD44、FOSL1蛋白的表达。裸鼠移植瘤实验检测敲低lncRNA XIST对GBC肿瘤生长的影响。结果GBC组织和细胞中miR-17-5p呈低表达,lncRNA XIST、FOSL1呈高表达。sh-XIST组细胞存活率、增殖数、划痕愈合率、侵袭数,Ki67、Cyclin D1、MMP-2、CD44表达水平低于sh-NC组、Ctrl组(P<0.05);sh-XIST+inhibitor-miR-17-5p组细胞存活率、增殖数、划痕愈合率、侵袭数及Ki67、Cyclin D1、MMP-2、CD44表达水平高于sh-XIST组、sh-XIST+inhibitor-NC组(P<0.05)。mimic-miR-17-5p组细胞存活率、增殖数、划痕愈合率、侵袭数及Ki67、Cyclin D1、MMP-2、CD44、FOSL1表达水平低于mimic-NC组、Ctrl组(P<0.05);mimic-miR-17-5p+OE-FOSL1组细胞存活率、增殖数、划痕愈合率、侵袭数及Ki67、Cyclin D1、MMP-2、CD44、FOSL1表达水平高于mimic-miR-17-5p组、mimic-miR-17-5p+OE-NC组(P<0.05)。lncRNA XIST可以靶向负调控miR-17-5p,而miR-17-5p可以靶向负调控FOSL1(P<0.05)。XIST敲低组裸鼠移植瘤体积、体质量,肿瘤FOSL1蛋白、lncRNA XIST表达水平低于阴性对照组,miR-17-5p表达水平高于阴性对照组(P<0.05)。结论lncRNA XIST通过调控miR-17-5p/FOSL1轴促进GBC细胞的增殖、迁移和侵袭。 展开更多
关键词 长链非编码RNA X染色体失活特异性转录本 微小RNA-17-5p fos样抗原1 胆囊癌 增殖 迁移 侵袭
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和胃安神方对失眠模型大鼠视交叉上核c-fos、c-jun及神经元型一氧化氮合酶表达的影响
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作者 刘子涵 王硕 +7 位作者 邹涛 田楚娇 于泓洋 底昂 王亮 陈琦 王长振 李绍旦 《环球中医药》 2026年第1期36-44,共9页
目的探讨和胃安神方对失眠模型大鼠视交叉上核(suprachiasmatic nucleus,SCN)即刻早期基因c-fos、c-jun以及神经元型一氧化氮合酶(neuronal nitric oxide synthase,nNOS)表达的影响。方法选用60只雄性SD大鼠,随机分配至正常对照组、模... 目的探讨和胃安神方对失眠模型大鼠视交叉上核(suprachiasmatic nucleus,SCN)即刻早期基因c-fos、c-jun以及神经元型一氧化氮合酶(neuronal nitric oxide synthase,nNOS)表达的影响。方法选用60只雄性SD大鼠,随机分配至正常对照组、模型对照组、艾司唑仑组以及和胃安神方低、中、高剂量组,每组10只。除正常对照组外,其余各组大鼠均通过腹腔注射对氯苯丙氨酸诱导失眠模型。造模效果通过戊巴比妥钠翻正实验进行评估。造模成功后,各组大鼠接受连续7天的灌胃给药:正常对照组与模型对照组灌服10 mL/kg蒸馏水;艾司唑仑组给予0.33 mg/(kg·d)艾司唑仑水溶液;和胃安神方低、中、高剂量组则分别灌胃浓度为1 g/mL、2 g/mL、4 g/mL的和胃安神方药液,给药体积均为10 mL/kg。给药7天完成后,开展旷场实验及高架十字迷宫实验以评估大鼠行为并采用免疫组化法和qPCR法检测大鼠SCN中c-fos、c-jun以及nNOS表达情况。结果造模结束后,与正常对照组相比,造模组大鼠睡眠潜伏期延长(P<0.01),睡眠时间缩短(P<0.01)。末次给药后,与正常对照组相比,模型对照组大鼠旷场运动总路程、平均速度显著增加(P<0.01),旷场中央区停留时间显著下降(P<0.01),高架十字迷宫开放臂进入次数比例、开放臂停留时间比例下降(P<0.01),SCN中c-fos、c-jun及nNOS的表达水平显著升高(P<0.01)。与模型对照组相比,艾司唑仑组与和胃安神方中、高剂量组大鼠旷场运动总路程、平均速度显著下降(P<0.05或P<0.01),旷场中央区停留时间显著上升(P<0.05或P<0.01);艾司唑仑组与和胃安神方低、中、高剂量组大鼠高架十字迷宫进入开放臂次数比例、开放臂停留时间比例均升高(P<0.05或P<0.01),SCN中c-fos、c-jun及nNOS的表达水平显著降低(P<0.05或P<0.01)。结论和胃安神方能有效缓解失眠模型大鼠的睡眠障碍,可能与其对SCN中c-fos、c-jun及nNOS表达水平的下调作用相关。 展开更多
关键词 失眠 和胃安神方 视交叉上核 c-fos c-jun 神经元型一氧化氮合酶
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c-Fos蛋白在眼科疾病中的作用研究进展
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作者 尚孟秋(综述) 梁丽娜(审校) 《安徽医科大学学报》 北大核心 2026年第2期362-368,共7页
c-Fos蛋白作为细胞活性及神经环路的功能解剖学标志物,在神经内分泌调节、自主神经活动及应激等刺激诱发的行为反应研究中具有广泛应用价值。近年来研究显示,在眼科领域,c-Fos的动态表达与视网膜神经节细胞凋亡、视皮质可塑性、光损伤... c-Fos蛋白作为细胞活性及神经环路的功能解剖学标志物,在神经内分泌调节、自主神经活动及应激等刺激诱发的行为反应研究中具有广泛应用价值。近年来研究显示,在眼科领域,c-Fos的动态表达与视网膜神经节细胞凋亡、视皮质可塑性、光损伤修复及血管生成等病理生理过程密切相关。本文旨在对c-Fos蛋白在青光眼、弱视、视网膜病变等眼部疾病中的作用机制进行综述;同时探讨通过调控c-Fos蛋白针对这些疾病的潜在治疗方法。本研究表明,激活c-Fos蛋白可以促进视皮质神经元的发育,在视网膜视神经疾病中,抑制c-Fos蛋白表达能够延缓视网膜神经节细胞、神经胶质细胞、光感受器细胞等细胞的凋亡。 展开更多
关键词 C-fos蛋白 眼科疾病 青光眼 弱视 视网膜色素变性 糖尿病视网膜病变
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Noninvasive Radar Sensing Augmented with Machine Learning for Reliable Detection of Motor Imbalance
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作者 Faten S.Alamri Adil Ali Saleem +2 位作者 Muhammad I.Khan Hafeez Ur Rehman Siddiqui Amjad Rehman 《Computer Modeling in Engineering & Sciences》 2026年第1期698-726,共29页
Motor imbalance is a critical failure mode in rotating machinery,potentially causing severe equipment damage if undetected.Traditional vibration-based diagnostic methods rely on direct sensor contact,leading to instal... Motor imbalance is a critical failure mode in rotating machinery,potentially causing severe equipment damage if undetected.Traditional vibration-based diagnostic methods rely on direct sensor contact,leading to installation challenges and measurement artifacts that can compromise accuracy.This study presents a novel radar-based framework for non-contact motor imbalance detection using 24 GHz continuous-wave radar.A dataset of 1802 experimental trials was sourced,covering four imbalance levels(0,10,20,30 g)across varying motor speeds(500–1500 rpm)and load torques(0–3 Nm).Dual-channel in-phase and quadrature radar signals were captured at 10,000 samples per second for 30-s intervals,preserving both amplitude and phase information for analysis.A multi-domain feature extraction methodology captured imbalance signatures in time,frequency,and complex signal domains.From 65 initial features,statistical analysis using Kruskal–Wallis tests identified significant descriptors,and recursive feature elimination with Random Forest reduced the feature set to 20 dimensions,achieving 69%dimensionality reduction without loss of performance.Six machine learning algorithms,Random Forest,Extra Trees Classifier,Extreme Gradient Boosting,Categorical Boosting,Support Vector Machine with radial basis function kernel,and k-Nearest Neighbors were evaluated with grid-search hyperparameter optimization and five-fold cross-validation.The Extra Trees Classifier achieved the best performance with 98.52%test accuracy,98%cross-validation accuracy,and minimal variance,maintaining per-class precision and recall above 97%.Its superior performance is attributed to its randomized split selection and full bootstrapping strategy,which reduce variance and overfitting while effectively capturing the nonlinear feature interactions and non-normal distributions present in the dataset.The model’s average inference time of 70 ms enables near real-time deployment.Comparative analysis demonstrates that the radar-based framework matches or exceeds traditional contact-based methods while eliminating their inherent limitations,providing a robust,scalable,and noninvasive solution for industrial motor condition monitoring,particularly in hazardous or space-constrained environments. 展开更多
关键词 Condition monitoring imbalance detection industrial applications machine learning motor fault diagnosis non-contact sensing radar sensing vibration monitoring
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Azobenzene-winged phenanthroline for supramolecular chirality sensing and multidimensional chiroptical manipulation via solvent,light,temperature,and redox
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作者 Xiaoqian Wang Yanling Shen +6 位作者 Long Chen Lizhi Fang Kuppusamy Kanagaraj Ming Rao Chunying Fan Wanhua Wu Cheng Yang 《Chinese Chemical Letters》 2026年第2期453-457,共5页
Azobenzene-winged phenanthrolines(L1 and L2)were designed,synthesized,and fully characterized.Ligand L1 forms an in-situ cobalt complex,which has been effectively employed as a circular dichroism(CD)-active chiral sen... Azobenzene-winged phenanthrolines(L1 and L2)were designed,synthesized,and fully characterized.Ligand L1 forms an in-situ cobalt complex,which has been effectively employed as a circular dichroism(CD)-active chiral sensor.The resulting ternary complex(L1-Co^(2+)-amino alcohol)exhibits pronounced exciton-coupled circular dichroism(ECCD)signals at the characteristic azobenzene absorption bands.These signals arise from efficient chirality transfer from the chiral amino alcohol to the azobenzene chromophores,enabling the determination of the absolute configuration of chiral amino alcohols.Accordingly,the L1-Co^(2+)coordination system demonstrates considerably potential in chirality sensing applications.Remarkably,the induced ECCD signals are highly responsive to multiple external stimuli,including photoirradiation,solvent polarity,temperature,and redox conditions.In particular,temperature and redox changes can induce a reversible inversion of the ECCD signal,thereby establishing this system as a multifunctional,stimuli-responsive chiroptical molecular switch. 展开更多
关键词 Phenanthroline derivative AZOBENZENE Amino alcohols Chirality sensing Stimuli-response
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Entropy-Driven Cellulosic Elastomer Self-Assembly for Mechanical Energy Harvesting and Self-Powered Sensing
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作者 Pinle Zhang Yingping He +5 位作者 Huancheng Huang Neng Xiong Xinyue Nong Xinke Yu Shuangfei Wang Xinliang Liu 《Nano-Micro Letters》 2026年第6期898-941,共44页
The rapid advancement of flexible electronics technology has placed higher demands on the structural design and performance regulation of elastic materials.Cellulosic elastomers,with their biodegradability,renewabilit... The rapid advancement of flexible electronics technology has placed higher demands on the structural design and performance regulation of elastic materials.Cellulosic elastomers,with their biodegradability,renewability,and tunability,emerge as ideal candidate materials.Entropy-driven self-as sembly promotes the spontaneous formation of ordered structures,serving as a crucial pathway for optimizing cellulose elastomer properties.However,the structure-property relationship between the self-assembled ordered structures of cellulose elastomers and their mechanical and electrical properties remains insufficiently explored.It hinders the expansion of their applications in electronic devices.This paper systematically reviews the structure-property regulation mechanisms of self-assembled cellulosic elastomers from an entropy-driven perspective.It elucidates the application principles and performance optimization strategies for mechanical energy harvesting and self-powered sensing,while also exploring the challenges and prospects for performance enhancement.This work provides a reference for the development of self-assembled cellulosic elastomers in the field of energy devices. 展开更多
关键词 Cellulosic elastomers Entropy-driven self-assembly Mechanoelectric conversion Self-powered sensing
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Strong yet Flexible TiC-SiC Fibrous Membrane with Long-Time Ultrahigh Temperature Resistance for Sensing in Extreme Environment
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作者 Tianyue Yang Yan Shen +5 位作者 Yangzhong Zhao Zhongqian Zhao Xue Zhou Qianji Chen Xujing Wang Yanzi Gou 《Nano-Micro Letters》 2026年第6期16-29,共14页
The demand for sensors capable of operating in extreme environment of the fields,such as aerospace vehicles,aeroengines and fire protection,is rapidly increasing.However,developing flexible ceramic fibrous pressure se... The demand for sensors capable of operating in extreme environment of the fields,such as aerospace vehicles,aeroengines and fire protection,is rapidly increasing.However,developing flexible ceramic fibrous pressure sensors that combine high temperature stability with robust mechanical properties remains a significant challenge.Herein,through precise multi-scale process control,high-strength(2.1 MPa)TiC-SiC flexible fibrous membrane is successfully fabricated.The membrane exhibits exceptional thermal resistance(2000℃)and long–term thermal stability(1800℃ for 5 h)in the inert atmosphere.Meanwhile,the TiC-SiC fibrous membrane shows excellent oxidation resistance and still achieves strength of 1.8 MPa after being oxidized at 1200℃ for 1 h in air.Remarkably,TiC-SiC fibrous membrane withstands a load of approximately 1400 times its own weight and the ablation of butane flame(~1300℃)for at least 1 h without breaking.Notably,after heat treatment at 1800℃ for 5 h in an argon atmosphere,the TiC-SiC fibrous membrane even sustains pressure–sensing performance for up to 300 cycles.The membrane exhibits stable resistivity up to 900℃ and shows sensing stability under butane flame.The results of this work provide an effective and feasible solution to fill the research gap of flexible fibrous sensors for extreme environments. 展开更多
关键词 TiC-SiC Fibrous membrane FLEXIBILITY High temperature stability Pressure sensing
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Enhancing the estimation of soil water content using a resistive heater in the dual-probe distributed temperature sensing approach
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作者 Luis Eduardo Bertotto Alan Reis +1 位作者 Cristina de Hollanda Cavalcanti Tsuha Edson Cezar Wendland 《Journal of Rock Mechanics and Geotechnical Engineering》 2026年第1期625-636,共12页
The dual-probe heat pulse(DPHP)is a well-established method for estimating soil moisture(θ)using soil thermal conductivity(λ)and volumetric heat capacity(C_(v)).Recently,monitoringθhas been improved by integrating ... The dual-probe heat pulse(DPHP)is a well-established method for estimating soil moisture(θ)using soil thermal conductivity(λ)and volumetric heat capacity(C_(v)).Recently,monitoringθhas been improved by integrating the DPHP method with distributed temperature sensing(DTS)technology.In the DPHP-DTS approach,a single fiber optic(FO)cable with embedded metallic constituents functions as a heating element,while a parallel cable serves to monitor the temperature.Despite ongoing advancements,challenges such as the difficulty in positioning heating and sensing cables and high energy requirements hinder the widespread adoption of the DPHP-DTS method.As alternative heating materials are seldom used,this study evaluated the feasibility of employing a resistive metallic alloy as the heating element in a laboratory DPHP-DTS application.Overall,higher errors were observed when assessing C_(v)andλat higherθvalues(>0.2),but using C_(v)data produced more accurateθestimates(with the root mean square error(RMSE)≤0.06).Based on C_(v)values,a low-power,long-duration heat pulse(8.07 W/m for 300 s)yielded more consistentθestimates(RMSE=0.04)than a high-power,shortduration pulse(15.93 W/m for 180 s,with RMSE=0.06).The findings of this study also indicated that variations in heating uniformity and electrical power fluctuations potentially affected measurement accuracy.Nevertheless,the resistive alloy proved advantageous for DPHP-DTS due to its independent power connection,ability to maintain linear positioning within the soil,and potential for energy savings,all while providing reliableθestimates. 展开更多
关键词 Fiber optic(FO) Heat pulse Heating material Soil thermal properties Soil moisture
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Impact of zearalenone on quorum sensing signaling molecules and its association with the suppression of ruminal microbial fermentation in a RUSITEC system
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作者 Zuo Wang Tianyi Ma +6 位作者 Jianhua He Yu Ge Qianglin Liu Xinyi Lan Lei Liu Fachun Wan Weijun Shen 《Journal of Animal Science and Biotechnology》 2026年第2期1119-1134,共16页
Background Zearalenone(ZEN),a common mycotoxin in ruminant diets,could disturb the rumen ecosystem and impair rumen fermentation.Noticeably,ZEN has been shown to reduce the relative abundances of specific bacterial ta... Background Zearalenone(ZEN),a common mycotoxin in ruminant diets,could disturb the rumen ecosystem and impair rumen fermentation.Noticeably,ZEN has been shown to reduce the relative abundances of specific bacterial taxa that potentially possess quorum sensing(QS)functions,which are deemed essential for the microbial interactions and adaptations during rumen fermentation.Nonetheless,whether QS communications participate in the responses of rumen microbial fermentation to ZEN remains unknown.Therefore,the present trial was performed to explore the potential roles of QS during the alterations of rumen microbial fermentation by ZEN through a rumen simulation technique(RUSITEC)system,in a replicated 4×4 Latin square design.Results ZEN significantly(P<0.05)reduced QS signal autoinducer-2(AI-2),and tended to(P=0.051)downregulate QS signal C4-homoserine lactone(HSL).ZEN also significantly(P<0.05)decreased total volatile fatty acid(TVFA),acetate,propionate,isobutyrate,isovalerate,organic matter disappearance(OMD),neutral detergent fiber disappearance(NDFD),and acid detergent fiber disappearance(ADFD)in different manners.The linear discriminant analysis effect size(LEf Se)analysis indicated significantly(P<0.05)differential enrichments of a series of bacterial taxa such as Butyrivibrio_sp_X503,Rhizobium daejeonense,Hoylesella buccalis,Ezakiella coagulans,Enterococcus cecorum,Ruminococcus_sp_zg-924,Polystyrenella longa,and Methylacidimicrobium fagopyrum across different treatments.The phylogenetic investigation of communities by reconstruction of unobserved states 2(PICRUSt2)analysis suggested that QS were predicted to be significantly(P<0.05)affected by ZEN.The metabolomics analysis detected considerable significantly(P<0.05)differing metabolites and implied that ZEN challenge significantly(P<0.05)influenced the indole alkaloid biosynthesis,biosynthesis of alkaloids derived from shikimate pathway,and sesquiterpenoid and triterpenoid biosynthesis.Significant(P<0.05)interconnections of QS molecules with the differential rumen fermentation traits,differential bacterial taxa,and differential metabolites were exhibited by Spearman analysis.Conclusions ZEN negatively affected the QS signals of AI-2 and C4-HSL,which was found to correlate with the fluctuations in specific rumen fermentation characteristics,ruminal bacterial populations,and ruminal metabolisms.These interrelationships implied the potential involvement of QS in the reactions of rumen microbiota to ZEN contamination,and probably contributed to the inhibition of rumen fermentation. 展开更多
关键词 Acyl-homoserine lactones Autoinducer-2 Quorum sensing Rumen microbiome ZEARALENONE
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Integrating optical and radiofrequency interferometry for enhanced phase sensing
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作者 Ruimin Jie Zhaopeng Zhang +1 位作者 Chen Zhu Jie Huang 《Advanced Photonics Nexus》 2026年第1期111-121,共11页
Interferometry is a crucial investigative technique used across diverse fields to achieve highprecision measurements.It works by analyzing the phase difference between two interfering waves,which results from variatio... Interferometry is a crucial investigative technique used across diverse fields to achieve highprecision measurements.It works by analyzing the phase difference between two interfering waves,which results from variations in optical path lengths within an interferometer.We introduce a novel method for directly measuring changes in the phase difference within an optical interferometer,importantly,with the added advantage of a controllable enhancement factor.This approach is achieved through a two-step process:first,the optical phase difference is encoded into a sub-GHz radiofrequency(RF)signal using microwave-photonic manipulation;then,RF interferometry-assisted phase amplification is implemented at the destructive interference point.In our experiments,we demonstrate a phase sensitivity of 2.14 rad∕nm operating at 140 MHz using a miniature in-fiber Fabry-Pérot interferometer for sub-nanometer displacement sensing,which reveals a sensitivity magnification factor of 258.6.With further refinement,we anticipate that even higher enhancement factors can be achieved,paving the way for the development of cost-effective,ultrasensitive interferometry-based instruments for high-precision optical measurements. 展开更多
关键词 fiber-optic interferometer microwave photonics INTERFEROMETRY phase sensing radiofrequency interferometry
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MewCDNet: A Wavelet-Based Multi-Scale Interaction Network for Efficient Remote Sensing Building Change Detection
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作者 Jia Liu Hao Chen +5 位作者 Hang Gu Yushan Pan Haoran Chen Erlin Tian Min Huang Zuhe Li 《Computers, Materials & Continua》 2026年第1期687-710,共24页
Accurate and efficient detection of building changes in remote sensing imagery is crucial for urban planning,disaster emergency response,and resource management.However,existing methods face challenges such as spectra... Accurate and efficient detection of building changes in remote sensing imagery is crucial for urban planning,disaster emergency response,and resource management.However,existing methods face challenges such as spectral similarity between buildings and backgrounds,sensor variations,and insufficient computational efficiency.To address these challenges,this paper proposes a novel Multi-scale Efficient Wavelet-based Change Detection Network(MewCDNet),which integrates the advantages of Convolutional Neural Networks and Transformers,balances computational costs,and achieves high-performance building change detection.The network employs EfficientNet-B4 as the backbone for hierarchical feature extraction,integrates multi-level feature maps through a multi-scale fusion strategy,and incorporates two key modules:Cross-temporal Difference Detection(CTDD)and Cross-scale Wavelet Refinement(CSWR).CTDD adopts a dual-branch architecture that combines pixel-wise differencing with semanticaware Euclidean distance weighting to enhance the distinction between true changes and background noise.CSWR integrates Haar-based Discrete Wavelet Transform with multi-head cross-attention mechanisms,enabling cross-scale feature fusion while significantly improving edge localization and suppressing spurious changes.Extensive experiments on four benchmark datasets demonstrate MewCDNet’s superiority over comparison methods:achieving F1 scores of 91.54%on LEVIR,93.70%on WHUCD,and 64.96%on S2Looking for building change detection.Furthermore,MewCDNet exhibits optimal performance on the multi-class⋅SYSU dataset(F1:82.71%),highlighting its exceptional generalization capability. 展开更多
关键词 Remote sensing change detection deep learning wavelet transform MULTI-SCALE
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GLMCNet: A Global-Local Multiscale Context Network for High-Resolution Remote Sensing Image Semantic Segmentation
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作者 Yanting Zhang Qiyue Liu +4 位作者 Chuanzhao Tian Xuewen Li Na Yang Feng Zhang Hongyue Zhang 《Computers, Materials & Continua》 2026年第1期2086-2110,共25页
High-resolution remote sensing images(HRSIs)are now an essential data source for gathering surface information due to advancements in remote sensing data capture technologies.However,their significant scale changes an... High-resolution remote sensing images(HRSIs)are now an essential data source for gathering surface information due to advancements in remote sensing data capture technologies.However,their significant scale changes and wealth of spatial details pose challenges for semantic segmentation.While convolutional neural networks(CNNs)excel at capturing local features,they are limited in modeling long-range dependencies.Conversely,transformers utilize multihead self-attention to integrate global context effectively,but this approach often incurs a high computational cost.This paper proposes a global-local multiscale context network(GLMCNet)to extract both global and local multiscale contextual information from HRSIs.A detail-enhanced filtering module(DEFM)is proposed at the end of the encoder to refine the encoder outputs further,thereby enhancing the key details extracted by the encoder and effectively suppressing redundant information.In addition,a global-local multiscale transformer block(GLMTB)is proposed in the decoding stage to enable the modeling of rich multiscale global and local information.We also design a stair fusion mechanism to transmit deep semantic information from deep to shallow layers progressively.Finally,we propose the semantic awareness enhancement module(SAEM),which further enhances the representation of multiscale semantic features through spatial attention and covariance channel attention.Extensive ablation analyses and comparative experiments were conducted to evaluate the performance of the proposed method.Specifically,our method achieved a mean Intersection over Union(mIoU)of 86.89%on the ISPRS Potsdam dataset and 84.34%on the ISPRS Vaihingen dataset,outperforming existing models such as ABCNet and BANet. 展开更多
关键词 Multiscale context attention mechanism remote sensing images semantic segmentation
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Single-shot wavefront sensing enabled by a photonic integrated circuit
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作者 Wenyu Chen Zixin Zhao +3 位作者 Shiyuan Liu Hui Deng Liang Gao Jinlong Zhu 《Advanced Photonics Nexus》 2026年第1期131-139,共9页
Point-of-care diagnostics and inline quantitative phase imaging(QPI)drive the demand for portable,ultra-miniaturized,and robust optical imaging and metrology systems.We propose and demonstrate a wavefront sensor integ... Point-of-care diagnostics and inline quantitative phase imaging(QPI)drive the demand for portable,ultra-miniaturized,and robust optical imaging and metrology systems.We propose and demonstrate a wavefront sensor integrated into a photonic integrated circuit,enabling single-shot optical phase retrieval.We implemented an integrated wavefront sensor array with a spatial resolution of 17μm and a numerical aperture of 0.1.Furthermore,we experimentally demonstrated the reconstruction of wavefronts defined by Zernike polynomials,specifically the first 14 terms(Z_(1)to Z_(14)),achieving an average root mean square error below 0.07.This advancement paves the way for fully integrated,portable,and robust optical imaging systems,facilitating integrated wavefront sensors in demanding applications such as point-of-care diagnostics,endoscopy,in situ QPI,and inline surface profile measurement. 展开更多
关键词 wavefront sensing photonic integrated circuit computational imaging miniaturized optical system
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Research Progress on Spatiotemporal Variability of Rice Planting Based on Satellite Remote Sensing Monitoring
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作者 Qi ang HU Aichuan LI +2 位作者 Xinbing WANG Francesco Marinello Zhan SHI 《Agricultural Biotechnology》 2026年第1期76-81,共6页
As a vital food crop,rice is an important part of global food crops.Studying the spatiotemporal changes in rice cultivation facilitates early prediction of production risks and provides support for agricultural policy... As a vital food crop,rice is an important part of global food crops.Studying the spatiotemporal changes in rice cultivation facilitates early prediction of production risks and provides support for agricultural policy decisions related to rice.With the increasing application of satellite remote sensing technology in crop monitoring,remote sensing for rice cultivation has emerged as a novel approach,offering new perspectives for monitoring rice planting.This paper briefly outlined the current research and development status of satellite remote sensing for monitoring rice cultivation both at home and abroad.Foreign scholars have made innovations in data sources and methodologies for satellite remote sensing monitoring,and utilized multi-source satellite information and machine learning algorithms to enhance the accuracy of rice planting monitoring.Scholars in China have achieved significant results in the study of satellite remote sensing for monitoring rice cultivation.Their research and application in monitoring rice planting areas provide valuable references for agricultural production management.However,satellite remote sensing monitoring of rice still faces challenges such as low spatiotemporal resolution and difficulties related to cloud cover and data fusion,which require further in-depth investigation.Additionally,there are shortcomings in the accuracy of remote sensing monitoring for fragmented farmland plots and smallholder farming.To address these issues,future efforts should focus on developing multi-source heterogeneous data fusion analysis technologies and researching monitoring systems.These advancements are expected to enable high-precision large-scale acquisition of rice planting information,laying a foundation for future smart agriculture. 展开更多
关键词 Satellite remote sensing Rice cultivation Spatiotemporal variability MONITORING Research review
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Remote Sensing-Enhanced Lithological Mapping for Predicting Shallow Landslide Susceptibility in Complex Terrains
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作者 Qixing Wang 《Journal of Environmental & Earth Sciences》 2026年第3期251-265,共15页
Shallow landslides are strongly controlled by near-surface lithological variability,yet conventional geological maps are often too generalized to support accurate susceptibility assessment in complex terrains.This rev... Shallow landslides are strongly controlled by near-surface lithological variability,yet conventional geological maps are often too generalized to support accurate susceptibility assessment in complex terrains.This review synthesizes recent advances in remote sensing–based lithological mapping and evaluates their integration into landslide susceptibility modeling.Evidence from the literature indicates that remote sensing-derived lithological products,particularly those incorporating mineralogical information and higher spatial resolution,consistently outperform traditional geological maps in improving model accuracy and spatial detail,especially in heterogeneous environments.However,key challenges remain,including scale mismatches between surface observations and subsurface controls,limited ground validation,uncertainty propagation,and restricted model transferability across regions.The review identifies multi-sensor data fusion and explainable machine learning as the most promising directions for advancing lithological discrimination and model reliability.Future progress depends on integrating remote sensing with process-based understanding,improving validation strategies,and standardizing uncertainty reporting.These developments are essential for enabling more robust,scalable,and operationally relevant landslide susceptibility assessments in complex terrains.Lastly,we describe the directions of research that focus on multi-sensor fusion,explainable machine learning,UAV(Unmanned Aerial Vehicle)-enabled validation,and standardized uncertainty reporting that can help articulate landslide susceptibility assessment,making them even more robust and operationally significant. 展开更多
关键词 Shallow Landslides Lithological Mapping Remote sensing Susceptibility Modeling Complex Terrain
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Enhanced Multi-Scale Feature Extraction Lightweight Network for Remote Sensing Object Detection
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作者 Xiang Luo Yuxuan Peng +2 位作者 Renghong Xie Peng Li Yuwen Qian 《Computers, Materials & Continua》 2026年第3期2097-2118,共22页
Deep learning has made significant progress in the field of oriented object detection for remote sensing images.However,existing methods still face challenges when dealing with difficult tasks such as multi-scale targ... Deep learning has made significant progress in the field of oriented object detection for remote sensing images.However,existing methods still face challenges when dealing with difficult tasks such as multi-scale targets,complex backgrounds,and small objects in remote sensing.Maintaining model lightweight to address resource constraints in remote sensing scenarios while improving task completion for remote sensing tasks remains a research hotspot.Therefore,we propose an enhanced multi-scale feature extraction lightweight network EM-YOLO based on the YOLOv8s architecture,specifically optimized for the characteristics of large target scale variations,diverse orientations,and numerous small objects in remote sensing images.Our innovations lie in two main aspects:First,a dynamic snake convolution(DSC)is introduced into the backbone network to enhance the model’s feature extraction capability for oriented targets.Second,an innovative focusing-diffusion module is designed in the feature fusion neck to effectively integrate multi-scale feature information.Finally,we introduce Layer-Adaptive Sparsity for magnitude-based Pruning(LASP)method to perform lightweight network pruning to better complete tasks in resource-constrained scenarios.Experimental results on the lightweight platform Orin demonstrate that the proposed method significantly outperforms the original YOLOv8s model in oriented remote sensing object detection tasks,and achieves comparable or superior performance to state-of-the-art methods on three authoritative remote sensing datasets(DOTA v1.0,DOTA v1.5,and HRSC2016). 展开更多
关键词 Deep learning object detection feature extraction feature fusion remote sensing
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Strain localization and time-dependent deformation in granodiorite characterized by distributed optical fiber sensing
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作者 Shuting Miao Arno Zang +3 位作者 Guido Blöcher Yinlin Ji Hannes Hofmann Pengzhi Pan 《Journal of Rock Mechanics and Geotechnical Engineering》 2026年第1期166-178,共13页
A multi-stage stress relaxation test was performed on a granodiorite sample to understand the deformation process prior to the macroscopic failure of brittle rocks,as well as the transient response during stress relax... A multi-stage stress relaxation test was performed on a granodiorite sample to understand the deformation process prior to the macroscopic failure of brittle rocks,as well as the transient response during stress relaxation.Distributed optical fiber sensing was used to measure strains across the sample surface by helically wrapping the single-mode fiber around the cylindrical sample.Close agreement was observed between the circumferential strains obtained from the optical fibers and the extensometer.The reconstructed full-field strain contours show strain heterogeneity from the crack closure phase,and the strains in the later deformation phase are dominantly localized within the former high-strain zone.The Gini coefficient was used to quantify the degree of strain localization and shows an initial increase during the crack closure phase,a decrease during the linear elastic phase,and a subsequent increase during the post-yielding phase.This behavior corresponds to a process of initial localization from an imperfect boundary condition,homogenization,and eventual relocalization prior to the macroscopic failure of the sample.The transient strain rate decay during the stress relaxation phase was quantified using the p-value in the“Omori-like"power law function.A higher initial stress at the onset of relaxation results in a lower p-value,indicating a slower strain rate decay.As the sample approaches macroscopic failure,the lowest p-value shifts from the most damaged zone to adjacent areas,suggesting stress redistribution or crack propagation in deformed crystalline rocks under stress relaxation conditions. 展开更多
关键词 Distributed optical fiber sensing Stress relaxation Strain localization Time-dependent deformation
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Remote Sensing Approaches to Track Climate-Induced Changes in Hydrological Systems
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作者 Zhenghao Han Zhigang Ye +1 位作者 Ying Zhou Yin Cao 《Journal of Environmental & Earth Sciences》 2026年第1期202-223,共22页
Climate change is rapidly altering hydrological systems through changes in precipitation patterns,increase the rate of glacier retreat rates,altered snow dynamics,and groundwater stress.Although remote sensing has bee... Climate change is rapidly altering hydrological systems through changes in precipitation patterns,increase the rate of glacier retreat rates,altered snow dynamics,and groundwater stress.Although remote sensing has been extensively deployed in hydrological research,existing reviews typically focus on a single hydrological variable or on particular satellite missions.The review synthesizes remote sensing technologies to monitor climate-related hydrological variations across various components of the water cycle.It is a systematic examination of major satellite missions,sensor technologies,and analytical methods used to monitor precipitation,soil moisture,snow cover,surface water processes,and groundwater variability.The review will employ a structured literature review methodology,focusing on recent peer-reviewed articles that apply optical,microwave,radar,and gravimetric remote sensing methods for hydrological monitoring under changing climatic conditions.It has paid specific attention to the provision of the comparative capabilities,spatial-temporal resolutions,and practical applications of key satellite missions,such as Landsat,Sentinel,MODIS(Moderate Resolution Imaging Spectroradiometer),GPM(Global Precipitation Measurement),and GRACE(Gravity Recovery and Climate Experiment).Moreover,to illustrate the use of remote sensing in detecting glacier retreat,drought formation,and coastal groundwater salinization,regional case studies are selected and analyzed.The review identifies new opportunities to use multi-sensor data,machine learning,and high-resolution monitoring to enhance hydrological analyses.This study is useful in practice by synthesizing existing technological opportunities and research trends to enhance climate-responsive water resource monitoring and by outlining future research directions in remote sensing-based hydrological analysis. 展开更多
关键词 Remote sensing Climate Change Hydrological Systems Water Resource Management Satellite Monitoring
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