To provide reference for selenium resource exploitation of soil,many selenium-tolerant strains were isolated from selenium-rich soil in Guangxi. The dilution spread plate method and selenium-added culture method were ...To provide reference for selenium resource exploitation of soil,many selenium-tolerant strains were isolated from selenium-rich soil in Guangxi. The dilution spread plate method and selenium-added culture method were used to screen the selenium-tolerant strains from the soils which were sampled from the main Selenium-rich areas such as Yongfu,Bama,Yulin Hanshan,Guiping and Tengxian. The results showed that 8 strains with high selenium tolerance were obtained,which could tolerate the selenium concentration above 10 000 μg/m L in solid medium. Among the 8 strains,YLB1-33 showed the highest selenium tolerance,and it could still grow weakly in the solid medium with selenium concentration of 29 000 μg/m L. The sequencing of 16 S rD NA and phylogenetic analysis showed that YLB1-6 was identified as Bacillus cereus,BMB2-1 and TXB1-8 were identified as Bacillus pumilus,GPB2-5 was identified as Bacillus thuringiensis,YLB1-26 and YLB1-33 were identified as Bacillus licheniformis,and YLB1-2 and YFB1-8 were identified as Serratia marcescens. The finding of selenium-tolerance strains had potential application value on promoting the utilization of selenium soil resources and the development of selenium-rich agricultural products in Guangxi.展开更多
Selenium protects cells from oxidative damage and reduces lipopolysaccharide （LPS）-induced expression of inflammatory cytokines. Because inflammatory cytokines induce growth depression, we hypothesized that selenium...Selenium protects cells from oxidative damage and reduces lipopolysaccharide （LPS）-induced expression of inflammatory cytokines. Because inflammatory cytokines induce growth depression, we hypothesized that selenium-rich yeast （SeY） would inhibit growth depression caused by LPS. Twenty Meishan weanling pigs [28 （s.d. 2 d）, 7.6 （s.d. 0.3 kg） body weight （BW）] were used in a 2×2 factorial design （n=5）. The main factors were dietary Se sources [selenium-rich yeast （SeY） vs. sodium selenite （SSe）] with supplement level at 0.3 mg Se/kg, and immunological stress （LPS vs. saline）. The experiment lasted for 28 d. On d 14 and d 21, half of pigs in each dietary treatment were intramuscularly injected with either 50 μg/kg BW LPS or an equivalent amount of sterile saline. Blood samples were collected at 3 h post-injection. There were no interactions between dietary Se sources and immunological stress on growth performance. LPS depressed average daily gain （ADG） （d 14-21, P〈0.05; d 21-28, P〈 0.01）, and elevated feed to gain ratio （F/G） （d 21-28, P〈0.05）, while SeY elevated ADG （d 0-14, P〈0.05; d 21-28 and d 0-28, P〈0.01）, and decreased F/G （P〈0.01）. Interactions were observed on interleukin-1β（IL-1β） and interleukin-6 （IL-6） from serum samples of d 14 and d 21 （P〈0.01）, as well as superoxide dismutase （SOD） and insulin like growth factor （IGF-1） of d 21 （P〈0.05）. In conclusion, SeY relieved the growth depression of piglets immunologically stressed by LPS, which maybe resulted from the decreased externalization of inflammatory cytokines and improved antioxidant status.展开更多
This paper presents an overview of the agricultural background of Shatian Town,and the implementation process of selenium-rich agriculture,including scientific selection,technical training,demonstration projects,and m...This paper presents an overview of the agricultural background of Shatian Town,and the implementation process of selenium-rich agriculture,including scientific selection,technical training,demonstration projects,and market expansion.Additionally,the paper discusses the practical outcomes achieved,including improvements in economic benefits and enhancements to the ecological environment.Meantime,it identifies existing challenges,such as technical bottlenecks and insufficient funding,and proposes measures for improvement,including the strengthening of scientific and technological innovation and the enhancement of market mechanisms.展开更多
【目的】研究不同添加剂对天然富硒(Se)区镉(Cd)污染土壤硒、镉有效性以及玉米生长和地上部硒、镉含量的影响,旨在为天然富硒区镉污染耕地的安全利用提供理论依据。【方法】以玉米正大12号为供试品种,选用凹凸棒土、硅肥、表面活性剂(...【目的】研究不同添加剂对天然富硒(Se)区镉(Cd)污染土壤硒、镉有效性以及玉米生长和地上部硒、镉含量的影响,旨在为天然富硒区镉污染耕地的安全利用提供理论依据。【方法】以玉米正大12号为供试品种,选用凹凸棒土、硅肥、表面活性剂(鼠李糖脂与皂素)作为添加剂,采用盆栽试验,共设置空白对照(CK)、添加1%凹凸棒土(A1)、添加3%凹凸棒土(A2)、添加5%凹凸棒土(A3)、叶面喷施400 mL 1.6 g/L硅肥(B)、添加200 mL 20 g/L鼠李糖脂(C1)、添加200 mL 20 g/L皂素(C2)、3%凹凸棒土+200 mL 20 g/L鼠李糖脂+叶面喷施400 mL 1.6 g/L硅肥(D1)、3%凹凸棒土+200 mL 20 g/L皂素+叶面喷施400 mL 1.6 g/L硅肥(D2)9个处理,分析不同处理对土壤pH、阳离子交换量(CEC)、全Cd、有效态Cd、有效态Se含量以及玉米生长和地上部Cd、Se含量的影响。【结果】与CK相比,A2、B、D1、D2处理均显著提高了玉米株高,增加了玉米根长。除了B处理,其余添加剂处理土壤pH较CK显著提高。与CK相比,A1、B、C1、C2处理土壤CEC无显著变化,而A2、A3、D1、D2处理土壤CEC分别显著提高了20.67%,27.18%,23.30%和26.16%。各处理土壤全Cd含量无显著差异。与CK相比,除了B处理,其余添加剂处理土壤有效态Cd含量均显著降低,其中A3、D1、D2处理降幅较大。A2、A3、D1、D2处理玉米地上部Cd含量与CK相比均显著降低,其中A3、D1、D2处理降幅最大,分别为31.20%,31.47%,30.25%。各处理对土壤有效态Se以及玉米地上部Se含量无显著影响。【结论】不同添加剂对天然富Se区Cd污染土壤Cd有效性以及玉米生长和地上部Cd含量有明显的影响,其中3%凹凸棒土与表面活性剂(鼠李糖脂或皂素)和硅肥联合施用能有效降低土壤有效态Cd及玉米地上部Cd含量,促进玉米生长。展开更多
基金Supported by the Program for the Scientific Research and Technology Development in Guangxi(Guikehe 415104001-22)the Science and Technology Planning Project of Guangxi(Guikegong 1598006-5-13)+2 种基金the Experiment Station for Selenium Featured Crops in Guangxi(Gui TS2016011)the Fund for Science and Technology Development and Scientific Research of Guangxi Academy of Agricultural Sciences(Guinongke 2015JM23,2017JM01,2017JM03)the Innovation-Driven Development Special Fund Project of Guangxi(Guike AA17202019-1&Guike AA17202019-4)
文摘To provide reference for selenium resource exploitation of soil,many selenium-tolerant strains were isolated from selenium-rich soil in Guangxi. The dilution spread plate method and selenium-added culture method were used to screen the selenium-tolerant strains from the soils which were sampled from the main Selenium-rich areas such as Yongfu,Bama,Yulin Hanshan,Guiping and Tengxian. The results showed that 8 strains with high selenium tolerance were obtained,which could tolerate the selenium concentration above 10 000 μg/m L in solid medium. Among the 8 strains,YLB1-33 showed the highest selenium tolerance,and it could still grow weakly in the solid medium with selenium concentration of 29 000 μg/m L. The sequencing of 16 S rD NA and phylogenetic analysis showed that YLB1-6 was identified as Bacillus cereus,BMB2-1 and TXB1-8 were identified as Bacillus pumilus,GPB2-5 was identified as Bacillus thuringiensis,YLB1-26 and YLB1-33 were identified as Bacillus licheniformis,and YLB1-2 and YFB1-8 were identified as Serratia marcescens. The finding of selenium-tolerance strains had potential application value on promoting the utilization of selenium soil resources and the development of selenium-rich agricultural products in Guangxi.
文摘Selenium protects cells from oxidative damage and reduces lipopolysaccharide （LPS）-induced expression of inflammatory cytokines. Because inflammatory cytokines induce growth depression, we hypothesized that selenium-rich yeast （SeY） would inhibit growth depression caused by LPS. Twenty Meishan weanling pigs [28 （s.d. 2 d）, 7.6 （s.d. 0.3 kg） body weight （BW）] were used in a 2×2 factorial design （n=5）. The main factors were dietary Se sources [selenium-rich yeast （SeY） vs. sodium selenite （SSe）] with supplement level at 0.3 mg Se/kg, and immunological stress （LPS vs. saline）. The experiment lasted for 28 d. On d 14 and d 21, half of pigs in each dietary treatment were intramuscularly injected with either 50 μg/kg BW LPS or an equivalent amount of sterile saline. Blood samples were collected at 3 h post-injection. There were no interactions between dietary Se sources and immunological stress on growth performance. LPS depressed average daily gain （ADG） （d 14-21, P〈0.05; d 21-28, P〈 0.01）, and elevated feed to gain ratio （F/G） （d 21-28, P〈0.05）, while SeY elevated ADG （d 0-14, P〈0.05; d 21-28 and d 0-28, P〈0.01）, and decreased F/G （P〈0.01）. Interactions were observed on interleukin-1β（IL-1β） and interleukin-6 （IL-6） from serum samples of d 14 and d 21 （P〈0.01）, as well as superoxide dismutase （SOD） and insulin like growth factor （IGF-1） of d 21 （P〈0.05）. In conclusion, SeY relieved the growth depression of piglets immunologically stressed by LPS, which maybe resulted from the decreased externalization of inflammatory cytokines and improved antioxidant status.
基金Supported by Huiyang District Rural Science and Technology Specialists Team Stationed in Shatian Town for Rural Revitalization in Guangdong Province.
文摘This paper presents an overview of the agricultural background of Shatian Town,and the implementation process of selenium-rich agriculture,including scientific selection,technical training,demonstration projects,and market expansion.Additionally,the paper discusses the practical outcomes achieved,including improvements in economic benefits and enhancements to the ecological environment.Meantime,it identifies existing challenges,such as technical bottlenecks and insufficient funding,and proposes measures for improvement,including the strengthening of scientific and technological innovation and the enhancement of market mechanisms.
文摘【目的】研究不同添加剂对天然富硒(Se)区镉(Cd)污染土壤硒、镉有效性以及玉米生长和地上部硒、镉含量的影响,旨在为天然富硒区镉污染耕地的安全利用提供理论依据。【方法】以玉米正大12号为供试品种,选用凹凸棒土、硅肥、表面活性剂(鼠李糖脂与皂素)作为添加剂,采用盆栽试验,共设置空白对照(CK)、添加1%凹凸棒土(A1)、添加3%凹凸棒土(A2)、添加5%凹凸棒土(A3)、叶面喷施400 mL 1.6 g/L硅肥(B)、添加200 mL 20 g/L鼠李糖脂(C1)、添加200 mL 20 g/L皂素(C2)、3%凹凸棒土+200 mL 20 g/L鼠李糖脂+叶面喷施400 mL 1.6 g/L硅肥(D1)、3%凹凸棒土+200 mL 20 g/L皂素+叶面喷施400 mL 1.6 g/L硅肥(D2)9个处理,分析不同处理对土壤pH、阳离子交换量(CEC)、全Cd、有效态Cd、有效态Se含量以及玉米生长和地上部Cd、Se含量的影响。【结果】与CK相比,A2、B、D1、D2处理均显著提高了玉米株高,增加了玉米根长。除了B处理,其余添加剂处理土壤pH较CK显著提高。与CK相比,A1、B、C1、C2处理土壤CEC无显著变化,而A2、A3、D1、D2处理土壤CEC分别显著提高了20.67%,27.18%,23.30%和26.16%。各处理土壤全Cd含量无显著差异。与CK相比,除了B处理,其余添加剂处理土壤有效态Cd含量均显著降低,其中A3、D1、D2处理降幅较大。A2、A3、D1、D2处理玉米地上部Cd含量与CK相比均显著降低,其中A3、D1、D2处理降幅最大,分别为31.20%,31.47%,30.25%。各处理对土壤有效态Se以及玉米地上部Se含量无显著影响。【结论】不同添加剂对天然富Se区Cd污染土壤Cd有效性以及玉米生长和地上部Cd含量有明显的影响,其中3%凹凸棒土与表面活性剂(鼠李糖脂或皂素)和硅肥联合施用能有效降低土壤有效态Cd及玉米地上部Cd含量,促进玉米生长。
