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"Three Lines One Permit"environmental policy and its implementation assessment:a case study of Sichuan Province,China
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作者 LIU Yanguo WANG Li +5 位作者 YANG Ye LI Jingji PEI Xiangjun WANG Ziqin HE Yu CHEN An 《Journal of Mountain Science》 2026年第1期311-326,共16页
Integrated environmental management is important for sustainable development.Under China’s“Three Lines One Permit”(TLOP)policy,three types of management zones—priority protection,critical control,and general contr... Integrated environmental management is important for sustainable development.Under China’s“Three Lines One Permit”(TLOP)policy,three types of management zones—priority protection,critical control,and general control zones—are established based on the ecological red line,the lower-limit line for environmental quality,and the resource use line.Human activities are regulated through a permit system.Integrated and multifactorial protection of soil,plant,hydrological,and atmospheric elements is promoted at the regional level.A follow-up assessment contributes to the improvement of policy implementation and effectiveness.This study demonstrates the achievements of the TLOP policy in Sichuan Province.Results show that(1)276 protection zones have been established under the ecological red line,covering key ecosystems and protected areas to ensure environmental security.Under the lower-limit line,1,626 functional(priority,key,and general control)zones have been designated to regulate air,water,and soil quality,enhancing environmental capacity and pollution control.(2)Through the integration and merging of the three lines,1,128 integrated management zones have been established,including 375,625,and 128 priority protection,critical control,and general control zones,respectively.Each zone has its own list of environmental permits to regulate human activities according to different environmental protection and natural resource development regimes.(3)The design of the follow-up assessment index system was informed by regional primary functions and industrial structure.The index system for provinces and cities is structured around three primary indicators—implementation updating,application,and guarantees—and 15 secondary indicators.The system for critical control zones is structured around environmental access,management,and effectiveness and 14 secondary indicators.A stringent environmental zoning system has been established through the TLOP policy,thereby safeguarding environmental security,promoting harmonious existence between humans and nature,and supporting the vision of Beautiful China. 展开更多
关键词 Three lines One Permit Environment zoning system Ecological red line Environment assessment Southwest China
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Deep Learning-Based Toolkit Inspection:Object Detection and Segmentation in Assembly Lines
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作者 Arvind Mukundan Riya Karmakar +1 位作者 Devansh Gupta Hsiang-Chen Wang 《Computers, Materials & Continua》 2026年第1期1255-1277,共23页
Modern manufacturing processes have become more reliant on automation because of the accelerated transition from Industry 3.0 to Industry 4.0.Manual inspection of products on assembly lines remains inefficient,prone t... Modern manufacturing processes have become more reliant on automation because of the accelerated transition from Industry 3.0 to Industry 4.0.Manual inspection of products on assembly lines remains inefficient,prone to errors and lacks consistency,emphasizing the need for a reliable and automated inspection system.Leveraging both object detection and image segmentation approaches,this research proposes a vision-based solution for the detection of various kinds of tools in the toolkit using deep learning(DL)models.Two Intel RealSense D455f depth cameras were arranged in a top down configuration to capture both RGB and depth images of the toolkits.After applying multiple constraints and enhancing them through preprocessing and augmentation,a dataset consisting of 3300 annotated RGB-D photos was generated.Several DL models were selected through a comprehensive assessment of mean Average Precision(mAP),precision-recall equilibrium,inference latency(target≥30 FPS),and computational burden,resulting in a preference for YOLO and Region-based Convolutional Neural Networks(R-CNN)variants over ViT-based models due to the latter’s increased latency and resource requirements.YOLOV5,YOLOV8,YOLOV11,Faster R-CNN,and Mask R-CNN were trained on the annotated dataset and evaluated using key performance metrics(Recall,Accuracy,F1-score,and Precision).YOLOV11 demonstrated balanced excellence with 93.0%precision,89.9%recall,and a 90.6%F1-score in object detection,as well as 96.9%precision,95.3%recall,and a 96.5%F1-score in instance segmentation with an average inference time of 25 ms per frame(≈40 FPS),demonstrating real-time performance.Leveraging these results,a YOLOV11-based windows application was successfully deployed in a real-time assembly line environment,where it accurately processed live video streams to detect and segment tools within toolkits,demonstrating its practical effectiveness in industrial automation.The application is capable of precisely measuring socket dimensions by utilising edge detection techniques on YOLOv11 segmentation masks,in addition to detection and segmentation.This makes it possible to do specification-level quality control right on the assembly line,which improves the ability to examine things in real time.The implementation is a big step forward for intelligent manufacturing in the Industry 4.0 paradigm.It provides a scalable,efficient,and accurate way to do automated inspection and dimensional verification activities. 展开更多
关键词 Tool detection image segmentation object detection assembly line automation Industry 4.0 Intel RealSense deep learning toolkit verification RGB-D imaging quality assurance
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GFRF R-CNN:Object Detection Algorithm for Transmission Lines
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作者 Xunguang Yan Wenrui Wang +3 位作者 Fanglin Lu Hongyong Fan Bo Wu Jianfeng Yu 《Computers, Materials & Continua》 SCIE EI 2025年第1期1439-1458,共20页
To maintain the reliability of power systems,routine inspections using drones equipped with advanced object detection algorithms are essential for preempting power-related issues.The increasing resolution of drone-cap... To maintain the reliability of power systems,routine inspections using drones equipped with advanced object detection algorithms are essential for preempting power-related issues.The increasing resolution of drone-captured images has posed a challenge for traditional target detection methods,especially in identifying small objects in high-resolution images.This study presents an enhanced object detection algorithm based on the Faster Regionbased Convolutional Neural Network(Faster R-CNN)framework,specifically tailored for detecting small-scale electrical components like insulators,shock hammers,and screws in transmission line.The algorithm features an improved backbone network for Faster R-CNN,which significantly boosts the feature extraction network’s ability to detect fine details.The Region Proposal Network is optimized using a method of guided feature refinement(GFR),which achieves a balance between accuracy and speed.The incorporation of Generalized Intersection over Union(GIOU)and Region of Interest(ROI)Align further refines themodel’s accuracy.Experimental results demonstrate a notable improvement in mean Average Precision,reaching 89.3%,an 11.1%increase compared to the standard Faster R-CNN.This highlights the effectiveness of the proposed algorithm in identifying electrical components in high-resolution aerial images. 展开更多
关键词 Faster R-CNN transmission line object detection GIOU GFR
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基于自适应EDLines和鲁棒位姿优化的视觉SLAM算法
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作者 王浩森 张丽杰 《计算机仿真》 2025年第9期355-361,共7页
同时定位与地图构建(SLAM)广泛应用于无人驾驶、机器人等多个领域,为了提高ORB-SLAM3在光照不均和弱纹理场景中的定位精度和鲁棒性,提出了一种基于自适应EDLines和鲁棒位姿优化的视觉SLAM算法。首先,提出了一种自适应EDLines线段检测算... 同时定位与地图构建(SLAM)广泛应用于无人驾驶、机器人等多个领域,为了提高ORB-SLAM3在光照不均和弱纹理场景中的定位精度和鲁棒性,提出了一种基于自适应EDLines和鲁棒位姿优化的视觉SLAM算法。首先,提出了一种自适应EDLines线段检测算法,根据像素梯度的变化更新梯度阈值,并设计相似线段删除和分割线段合并策略,提高EDLines提取线特征的准确性和完整性。其次,提出一种与线段端点无关的线特征残差计算方法,避免后端优化过程中不同视角对位姿估计结果的影响。实验结果表明,提出的视觉SLAM算法比ORB-SLAM3具有更高的定位精度和鲁棒性。 展开更多
关键词 同时定位与地图构建 线特征 线特征提取 位姿优化
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China’s Sea Lines of Communication Security: A Perspective from a Holistic Approach to National Security
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作者 Wang Xu 《China International Studies》 2025年第4期31-68,共38页
Sea lines of communication(SLOCs)security has long been a strategic concern for major powers.Following the establishment of the People’s Republic of China,the country’s focus was on the traditional security aspects ... Sea lines of communication(SLOCs)security has long been a strategic concern for major powers.Following the establishment of the People’s Republic of China,the country’s focus was on the traditional security aspects of its SLOCs.Since the reform and opening-up era-and especially after the end of the Cold War-China has shifted its emphasis toward economic security.China’s SLOCs security bears on multiple dimensions of a holistic approach to national security,encompassing economic security,the safety of its citizens. 展开更多
关键词 holistic approach economic securitythe citizen safety sea lines communication slocs security national security sea lines communication security economic security
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Evaluation of transgenic cotton lines expressing an insecticidal fern protein against whitefly,Bemisia tabaci(Gennadius)
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作者 KUMAR Rishi NAGRARE V.S. +14 位作者 SHAH Vivek SINGH Satnam PANDHER Suneet SINGH Satpal VERMA S.K. PAUL Debashis RATHORE Pankaj SHUKLA Anoop Kumar SINGH Mithlesh Kumar SAURABH Sharad KUMAR Harish KAUR Rupinderjeet SINGH Pradhyumna Kumar WAGHMARE V.N. PRASAD Y.G. 《Journal of Cotton Research》 2025年第2期163-177,共15页
Background Transgenic research in crops involves using genetic engineering techniques to introduce specific genes of interest from other organisms,or even entirely new genes into plant genomes to create crops with des... Background Transgenic research in crops involves using genetic engineering techniques to introduce specific genes of interest from other organisms,or even entirely new genes into plant genomes to create crops with desirable traits that wouldn’t be possible through conventional breeding methods.Transgenic crops have been developed for various traits globally.Whitefly,Bemisia tabaci(Gennadius)is one of the major sucking pests of cotton that cause significant damage to the cotton production.To combat whitefly infestations,researchers have developed four transgenic cotton lines expressing the fern protein.And those transgenic lines need to be evaluated for their performance against the target pest—whitefly.The evaluation was designed as controlled trials in polyhouse or muslin cloth cages under open-choice and no-choice conditions by comparing four transgenic cotton lines(A,B,C,and D)with three control groups,including untransformed cotton plants with a same genetic background of the transgenic line,conventionally bred whitefly-resistant cotton,and whitefly-susceptible cotton.In order to study the generational effect,the evaluation also involved studies on whitefly development in laboratory,muslin cloth cage,and polyhouse conditions.Results Both open-choice and no-choice experiments had shown that all the four transgenic cotton lines(A,B,C,and D)expressing the fern protein reduced adult whitefly numbers significantly compared with the control lines,except for the no-choice conditions in 2021,where the transgenic line C was non-significant different from the resistant control line.Notably,the nymphal population on the resistant control line was relatively low and nonsignificant different from the transgenic line C in 2021;and the transgenic lines A and C in 2022 under open-choice conditions.Under no-choice condition,the nymphal counts in the resistant control line was non-significant different from transgenic lines C and D in 2021;and transgenic line D in 2022.All transgenic lines showed significant decrease in egg hatching in 2021 and nymphal development in 2022,except for the transgenic line C which had no significant different in the nymphal development comparing with non-transgenic control lines in 2022.Adult emergence rates in both years of evaluation showed significant decrease in transgenic lines A and B comparing with the control lines.Additionally,the results showed a significant reduction in cotton leaf curl disease and sooty mold development in all the four transgenic lines compared with susceptible control under open-choice conditions,indicating potential benefits of transgenic lines beyond direct effect on whitefly control.Furthermore,the research explored the generational effects of the fern protein on whitefly which revealed the lowest fecundity in the transgenic line C across F0,F1 and F3 generations,lower egg hatching in F1 and F2 generations in transgenic lines A and B,shorter nymphal duration in F1 and F2 generations in transgenic line B,and the least total adult emergence in the transgenic line C in F0 and F3 generations.Conclusions These findings suggest that the transgenic cotton lines expressing fern protein disrupts whitefly populations and the life cycle to a certain extent.However,results are not consistent over generations and years of study,indicating these transgenic lines were not superior over control lines and need to be improved in future breeding. 展开更多
关键词 EVALUATION Fern protein Transgenic cotton lines WHITEFLY
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A Study of Multiple Molecular Lines at the 3 mm Band toward Gas Infalling Sources
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作者 Yang Yang Xi Chen +3 位作者 Zhibo Jiang Zhiwei Chen Shuling Yu Jun Li 《Research in Astronomy and Astrophysics》 2025年第5期230-245,共16页
The study of multiple molecular spectral lines in gas infalling sources can provide the physical and chemica properties of these sources and help us estimate their evolutionary stages.We report line detections within ... The study of multiple molecular spectral lines in gas infalling sources can provide the physical and chemica properties of these sources and help us estimate their evolutionary stages.We report line detections within the 3 mm band using the FTS wide-sideband mode of the IRAM 30 m telescope toward 20 gas-infalling sources.Using XCLASS,we identify the emission lines of up to 22 molecular species(including a few isotopologues)and on hydrogen radio recombination line in these sources.H^(13)CO^(+),HCO^(+),HCN,HNC,c-C_(3)H_(2),and CCH lines are detected in 15 sources.We estimate the rotation temperatures and column densities of these molecular species using the LTE radiative transfer model,and compare the molecular abundances of these sources with those from nine high mass star-forming regions reported in previous studies and with those from the chemical model.Our results sugges that G012.79-0.20,G012.87-0.22 clump A and B,and G012.96-0.23 clump A may be in the high-mass protostella object stage,while sources with fewer detected species may be in the earlier evolutionary stage.Additionally,th CCH and c-C_(3)H_(2)column densities in our sources reveal a linear correlation,with a ratio of N(CCH)/N(c-C_(3)H_(2)=89.2±5.6),which is higher than the ratios reported in the literature.When considering only sources with lowe column densities,this ratio decreases to 29.0±6.1,consistent with those of diffuse clouds.Furthermore,comparison between the N(CCH)/N(c-C_(3)H_(2))ratio and the sources’physical parameters reveals a correlation,with sources exhibiting higher ratios tending to have higher kinetic temperatures and H2column densities. 展开更多
关键词 GALAXIES star formation-ISM clouds-ISM lines and bands-ISM MOLECULES
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Development of Gossypium hirsutum–Gossypium raimondii introgression lines and their use in QTL mapping of agricultural traits
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作者 Liang Wang Nijiang Ai +12 位作者 Zechang Zhang Chenhui Zhou Guoli Feng Sheng Cai Ningshan Wang Liuchun Feng Yu Chen Min Xu Yingying Wang Haoran Yue Mengfei Chen Liangshuai Xing Baoliang Zhou 《Journal of Integrative Agriculture》 2025年第5期1688-1703,共16页
Gossypium raimondii(2n=2x=26,D_(5)),an untapped wild species,is the putative progenitor of the D-subgenome of G.hirsutum(2n=4x=52,AD_(1)),an extensively cultivated species.Here,we developed a G.hirsutum(recipient)–G.... Gossypium raimondii(2n=2x=26,D_(5)),an untapped wild species,is the putative progenitor of the D-subgenome of G.hirsutum(2n=4x=52,AD_(1)),an extensively cultivated species.Here,we developed a G.hirsutum(recipient)–G.raimondii(donor)introgression population to exploit the favorable QTLs/genes and mapped potential quantitative trait loci(QTLs)from wild cotton species.The introgression population consisted of 256 lines with an introgression rate of 52.33%for the G.raimondii genome.The introgression segment length range was 0.03–19.12 Mb,with an average of 1.22 Mb.The coverage of total introgression fragments from G.raimondii was 386.98 Mb.Further genome-wide association analysis(Q+K+MLM)and QTL mapping(RSTEP-LRT)identified 59 common QTLs,including 14 stable QTLs and six common QTL(co-QTL)clusters,and one hotspot of micronaire(MIC).The common QTLs for seed index all showed positive additive effects,while the common QTLs for boll weight all had negative additive effects,indicating that the linkage between seed index and boll weight could be broken.QTLs for lint percentage showed positive effects and could be beneficial for improving cotton yield.Most QTLs for fiber quality had negative additive effects,implying these QTLs were domesticated/improved in G.hirsutum.A few fiber quality QTLs showed positive additive effects,so they could be used to improve cotton fiber quality.The introgression lines developed could be useful for molecular marker-assisted breeding and mapping QTLs precisely for mining desirable genes from the wild species G.raimondii.Such genes can improve cultivated cotton in the future through a designbreeding approach. 展开更多
关键词 Gossypium hirsutum Gossypium raimondii introgression lines YIELD fiber quality quantitative trait locus
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ISOPERIMETRIC INEQUALITIES FOR INTEGRAL GEOMETRIC INVARIANTS OF RANDOM LINES
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作者 Gaoyong ZHANG 《Acta Mathematica Scientia》 2025年第1期189-199,共11页
Isoperimetric type inequalities for integral geometric invariants of random lines in the Euclidean space are shown.Entropy inequalities of probability densities on the affine Grassmann manifold of lines are given.
关键词 isoperimetric inequality convex body random points random lines chord integral Riesz potential ENTROPY
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Introgressed eggplant lines with the wild Solanum incanum evaluated under drought stress conditions
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作者 Martín Flores-Saavedra Pietro Gramazio +5 位作者 Santiago Vilanova Diana M.Mircea Mario X.Ruiz-González Óscar Vicente Jaime Prohens Mariola Plazas 《Journal of Integrative Agriculture》 2025年第6期2203-2216,共14页
As access to irrigation water becomes increasingly limited, introgression of relevant genomic regions from drought-tolerant wild genotypes is a promising breeding strategy for crop plants. In this study, nine eggplant... As access to irrigation water becomes increasingly limited, introgression of relevant genomic regions from drought-tolerant wild genotypes is a promising breeding strategy for crop plants. In this study, nine eggplant(Solanum melongena) introgression lines(ILs) covering altogether 71.6% of the genome of the donor wildrelative parent S. incanum were evaluated for drought tolerance under water stress conditions. Plants at the five true leaves stage were irrigated at either 100%(control) or 30%(water stress) field capacity for 14 days, and growth and biochemical traits were measured. Reduced irrigation resulted in decreased growth and increased stress markers such as proline and malondialdehyde. Most ILs had lower growth and biomass production thanthe cultivated parent under both conditions. However, the wild alleles for two genomic regions related to stem and root dry weight conferred improved tolerance to water stress. In addition, several S. incanum alleles had a positive effect on important traits that may improve yield under drought conditions, such as leaf water content water use efficiency, and chlorophyll content. Fine-mapping the genomic regions for tolerance and reducinglinkage drag with regions affecting growth will be crucial for significantly improving eggplant drought tolerance through introgression breeding. 展开更多
关键词 Solanum melongena S.incanum introgression lines water stress oxidative stress genomic regions
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Application of AI Intelligence in Module PACK Lines
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作者 HU Jian LONG Mingsheng QIN Qianbin 《International Journal of Plant Engineering and Management》 2025年第2期80-96,共17页
With the rapid development of the new energy automotive industry,the enhancement of lithium battery performance and production efficiency has become critical.This article explores the application of artificial intelli... With the rapid development of the new energy automotive industry,the enhancement of lithium battery performance and production efficiency has become critical.This article explores the application of artificial intelligence technology in the lithium battery module PACK line,analyzing how it optimizes the production process and improves production efficiency,and predicts future development trends.The PACK line is an important link in battery manufacturing,involving complex processes such as cell sorting,welding,assembly and testing.The application of AI technology in image recognition,data analysis and predictive maintenance provides new solutions for the intelligent upgrading of the PACK line.This article describes the process of the PACK line in detail,analyzes the challenges under current technological levels,and reviews the application cases of AI technology in the manufacturing industry.The study aims to provide theoretical and practical guidance for the intelligent development of lithium battery module PACK lines,discussing the integration of AI technology,its actual performance,technical challenges,and solutions.It is expected that AI technology will play a greater role in the PACK line,and future research will focus on improving the adaptability of models,developing efficient algorithms,and further integrating into the production line. 展开更多
关键词 artificial intelligence module PACK line production efficiency predictive maintenance image recognition intelligent manufacturing
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Sharp Bounds for ABS Index of Line,Total and Mycielski Graphs
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作者 YE Qingfang LI Fengwei 《数学进展》 北大核心 2026年第1期45-59,共15页
The atom-bond sum-connectivity(ABS)index,put forward by[J.Math.Chem.,2022,60(10):20812093],exhibits a strong link with the acentric factor of octane isomers.The experimental physico-chemical properties of octane isome... The atom-bond sum-connectivity(ABS)index,put forward by[J.Math.Chem.,2022,60(10):20812093],exhibits a strong link with the acentric factor of octane isomers.The experimental physico-chemical properties of octane isomers,such as boiling point,of formation are found to be better measured by the ABS index than by the Randi,atom-bond connectivity(ABC),and sum-connectivity(SC)indices.One important source of information for researching the molecular structure is the bounds for its topological indices.The extrema of the ABS index of the line,total,and Mycielski graphs are calculated in this work.Moreover,the pertinent extremal graphs were illustrated. 展开更多
关键词 ABS index line graph total graph Mycielski graph
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Optically-excited acoustic waves in Si nanowires probed by time-resolved HOLZ lines
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作者 He Wang Shuaishuai Sun +7 位作者 Yizhe Wang Qianming An Xianhui Ye Jun Li Huanfang Tian Huaixin Yang Jianqi Li Zian Li 《Chinese Physics B》 2025年第9期663-669,共7页
Exploring advanced techniques capable of probing nanometric acoustic waves in nanostructures is critically important for the development of miniaturized acoustic devices.In this study,we probe the optically-excited ac... Exploring advanced techniques capable of probing nanometric acoustic waves in nanostructures is critically important for the development of miniaturized acoustic devices.In this study,we probe the optically-excited acoustic waves in a single silicon nanowire(NW)using the time-resolved(tr-)high-order Laue-zone(HOLZ)lines under convergent-beam electron diffraction(CBED)conditions in an ultrafast transmission electron microscope(UTEM).We devise an experimental scheme to obtain tr-HOLZ lines under off-zone-axis CBED conditions.We also propose a geometric description of HOLZ line formation and use this alternative description to quantitatively evaluate the dynamics of optically-excited silicon NW.Using part of the deformation gradient tensor,our simulations of the dynamics of Si NW reproduce the experimental results.We further discuss the feasibility of a full retrieval of the deformation gradient tensor by using a set of HOLZ lines from three zone axes.Our findings illustrate a strategy for the quantitative access to dynamical acoustic waves optically excited in micro-and nano-structures using UTEM. 展开更多
关键词 ultrafast transmission electron microscope high-order Laue-zone lines pulsed laser excitation acoustic waves
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Vibration suppression analysis of iced transmission lines under axial time-delay velocity feedback strategy
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作者 Maoming Hu Guangyun Min +3 位作者 Wanyu Bao Bowen Tian Shuguang Yang Mengqi Cai 《Theoretical & Applied Mechanics Letters》 2025年第1期69-86,共18页
To ensure the safety of power energy transmission channel and mitigate the harm caused by galloping of iced transmission lines,the axial time-delay velocity feedback strategy is adopted to suppress the galloping.The p... To ensure the safety of power energy transmission channel and mitigate the harm caused by galloping of iced transmission lines,the axial time-delay velocity feedback strategy is adopted to suppress the galloping.The par-tial differential equation of galloping with axial time-delay velocity feedback strategy is established based on the variational principle for Hamiltonian.Then,the partial differential equation of galloping is transformed into or-dinary differential equation based on normalization and the Galerkin method.The primary amplitude-frequency response equation,the first-order steady-state approximate solution,and the harmonic amplitude-frequency re-sponse equation are derived by the multiscale method.The impact of different parameters such as time-delay value,control coefficient,and amplitude of external excitation on the galloping response are analyzed.The am-plitude under the primary resonance exhibits periodicity as time-delay value varies.The amplitude diminishes with increased control coefficient and increases with external excitation.Comprehensive consideration of vari-ous influences of parameters on vibration characteristics is crucial when employing the axial time-delay velocity feedback strategy to suppress galloping.Therefore,to achieve the best vibration suppression effect,it is crucial to adjust the time-delay parameter for modifying the range and amplitude of the resonance zone.The conclusions obtained by this study are expected to advance the refinement of active control techniques for iced transmission lines,and may provide valuable insights for practical engineering applications. 展开更多
关键词 Iced transmission lines Galloping characteristics Axial time-delay velocity feedback STRATEGY Primary resonance Harmonic resonance
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On-line SPE/LC-LIT-MS法快速测定全血和肝组织中的利多卡因及其代谢物
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作者 卢敏萍 莫旖 +4 位作者 何日梅 黄克建 刘晓锋 杨宁 乔文涛 《化学研究与应用》 北大核心 2026年第1期228-233,共6页
基于在线固相萃取(on-line SPE)前处理技术,建立了一种快速测定全血和肝组织样品中利多卡因(LDC)及其代谢物单乙基甘氨酰二甲基苯胺(MEGX)、甘氨酰二甲基苯胺(GX)的液相色谱-线性离子阱质谱(LC-LIT-MS)分析方法。用乙腈沉淀样品中的蛋白... 基于在线固相萃取(on-line SPE)前处理技术,建立了一种快速测定全血和肝组织样品中利多卡因(LDC)及其代谢物单乙基甘氨酰二甲基苯胺(MEGX)、甘氨酰二甲基苯胺(GX)的液相色谱-线性离子阱质谱(LC-LIT-MS)分析方法。用乙腈沉淀样品中的蛋白,再经稀释、离心、过滤后即可进样。采用Waters Oasis®HLB在线SPE柱对样品进行快速富集纯化,结束后,通过阀切换将样品转移至Syncronis C8分析柱进行梯度洗脱,流动相为均含0.1%甲酸(V/V)的0.01 mol·L^(-1)乙酸铵溶液(A)和甲醇(B),最后使用电喷雾电离正离子源(ESI^(+)),在多反应监测(MRM)模式下进行测定,对利多卡因及其代谢物实现定性筛查与定量分析。在考察的浓度范围内,三种分析物均获得了良好的线性关系,相关系数为0.998 3~0.999 6,全血样品的检出限为(0.1~2.0)ng·mL^(−1),肝组织样品的检出限为(0.1~2.0)ng·g^(-1),3个添加水平的回收率为94.0~108.6%,相对标准偏差为0.1~5.8%(n=6)。该方法避免了繁琐的前处理过程,简单灵敏,结果准确可靠,适用于全血和肝组织样品中利多卡因及其代谢物的快速测定。 展开更多
关键词 在线固相萃取 LC-LIT-MS法 利多卡因 代谢物 全血 肝组织
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Identification of a genomic region controlling broad-spectrum immunity to leaf rust in wheat–Agropyron cristatum 2P translocation lines
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作者 Xiajie Ji Shirui Xu +10 位作者 Zongyao Wang Haiming Han Jinpeng Zhang Shenghui Zhou Baojin Guo Xinming Yang Xiuquan Li Xiaomin Guo Taiguo Liu Lihui Li Weihua Liu 《Journal of Integrative Agriculture》 2025年第5期2015-2018,共4页
Wheat leaf rust,caused by Puccinia triticina(Pt),is one of the most devastating diseases in common wheat(Triticum aestivum L.)and can lead to heavy yield loss(Chai et al.2020).Leaf rust can result in 50%yield loss dur... Wheat leaf rust,caused by Puccinia triticina(Pt),is one of the most devastating diseases in common wheat(Triticum aestivum L.)and can lead to heavy yield loss(Chai et al.2020).Leaf rust can result in 50%yield loss during epidemic years(Huerta-Espino et al.2011;Gebrewahid et al.2020;Kolomiets et al.2021).Breeding varieties resistant to leaf rust have been recognized as the most effective and economical method to mitigate wheat losses caused by Pt.The narrow genetic basis of wheat constrains the number of cultivars resistant to leaf rust(Jin et al.2021). 展开更多
关键词 broad spectrum immunity leaf rustcaused Agropyron cristatum genomic region p translocation lines puccinia triticina pt leaf rust WHEAT
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Generation of a collection of MYB mutant lines via pooled CRISPR-Cas9 in grape
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作者 Xuena Yu Yang Hu +5 位作者 Jiasi Han Liang Zhao Zhuoshuai Jin Xiangnan Xu Jiayue Feng Yingqiang Wen 《Journal of Integrative Agriculture》 2025年第8期3287-3290,共4页
CRISPR-Cas9 has emerged as a powerful tool for gene editing,and it has been widely used in plant functional genomics research and crop genetic breeding(Chen et al.2019).The target specificity of CRISPR-Cas9 relies on ... CRISPR-Cas9 has emerged as a powerful tool for gene editing,and it has been widely used in plant functional genomics research and crop genetic breeding(Chen et al.2019).The target specificity of CRISPR-Cas9 relies on the 20-base-pair single guide RNA(sgRNA),which makes creating plant-specific mutant libraries through large-scale synthesis of sgRNAs targeting multiple genes or even the whole genome relatively quick and straightforward. 展开更多
关键词 plant functional genomics research GRAPE myb mutant lines plant functional genomics CRISPR Cas crop genetic breeding gene editingand gene editing
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Pacific International Lines:Navigating Digital Shipping With Chinese Partners
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《China Report ASEAN》 2025年第9期60-60,共1页
Singapore leverages its strategic location as a logistics and financial hub serving Southeast Asia to help companies enhance regional operations and build resilient supply chains,according to Teo Siong Seng,Chairman o... Singapore leverages its strategic location as a logistics and financial hub serving Southeast Asia to help companies enhance regional operations and build resilient supply chains,according to Teo Siong Seng,Chairman of the Singapore Business Federation and Executive Chairman of Pacific International Lines(PIL),at the“Singapore Night”event held on the sidelines of the 21st China-ASEAN Expo.He also expressed hopes to work hand in hand with Chinese enterprises to explore emerging areas of cooperation and realize a vision of mutual benefits. 展开更多
关键词 strategic location logistics hub financial hub Chinese partners build resilient supply chainsaccording pacific international lines pil digital shipping resilient supply chains
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Analysis of the Field Circuit Model for the Suspension Potential of Conductors in Single-Circuit Outage of Double-Circuit DC Transmission Lines on the Same Tower
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作者 Jilai Xu Baoquan Wan +2 位作者 Jun Zhao Jiangong Zhang Min Dai 《High Voltage》 2025年第6期1522-1531,共10页
In the de-icing process of double-circuit direct current(DC)transmission lines on the same tower,an operational condition exists in which one circuit is de-energised and utilised as part of the de-icing current path,s... In the de-icing process of double-circuit direct current(DC)transmission lines on the same tower,an operational condition exists in which one circuit is de-energised and utilised as part of the de-icing current path,specifically designed for grounding line de-icing purposes.Accurate calculation of the floating potential of the out-of-service line is crucial for the design of de-icing devices.This study employs the upstream finite element method combined with a potential prediction correction approach to determine the floating potential of de-energised conductors.A field-circuit model is proposed for calculating conductor floating potentials under single-circuit outage conditions in double-circuit DC transmission lines.The charge dissipation process through grounding resistors with varying values is also analysed.In a�500 kV case study,the relative errors between the circuit model and electric field model calculations for the positive and negative conductor floating potentials are merely 3.66%and 0.89%,respectively,verifying the validity of the circuit model.The equivalent resistance of ion flow fields reaches magnitudes of 106Ω,significantly exceeding the intrinsic resistance of the conductors.When using voltage-limiting resistors,conductor voltages decrease significantly with a 0.5-MΩresistor,and the calculated floating potentials become 8.702 kV and−5.849 kV,reflecting reductions of 84.8%and 88.4%,respectively.This research demonstrates that the proposed method provides an effective solution for floating potential calculation during single-circuit outages in double-circuit DC lines.The analysis of charge dissipation through voltage-limiting resistors offers technical support for designing DC de-icing devices. 展开更多
关键词 voltage limiting resistors prediction correction approach floating potential de icing upstream finite element method field circuit model double circuit dc transmission lines single circuit outage
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Lane Line Detection Method for Complex Road Scenes Based on DeepLabv3+and MobilenetV4
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作者 Yingkai Ge Jiasheng Zhang +3 位作者 Jiale Zhang Zhenguo Ma Yu Liu Lihua Wang 《Computers, Materials & Continua》 2026年第4期1341-1363,共23页
With the continuous development of artificial intelligence and computer vision technology,numerous deep learning-based lane line detection methods have emerged.DeepLabv3+,as a classic semantic segmentation model,has f... With the continuous development of artificial intelligence and computer vision technology,numerous deep learning-based lane line detection methods have emerged.DeepLabv3+,as a classic semantic segmentation model,has found widespread application in the field of lane line detection.However,the accuracy of lane line segmentation is often compromised by factors such as changes in lighting conditions,occlusions,and wear and tear on the lane lines.Additionally,DeepLabv3+suffers from high memory consumption and challenges in deployment on embedded platforms.To address these issues,this paper proposes a lane line detection method for complex road scenes based on DeepLabv3+and MobileNetV4(MNv4).First,the lightweight MNv4 is adopted as the backbone network,and the standard convolutions in ASPP are replaced with depthwise separable convolutions.Second,a polarization attention mechanism is introduced after the ASPP module to enhance the model’s generalization capability.Finally,the Simple Linear Iterative Clustering(SLIC)superpixel segmentation algorithmis employed to preserve lane line edge information.MNv4-DeepLabv3+was tested on the TuSimple and CULane datasets.On the TuSimple dataset,theMean Intersection over Union(MIoU)and Mean Pixel Accuracy(mPA)improved by 1.01%and 7.49%,respectively.On the CULane dataset,MIoU andmPA increased by 3.33%and 7.74%,respectively.Thenumber of parameters decreased from 54.84 to 3.19 M.Experimental results demonstrate that MNv4-DeepLabv3+significantly optimizes model parameter count and enhances segmentation accuracy. 展开更多
关键词 Deep learning lane line detection DeepLabv3+ MobileNetV4 SLIC
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