Selenium(Se)in paddy rice is one of the significant sources of human Se nutrition.However,the effect of arsenic(As)pollution in soil on the translocation of Se species in rice plants is unclear.In this research,a pot ...Selenium(Se)in paddy rice is one of the significant sources of human Se nutrition.However,the effect of arsenic(As)pollution in soil on the translocation of Se species in rice plants is unclear.In this research,a pot experiment was designed to examine the effect of the addition of 50 mg As/kg soil as arsenite or arsenate on the migration of Se species from soil to indica Minghui 63 and Luyoumingzhan.The results showed that the antagonism between inorganic As and Se was closely related to the rice cultivar and Se oxidation state in soil.Relative to the standalone selenate treatment,arsenite significantly(p<0.05)decreased the accumulation of selenocystine,selenomethionine and selenate in the roots,stems,sheaths,leaves,brans and kernels of both cultivars by 21.4%-100.0%,40.0%-100.0%,41.0%-100%,5.4%-96.3%,11.3%-100.0% and 26.2%-39.7% respectively,except for selenocystine in the kernels of indica Minghui 63 and selenomethionine in the leaves of indica Minghui 63 and the stems of indica Luyoumingzhan.Arsenate also decreased(p<0.05)the accumulation of selenocystine,selenomethionine and selenate in the roots,stems,brans and kernels of both cultivars by 34.9%-100.0%,30.2%-100.0%,11.3%-100.0% and 5.6%-39.6%respectively,except for selenate in the stems of indica Minghui 63.However,relative to the standalone selenite treatment,arsenite and arsenate decreased(p<0.05)the accumulation of selenocystine,selenomethionine and selenite only in the roots of indica Minghui 63 by 45.5%-100.0%.Our results suggested that arsenite and arsenate had better antagonism toward Se species in selenate-added soil than that in selenite-added soil;moreover,arsenite had a higher inhibiting effect on the accumulation of Se species than arsenate.展开更多
Selenium,as an essential trace element,exerts health effects that are contingent not only on the quantity consumed but also on the specific selenium species present in the dietary.The aim of this study was to investig...Selenium,as an essential trace element,exerts health effects that are contingent not only on the quantity consumed but also on the specific selenium species present in the dietary.The aim of this study was to investigate the effects of different selenium supplements on the distribution of selenium content,the forms of selenium,and its in vitro bioaccessibility in piglets.Inductively coupled plasma mass spectrometry and high-performance liquid chromatography(HPLC-ICP/MS)were applied to analyze the selenium content and its speciation in tissues and organs of piglets fed with three selenium supplements,sodium selenite(SS),selenium-enriched Cardamine hirsute(CH),and selenium-enriched yeast(SY).The bioaccessibility experiments on the livers of selenium-enriched piglets were conducted by simulating human gastrointestinal digestion.It was found that the kidney has the highest selenium content among the 12 examined organs.The efficacy of different selenium sources in improving the selenium content in piglets ranked in descending order,is as follows:SY,SS,and CH.The predominant form of selenium in the longissimus dorsi muscle is selenomethionine(SeMet),accompanied by smaller quantities of selenocysteine(SeCys)and methyl selenocysteine(MeSeCys).In contrast,the predominant selenium form in the liver is SeCys,along with SeMet and MeSeCys.Piglets fed with Se-enriched yeast exhibited a significant increase in the content and proportion of SeMet in both the longissimus dorsi muscle and liver.Additionally,different selenium species demonstrated varied G and GI bioaccessilities,with SeMet being the highest and SeCys the lowest.展开更多
基金supported by the National Natural Science Foundation of China(No.21677033)the Natural Science Foundation of Science and Technology Department of Fujian Government(No.2021N0005)Fujian Agriculture and Forestry University Innovation Fund(Nos.CXZX2020049A,KFb22047XA)。
文摘Selenium(Se)in paddy rice is one of the significant sources of human Se nutrition.However,the effect of arsenic(As)pollution in soil on the translocation of Se species in rice plants is unclear.In this research,a pot experiment was designed to examine the effect of the addition of 50 mg As/kg soil as arsenite or arsenate on the migration of Se species from soil to indica Minghui 63 and Luyoumingzhan.The results showed that the antagonism between inorganic As and Se was closely related to the rice cultivar and Se oxidation state in soil.Relative to the standalone selenate treatment,arsenite significantly(p<0.05)decreased the accumulation of selenocystine,selenomethionine and selenate in the roots,stems,sheaths,leaves,brans and kernels of both cultivars by 21.4%-100.0%,40.0%-100.0%,41.0%-100%,5.4%-96.3%,11.3%-100.0% and 26.2%-39.7% respectively,except for selenocystine in the kernels of indica Minghui 63 and selenomethionine in the leaves of indica Minghui 63 and the stems of indica Luyoumingzhan.Arsenate also decreased(p<0.05)the accumulation of selenocystine,selenomethionine and selenate in the roots,stems,brans and kernels of both cultivars by 34.9%-100.0%,30.2%-100.0%,11.3%-100.0% and 5.6%-39.6%respectively,except for selenate in the stems of indica Minghui 63.However,relative to the standalone selenite treatment,arsenite and arsenate decreased(p<0.05)the accumulation of selenocystine,selenomethionine and selenite only in the roots of indica Minghui 63 by 45.5%-100.0%.Our results suggested that arsenite and arsenate had better antagonism toward Se species in selenate-added soil than that in selenite-added soil;moreover,arsenite had a higher inhibiting effect on the accumulation of Se species than arsenate.
文摘Selenium,as an essential trace element,exerts health effects that are contingent not only on the quantity consumed but also on the specific selenium species present in the dietary.The aim of this study was to investigate the effects of different selenium supplements on the distribution of selenium content,the forms of selenium,and its in vitro bioaccessibility in piglets.Inductively coupled plasma mass spectrometry and high-performance liquid chromatography(HPLC-ICP/MS)were applied to analyze the selenium content and its speciation in tissues and organs of piglets fed with three selenium supplements,sodium selenite(SS),selenium-enriched Cardamine hirsute(CH),and selenium-enriched yeast(SY).The bioaccessibility experiments on the livers of selenium-enriched piglets were conducted by simulating human gastrointestinal digestion.It was found that the kidney has the highest selenium content among the 12 examined organs.The efficacy of different selenium sources in improving the selenium content in piglets ranked in descending order,is as follows:SY,SS,and CH.The predominant form of selenium in the longissimus dorsi muscle is selenomethionine(SeMet),accompanied by smaller quantities of selenocysteine(SeCys)and methyl selenocysteine(MeSeCys).In contrast,the predominant selenium form in the liver is SeCys,along with SeMet and MeSeCys.Piglets fed with Se-enriched yeast exhibited a significant increase in the content and proportion of SeMet in both the longissimus dorsi muscle and liver.Additionally,different selenium species demonstrated varied G and GI bioaccessilities,with SeMet being the highest and SeCys the lowest.
