In order to improve the performance of the extracellular selenium polysaccharide(SeNPs)of Weissella confusa XG-3 and its application in food industry,the effects of the addition ratio of exopolysaccharide(EPS)and sodi...In order to improve the performance of the extracellular selenium polysaccharide(SeNPs)of Weissella confusa XG-3 and its application in food industry,the effects of the addition ratio of exopolysaccharide(EPS)and sodium selenite,the reaction temperature,time and pH on the synthesis were optimized and the rheological property and cytotoxcity assay of SeNPs was studied.In this study,the optimal reaction conditions were determined as follows:the ratio of EPS to sodium selenite was 1∶1,reaction at 37℃for 6 h,pH 4.The characteristic peak of SeNPs appeared at 250~300 nm in full wavelength scanning,indicating the successful compounding of sodium selenite on EPS.SeNPs had higher apparent viscosity than EPS,which led to a more desirable compatibility during industrial application.Cytotoxicity experiments showed that SeNPs did not inhibit the growth of mouse macrophage RAW 264.7 at the concentration of 0~3 mg·mL^(-1),indicating that SeNPs was not toxic within this concentration range.展开更多
Type 2 diabetes(T2D)is a complex metabolic disease characterized by insulin resistance and chronic inflammation.This study explored the effects of sweet corn cob selenium polysaccharide(SeSCP),a complex of selenium an...Type 2 diabetes(T2D)is a complex metabolic disease characterized by insulin resistance and chronic inflammation.This study explored the effects of sweet corn cob selenium polysaccharide(SeSCP),a complex of selenium and sweet corn cob polysaccharide,on T2D.SeSCP demonstrated significant hypoglycemic effects.This investigation focused on the impact of SeSCP on the intestinal barrier,inflammation,and microbiota in streptozotocin(STZ)-induced T2D mice.These findings revealed that SeSCP effectively reduced blood glucose levels and insulin resistance,decreased intestinal barrier permeability,and modulated the LPS/IκBα/NFκB pathway to reduce inflammatory factor production.Additionally,SeSCP corrected intestinal flora imbalances,increasing the relative abundance of Firmicutes in T2D mice.Furthermore,SeSCP enhanced carbohydrate and lipid metabolism in T2D mice,as indicated by Kyoto Encyclopedia of Genes and Genomes(KEGG)analysis,demonstrating its potential to alleviate T2D through intestinal modulation.展开更多
Selenium-enriched polysaccharides refer to polysaccharides that contain selenium,which have emerged as a significant area of research in recent years due to the bioactivities associated with both polysaccharides and s...Selenium-enriched polysaccharides refer to polysaccharides that contain selenium,which have emerged as a significant area of research in recent years due to the bioactivities associated with both polysaccharides and selenium.In this study,we prepared Spirulina platensis polysaccharides(SPP)and selenium-enriched SPP(SeSPP),and assessed their structural properties and hypoglycaemic functions both in vitro and in vivo.The re-sults of the structural characterization indicated that SPP was successfully selenized.The interaction between SPP and selenium primarily occurred in the forms of Se-O-C and O-Se-O through covalent interactions.The molecular weight of SeSPP was found to be more concentrated compared to SPP,and its microscopic morphology exhibited greater complexity,including both rod-shaped and spherical particles.It was determined that SPP and SeSPP were mainly composed of glucose,rhamnose,and fucose.In vitro assays demonstrated that SeSPP exhibited significantly enhanced hypoglycaemic and antioxidant properties compared to SPP.In an in vivo model of streptozotocin-and high-fat diet-induced type 2 diabetes mice,SeSPP improved hypoglycaemic activity by reversing body weight,blood glucose levels,glucose tolerance,insulin resistance,lipid levels,hepatic oxidative stress levels,as well as liver and pancreatic morphology and function.Consequently,SeSPP may serve as a se-lenium supplement that could enhance hypoglycaemic effects.展开更多
文摘In order to improve the performance of the extracellular selenium polysaccharide(SeNPs)of Weissella confusa XG-3 and its application in food industry,the effects of the addition ratio of exopolysaccharide(EPS)and sodium selenite,the reaction temperature,time and pH on the synthesis were optimized and the rheological property and cytotoxcity assay of SeNPs was studied.In this study,the optimal reaction conditions were determined as follows:the ratio of EPS to sodium selenite was 1∶1,reaction at 37℃for 6 h,pH 4.The characteristic peak of SeNPs appeared at 250~300 nm in full wavelength scanning,indicating the successful compounding of sodium selenite on EPS.SeNPs had higher apparent viscosity than EPS,which led to a more desirable compatibility during industrial application.Cytotoxicity experiments showed that SeNPs did not inhibit the growth of mouse macrophage RAW 264.7 at the concentration of 0~3 mg·mL^(-1),indicating that SeNPs was not toxic within this concentration range.
基金financially supported by the“Young reserve talents”special program from Harbin University of Commerce(2019CX31)Doctoral Scientific Research Start-up foundation from Harbin University of Commerce(2019DS098)Graduate innovation fund project of Harbin University of Commerce(YJSCX2019–615HSD).The authors are grateful to supports.
文摘Type 2 diabetes(T2D)is a complex metabolic disease characterized by insulin resistance and chronic inflammation.This study explored the effects of sweet corn cob selenium polysaccharide(SeSCP),a complex of selenium and sweet corn cob polysaccharide,on T2D.SeSCP demonstrated significant hypoglycemic effects.This investigation focused on the impact of SeSCP on the intestinal barrier,inflammation,and microbiota in streptozotocin(STZ)-induced T2D mice.These findings revealed that SeSCP effectively reduced blood glucose levels and insulin resistance,decreased intestinal barrier permeability,and modulated the LPS/IκBα/NFκB pathway to reduce inflammatory factor production.Additionally,SeSCP corrected intestinal flora imbalances,increasing the relative abundance of Firmicutes in T2D mice.Furthermore,SeSCP enhanced carbohydrate and lipid metabolism in T2D mice,as indicated by Kyoto Encyclopedia of Genes and Genomes(KEGG)analysis,demonstrating its potential to alleviate T2D through intestinal modulation.
基金supported by the National Natural Science Foundation of China(32172259,32472381)the Key Research and Development Project of Henan Province(231111111800)the Food Engineering Technology Research Center/Key Laboratory of Henan Province(GO202318).
文摘Selenium-enriched polysaccharides refer to polysaccharides that contain selenium,which have emerged as a significant area of research in recent years due to the bioactivities associated with both polysaccharides and selenium.In this study,we prepared Spirulina platensis polysaccharides(SPP)and selenium-enriched SPP(SeSPP),and assessed their structural properties and hypoglycaemic functions both in vitro and in vivo.The re-sults of the structural characterization indicated that SPP was successfully selenized.The interaction between SPP and selenium primarily occurred in the forms of Se-O-C and O-Se-O through covalent interactions.The molecular weight of SeSPP was found to be more concentrated compared to SPP,and its microscopic morphology exhibited greater complexity,including both rod-shaped and spherical particles.It was determined that SPP and SeSPP were mainly composed of glucose,rhamnose,and fucose.In vitro assays demonstrated that SeSPP exhibited significantly enhanced hypoglycaemic and antioxidant properties compared to SPP.In an in vivo model of streptozotocin-and high-fat diet-induced type 2 diabetes mice,SeSPP improved hypoglycaemic activity by reversing body weight,blood glucose levels,glucose tolerance,insulin resistance,lipid levels,hepatic oxidative stress levels,as well as liver and pancreatic morphology and function.Consequently,SeSPP may serve as a se-lenium supplement that could enhance hypoglycaemic effects.
