In situ precise detection of bioactive molecules with high sensitivity and spatiotemporal resolution is essential for studying physiological events and disease diagnosis.The utilization of versatile fluorescent probes...In situ precise detection of bioactive molecules with high sensitivity and spatiotemporal resolution is essential for studying physiological events and disease diagnosis.The utilization of versatile fluorescent probes in fluorescence imaging offers a powerful tool for in vivo imaging of biomarkers closely associated with pathological conditions.However,the dynamic behavior leading to rapid clearance of small molecule probes from regions of interest severely compromises their potential for precise imaging.Notably,self-immobilizing fluorescent probes that selectively recognize diseased tissues while improving in situ retention and enrichment enable accurate high-fidelity fluorescence imaging.In this review,we aim to summarize the strategies employed for recent advances in the performance and precision of in vivo fluorescence imaging using self-immobilizing techniques.Lastly,we discuss the prospects and potential challenges associated with selfimmobilizing fluorescent probes to promote further development and application of more delicate fluorescent probes.展开更多
Reactive oxygen species(ROS)are involved in the onset and development of neurodegenerative diseases,such as Alzheimer's disease(AD)and Parkinson's disease(PD).Hydrogen peroxide(H_(2)O_(2)),a key type of ROS,is...Reactive oxygen species(ROS)are involved in the onset and development of neurodegenerative diseases,such as Alzheimer's disease(AD)and Parkinson's disease(PD).Hydrogen peroxide(H_(2)O_(2)),a key type of ROS,is overexpressed in the early stages of AD and PD and is involved in the disease progression.Assessing H_(2)O_(2) levels in the brain is considered to be a valuable tool for detecting neurodegenerative diseases and exploring their pathogenesis.In this study,we developed two self-immobilizing PET tracers([^(18)F]HYAS and[^(18)F]HYAT)based on a quinone methide(QM)scaffold for non-invasive imaging of H_(2)O_(2) in the brain.Both tracers can respond to H_(2)O_(2) by forming a QM intermediate,which rapidly reacts with nucleophiles.[^(18)F]HYAT,with proper physicochemical properties,is able of crossing the blood-brain barrier.Increased uptake of[^(18)F]HYAT was observed in the brains of mice treated with 1-methyl-4-phenyl-l,2,3,6-tetrahydropyridine(MPTP),indicating that[^(18)F]HYAT is a useful tracer for PET imaging of H_(2)O_(2) in the brain.展开更多
基金support from the National Natural Science Foundation of China(No.22274005,U2167222)the Science Fund for Creative Research Groups of Nature Science Foundation of Hebei Province(B2021201038)+4 种基金Natural Science Foundation of Hebei Province(B2023201108)Hebei Province Higher Education Science and Technology Research Project(JZX2023001)Research and Innovation Team of Hebei University(IT2023A01)Hebei Province Innovation Capability Enhancement Plan Project(22567632H)Open Research Fund of School of Chemistry and Chemical Engineering,Henan Normal University.
文摘In situ precise detection of bioactive molecules with high sensitivity and spatiotemporal resolution is essential for studying physiological events and disease diagnosis.The utilization of versatile fluorescent probes in fluorescence imaging offers a powerful tool for in vivo imaging of biomarkers closely associated with pathological conditions.However,the dynamic behavior leading to rapid clearance of small molecule probes from regions of interest severely compromises their potential for precise imaging.Notably,self-immobilizing fluorescent probes that selectively recognize diseased tissues while improving in situ retention and enrichment enable accurate high-fidelity fluorescence imaging.In this review,we aim to summarize the strategies employed for recent advances in the performance and precision of in vivo fluorescence imaging using self-immobilizing techniques.Lastly,we discuss the prospects and potential challenges associated with selfimmobilizing fluorescent probes to promote further development and application of more delicate fluorescent probes.
基金supported by Scientific research project of Jiangsu Provincial Health Commission(M2021053)Jiangsu Provincial Health Commission Capacity Enhancement Project(ZDXYS202211).
文摘Reactive oxygen species(ROS)are involved in the onset and development of neurodegenerative diseases,such as Alzheimer's disease(AD)and Parkinson's disease(PD).Hydrogen peroxide(H_(2)O_(2)),a key type of ROS,is overexpressed in the early stages of AD and PD and is involved in the disease progression.Assessing H_(2)O_(2) levels in the brain is considered to be a valuable tool for detecting neurodegenerative diseases and exploring their pathogenesis.In this study,we developed two self-immobilizing PET tracers([^(18)F]HYAS and[^(18)F]HYAT)based on a quinone methide(QM)scaffold for non-invasive imaging of H_(2)O_(2) in the brain.Both tracers can respond to H_(2)O_(2) by forming a QM intermediate,which rapidly reacts with nucleophiles.[^(18)F]HYAT,with proper physicochemical properties,is able of crossing the blood-brain barrier.Increased uptake of[^(18)F]HYAT was observed in the brains of mice treated with 1-methyl-4-phenyl-l,2,3,6-tetrahydropyridine(MPTP),indicating that[^(18)F]HYAT is a useful tracer for PET imaging of H_(2)O_(2) in the brain.
