Background Organic selenium(Se)has gained recognition in poultry nutrition as a feed additive to boost production and Se deposition in eggs and tissues,owing to its high bioavailability,efficient tissue accumulation a...Background Organic selenium(Se)has gained recognition in poultry nutrition as a feed additive to boost production and Se deposition in eggs and tissues,owing to its high bioavailability,efficient tissue accumulation and minimal toxicity.Selenium-enriched yeast(SeY)is a well-established source,while selenium-enriched lactobacilli(SeL),a newer alternative,offers the added benefits of probiotics.This study examined the effects of SeY and SeL on egg quality,antioxidant capacity,Se deposition,and gut health in laying hens.After a two-week pre-treatment with a Sedeficient diet(SeD),450 Hy-Line Brown laying hens(30-week-old)were assigned into five dietary groups with six replicates of 15 hens each.The groups included a SeD,SeD supplemented with 1.5 mg Se/kg from SeY(SeY15),or 1.5,3.0,and 6.0 mg Se/kg from SeL(SeL15,SeL30,SeL60).The feeding trial lasted for 12 weeks.Results SeY15 and SeL15 improved the feed-to-egg ratio(P<0.05)in the latter stages.Haugh units were significantly increased(P<0.05)in the SeY15 and SeL30 groups,while darker yolk color(P<0.05)was observed in the SeY15,SeL15,and SeL60 groups.All Se-supplemented diets increased Se content in whole eggs,albumen,and yolk(P<0.05),while SeL groups showed a dose-dependent effect.Antioxidant enzyme activities increased,and MDA content decreased in the serum(P<0.05),with SeY15 showing the highest GSH-Px levels(P<0.05).SeL60 increased serum alkaline phosphatase and aspartate transaminase,and distorted the liver architecture(P<0.05).Se-diets reduced concentrations of reactive oxygen species(ROS)in the ileum and liver(P<0.05).SeL15 improved the ileal villus height-tocrypt depth ratio(P<0.05).SeY15 and/or SeL15 up-regulated TXNRD1 and SEPHS1 mRNA while down-regulating SCLY expression in the liver.SeY15 altered ileal microbiota by increasing both beneficial and pathogenic bacteria,whereas SeL15 predominantly boosted beneficial bacteria.Conclusion SeL integrates the antioxidant properties of organic Se with the probiotic benefits on gut health,resulting in a performance-enhancing effect comparable to that of SeY.However,high SeL level(6.0 mg Se/kg)compromised productivity and metabolic functions while enhancing Se deposition.展开更多
The objective of this study was to verify the protective effect of Bifidobacterium longum(BL) and the synergistical effect of Selenium and BL on alcohol plus high fat diet(HFD) induced hepatic injury in mice. We also ...The objective of this study was to verify the protective effect of Bifidobacterium longum(BL) and the synergistical effect of Selenium and BL on alcohol plus high fat diet(HFD) induced hepatic injury in mice. We also want to explore the mechanism of Selenium-enriched Bifidobacterium longum(Se BL). C57 BL/6 mice were treated with alcohol plus HFD with or without different dosage of BL or Se BL for 4 weeks. Serum levels of ALT, AST, TC, TG, LDL-C, HDL-C, FFAs, TNF-α, IL-6 and IL-1β, hepatic MDA level, SOD activity, the m RNA levels of AMPK, PPAR-α and SREBP1 were invested. Se BL inhibited lipid accumulation in hepatocytes;reduced serum AST and ALT levels;improved dyslipidemia;decreased serum FFAs, TC, TG and LDL-C levels. Se BL also inhibited alcohol plus HFD-induced hepatocyte oxidative stress through decrease in hepatic MDA levels and increase in SOD activity. Se BL also regulated lipid metabolism related genes such as AMPK, PPAR-α and SREBP1. Although BL had similar effect as Se BL, Se BL is more effective than BL. Se BL protected mice from alcohol plus HFD-induced hepatic injury in mice because of its inhibitory effect on hepatocellular oxidative stress, lipogenesis and inflammation. Selenium enhanced the protective effect of BL.展开更多
High-salt diet is well recognized as a risk factor for hypertension,and dietary intervention plays a critical role in the prevention of hypertension.The current study investigated the effects of selenium-enriched gree...High-salt diet is well recognized as a risk factor for hypertension,and dietary intervention plays a critical role in the prevention of hypertension.The current study investigated the effects of selenium-enriched green tea(Se-GT)and ordinary green tea(GT)on prevention of hypertension of rats induced by high-salt diet,as well as their potential regulatory and mechanism.Our results showed that GT and Se-GT supplementations significantly prevented the increase of blood pressure(BP),activated the phosphoinosmde-3-kinase/protein kinase B(PI3K/Akt)signaling pathway,and regulated the gene expression related to BP,as well as improved the tissue damage like heart,liver,and kidneys.Besides,the key parameters associated with oxidative stress,inflammation and endothelial dysfunction were also altered by GT and Se-GT treatments.Importantly,GT or Se-GT administration adjusted the diversity and composition of the intestinal flora.Moreover,GT and Se-GT supplementations increased the abundance of beneficial bacteria and reduced the abundance of harmful or conditional pathogenic bacteria.More specifically,GT intake specifically and significantly enriched the relative abundance of Paraprevotella and Bacteroides,whereas Se-GT was characterized by specific and significant enrichment for Allobaculum and Bifidobacterium.Our results proved that dietary supplement of GT and Se-GT remarkably improved the vascular functions and effectively prevented tissue damage by regulation of intestinal flora,and thus preventing hypertension induced by high-salt diet.展开更多
As an important symbol of agricultural and sideline products in Ankang City,selenium-enriched tea affects the development of regional agricultural and sideline industries.In this paper,the development status and probl...As an important symbol of agricultural and sideline products in Ankang City,selenium-enriched tea affects the development of regional agricultural and sideline industries.In this paper,the development status and problems of the selenium-enriched tea products of Ankang were analyzed in terms of capital,technology,management and sales mode.This research is of positive significance to the development of selenium-enriched products and other agricultural and sideline products of health value,and plays a very important role in promoting the cultivation of village-level characteristic industries and the effective realization of targeted poverty alleviation.展开更多
[Objectives] The aims were to optimize the extraction process of selenoproteins from selenium-enriched rice in Guangxi and provide references for the intensive processing and comprehensive utilization of selenium prot...[Objectives] The aims were to optimize the extraction process of selenoproteins from selenium-enriched rice in Guangxi and provide references for the intensive processing and comprehensive utilization of selenium protein resources. [Methods]Selenium-enriched rice was used as materials to extract selenoproteins by phosphate buffer extraction method and to optimize the extraction process of selenoproteins by using the orthogonal experiment. Proteins and selenium content was measured by Coomassie Brilliant Blue G-250 reagent and AFS（ atomic fluorescence spectrometry） respectively. [Results] The most significant factor affecting extraction of rice Selenoproteins was extraction NaO H concentration,followed by the ratio of solid-liquid,temperature and then extraction time. The optimum extraction conditions of selenoproteins from rice were extraction temperature of 50 ℃,NaO H concentration of 0. 14 mol/L,extraction time of 5 h,and solid-liquid ratio of 1∶ 30. [Conclusions]The alkali extraction process optimized by orthogonal test could effectively improve the extraction rate of selenoproteins,and the optimized process parameters could be popularized and applied in practical production.展开更多
Selenium is an essential trace element for human health.Eating selenium-enriched eggs is conducive to easily and effectively solving the problem of selenium deficiency.In the production process of selenium-enriched eg...Selenium is an essential trace element for human health.Eating selenium-enriched eggs is conducive to easily and effectively solving the problem of selenium deficiency.In the production process of selenium-enriched eggs,different selenium sources,additive doses,feeding time,and the addition of other vitamins,trace elements,and methionine in the diet will influence the deposition of selenium in eggs.Through this review,it is intended to provide a reference for the practical,safe and economical production of selenium-enriched eggs.展开更多
Taking Scientific Outlook on Development as the guiding principle,this paper introduced the principles of innovation,coordination,green,openness and sharing. Based on gifted advantages of selenium-enriched soil and se...Taking Scientific Outlook on Development as the guiding principle,this paper introduced the principles of innovation,coordination,green,openness and sharing. Based on gifted advantages of selenium-enriched soil and selenium-enriched industry development in Guangxi,it discussed current practice,stressed green,ecological,characteristic demonstration,transformation and upgrading,and targeted poverty alleviation. Finally,it came up with perspective,practical,and operational strategies and recommendations for development of selenium-enriched industry,to provide reference for sustainable development of selenium-enriched functional industry in Guangxi.展开更多
In order to improve the added value of the main vegetable products in Qinghai Province,we researched the effect of soil selenium content on vegetable quality in selenium-enriched area of Qinghai. Through the analysis ...In order to improve the added value of the main vegetable products in Qinghai Province,we researched the effect of soil selenium content on vegetable quality in selenium-enriched area of Qinghai. Through the analysis of Qinghai selenium-enriched soil selenium content and5 kinds of main vegetables corresponds to the total selenium,part of the mineral elements and soluble sugar and VC content,the effects of soil selenium levels on selenium absorption and nourishment quality of vegetables were researched. The results showed that he Qinghai selenium-enriched soil selenium content in the range of 100. 00-563. 00 μg/kg,among them,the soil samples with sufficient selenium and rich selenium accounted for 76. 53% and 18. 88%,respectively. Soil selenium content of garlic sampling area was relatively high,and soil selenium content of sugar beet sampling area was relatively low. Vegetable selenium content was in the range of 11. 00-340. 94 μg/kg,the average content of total selenium content of garlic was up to 170. 40 μg/kg,and the average content of the total selenium content of radish is 73. 00 μg/kg.90. 63% of the vegetables in the region reached the level of sufficient selenium,and 70. 31% reached the level of selenium enrichment. The average content of Ca and Mg was higher than the national average. There was no significant correlation between selenium content of soil and vegetable and nutritional quality.展开更多
To investigate the in vitro digestion and fermentation properties of soybean oligosaccharides(SBOS)extracted from defatted soybean meal,the changes in monosaccharide composition and molecular mass were analyzed.Subseq...To investigate the in vitro digestion and fermentation properties of soybean oligosaccharides(SBOS)extracted from defatted soybean meal,the changes in monosaccharide composition and molecular mass were analyzed.Subsequently,the effect of SBOS on microbial community structure and metabolites was studied by 16S rRNA gene sequencing and untargeted metabolomics based on liquid chromatography-mass spectrometry.Results showed that SBOS was not easily enzymolyzed during simulated digestion and could reach the large intestine through the digestive system.The significant decrease in the molecular mass of SBOS after in vitro fermentation indicated its utilization by the gut microbiota,which increased the contents of short-chain fatty acids and lactic acid,thereby reducing the pH of the fermentation broth.Moreover,the core community was found to consist of Blautia,Lactobacillaceae,and Pediococcus.SBOS up-regulated beneficial differential metabolites such as myo-inositol,lactose,and glucose,which were closely related to galactose,amino sugar,and nucleotide sugar metabolism.This study will provide a reference for exploring the relationship between the gut microbiota and the metabolites of SBOS,and provide a basis for the development and application of SBOS as an ingredient for functional products.展开更多
Dense cropping increases crop yield but intensifies resource competition,which reduces single plant yield and limits potential yield growth.Optimizing canopy spacing could enhance resource utilization,support crop mor...Dense cropping increases crop yield but intensifies resource competition,which reduces single plant yield and limits potential yield growth.Optimizing canopy spacing could enhance resource utilization,support crop morphological development and increase yield.Here,a three-year study was performed to verify the feasibility of adjusting row spacing to further enhance yield in densely planted soybeans.Of three row-spacing configurations(40-40,20-40,and 20-60 cm)and two planting densities(normal 180,000 plants ha 1 and high 270,000 plants ha 1).The differences in canopy structure,plant morphological development,photosynthetic capacity and their impact on yield were analyzed.Row spacing configurations have a significant effect on canopy transmittance(CT).The 20-60 cm row spacing configuration increased CT and creates a favorable canopy light environment,in which plant height is reduced,while branching is promoted.This approach reduces plant competition,optimizes the developments of leaf area per plant,specific leaf area,leaf area development rate,leaf area duration and photosynthetic physiological indices(F_(v)/F_(m),ETR,P_(n)).The significant increase of 11.9%-34.2%in canopy apparent photosynthesis(CAP)is attributed to the significant optimization of plant growth and photosynthetic physiology through CT,an important contributing factor to yield increases.The yield in the 20-60 cm treatment is 4.0%higher than in equidistant planting under normal planting density,but 5.9%under high density,primarily driven by CAP and pod number.These findings suggest that suitable row spacing configurations optimize the light environment for plants,promote source-sink transformation in soybeans,and further improve yield.In practice,a 20-60 cm row spacing configuration could be employed for high-density soybean planting to achieve a more substantial yield gain.展开更多
Plant specialized metabolites are commonly stored in glycosylated forms within plant cells,with their homeostasis regulated by glycosyltransferases andβ-glucosidases(BGLUs,also known asβ-glucoside hydrolases(E.C.3.2...Plant specialized metabolites are commonly stored in glycosylated forms within plant cells,with their homeostasis regulated by glycosyltransferases andβ-glucosidases(BGLUs,also known asβ-glucoside hydrolases(E.C.3.2.1.21)).Soyasaponins,the predominant triterpenoid compounds(C30)in soybean seeds,contain two sugar moieties attached at the C3 and C22 positions.While glycosyltransferases involved in soyasaponin biosynthesis have been well characterized,the role of BGLUs in soyasaponin homeostasis remains unclear.In this study,we identified GmSSBG1(Soyasaponinβ-glucosidase1;Glyma.07G258700)as a candidate gene potentially involved in soyasaponin homeostasis through gene to gene co-expression analysis.Biochemical assays demonstrated that GmSSBG1 specifically hydrolyzes arabinose residues at the C22 position of A0-and B0-series soyasaponins.Loss-of-function mutations in GmSSBG1 led to a significant accumulation of A0-and B0-series soyasaponins in mutant seeds,which correlated with a pronounced decrease in resistance to the soybean pod borer(Leguminivora glycinivorella).Our findings provide critical insights into the regulatory mechanisms underlying soyasaponin homeostasis and lay a theoretical foundation for molecular breeding strategies aimed at developing soybean lines with enhanced resistance to soybean pod borer,even to other insect pests.展开更多
Soybean meal(SBM)prepared by soybean crushing is the most popular protein source in the poultry and livestock industries(Cai et al.,2015)due to its economic manufacture,high protein content,and good nutritional value....Soybean meal(SBM)prepared by soybean crushing is the most popular protein source in the poultry and livestock industries(Cai et al.,2015)due to its economic manufacture,high protein content,and good nutritional value.Despite these benefits,SBM contains various antigen proteins such as glycinin andβ-conglycinin,which account for approximately 70%of the total proteins of the SBM and reduce digestibility and damage intestinal function(Peng et al.,2018).展开更多
Maize/soybean intercropping systems are commonly used in developing countries,but few studies have been performed to elucidate the differences in nutrient efficiency and rhizosphere microbiome,especially when maize is...Maize/soybean intercropping systems are commonly used in developing countries,but few studies have been performed to elucidate the differences in nutrient efficiency and rhizosphere microbiome,especially when maize is intercropped with different soybean varieties.In this study,field experiments were conducted to compare the growth and yield of two soybean(Glycine max)varieties,BD2 and YC03-3,and one maize(Zea mays)variety,Huazhen,in mono-cropped and intercropped cultures.The plant biomass and N content of both crops in BD2/maize intercropping were significantly improved compared to their monoculture,but no such effects were observed in the plants of YC03-3/maize intercropping.The yield of BD2 intercropped with maize exhibited a 37.5%increment above that of BD2 in monoculture.Moreover,19.2-29.1%longer root length of maize and 19.0-39.4%larger root volume of BD2 were observed in BD2/maize intercropping than in monoculture,but no growth advantage was observed in YC03-3/maize intercropping.Maize showed root avoidance when intercropped with BD2,but space competition when intercropped with YC03-3.16S rRNA amplicon sequencing showed that compared with the monoculture system,rhizobacteria community composition in BD2/maize intercropping changed more significantly than that of the YC03-3/maize intercropping system.In BD2/maize intercropping,most of the rhizobacteria community biomarker bacteria of BD2 were positively correlated with plant biomass,as well as plant P and N content.Maize tended to recruit Rhizobiales and Proteobacteria,which showed positive correlation with plant biomass and N content,respectively,as well as soil available N.In conclusion,soybean varieties determined the advantages of maize/soybean intercropping through root-root interactions and modification of rhizobacteria communities.Our insight emphasizes a linkage between root traits and the rhizobacteria community,which shows the importance of optimizing intercropping systems by selection of appropriate crop varieties.展开更多
Soybean mosaic virus(SMV)poses a substantial threat to the yield and quality of soybean(Glycine max(L.)Merr.),leading to significant economic losses in soybean production.However,the mining of SMVresistance loci and t...Soybean mosaic virus(SMV)poses a substantial threat to the yield and quality of soybean(Glycine max(L.)Merr.),leading to significant economic losses in soybean production.However,the mining of SMVresistance loci and the exploration of the underlying disease resistance mechanisms remain relatively limited.MicroRNAs(miRNAs)are a class of post-transcriptional regulators that play a pivotal role in modulating plant growth,development and responding to various stresses.In this study,we demonstrated the function of the “miR398c/d-GmCSDs”module between soybean resistant and susceptible varieties,focusing on its differential regulatory roles in SMV infection.Specifically,SMV infection downregulated gma-miR398c/d expression in the resistant variety(Qihuang 1,QH),while upregulated them in the susceptible variety(Nannong 1138-2,NN).Transient expression assay in N.benthamiana confirmed that gma-miR398c/d can target six superoxide dismutase(SOD)family genes,which responded to SMV infection in both varieties.Stable overexpression of Gma-MIR398c/d in soybean or inhibition of the corresponding target genes’expression via Bean pod mottle virus(BPMV)-induced gene silencing(VIGS)led to reduced H_(2)O_(2)content and thereby promoted SMV infection.Conversely,plants overexpressing the target genes exhibited the opposite phenotypes.The functions of gma-miR398c/d and their target genes were further validated in N.benthamiana through transient co-expression with SMV infectious clone(pSC7-GFP),indicating that gma-miR398c/d negatively regulated soybean resistance to SMV,while the target genes positively contributed to disease resistance.Collectively,our findings provide novel insights into the regulatory mechanisms underlying soybean resistance to SMV.展开更多
As one of the world's three major food crops and an important economic and oil crop,soybean plays a crucial role in ensuring food safety.In recent years,there are many problems in soybean cultivation,production an...As one of the world's three major food crops and an important economic and oil crop,soybean plays a crucial role in ensuring food safety.In recent years,there are many problems in soybean cultivation,production and processing.In view of this situation,this paper comprehensively expounded and decomposed the cultivation situation,existing problems,specific countermeasures and conclusions,so as to re-recognize them.This study provides reference materials for the sustainable and healthy development of the soybean industry.展开更多
Leaf-color mutants have proven valuable for studying chlorophyll metabolism,photosynthesis,and yield improvement.In this study,we identified a yellow-leaf(yl)mutant in soybean(Glycine max),characterized by reduced chl...Leaf-color mutants have proven valuable for studying chlorophyll metabolism,photosynthesis,and yield improvement.In this study,we identified a yellow-leaf(yl)mutant in soybean(Glycine max),characterized by reduced chlorophyll accumulation,lower net photosynthesis rate,and fewer grain number per plant than the wild type.To identify genes associated with chlorophyll content,we performed a largescale linkage mapping study using recombinant inbred lines from a cross between the yl mutant and a green-leaf cultivar across three environments.Using quantitative trait locus sequencing(QTL-seq)analysis,we mapped 12 QTL to chromosomes 5,13,15,19,and 20.Of these QTL,one new dominant locus with the largest LOD,named qCC1,was identified consistently and explained 31.73% of the total phenotypic variation on average.Notably,qCC1 was also associated with yield-related traits,including plant height and pod number per plant.Fine-mapping narrowed down qCC1 to an 82.29-kb region.Within this interval,we identified Glyma.15 g087500.1,encoding an ankyrin repeat-containing protein,as the most likely candidate gene,because its homologs are reported to function in thylakoid membrane biogenesis during plastid development.Phenotypic analysis of near-isogenic lines(NILs)revealed that those harboring the qCC1 allele conferring green leaves displayed significantly enhanced chlorophyll content by 136.53%-323.92%,net photosynthesis rate by 11.64%-42.13%,and yield by 111.32% compared with NILs carrying the allele conferring yellow leaves.Comparative transcriptome profiling of NILs coupled with RT-qPCR validation demonstrated that qCC1 up-regulated one differentially expressed gene(DEG)associated with chlorophyll biosynthesis and six DEGs related to photosystem,whereas it downregulated one gene involved in chlorophyll degradation.These findings provide valuable insights into the biological function and regulatory mechanism of chlorophyll metabolism and offer guidance for breeding soybean varieties with enhanced photosynthetic efficiency and high yield.展开更多
This study evaluated agro-morphological traits,grain quality traits,and phenotypic stability of nine soybean genotypes across four environments over two growing seasons(2022–2023).Traits such as days to flowering,day...This study evaluated agro-morphological traits,grain quality traits,and phenotypic stability of nine soybean genotypes across four environments over two growing seasons(2022–2023).Traits such as days to flowering,days to maturity,and disease symptoms were monitored throughout the season.At maturity,parameters including grain yield,grain oil and protein content,1000-seed weight,test weight,scores of lodgings,shattering,plant height,and first pod height were evaluated.In addition,18 morphological traits were scored,of which eight were sufficiently variable for further analysis.In terms of environmental stability,genotypes 2 and 13 excelled,while genotypes 12 and 14 consistently yielded the maximum grain production regardless of season or location.Through the application of PCA,we observed significant genotypic variance along with distinct clustering patterns based on year and location,with overall higher yields observed in the 2023 season.The 2022 season was marked by uneven rainfall that contributed to greater disease pressure.Grain yield exhibited a positive association with test weight and the timing of flowering/maturity,whereas lodging demonstrated a weak yet positive correlation.Although the lodging ratings were generally modest,there seems to be an indirect association between genotypes with somewhat higher scores and enhanced grain yield.Among morphological traits,the intensity of green leaf color and leaf blistering had the strongest influence on grain yield.This study shed information on key distinctions in characteristics and how they relate to grain yield,which could lead to future improvements in output.Some genotypes demonstrated both high yield potential and stability,which are essential for developing cultivars suitable for diverse environments.展开更多
Soybean(Glycine max)is a vital foundation of global food security,providing a primary source of highquality protein and oil for human consumption and animal feed.The rising global population has significantly increase...Soybean(Glycine max)is a vital foundation of global food security,providing a primary source of highquality protein and oil for human consumption and animal feed.The rising global population has significantly increased the demand for soybeans,emphasizing the urgency of developing high-yield,stresstolerant,and nutritionally superior cultivars.The extensive collection of soybean germplasm resources—including wild relatives,landraces,and cultivars—represents a valuable reservoir of genetic diversity critical for breeding advancements.Recent breakthroughs in genomic technologies,particularly highthroughput sequencing and multi-omics approaches,have revolutionized the identification of key genes associated with essential agronomic traits within these resources.These innovations enable precise and strategic utilization of genetic diversity,empowering breeders to integrate traits that improve yield potential,resilience to biotic and abiotic stresses,and nutritional quality.This review highlights the critical role of genetic resources and omics-driven innovations in soybean breeding.It also offers insights into strategies for accelerating the development of elite soybean cultivars to meet the growing demands of global soybean production.展开更多
Soybean is widely used in diets,and numerous reports have highlighted its antioxidant properties.However,constructing a methodology for rapid identifying and predicting a series of antioxidant active ingredients in So...Soybean is widely used in diets,and numerous reports have highlighted its antioxidant properties.However,constructing a methodology for rapid identifying and predicting a series of antioxidant active ingredients in Soybean presents certain challenges.Therefore,we introduced the spectrum-effect relationship-ingredient knockout identification technique to identify a series of antioxidant active ingredients in soybean.By combining untargeted metabolomics with network pharmacology,we predicted the antioxidant active ingredients and their target sites.We successfully identified 4 antioxidant active compounds(daidzein,genistein,daidzein,and glycitin)and 10 corresponding antioxidant targets(epidermal growth factor receptor(EGFR),estrogen receptor 1(ESR1),steroid receptor coactivator(SRC),tumor necrosis factor(TNF),kinase insert domain receptor(KDR),AKT serine/threonine kinase 1(AKT1),growth factor receptor bound protein 2(GRB2),signal transducer and activator of transcription1(STAT1),mitogen-activated protein kinase 8(MAPK8),B-cell lymphoma-2(BCL2))by our analysis.The validation results from cell experiments revealed that glycitin exhibited the best antioxidant activity and significantly influenced the expression of EGFR and the proteins associated with nuclear factor erythroid 2-related factor 2/NAD(P)H quinone dehydrogenase 1(NRF2/NQO1)signaling pathways.These findings were consistent with the predicted outcomes and were further confirmed in a zebrafish model.It suggests that glycitin may exert antioxidant effects by regulating the expression of EGFR,NRF2,and NQO1 proteins.The results demonstrate that a rapid analytical method for determining antioxidant activity was established.展开更多
[Objectives]To address the weeding challenges within the corn and soybean strip intercropping field and identify appropriate herbicide types and application methods suitable for the corn and soybean strip intercroppin...[Objectives]To address the weeding challenges within the corn and soybean strip intercropping field and identify appropriate herbicide types and application methods suitable for the corn and soybean strip intercropping fields in Siyang County.[Methods]The trial comprised six herbicide treatments and one blank control,with investigations conducted to assess efficacy,safety,and yield.[Results]Each herbicide treatment effectively controlled weeds,demonstrated high safety,and enhanced the yields of both soybeans and corn.The combined application of soil sealing with stem and leaf spray exhibited superior overall weed control compared to soil sealing alone.At 28 d following stem and leaf spray,the plant control effect and fresh weight control effect against weeds in the combined treatment of soil sealing with stem and leaf spray all exceeded 89%.[Conclusions]This study offers technical support for advancing the practice of strip intercropping between corn and soybeans.展开更多
基金supported by the National Natural Science Foundation of China(32302774)Beijing Innovation Consortium of Agriculture Research System(BAIC04)+1 种基金China Agriculture Research System(CARS-40)the Agricultural Science and Technology Innovation Program of the Feed Research Institute of the Chinese Academy of Agricultural Sciences(CAASIFR-ZDRW202402).
文摘Background Organic selenium(Se)has gained recognition in poultry nutrition as a feed additive to boost production and Se deposition in eggs and tissues,owing to its high bioavailability,efficient tissue accumulation and minimal toxicity.Selenium-enriched yeast(SeY)is a well-established source,while selenium-enriched lactobacilli(SeL),a newer alternative,offers the added benefits of probiotics.This study examined the effects of SeY and SeL on egg quality,antioxidant capacity,Se deposition,and gut health in laying hens.After a two-week pre-treatment with a Sedeficient diet(SeD),450 Hy-Line Brown laying hens(30-week-old)were assigned into five dietary groups with six replicates of 15 hens each.The groups included a SeD,SeD supplemented with 1.5 mg Se/kg from SeY(SeY15),or 1.5,3.0,and 6.0 mg Se/kg from SeL(SeL15,SeL30,SeL60).The feeding trial lasted for 12 weeks.Results SeY15 and SeL15 improved the feed-to-egg ratio(P<0.05)in the latter stages.Haugh units were significantly increased(P<0.05)in the SeY15 and SeL30 groups,while darker yolk color(P<0.05)was observed in the SeY15,SeL15,and SeL60 groups.All Se-supplemented diets increased Se content in whole eggs,albumen,and yolk(P<0.05),while SeL groups showed a dose-dependent effect.Antioxidant enzyme activities increased,and MDA content decreased in the serum(P<0.05),with SeY15 showing the highest GSH-Px levels(P<0.05).SeL60 increased serum alkaline phosphatase and aspartate transaminase,and distorted the liver architecture(P<0.05).Se-diets reduced concentrations of reactive oxygen species(ROS)in the ileum and liver(P<0.05).SeL15 improved the ileal villus height-tocrypt depth ratio(P<0.05).SeY15 and/or SeL15 up-regulated TXNRD1 and SEPHS1 mRNA while down-regulating SCLY expression in the liver.SeY15 altered ileal microbiota by increasing both beneficial and pathogenic bacteria,whereas SeL15 predominantly boosted beneficial bacteria.Conclusion SeL integrates the antioxidant properties of organic Se with the probiotic benefits on gut health,resulting in a performance-enhancing effect comparable to that of SeY.However,high SeL level(6.0 mg Se/kg)compromised productivity and metabolic functions while enhancing Se deposition.
基金National Natural Science Foundation of China(Nos.81872917 and 81741164)。
文摘The objective of this study was to verify the protective effect of Bifidobacterium longum(BL) and the synergistical effect of Selenium and BL on alcohol plus high fat diet(HFD) induced hepatic injury in mice. We also want to explore the mechanism of Selenium-enriched Bifidobacterium longum(Se BL). C57 BL/6 mice were treated with alcohol plus HFD with or without different dosage of BL or Se BL for 4 weeks. Serum levels of ALT, AST, TC, TG, LDL-C, HDL-C, FFAs, TNF-α, IL-6 and IL-1β, hepatic MDA level, SOD activity, the m RNA levels of AMPK, PPAR-α and SREBP1 were invested. Se BL inhibited lipid accumulation in hepatocytes;reduced serum AST and ALT levels;improved dyslipidemia;decreased serum FFAs, TC, TG and LDL-C levels. Se BL also inhibited alcohol plus HFD-induced hepatocyte oxidative stress through decrease in hepatic MDA levels and increase in SOD activity. Se BL also regulated lipid metabolism related genes such as AMPK, PPAR-α and SREBP1. Although BL had similar effect as Se BL, Se BL is more effective than BL. Se BL protected mice from alcohol plus HFD-induced hepatic injury in mice because of its inhibitory effect on hepatocellular oxidative stress, lipogenesis and inflammation. Selenium enhanced the protective effect of BL.
基金financial sponsored by the National Key R&D Program of China(No.2018YFC1604405)Fund of Shanghai Engineering Research Center of Plant Germplasm Resources(No.17DZ2252700)Research on the health function of tea and deep-processed products in preventing metabolic diseases(No.C-6105-20-074)。
文摘High-salt diet is well recognized as a risk factor for hypertension,and dietary intervention plays a critical role in the prevention of hypertension.The current study investigated the effects of selenium-enriched green tea(Se-GT)and ordinary green tea(GT)on prevention of hypertension of rats induced by high-salt diet,as well as their potential regulatory and mechanism.Our results showed that GT and Se-GT supplementations significantly prevented the increase of blood pressure(BP),activated the phosphoinosmde-3-kinase/protein kinase B(PI3K/Akt)signaling pathway,and regulated the gene expression related to BP,as well as improved the tissue damage like heart,liver,and kidneys.Besides,the key parameters associated with oxidative stress,inflammation and endothelial dysfunction were also altered by GT and Se-GT treatments.Importantly,GT or Se-GT administration adjusted the diversity and composition of the intestinal flora.Moreover,GT and Se-GT supplementations increased the abundance of beneficial bacteria and reduced the abundance of harmful or conditional pathogenic bacteria.More specifically,GT intake specifically and significantly enriched the relative abundance of Paraprevotella and Bacteroides,whereas Se-GT was characterized by specific and significant enrichment for Allobaculum and Bifidobacterium.Our results proved that dietary supplement of GT and Se-GT remarkably improved the vascular functions and effectively prevented tissue damage by regulation of intestinal flora,and thus preventing hypertension induced by high-salt diet.
基金National Key R&D Program of China(2018YFD1100202)Shaanxi Social Science Fund Program of China(2018S30)Shaanxi Undergraduate Innovation Planning Project of China(201829041).
文摘As an important symbol of agricultural and sideline products in Ankang City,selenium-enriched tea affects the development of regional agricultural and sideline industries.In this paper,the development status and problems of the selenium-enriched tea products of Ankang were analyzed in terms of capital,technology,management and sales mode.This research is of positive significance to the development of selenium-enriched products and other agricultural and sideline products of health value,and plays a very important role in promoting the cultivation of village-level characteristic industries and the effective realization of targeted poverty alleviation.
基金Supported by the Science and Technology Major Project of Guangxi(Guike AA17202026)Program for Scientific Research and Technology Development in Xixiangtant District of Nanning City(201710304)+7 种基金the Special Fund for the Innovation-Driven Development in Guangxi(Guike AA17202019-4&AA17202019)the Science and Technology Development Fund of Guangxi Academy of Agricultural Sciences(Guinongke 2017JM03)the Program for the Scientific Research and Technology Development in Guangxi(Guikehe415104001-22)the Fundamental Research Funds for Guangxi Academy of Agricultural Sciences(Guinongke 2017YZ03)the Key Research and Development Program of Guangxi(Guike AB16380088)the Experiment Station for Selenium Featured Crops in Guangxi(Gui TS2016011)the Key Research and Development Program of Qingxiu District,Guangxi(2016039)the Scientific and Technological Transformative Project of Guangxi Academy of Agricultural Sciences(NO.2017NZ04)
文摘[Objectives] The aims were to optimize the extraction process of selenoproteins from selenium-enriched rice in Guangxi and provide references for the intensive processing and comprehensive utilization of selenium protein resources. [Methods]Selenium-enriched rice was used as materials to extract selenoproteins by phosphate buffer extraction method and to optimize the extraction process of selenoproteins by using the orthogonal experiment. Proteins and selenium content was measured by Coomassie Brilliant Blue G-250 reagent and AFS（ atomic fluorescence spectrometry） respectively. [Results] The most significant factor affecting extraction of rice Selenoproteins was extraction NaO H concentration,followed by the ratio of solid-liquid,temperature and then extraction time. The optimum extraction conditions of selenoproteins from rice were extraction temperature of 50 ℃,NaO H concentration of 0. 14 mol/L,extraction time of 5 h,and solid-liquid ratio of 1∶ 30. [Conclusions]The alkali extraction process optimized by orthogonal test could effectively improve the extraction rate of selenoproteins,and the optimized process parameters could be popularized and applied in practical production.
文摘Selenium is an essential trace element for human health.Eating selenium-enriched eggs is conducive to easily and effectively solving the problem of selenium deficiency.In the production process of selenium-enriched eggs,different selenium sources,additive doses,feeding time,and the addition of other vitamins,trace elements,and methionine in the diet will influence the deposition of selenium in eggs.Through this review,it is intended to provide a reference for the practical,safe and economical production of selenium-enriched eggs.
基金Supported by Key Science and Technology Project for Innovation-driven Development of Guangxi"High Efficient and Safe Use of Selenium-enriched Soil Resources"(2017AA19015)Scientific Research and Technological Development Program Project of Guangxi Province(Gui Ke He 415104001-22)+2 种基金Key Research and Development Program of Guangxi(Gui Ke AB16380088 and Gui Ke AB16380164)Scientific Research and Technological Development Program Project of Nanning City(20152054-13)Scientific Research and Technological Development Program of Xixiangtang District of Nanning City(2015312)
文摘Taking Scientific Outlook on Development as the guiding principle,this paper introduced the principles of innovation,coordination,green,openness and sharing. Based on gifted advantages of selenium-enriched soil and selenium-enriched industry development in Guangxi,it discussed current practice,stressed green,ecological,characteristic demonstration,transformation and upgrading,and targeted poverty alleviation. Finally,it came up with perspective,practical,and operational strategies and recommendations for development of selenium-enriched industry,to provide reference for sustainable development of selenium-enriched functional industry in Guangxi.
文摘In order to improve the added value of the main vegetable products in Qinghai Province,we researched the effect of soil selenium content on vegetable quality in selenium-enriched area of Qinghai. Through the analysis of Qinghai selenium-enriched soil selenium content and5 kinds of main vegetables corresponds to the total selenium,part of the mineral elements and soluble sugar and VC content,the effects of soil selenium levels on selenium absorption and nourishment quality of vegetables were researched. The results showed that he Qinghai selenium-enriched soil selenium content in the range of 100. 00-563. 00 μg/kg,among them,the soil samples with sufficient selenium and rich selenium accounted for 76. 53% and 18. 88%,respectively. Soil selenium content of garlic sampling area was relatively high,and soil selenium content of sugar beet sampling area was relatively low. Vegetable selenium content was in the range of 11. 00-340. 94 μg/kg,the average content of total selenium content of garlic was up to 170. 40 μg/kg,and the average content of the total selenium content of radish is 73. 00 μg/kg.90. 63% of the vegetables in the region reached the level of sufficient selenium,and 70. 31% reached the level of selenium enrichment. The average content of Ca and Mg was higher than the national average. There was no significant correlation between selenium content of soil and vegetable and nutritional quality.
文摘To investigate the in vitro digestion and fermentation properties of soybean oligosaccharides(SBOS)extracted from defatted soybean meal,the changes in monosaccharide composition and molecular mass were analyzed.Subsequently,the effect of SBOS on microbial community structure and metabolites was studied by 16S rRNA gene sequencing and untargeted metabolomics based on liquid chromatography-mass spectrometry.Results showed that SBOS was not easily enzymolyzed during simulated digestion and could reach the large intestine through the digestive system.The significant decrease in the molecular mass of SBOS after in vitro fermentation indicated its utilization by the gut microbiota,which increased the contents of short-chain fatty acids and lactic acid,thereby reducing the pH of the fermentation broth.Moreover,the core community was found to consist of Blautia,Lactobacillaceae,and Pediococcus.SBOS up-regulated beneficial differential metabolites such as myo-inositol,lactose,and glucose,which were closely related to galactose,amino sugar,and nucleotide sugar metabolism.This study will provide a reference for exploring the relationship between the gut microbiota and the metabolites of SBOS,and provide a basis for the development and application of SBOS as an ingredient for functional products.
基金supported by the Biological Breeding-National Science and Technology Major Project(2023ZD0403305)National Natural Science Foundation of China(32101845)+1 种基金the National Key Research and Development Program of China(2023YFE0105000)the China Agriculture Research System(CARS-04).
文摘Dense cropping increases crop yield but intensifies resource competition,which reduces single plant yield and limits potential yield growth.Optimizing canopy spacing could enhance resource utilization,support crop morphological development and increase yield.Here,a three-year study was performed to verify the feasibility of adjusting row spacing to further enhance yield in densely planted soybeans.Of three row-spacing configurations(40-40,20-40,and 20-60 cm)and two planting densities(normal 180,000 plants ha 1 and high 270,000 plants ha 1).The differences in canopy structure,plant morphological development,photosynthetic capacity and their impact on yield were analyzed.Row spacing configurations have a significant effect on canopy transmittance(CT).The 20-60 cm row spacing configuration increased CT and creates a favorable canopy light environment,in which plant height is reduced,while branching is promoted.This approach reduces plant competition,optimizes the developments of leaf area per plant,specific leaf area,leaf area development rate,leaf area duration and photosynthetic physiological indices(F_(v)/F_(m),ETR,P_(n)).The significant increase of 11.9%-34.2%in canopy apparent photosynthesis(CAP)is attributed to the significant optimization of plant growth and photosynthetic physiology through CT,an important contributing factor to yield increases.The yield in the 20-60 cm treatment is 4.0%higher than in equidistant planting under normal planting density,but 5.9%under high density,primarily driven by CAP and pod number.These findings suggest that suitable row spacing configurations optimize the light environment for plants,promote source-sink transformation in soybeans,and further improve yield.In practice,a 20-60 cm row spacing configuration could be employed for high-density soybean planting to achieve a more substantial yield gain.
基金supported by National Natural Science Foundation of China(32470265)to GDW.
文摘Plant specialized metabolites are commonly stored in glycosylated forms within plant cells,with their homeostasis regulated by glycosyltransferases andβ-glucosidases(BGLUs,also known asβ-glucoside hydrolases(E.C.3.2.1.21)).Soyasaponins,the predominant triterpenoid compounds(C30)in soybean seeds,contain two sugar moieties attached at the C3 and C22 positions.While glycosyltransferases involved in soyasaponin biosynthesis have been well characterized,the role of BGLUs in soyasaponin homeostasis remains unclear.In this study,we identified GmSSBG1(Soyasaponinβ-glucosidase1;Glyma.07G258700)as a candidate gene potentially involved in soyasaponin homeostasis through gene to gene co-expression analysis.Biochemical assays demonstrated that GmSSBG1 specifically hydrolyzes arabinose residues at the C22 position of A0-and B0-series soyasaponins.Loss-of-function mutations in GmSSBG1 led to a significant accumulation of A0-and B0-series soyasaponins in mutant seeds,which correlated with a pronounced decrease in resistance to the soybean pod borer(Leguminivora glycinivorella).Our findings provide critical insights into the regulatory mechanisms underlying soyasaponin homeostasis and lay a theoretical foundation for molecular breeding strategies aimed at developing soybean lines with enhanced resistance to soybean pod borer,even to other insect pests.
基金supported by the Ten-thousand Talents Plan of Zhejiang Province(No.2022R52021)the Key Research and Development Program of Zhejiang Province(No.2021C04016)the Pioneer and Leading Goose R&D Program of Zhejiang(No.2022C04020),China.
文摘Soybean meal(SBM)prepared by soybean crushing is the most popular protein source in the poultry and livestock industries(Cai et al.,2015)due to its economic manufacture,high protein content,and good nutritional value.Despite these benefits,SBM contains various antigen proteins such as glycinin andβ-conglycinin,which account for approximately 70%of the total proteins of the SBM and reduce digestibility and damage intestinal function(Peng et al.,2018).
基金supported by the National Key Research and Development Program of China(2021YFF1000504 and 2023YFD1901300)the National Natural Science Foundation of China(32172658,32172659 and 32302662)the Natural Science Foundation of Guangdong Province,China(2021A1515010826).
文摘Maize/soybean intercropping systems are commonly used in developing countries,but few studies have been performed to elucidate the differences in nutrient efficiency and rhizosphere microbiome,especially when maize is intercropped with different soybean varieties.In this study,field experiments were conducted to compare the growth and yield of two soybean(Glycine max)varieties,BD2 and YC03-3,and one maize(Zea mays)variety,Huazhen,in mono-cropped and intercropped cultures.The plant biomass and N content of both crops in BD2/maize intercropping were significantly improved compared to their monoculture,but no such effects were observed in the plants of YC03-3/maize intercropping.The yield of BD2 intercropped with maize exhibited a 37.5%increment above that of BD2 in monoculture.Moreover,19.2-29.1%longer root length of maize and 19.0-39.4%larger root volume of BD2 were observed in BD2/maize intercropping than in monoculture,but no growth advantage was observed in YC03-3/maize intercropping.Maize showed root avoidance when intercropped with BD2,but space competition when intercropped with YC03-3.16S rRNA amplicon sequencing showed that compared with the monoculture system,rhizobacteria community composition in BD2/maize intercropping changed more significantly than that of the YC03-3/maize intercropping system.In BD2/maize intercropping,most of the rhizobacteria community biomarker bacteria of BD2 were positively correlated with plant biomass,as well as plant P and N content.Maize tended to recruit Rhizobiales and Proteobacteria,which showed positive correlation with plant biomass and N content,respectively,as well as soil available N.In conclusion,soybean varieties determined the advantages of maize/soybean intercropping through root-root interactions and modification of rhizobacteria communities.Our insight emphasizes a linkage between root traits and the rhizobacteria community,which shows the importance of optimizing intercropping systems by selection of appropriate crop varieties.
基金supported by the National Key Research and Development Program of China(2022YFF1001502)Jiangsu Collaborative Innovation Center for Modern Crop Production(JCIC-MCP)Major Projects of Technological Innovation 2030(2023ZD04037).
文摘Soybean mosaic virus(SMV)poses a substantial threat to the yield and quality of soybean(Glycine max(L.)Merr.),leading to significant economic losses in soybean production.However,the mining of SMVresistance loci and the exploration of the underlying disease resistance mechanisms remain relatively limited.MicroRNAs(miRNAs)are a class of post-transcriptional regulators that play a pivotal role in modulating plant growth,development and responding to various stresses.In this study,we demonstrated the function of the “miR398c/d-GmCSDs”module between soybean resistant and susceptible varieties,focusing on its differential regulatory roles in SMV infection.Specifically,SMV infection downregulated gma-miR398c/d expression in the resistant variety(Qihuang 1,QH),while upregulated them in the susceptible variety(Nannong 1138-2,NN).Transient expression assay in N.benthamiana confirmed that gma-miR398c/d can target six superoxide dismutase(SOD)family genes,which responded to SMV infection in both varieties.Stable overexpression of Gma-MIR398c/d in soybean or inhibition of the corresponding target genes’expression via Bean pod mottle virus(BPMV)-induced gene silencing(VIGS)led to reduced H_(2)O_(2)content and thereby promoted SMV infection.Conversely,plants overexpressing the target genes exhibited the opposite phenotypes.The functions of gma-miR398c/d and their target genes were further validated in N.benthamiana through transient co-expression with SMV infectious clone(pSC7-GFP),indicating that gma-miR398c/d negatively regulated soybean resistance to SMV,while the target genes positively contributed to disease resistance.Collectively,our findings provide novel insights into the regulatory mechanisms underlying soybean resistance to SMV.
基金Supported by Special Fund for National Modern Agricultural Industry Technology System Construction(CARS-04-CES16).
文摘As one of the world's three major food crops and an important economic and oil crop,soybean plays a crucial role in ensuring food safety.In recent years,there are many problems in soybean cultivation,production and processing.In view of this situation,this paper comprehensively expounded and decomposed the cultivation situation,existing problems,specific countermeasures and conclusions,so as to re-recognize them.This study provides reference materials for the sustainable and healthy development of the soybean industry.
基金supported by the Heilongjiang Provincial Natural Science Foundation of China(SS2023C002)。
文摘Leaf-color mutants have proven valuable for studying chlorophyll metabolism,photosynthesis,and yield improvement.In this study,we identified a yellow-leaf(yl)mutant in soybean(Glycine max),characterized by reduced chlorophyll accumulation,lower net photosynthesis rate,and fewer grain number per plant than the wild type.To identify genes associated with chlorophyll content,we performed a largescale linkage mapping study using recombinant inbred lines from a cross between the yl mutant and a green-leaf cultivar across three environments.Using quantitative trait locus sequencing(QTL-seq)analysis,we mapped 12 QTL to chromosomes 5,13,15,19,and 20.Of these QTL,one new dominant locus with the largest LOD,named qCC1,was identified consistently and explained 31.73% of the total phenotypic variation on average.Notably,qCC1 was also associated with yield-related traits,including plant height and pod number per plant.Fine-mapping narrowed down qCC1 to an 82.29-kb region.Within this interval,we identified Glyma.15 g087500.1,encoding an ankyrin repeat-containing protein,as the most likely candidate gene,because its homologs are reported to function in thylakoid membrane biogenesis during plastid development.Phenotypic analysis of near-isogenic lines(NILs)revealed that those harboring the qCC1 allele conferring green leaves displayed significantly enhanced chlorophyll content by 136.53%-323.92%,net photosynthesis rate by 11.64%-42.13%,and yield by 111.32% compared with NILs carrying the allele conferring yellow leaves.Comparative transcriptome profiling of NILs coupled with RT-qPCR validation demonstrated that qCC1 up-regulated one differentially expressed gene(DEG)associated with chlorophyll biosynthesis and six DEGs related to photosystem,whereas it downregulated one gene involved in chlorophyll degradation.These findings provide valuable insights into the biological function and regulatory mechanism of chlorophyll metabolism and offer guidance for breeding soybean varieties with enhanced photosynthetic efficiency and high yield.
文摘This study evaluated agro-morphological traits,grain quality traits,and phenotypic stability of nine soybean genotypes across four environments over two growing seasons(2022–2023).Traits such as days to flowering,days to maturity,and disease symptoms were monitored throughout the season.At maturity,parameters including grain yield,grain oil and protein content,1000-seed weight,test weight,scores of lodgings,shattering,plant height,and first pod height were evaluated.In addition,18 morphological traits were scored,of which eight were sufficiently variable for further analysis.In terms of environmental stability,genotypes 2 and 13 excelled,while genotypes 12 and 14 consistently yielded the maximum grain production regardless of season or location.Through the application of PCA,we observed significant genotypic variance along with distinct clustering patterns based on year and location,with overall higher yields observed in the 2023 season.The 2022 season was marked by uneven rainfall that contributed to greater disease pressure.Grain yield exhibited a positive association with test weight and the timing of flowering/maturity,whereas lodging demonstrated a weak yet positive correlation.Although the lodging ratings were generally modest,there seems to be an indirect association between genotypes with somewhat higher scores and enhanced grain yield.Among morphological traits,the intensity of green leaf color and leaf blistering had the strongest influence on grain yield.This study shed information on key distinctions in characteristics and how they relate to grain yield,which could lead to future improvements in output.Some genotypes demonstrated both high yield potential and stability,which are essential for developing cultivars suitable for diverse environments.
基金supported by the National Key Research and Development Program of China(2022YFF1003301,2023YFF1000101,2022YFE0130200)the Taishan Scholars Program。
文摘Soybean(Glycine max)is a vital foundation of global food security,providing a primary source of highquality protein and oil for human consumption and animal feed.The rising global population has significantly increased the demand for soybeans,emphasizing the urgency of developing high-yield,stresstolerant,and nutritionally superior cultivars.The extensive collection of soybean germplasm resources—including wild relatives,landraces,and cultivars—represents a valuable reservoir of genetic diversity critical for breeding advancements.Recent breakthroughs in genomic technologies,particularly highthroughput sequencing and multi-omics approaches,have revolutionized the identification of key genes associated with essential agronomic traits within these resources.These innovations enable precise and strategic utilization of genetic diversity,empowering breeders to integrate traits that improve yield potential,resilience to biotic and abiotic stresses,and nutritional quality.This review highlights the critical role of genetic resources and omics-driven innovations in soybean breeding.It also offers insights into strategies for accelerating the development of elite soybean cultivars to meet the growing demands of global soybean production.
基金supported by National Key R&D Program of China(2022YFF1100300)Joint Fund of Henan Province Science and Technology R&D Program(235200810051)+1 种基金Key Project in Science and Technology Agency of Henan Province(242102310561)key research projects of higher education institutions in Henan Province(24B350002).
文摘Soybean is widely used in diets,and numerous reports have highlighted its antioxidant properties.However,constructing a methodology for rapid identifying and predicting a series of antioxidant active ingredients in Soybean presents certain challenges.Therefore,we introduced the spectrum-effect relationship-ingredient knockout identification technique to identify a series of antioxidant active ingredients in soybean.By combining untargeted metabolomics with network pharmacology,we predicted the antioxidant active ingredients and their target sites.We successfully identified 4 antioxidant active compounds(daidzein,genistein,daidzein,and glycitin)and 10 corresponding antioxidant targets(epidermal growth factor receptor(EGFR),estrogen receptor 1(ESR1),steroid receptor coactivator(SRC),tumor necrosis factor(TNF),kinase insert domain receptor(KDR),AKT serine/threonine kinase 1(AKT1),growth factor receptor bound protein 2(GRB2),signal transducer and activator of transcription1(STAT1),mitogen-activated protein kinase 8(MAPK8),B-cell lymphoma-2(BCL2))by our analysis.The validation results from cell experiments revealed that glycitin exhibited the best antioxidant activity and significantly influenced the expression of EGFR and the proteins associated with nuclear factor erythroid 2-related factor 2/NAD(P)H quinone dehydrogenase 1(NRF2/NQO1)signaling pathways.These findings were consistent with the predicted outcomes and were further confirmed in a zebrafish model.It suggests that glycitin may exert antioxidant effects by regulating the expression of EGFR,NRF2,and NQO1 proteins.The results demonstrate that a rapid analytical method for determining antioxidant activity was established.
文摘[Objectives]To address the weeding challenges within the corn and soybean strip intercropping field and identify appropriate herbicide types and application methods suitable for the corn and soybean strip intercropping fields in Siyang County.[Methods]The trial comprised six herbicide treatments and one blank control,with investigations conducted to assess efficacy,safety,and yield.[Results]Each herbicide treatment effectively controlled weeds,demonstrated high safety,and enhanced the yields of both soybeans and corn.The combined application of soil sealing with stem and leaf spray exhibited superior overall weed control compared to soil sealing alone.At 28 d following stem and leaf spray,the plant control effect and fresh weight control effect against weeds in the combined treatment of soil sealing with stem and leaf spray all exceeded 89%.[Conclusions]This study offers technical support for advancing the practice of strip intercropping between corn and soybeans.
