The purpose of this study was to evaluate the antibacterial activity of a modified self-etching primer incorporating chitosan and whether this modification affected the bond strength to radicular dentin.A modified sel...The purpose of this study was to evaluate the antibacterial activity of a modified self-etching primer incorporating chitosan and whether this modification affected the bond strength to radicular dentin.A modified self-etching primer was prepared by adding chitosan solutions at 0.03%,0.06%,0.12% and 0.25%（W/W） to RealSeal selfetching primer.RealSeal primer without chitosan was used as the control.The antibacterial activity of the modified self-etching primer was evaluated using the direct contact test against Enterococcus faecalis.The bonding ability of the RealSeal system to radicular dentin was evaluated using the push-out bond strength test.The modes of failure were examined under a stereomicroscope.Data were analyzed using analysis of variance（ANOVA） and Tukey’s test,with a P-value 〈 0.05 indicating statistical significance.The results showed that the antibacterial properties of the freshly prepared and aged modified self-etching primer incorporating chitosan exhibited potent antibacterial effect against Enterococcus faecalis compared with the unmodified primer.The RealSeal system with the aged modified self-etching primer incorporating chitosan showed no significant differences in the bond strength as compared with the control（P = 0.99）.The findings suggest that modified self-etching primer incorporating chitosan is a promising antibacterial primer which does not adversely affect the bond strength of the RealSeal system to radicular dentin.展开更多
Objective To investigate the effect of multiple coatings of the one-step self-etching adhesive on immediate microtensile bond strength to primary dentin.Methods Twelve caries-free human primary molars were randomly di...Objective To investigate the effect of multiple coatings of the one-step self-etching adhesive on immediate microtensile bond strength to primary dentin.Methods Twelve caries-free human primary molars were randomly divided into 2 groups with 6 teeth each.In group 1,each tooth was hemisected into two halves.One half was assigned to control subgroup 1,which was bonded with a single-step self-etching adhesive according to the manufacturer's instructions;the other half was assigned to experimental subgroup 1 in which the adhesive was applied three times before light curing.In group 2,the teeth were also hemisected into two halves.One half was assigned to control subgroup 2,which was bonded with the single-step self-etching adhesive according to the manufacturer's instructions;the other half was assigned to experimental subgroup 2 in which three layers of adhesive were applied with light curing each successive layer.Microtensile bond strength was immediately tested after specimen preparation.Results When the adhesive was applied three times before light curing,the bond strength of the experimental subgroup 1(n=33,57.49±11.61 MPa) was higher than that of the control subgroup 1(n=31,49.71±11.43 MPa,P<0.05).When using the technique of applying multiple layers of adhesive with light curing each successive layer,no difference of immediate bond strength was observed between the control subgroup 2 and the experimental subgroup 2(P>0.05).Conclusion Multiple coatings of one-step self-etching adhesive can increase the immediate bond strength to primary dentin when using the technique of light-curing after applying three layers of adhesive.展开更多
The effect of saliva contamination on the shear bond strength of orthodontic brackets, at various stages of the bonding procedure using a new self-etch primer was studied. The samples were divided into 4 groups accord...The effect of saliva contamination on the shear bond strength of orthodontic brackets, at various stages of the bonding procedure using a new self-etch primer was studied. The samples were divided into 4 groups according to 4 different enamel surface conditions: Group A: dry; Group B: saliva contamination before priming; Group C: saliva contamination after priming, and Group D: saliva contamination before and after priming. Stainless steel brackets were bonded in each test group with a light-cured composite resin (TransbondXT 3M). The shear bond strength was determined in the first 30 min after bonding. The analysis of variance indicated that the shear bond strengths of the 4 groups were significantly different (F=11.89, P<0.05). Tukey HSD tests indicated that contamination both before and after the application of the acid-etch primer resulted in a significantly lower (=4.6±1.7 MPa) shear bond strength than either the control group (=8.8±1.9 MPa) or the groups where contamination occurred either before (=7.9±2.0 MPa) or after (=6.9±1.5 MPa) the application of the primer. It was concluded that the new acid-etch primer could maintain adequate shear bond strength if contamination occurred either before or after the application of the primer. On the other hand, contamination both before and after the application of the primer could significantly reduce the shear bond strength of orthodontic brackets.展开更多
Sluggish storage kinetics is considered as the main bottleneck of cathode materials for fast-charging aqueous zinc-ion batteries(AZIBs).In this report,we propose a novel in-situ self-etching strategy to unlock the Pal...Sluggish storage kinetics is considered as the main bottleneck of cathode materials for fast-charging aqueous zinc-ion batteries(AZIBs).In this report,we propose a novel in-situ self-etching strategy to unlock the Palm tree-like vanadium oxide/carbon nanofiber membrane(P-VO/C)as a robust freestanding electrode.Comprehensive investigations including the finite element simulation,in-situ X-ray diffraction,and in-situ electrochemical impedance spectroscopy disclosed it an electrochemically induced phase transformation mechanism from VO to layered Zn_(x)V_(2)O_5·nH_(2)O,as well as superior storage kinetics with ultrahigh pseudocapacitive contribution.As demonstrated,such electrode can remain a specific capacity of 285 mA h g^(-1)after 100 cycles at 1 A g^(-1),144.4 mA h g^(-1)after 1500 cycles at 30 A g^(-1),and even 97 mA h g^(-1)after 3000 cycles at 60 A g^(-1),respectively.Unexpectedly,an impressive power density of 78.9 kW kg^(-1)at the super-high current density of 100 A g^(-1)also can be achieved.Such design concept of in-situ self-etching free-standing electrode can provide a brand-new insight into extending the pseudocapacitive storage limit,so as to promote the development of high-power energy storage devices including but not limited to AZIBs.展开更多
Objective To investigate whether multiple coatings can improve the bond durability of one-step self-etching adhesive to primary dentin. Methods Twelve caries-free human primary molars were randomly divided into 2 gro...Objective To investigate whether multiple coatings can improve the bond durability of one-step self-etching adhesive to primary dentin. Methods Twelve caries-free human primary molars were randomly divided into 2 groups. In group 1, each tooth was hemisected into 2 halves. One half was assigned to the control subgroup 1, which was bonded with a commercially available one-step self-etching adhesive according to the manufacturer＇s instruc- tions; the other half was assigned to experimental subgroup 1, in which the adhesive was applied three times before light curing. In group 2, one split half tooth was bonded with a commercially available one-step self-etching adhesive according to the manufacturer＇s instructions; for the other half, three layers of adhesive were applied with each successive layer of light curing. Specimens were stored in 0.9% NaC1 containing 0.02% sodium azide at 37℃ for 18 months and then were subjected to microtensile bond strength test and the fracture mode analysis. Results When the adhesive was applied three times before light curing, the bond strength of the ex- perimental subgroup 1 was significantly higher than that of the control subgroup 1 （47.46__＋13.91 vs. 38.12＋11.21 MPa, P〈O.05）. When using the technique of applying multiple layers of adhesive with each successive layer of light curing, no difference was observed in bond strength between the control subgroup and the experimental subgroup （39.40＋8.87 vs. 40.87-＋9.33 MPa, P〉O.05）. Conclusion Multiple coatings can improve the bond durability of one-step self-etching adhesive to primary dentin when using the technique of light-curing after applying 3 layers of adhesive.展开更多
At present,the polymerase chain reaction(PCR)amplification-based file retrieval method is the mostcommonly used and effective means of DNA file retrieval.The number of orthogonal primers limitsthe number of files that...At present,the polymerase chain reaction(PCR)amplification-based file retrieval method is the mostcommonly used and effective means of DNA file retrieval.The number of orthogonal primers limitsthe number of files that can be accurately accessed,which in turn affects the density in a single oligo poolof digital DNA storage.In this paper,a multi-mode DNA sequence design method based on PCR file retrie-val in a single oligonucleotide pool is proposed for high-capacity DNA data storage.Firstly,by analyzingthe maximum number of orthogonal primers at each predicted primer length,it was found that the rela-tionship between primer length and the maximum available primer number does not increase linearly,and the maximum number of orthogonal primers is on the order of 10^(4).Next,this paper analyzes themaximum address space capacity of DNA sequences with different types of primer binding sites for filemapping.In the case where the capacity of the primer library is R(where R is even),the number ofaddress spaces that can be mapped by the single-primer DNA sequence design scheme proposed in thispaper is four times that of the previous one,and the two-level primer DNA sequence design scheme can reach [R/2·(R/2-1)]^(2)times.Finally,a multi-mode DNA sequence generation method is designed based onthe number of files to be stored in the oligonucleotide pool,in order to meet the requirements of the ran-dom retrieval of target files in an oligonucleotide pool with large-scale file numbers.The performance ofthe primers generated by the orthogonal primer library generator proposed in this paper is verified,andthe average Gibbs free energy of the most stable heterodimer formed between the orthogonal primersproduced is−1 kcal·(mol·L^(−1))^(−1)(1 kcal=4.184 kJ).At the same time,by selectively PCR-amplifying theDNA sequences of the two-level primer binding sites for random access,the target sequence can be accu-rately read with a minimum of 10^(3) reads,when the primer binding site sequences at different positionsare mutually different.This paper provides a pipeline for orthogonal primer library generation and multi-mode mapping schemes between files and primers,which can help achieve precise random access to filesin large-scale DNA oligo pools.展开更多
Despite being ubiquitous in oceans and important in marine biogeochemical cycles,planktonic archaea in the Southern Ocean(SO)remain poorly characterized.Although high-throughput sequencing(HTS)approaches based on 16S ...Despite being ubiquitous in oceans and important in marine biogeochemical cycles,planktonic archaea in the Southern Ocean(SO)remain poorly characterized.Although high-throughput sequencing(HTS)approaches based on 16S ribosomal RNA(rRNA)genes have been used widely to study the diversity and composition of microbial community in natural environments,primer-set selection is critical because of amplicon-sequencing bias during metabarcoding.Here,using surface-seawater samples collected from the area between the South Shetland and South Orkney Islands,Antarctica,we compared primer sets Arch349F/Arch806R,515F-Y/926R,and 524F/Arch958R,which target different 16S rRNA gene hypervariable regions to identify the best one for studying planktonic archaeal communities.With much lower number of bacteria-related sequences,primer set 524F/Arch958R showed higher values of archaeal operational taxonomic units(OTUs)as well as alpha-diversity indices,indicating that this primer set was more specific for detecting archaeal species and could be helpful to obtain more comprehensive information on the archaeal community compositions compared to other two primer sets.Compared with primer set Arch349F/Arch806R revealing four phyla(Halobacteriota,Methanobacteriota,Thermoplasmatota,and Thermoproteota)detected in seawater,additional archaeal phyla were observed by 515F-Y/926R(Asgardarchaeota and Nanoarchaeota)and 524F/Arch958R(Micrarchaeota).In spite of the differences in archaeal community compositions observed among the three investigated primer sets,ammonia-oxidizing(e.g.,Nitrososphaeria)and methane-producing(e.g.,Methanobacteria,Methanomicrobia,and Methanosarcinia)archaea were the main groups detected in the surface seawater,indicating the ecological role of planktonic archaea in carbon and nitrogen cycling in the upper waters of the SO.These results underscore the importance of primer-set selection when studying archaeal community diversity and composition in the Antarctic SO.展开更多
[Objective] The research aimed to design primers that are suitable for detecting H5 and H7 subtypes of avian influenza virus （AIV） ; [Method] DNAStar was used to analyze the homology of the sequences of H5 and H7 su...[Objective] The research aimed to design primers that are suitable for detecting H5 and H7 subtypes of avian influenza virus （AIV） ; [Method] DNAStar was used to analyze the homology of the sequences of H5 and H7 subtypes of AIV accessed in GenBank, and design primers（ by Primer Premier 5.0） on high homologous region of these sequences, and then amplified by RT-PCR. [Result] The multiplex RT-PCR amplification, agarose gel electrophoresis and sequencing results showed that the self-designed primers are successful for detecting AIV. [Conclusion] It is feasible to rapidly diagnose AIV through this method.展开更多
[Objective] This study aimed to screen a set of SSR core primers suitable for purity identification of pepper （Capsicum） hybrids. [Method] DNA fingerprint of 100 pepper hybrids was analyzed using 17 SSR primers. [Re...[Objective] This study aimed to screen a set of SSR core primers suitable for purity identification of pepper （Capsicum） hybrids. [Method] DNA fingerprint of 100 pepper hybrids was analyzed using 17 SSR primers. [Result] According to the polymorphism and heterozygosity, Hpms1-214, Es395 and Hpmsl-5 were determined as three preferred core primers for purity identification of pepper hybrids. By using these three preferred core primers, 97 pepper hybrids （accounting for 97%） had heterozygous band pattern with at least one primer. Es330, Es363, Epms923, Es120 and Es64 were determined as candidate core primers for purity identification of pepper hybrids. Specific primers of 14 varieties were obtained, which could be used to further screen parent-complementary primers of each pepper hybrid. [Con- clusion] This study laid the foundation for constructing standard DNA fingerprints for purity identification of pepper hybrids.展开更多
[Objective] The aim was to explore the special methods for amplification of large-family genes by using primers with high degeneracy.[Method] By using the primers with high degeneracy,conventional PCR,conventional tou...[Objective] The aim was to explore the special methods for amplification of large-family genes by using primers with high degeneracy.[Method] By using the primers with high degeneracy,conventional PCR,conventional touchdown PCR and the optimized abnormal touchdown PCR were respectively carried out to amplify the genomic DNA of Cyprinus carpio.[Result] Only one evident electrophoretic band and a few Sox genes were obtained by using normal PCR;no obvious electrophoretic band but dispersive product was obtained by normal touchdown PCR;ideal result was obtained by the abnormal touchdown PCR that three evident electrophoretic bands and much more Sox genes were amplified.[Conclusion] The research provided theoretical basis for the optimization and selection of PCR amplification conditions of the large-family genes.展开更多
文摘The purpose of this study was to evaluate the antibacterial activity of a modified self-etching primer incorporating chitosan and whether this modification affected the bond strength to radicular dentin.A modified self-etching primer was prepared by adding chitosan solutions at 0.03%,0.06%,0.12% and 0.25%（W/W） to RealSeal selfetching primer.RealSeal primer without chitosan was used as the control.The antibacterial activity of the modified self-etching primer was evaluated using the direct contact test against Enterococcus faecalis.The bonding ability of the RealSeal system to radicular dentin was evaluated using the push-out bond strength test.The modes of failure were examined under a stereomicroscope.Data were analyzed using analysis of variance（ANOVA） and Tukey’s test,with a P-value 〈 0.05 indicating statistical significance.The results showed that the antibacterial properties of the freshly prepared and aged modified self-etching primer incorporating chitosan exhibited potent antibacterial effect against Enterococcus faecalis compared with the unmodified primer.The RealSeal system with the aged modified self-etching primer incorporating chitosan showed no significant differences in the bond strength as compared with the control（P = 0.99）.The findings suggest that modified self-etching primer incorporating chitosan is a promising antibacterial primer which does not adversely affect the bond strength of the RealSeal system to radicular dentin.
文摘Objective To investigate the effect of multiple coatings of the one-step self-etching adhesive on immediate microtensile bond strength to primary dentin.Methods Twelve caries-free human primary molars were randomly divided into 2 groups with 6 teeth each.In group 1,each tooth was hemisected into two halves.One half was assigned to control subgroup 1,which was bonded with a single-step self-etching adhesive according to the manufacturer's instructions;the other half was assigned to experimental subgroup 1 in which the adhesive was applied three times before light curing.In group 2,the teeth were also hemisected into two halves.One half was assigned to control subgroup 2,which was bonded with the single-step self-etching adhesive according to the manufacturer's instructions;the other half was assigned to experimental subgroup 2 in which three layers of adhesive were applied with light curing each successive layer.Microtensile bond strength was immediately tested after specimen preparation.Results When the adhesive was applied three times before light curing,the bond strength of the experimental subgroup 1(n=33,57.49±11.61 MPa) was higher than that of the control subgroup 1(n=31,49.71±11.43 MPa,P<0.05).When using the technique of applying multiple layers of adhesive with light curing each successive layer,no difference of immediate bond strength was observed between the control subgroup 2 and the experimental subgroup 2(P>0.05).Conclusion Multiple coatings of one-step self-etching adhesive can increase the immediate bond strength to primary dentin when using the technique of light-curing after applying three layers of adhesive.
文摘The effect of saliva contamination on the shear bond strength of orthodontic brackets, at various stages of the bonding procedure using a new self-etch primer was studied. The samples were divided into 4 groups according to 4 different enamel surface conditions: Group A: dry; Group B: saliva contamination before priming; Group C: saliva contamination after priming, and Group D: saliva contamination before and after priming. Stainless steel brackets were bonded in each test group with a light-cured composite resin (TransbondXT 3M). The shear bond strength was determined in the first 30 min after bonding. The analysis of variance indicated that the shear bond strengths of the 4 groups were significantly different (F=11.89, P<0.05). Tukey HSD tests indicated that contamination both before and after the application of the acid-etch primer resulted in a significantly lower (=4.6±1.7 MPa) shear bond strength than either the control group (=8.8±1.9 MPa) or the groups where contamination occurred either before (=7.9±2.0 MPa) or after (=6.9±1.5 MPa) the application of the primer. It was concluded that the new acid-etch primer could maintain adequate shear bond strength if contamination occurred either before or after the application of the primer. On the other hand, contamination both before and after the application of the primer could significantly reduce the shear bond strength of orthodontic brackets.
基金financially supported by the Shenzhen Science and Technology Program (JCYJ20200109105805902,JCYJ20220818095805012)the National Natural Science Foundation of China (22208221,22178221,42377487)+2 种基金the Scientific and Technological Plan of Guangdong Province (2019B090905005,2019B090911004)the Natural Science Foundation of Guangdong Province (2021A1515110751)the Guangdong Basic and Applied Basic Research Foundation (2022A1515110477,2021B1515120004)。
文摘Sluggish storage kinetics is considered as the main bottleneck of cathode materials for fast-charging aqueous zinc-ion batteries(AZIBs).In this report,we propose a novel in-situ self-etching strategy to unlock the Palm tree-like vanadium oxide/carbon nanofiber membrane(P-VO/C)as a robust freestanding electrode.Comprehensive investigations including the finite element simulation,in-situ X-ray diffraction,and in-situ electrochemical impedance spectroscopy disclosed it an electrochemically induced phase transformation mechanism from VO to layered Zn_(x)V_(2)O_5·nH_(2)O,as well as superior storage kinetics with ultrahigh pseudocapacitive contribution.As demonstrated,such electrode can remain a specific capacity of 285 mA h g^(-1)after 100 cycles at 1 A g^(-1),144.4 mA h g^(-1)after 1500 cycles at 30 A g^(-1),and even 97 mA h g^(-1)after 3000 cycles at 60 A g^(-1),respectively.Unexpectedly,an impressive power density of 78.9 kW kg^(-1)at the super-high current density of 100 A g^(-1)also can be achieved.Such design concept of in-situ self-etching free-standing electrode can provide a brand-new insight into extending the pseudocapacitive storage limit,so as to promote the development of high-power energy storage devices including but not limited to AZIBs.
文摘Objective To investigate whether multiple coatings can improve the bond durability of one-step self-etching adhesive to primary dentin. Methods Twelve caries-free human primary molars were randomly divided into 2 groups. In group 1, each tooth was hemisected into 2 halves. One half was assigned to the control subgroup 1, which was bonded with a commercially available one-step self-etching adhesive according to the manufacturer＇s instruc- tions; the other half was assigned to experimental subgroup 1, in which the adhesive was applied three times before light curing. In group 2, one split half tooth was bonded with a commercially available one-step self-etching adhesive according to the manufacturer＇s instructions; for the other half, three layers of adhesive were applied with each successive layer of light curing. Specimens were stored in 0.9% NaC1 containing 0.02% sodium azide at 37℃ for 18 months and then were subjected to microtensile bond strength test and the fracture mode analysis. Results When the adhesive was applied three times before light curing, the bond strength of the ex- perimental subgroup 1 was significantly higher than that of the control subgroup 1 （47.46__＋13.91 vs. 38.12＋11.21 MPa, P〈O.05）. When using the technique of applying multiple layers of adhesive with each successive layer of light curing, no difference was observed in bond strength between the control subgroup and the experimental subgroup （39.40＋8.87 vs. 40.87-＋9.33 MPa, P〉O.05）. Conclusion Multiple coatings can improve the bond durability of one-step self-etching adhesive to primary dentin when using the technique of light-curing after applying 3 layers of adhesive.
基金supported by the fund from Tianjin Municipal Science and Technology Bureau(22JCYBJC01390).
文摘At present,the polymerase chain reaction(PCR)amplification-based file retrieval method is the mostcommonly used and effective means of DNA file retrieval.The number of orthogonal primers limitsthe number of files that can be accurately accessed,which in turn affects the density in a single oligo poolof digital DNA storage.In this paper,a multi-mode DNA sequence design method based on PCR file retrie-val in a single oligonucleotide pool is proposed for high-capacity DNA data storage.Firstly,by analyzingthe maximum number of orthogonal primers at each predicted primer length,it was found that the rela-tionship between primer length and the maximum available primer number does not increase linearly,and the maximum number of orthogonal primers is on the order of 10^(4).Next,this paper analyzes themaximum address space capacity of DNA sequences with different types of primer binding sites for filemapping.In the case where the capacity of the primer library is R(where R is even),the number ofaddress spaces that can be mapped by the single-primer DNA sequence design scheme proposed in thispaper is four times that of the previous one,and the two-level primer DNA sequence design scheme can reach [R/2·(R/2-1)]^(2)times.Finally,a multi-mode DNA sequence generation method is designed based onthe number of files to be stored in the oligonucleotide pool,in order to meet the requirements of the ran-dom retrieval of target files in an oligonucleotide pool with large-scale file numbers.The performance ofthe primers generated by the orthogonal primer library generator proposed in this paper is verified,andthe average Gibbs free energy of the most stable heterodimer formed between the orthogonal primersproduced is−1 kcal·(mol·L^(−1))^(−1)(1 kcal=4.184 kJ).At the same time,by selectively PCR-amplifying theDNA sequences of the two-level primer binding sites for random access,the target sequence can be accu-rately read with a minimum of 10^(3) reads,when the primer binding site sequences at different positionsare mutually different.This paper provides a pipeline for orthogonal primer library generation and multi-mode mapping schemes between files and primers,which can help achieve precise random access to filesin large-scale DNA oligo pools.
基金The National Key Research and Development Program of China under contract No.2022YFC2807501.
文摘Despite being ubiquitous in oceans and important in marine biogeochemical cycles,planktonic archaea in the Southern Ocean(SO)remain poorly characterized.Although high-throughput sequencing(HTS)approaches based on 16S ribosomal RNA(rRNA)genes have been used widely to study the diversity and composition of microbial community in natural environments,primer-set selection is critical because of amplicon-sequencing bias during metabarcoding.Here,using surface-seawater samples collected from the area between the South Shetland and South Orkney Islands,Antarctica,we compared primer sets Arch349F/Arch806R,515F-Y/926R,and 524F/Arch958R,which target different 16S rRNA gene hypervariable regions to identify the best one for studying planktonic archaeal communities.With much lower number of bacteria-related sequences,primer set 524F/Arch958R showed higher values of archaeal operational taxonomic units(OTUs)as well as alpha-diversity indices,indicating that this primer set was more specific for detecting archaeal species and could be helpful to obtain more comprehensive information on the archaeal community compositions compared to other two primer sets.Compared with primer set Arch349F/Arch806R revealing four phyla(Halobacteriota,Methanobacteriota,Thermoplasmatota,and Thermoproteota)detected in seawater,additional archaeal phyla were observed by 515F-Y/926R(Asgardarchaeota and Nanoarchaeota)and 524F/Arch958R(Micrarchaeota).In spite of the differences in archaeal community compositions observed among the three investigated primer sets,ammonia-oxidizing(e.g.,Nitrososphaeria)and methane-producing(e.g.,Methanobacteria,Methanomicrobia,and Methanosarcinia)archaea were the main groups detected in the surface seawater,indicating the ecological role of planktonic archaea in carbon and nitrogen cycling in the upper waters of the SO.These results underscore the importance of primer-set selection when studying archaeal community diversity and composition in the Antarctic SO.
基金Supported by Important Project of Jinlin Provincial Science and Technology Department(20065020)~~
文摘[Objective] The research aimed to design primers that are suitable for detecting H5 and H7 subtypes of avian influenza virus （AIV） ; [Method] DNAStar was used to analyze the homology of the sequences of H5 and H7 subtypes of AIV accessed in GenBank, and design primers（ by Primer Premier 5.0） on high homologous region of these sequences, and then amplified by RT-PCR. [Result] The multiplex RT-PCR amplification, agarose gel electrophoresis and sequencing results showed that the self-designed primers are successful for detecting AIV. [Conclusion] It is feasible to rapidly diagnose AIV through this method.
基金Supported by Excellent Team Training Program of Yunnan Academy of Agriculture Sciences(YAAS2014YY002)~~
文摘[Objective] This study aimed to screen a set of SSR core primers suitable for purity identification of pepper （Capsicum） hybrids. [Method] DNA fingerprint of 100 pepper hybrids was analyzed using 17 SSR primers. [Result] According to the polymorphism and heterozygosity, Hpms1-214, Es395 and Hpmsl-5 were determined as three preferred core primers for purity identification of pepper hybrids. By using these three preferred core primers, 97 pepper hybrids （accounting for 97%） had heterozygous band pattern with at least one primer. Es330, Es363, Epms923, Es120 and Es64 were determined as candidate core primers for purity identification of pepper hybrids. Specific primers of 14 varieties were obtained, which could be used to further screen parent-complementary primers of each pepper hybrid. [Con- clusion] This study laid the foundation for constructing standard DNA fingerprints for purity identification of pepper hybrids.
文摘[Objective] The aim was to explore the special methods for amplification of large-family genes by using primers with high degeneracy.[Method] By using the primers with high degeneracy,conventional PCR,conventional touchdown PCR and the optimized abnormal touchdown PCR were respectively carried out to amplify the genomic DNA of Cyprinus carpio.[Result] Only one evident electrophoretic band and a few Sox genes were obtained by using normal PCR;no obvious electrophoretic band but dispersive product was obtained by normal touchdown PCR;ideal result was obtained by the abnormal touchdown PCR that three evident electrophoretic bands and much more Sox genes were amplified.[Conclusion] The research provided theoretical basis for the optimization and selection of PCR amplification conditions of the large-family genes.
