Chemical systems that can replicate cellular behaviors are gaining increasing attention and are being used to study various biological processes.Here,a protein-or amylose-based assembly at an oil/water interface was e...Chemical systems that can replicate cellular behaviors are gaining increasing attention and are being used to study various biological processes.Here,a protein-or amylose-based assembly at an oil/water interface was employed to construct a large compartmentalized adipocyte-like structure,and a lipid droplet-like microcompartment(amylose-polymerstabilized 2-ethyl-1-hexanol microcompartment),which exhibits floating behaviors driven by the amylose-polymer micelle solubilization,was introduced inside these adipocyte-like structures.When the lipase-based catalytic reaction and the external oil composition were controlled and another lipid droplet-like microcompartment(lipase-stabilized tributyrin microcompartment)was introduced,then a series of dynamic behaviors were exhibited by the two types of lipid droplet-like microcompartments,including growth,self-sorting,and fusion within the adipocyte-like structures.Therefore,it is anticipated that this autonomous generation of hierarchical multicompartments represents a breakthrough compared to the traditional bottom-up strategies,and the artificial life systems we studied could contribute a chemical-based strategy of understanding the process of lipid droplet growth and fusion inside a living adipocyte cell.展开更多
基金supported financially by the NSFC(nos.51873050 and 21871069)the China Postdoctoral Science Foundation(no.2015M571401).
文摘Chemical systems that can replicate cellular behaviors are gaining increasing attention and are being used to study various biological processes.Here,a protein-or amylose-based assembly at an oil/water interface was employed to construct a large compartmentalized adipocyte-like structure,and a lipid droplet-like microcompartment(amylose-polymerstabilized 2-ethyl-1-hexanol microcompartment),which exhibits floating behaviors driven by the amylose-polymer micelle solubilization,was introduced inside these adipocyte-like structures.When the lipase-based catalytic reaction and the external oil composition were controlled and another lipid droplet-like microcompartment(lipase-stabilized tributyrin microcompartment)was introduced,then a series of dynamic behaviors were exhibited by the two types of lipid droplet-like microcompartments,including growth,self-sorting,and fusion within the adipocyte-like structures.Therefore,it is anticipated that this autonomous generation of hierarchical multicompartments represents a breakthrough compared to the traditional bottom-up strategies,and the artificial life systems we studied could contribute a chemical-based strategy of understanding the process of lipid droplet growth and fusion inside a living adipocyte cell.
