Wound dressings with tissue adhesion,good mechanical,antioxidant and anti-inflammatory performance are urgently needed.In this work,we present a multifunctional selenium nanoparticles(SeNPs)/citric acid/gelatin/hydrox...Wound dressings with tissue adhesion,good mechanical,antioxidant and anti-inflammatory performance are urgently needed.In this work,we present a multifunctional selenium nanoparticles(SeNPs)/citric acid/gelatin/hydroxysuccinimide-grafted polyacrylic acid nanocomposite hydrogel adhesive(SCA) specifically designed for wound healing applications.The SCA was prepared via a one-pot processing,where SeNPs synthesized via chemical reduction were incorporated.These SeNPs not only endowed SCA with robust wet adhesion ability,excellent stretchability,and skin-matched elasticity modulus by serving as a physical crosslinker to modulate swelling equilibrium and molecular slippage,but also enhanced the biocompatibility and free radical scavenging capacity of SCA.Furthermore,in vivo evaluation of full-thickness cutaneous defects of rats revealed that SCA effectively reduced inflammation,promoted wound closure,and increased collagen deposition.All these results demonstrated that the developed SCA offers a promising therapeutic strategy for wound healing applications.展开更多
Selenium nanoparticles(SeNPs)are increasingly recognized for their exceptional antibacterial properties.This study aimed to develop a green,safe,and efficient method for the biosynthesis of SeNPs using the fungus Euro...Selenium nanoparticles(SeNPs)are increasingly recognized for their exceptional antibacterial properties.This study aimed to develop a green,safe,and efficient method for the biosynthesis of SeNPs using the fungus Eurotium cristatum,a novel approach in SeNP synthesis.The process yielded(36.40±4.22)mg of SeNPs per liter of 1.2 mmol/L sodium selenite supplementation.These SeNPs exhibited an average diameter of 231.7 nm and a negative charge,and they remained stable when stored at 4℃.Ultraviolet and visible spectrophotometry revealed a maximum absorption peak at 212 nm,suggesting effective nanoparticle formation.Fourier transform infrared spectrometry indicated that proteins and carbohydrates in the mycelium contributed to the SeNP synthesis.Concentrations of SeNPs below 50μg Se/mL did not exhibit cytotoxic effects on the growth and proliferation of human hepatocyte L-02 cells.The minimum inhibitory concentration of SeNPs was found to be 2 mg/mL against both methicillin-resistant Staphylococcus aureus(Gram-positive)and Escherichia coli(Gram-negative).The SeNPs compromised the cellular integrity of test strains,causing leakage of intracellular contents and disruption of the oxidative stress system,leading to irreversible damage.Our results demonstrate the potential of SeNPs biosynthesized by E.cristatum to act as effective antibacterial agents,signifying a novel and promising approach to developing natural antimicrobial solutions.展开更多
Selenium nanoparticles were prepared by a reverse microemulsion system. Sodium selenosulfate was used as selenium source. The results showed that hydrochloric acid concentration and reaction temperature had great infl...Selenium nanoparticles were prepared by a reverse microemulsion system. Sodium selenosulfate was used as selenium source. The results showed that hydrochloric acid concentration and reaction temperature had great influence on the morphology of products. The crystalline selenium nanowires and amorphous selenium nanorods were obtained in given condition.展开更多
The application of selenium nanoparticles(SeNPs)as nanofertilizers may lead to the release of SeNPs into aquatic systems.However,the environmental behavior of SeNPs is rarely studied.In this study,using alginate-coate...The application of selenium nanoparticles(SeNPs)as nanofertilizers may lead to the release of SeNPs into aquatic systems.However,the environmental behavior of SeNPs is rarely studied.In this study,using alginate-coated SeNPs(Alg-SeNPs)and polyvinyl alcohol-coated SeNPs(PVA-SeNPs)as models,we systematically investigated the aggregation and stability of SeNPs under various water conditions.PVA-SeNPs were highly stable in mono-and polyvalent electrolytes,probably due to the strong steric hindrance of the capping agent.Alg-SeNPs only suffered from a limited increase in size,even at 2500 mmol/L NaCl and 200 mmol/L MgCl_(2),while they underwent apparent aggregation in CaCl_(2) and LaCl_(3) solutions.The binding of Ca^(2+) and La^(3+) with the guluronic acid part in alginate induced the formation of cross-linking aggregates.Natural organic matter enhanced the stability of Alg-SeNPs in monovalent electrolytes,while accelerated the attachment of Alg-SeNPs in polyvalent electrolytes,due to the cation bridge effects.The long-term stability of SeNPs in natural water showed that the aggregation sizes of Alg-SeNPs and PVA-SeNPs increased to several hundreds of nanometers or above 10μm after 30 days,implying that SeNPs may be suspended in the water column or further settle down,depending on the surrounding water chemistry.The study may contribute to the deep insight into the fate and mobility of SeNPs in the aquatic environment.The varying fate of SeNPs in different natural waters also suggests that the risks of SeNPs to organisms living in diverse depths in the aquatic compartment should be concerned.展开更多
AH_(2)O_(2)-induced oxidative stress injury cell model was established to investigate the antioxidant effect of nanoselenium on mouse spermatocyte lines and the regulation mechanism of the expression level and activit...AH_(2)O_(2)-induced oxidative stress injury cell model was established to investigate the antioxidant effect of nanoselenium on mouse spermatocyte lines and the regulation mechanism of the expression level and activity of seleniumcontaining antioxidant enzymes induced by oxidative stress.A safe and effective nano-drug system of functionalized selenium-containing nanoparticles(SeNPs)was developed with lentinan(LNT)(SeNPs@LNT).Mice spermatocyte line GC2-spg cells were treated with SeNPs@LNT(1,2,4,8,16,32μM)for 24-72 h to evaluate the cytotoxicity of selenium.GC2-spg cells were randomly divided into the following groups:control,hydrogen peroxide(H_(2)O_(2)),SeNPs@LNT,and H_(2)O_(2)+SeNPs@LNT groups.H_(2)O_(2)+SeNPs@LNT group was pretreated with SeNPs@LNT 4μM for 12 h,followed by H_(2)O_(2)600μM for 8 h.The cell viability decreased in the H_(2)O_(2) group and increased significantly in the SeNPs@LNT group.Compared with the H_(2)O_(2) group,the SeNPs@LNT+H_(2)O_(2) group exhibited obvious red fluorescence,indicating a higher level of mitochondrial membrane potential.The content of intracellular reactive oxygen species(ROS)in the SeNPs@LNT group reduced significantly,and the intensity of green fluorescence in the SeNPs@LNT+H_(2)O_(2) group decreased significantly compared with the H_(2)O_(2) group,indicating the inhibitory effect of SeNPs@LNT on the generation of ROS-induced oxidative stress.The activity of GPx and SOD increased significantly in the SeNPs@LNT group.The expression of p53 decreased significantly under the intervention of nano-selenium,and GPx1 expression increased.In the oxidative stress group,the expressions of DNA damage-related proteins and apoptosis-related proteins were higher than those in other groups.Thus,SeNPs@LNT can promote GC2-spg cell proliferation,improve GPx and SOD activities,remove intracellular ROS,and reduce mitochondrial damage and functional abnormalities caused by oxidative stress by regulating the ERK and p53 protein levels.SeNPs@LNT has good biological activity and antioxidant effect,which can be used to protect the male reproductive system from oxidative stress.展开更多
Background:Nano-selenium has been widely used in antiviral and anticancer therapy,and has the advantages of good targeting and low toxicity.For the first time,we combined male reproduction with nano-selenium to invest...Background:Nano-selenium has been widely used in antiviral and anticancer therapy,and has the advantages of good targeting and low toxicity.For the first time,we combined male reproduction with nano-selenium to investigate its antioxidant effect.This study investigated the protective effect of lentinan functionalized selenium nanoparticles on oxidative stress injury of the hydrogen peroxide(H_(2)O_(2))-induced Leydig cell line,TM3.Methods:The suitable concentration of nano-selenium treatment to promote cell proliferation was also discussed.The concentration of 4μM could significantly promote the growth of TM3 cells.Oxidative stress damage was caused using an 800μM concentration of hydrogen peroxide.The cells were divided into four groups:normal control group,oxidative stress treatment group,H_(2)O_(2)+SeNPs@LNT group,and SeNPs@LNT group.The H_(2)O_(2)+SeNPs@LNT group was pretreated with 4μM of SeNPs@LNT for 12 h,followed by 800μM of H_(2)O_(2)for 8 h.Results:Nano-selenium could significantly promote the proliferation and viability of TM3 cells.SeNPs@LNT treatment increased the level of mitochondrial membrane potential in normal cells and slowed down the decline in mitochondrial membrane potential level caused by oxidative stress injury.In addition,the increase in reactive oxygen species caused by oxidative stress was inhibited by SeNPs@LNT treatment.The apoptosis of TM3 cells was detected,and SeNPs@LNT alleviated the necrosis and apoptosis of TM3 cells induced by H_(2)O_(2).Nano-selenium plays a protective role against oxidative H_(2)O_(2)-induced stress injury in TM3 cells through the changes in the Janus kinase 2/signal transducer and activator of transcription 3 signaling pathway and P53 pathway,and the expression levels of other related proteins,protein kinase B(AKT)and C3.Conclusion:SeNPs@LNT exhibited good biological activity and antioxidant effect and can thus be used to protect the male reproductive system from oxidative stress.展开更多
<span style="font-family:Verdana;">A simple method for assessment of the toxicity and antidote effect of selenium nanoparticles with </span><i><i><span style="font-family:Verd...<span style="font-family:Verdana;">A simple method for assessment of the toxicity and antidote effect of selenium nanoparticles with </span><i><i><span style="font-family:Verdana;">Paramecium</span></i><span> <i><span style="font-family:Verdana;">caudatum</span></i><span style="font-family:Verdana;"></span></span></i><span style="font-family:Verdana;"> is presented. Light microscopy in combination with computerized video tracking is employed for </span><span style="font-family:Verdana;">the </span><span style="font-family:Verdana;">determination of </span><span style="font-family:Verdana;">the </span><span style="font-family:;" "=""><span style="font-family:Verdana;">survival time of <i></i></span><i><i><span style="font-family:Verdana;">P.</span></i><span> <i><span style="font-family:Verdana;">caudatum</span></i><span style="font-family:Verdana;"></span></span></i></span><span style="font-family:Verdana;">. Up to 800 mg/L, selenium nanoparticles are not acutely toxic. </span><span style="font-family:Verdana;">With</span><span style="font-family:;" "=""><span style="font-family:Verdana;"> respect to a potential antidote effect, the lethality of silver nanoparticles, silver nitrate, sodium hydrogen selenite, and sodium selenite to <i></i></span><i><i><span style="font-family:Verdana;">P.</span></i><span> <i><span style="font-family:Verdana;">caudatum</span></i><span style="font-family:Verdana;"></span></span></i></span><span style="font-family:Verdana;"> was decreased and survival time was extend</span><span style="font-family:Verdana;">ed upon pre-treatment with selenium nanoparticles. Taken together, these findings suggest that administration of selenium nanoparticles attenuates</span><span style="font-family:Verdana;"> exposure </span><span style="font-family:Verdana;">to</span><span style="font-family:Verdana;"> toxicants. Selenium nanoparticles could be a good functional additive for food management in animals.</span>展开更多
Selenium(Se)plays an important role in the development and treatment of lung cancer,yet its specific mechanisms remain elusive.Lower Se level in serum was noted in lung cancer patients compared to normal controls.Ther...Selenium(Se)plays an important role in the development and treatment of lung cancer,yet its specific mechanisms remain elusive.Lower Se level in serum was noted in lung cancer patients compared to normal controls.Therefore,developing effective therapeutic adjuvants containing Se might benefit the treatment of lung cancer patients.This study aimed to investigate the association between Se and the chemotherapeutic efficacy of lung cancer.Lentinan-modified selenium nanoparticles(LET-Se NPs)were created to develop and verify the effectiveness of Se containing adjuvant applied with pemetrexed on lung cancer cells.A synergistic effect was observed between LET-Se NPs and pemetrexed in vitro.The combination of LET-Se NPs and pemetrexed could induce reactive oxygen species overproduction,mitochondrial dysfunction and DNA damage,ultimately leading to cancer cell apoptosis.It is implied that LET-Se NPs might be a promising sensitizer to pemetrexed chemotherapy and could potentially enhance chemotherapy efficiency in non-small cell lung cancer.展开更多
Objective: The chemo-preventative and therapeutic properties of selenium nanoparticles(Se NPs) have been documented over recent decades and suggest the potential uses of Se NPs in medicine. Biogenic Se NPs have higher...Objective: The chemo-preventative and therapeutic properties of selenium nanoparticles(Se NPs) have been documented over recent decades and suggest the potential uses of Se NPs in medicine. Biogenic Se NPs have higher biocompatibility and stability than chemically synthesized nanoparticles, which enhances their medical applications, especially in the field of cancer therapy. This study evaluated the potential of green-synthetized Se NPs by using berberine(Ber) as an antitumor agent and elucidated the mechanism by which these molecules combat Ehrlich solid tumors(ESTs).Methods: Se NPs containing Ber(Se NPs-Ber) were synthesized using Ber and Na2 Se O3 and characterized with Fourier transform infrared spectroscopy. Sixty male Swiss albino mice were then acclimatized for one week, injected with Ehrlich ascites tumor cells, and divided into four groups: EST, EST + cisplatin(5 mg/kg), EST + Ber(20 mg/kg), and EST + Se NPs-Ber(0.5 mg/kg). At the end of a 16-day observation period, 12 mice from each group were euthanized to analyze differences in the body weight, tumor size,gene expression, and oxidative stress markers in the four groups. Three mice from each group were kept alive to compare the survival rates.Results: Treatment with Se NPs-Ber significantly improved the survival rate and decreased the body weight and tumor size, compared to the EST group. Se NPs-Ber reduced oxidative stress in tumor tissue,as indicated by a decrease in the lipid peroxidation and nitric oxide levels and an increase in the glutathione levels. Moreover, Se NPs-Ber activated an apoptotic cascade in the tumor cells by downregulating the B-cell lymphoma 2(Bcl-2) expression rate and upregulating the Bcl-2-associated X protein and caspase-3 expression rates. Se NPs-Ber also considerably improved the histopathological alterations in the developed tumor tissue, compared to the EST group.Conclusion: Our study provides a new insight into the potential role of green-synthesized Se NPs by using Ber as a promising anticancer agent, these molecules could be used alone or as supplementary medication during chemotherapy.展开更多
Selenium nanoparticles(SeNPs)have been demonstrated potential for use in diseases associated with oxidative stress.Functionalized SeNPs with lower toxicity and higher biocompatibility could bring better therapeutic ac...Selenium nanoparticles(SeNPs)have been demonstrated potential for use in diseases associated with oxidative stress.Functionalized SeNPs with lower toxicity and higher biocompatibility could bring better therapeutic activity and clinical application value.Herein,this work was conducted to investigate the protective effect of Pleurotus tuber-regium polysaccharide-protein complex funtionnalized SeNPs(PTR-SeNPs)against acetaminophen(APAP)-induced oxidative injure in HepG2 cells and C57BL/6J mouse liver.Further elucidation of the underlying molecular mechanism,in particular their modulation of Nrf2 signaling pathway was also performed.The results showed that PTR-SeNPs could significantly ameliorate APAP-induced oxidative injury as evidenced by a range of biochemical analysis,histopathological examination and immunoblotting study.PTR-SeNPs could hosphorylate and activate PKCδ,depress Keap1,and increase nuclear accumulation of Nrf2,resulting in upregulation of GCLC,GCLM,HO-1 and NQO-1 expression.Besides,PTR-SeNPs suppressed the biotransformation of APAP to generate intracellular ROS through CYP 2E1 inhibition,restoring the mitochondrial morphology.Furthermore,the protective effect of PTR-SeNPs against APAP induced hepatotoxicity was weakened as Nrf2 was depleted in vivo,indicating the pivotal role of Nrf2 signaling pathway in PTR-SeNPs mediated hepatoprotective efficacy.Being a potential hepatic protectant,PTR-SeNPs could serve as a new source of selenium supplement for health-promoting and biomedical applications.展开更多
Nowadays,doctors and nutritionists recommend individuals incorporate selenium-rich foods such as nuts,cereals,and mushrooms into their regular diet to maintain fitness and overall health.Selenium nanoparticles(SeNPs)e...Nowadays,doctors and nutritionists recommend individuals incorporate selenium-rich foods such as nuts,cereals,and mushrooms into their regular diet to maintain fitness and overall health.Selenium nanoparticles(SeNPs)exhibit strong chemopreventive capabilities.The anticipations for SeNPs with enhanced and tunable bioactive activities have led to a keen interest in phytofabrication.In this study,the aqueous extract of Clerodendron phlomidis plant leaves was utilized for the synthesis of SeNPs.In traditional Indian medicine,this plant extract is recognized as a significant anti-diabetic agent.The flavonoids tetrahydroxylflavone,7-hydroxyflavanone,and 6,4’-dimethyl-7-acetoxy-scutellarein present in this plant leaf extract demonstrate excellent anticancer activity.These secondary metabolites exhibit the ability to reduce sodium selenite into SeNPs.At a concentration of 13μg/mL,the synthesized SeNPs effectively inhibited the proliferation of the HepG2 cell line.The results suggest that the SeNPs possess promising anti-cancer potential against liver cancer and can be considered as a therapeutic agent for liver cancer treatment.Additionally,the cell cycle arrest induced by SeNPs was further confirmed by the fluorescence-activated cell sorting(FACS)method,indicating that SeNPs could efficiently differentiate cancer cells from normal cells.Notably,it showed a significant improvement in diethylnitrosamine(DEN)-induced Swiss Wistar rat groups.This scientific investigation highlights the high anti-cancer potential of SeNPs,positioning them as a promising therapeutic agent for liver cancer treatment.展开更多
The well-distributed, stable selenium nanoparticles (10 nm) with good adhesive ability and biocompatibility were successfully synthesized by using the template of chitosan cross-linked with glutaradehyde. The resulti...The well-distributed, stable selenium nanoparticles (10 nm) with good adhesive ability and biocompatibility were successfully synthesized by using the template of chitosan cross-linked with glutaradehyde. The resulting selenium nanoparticles were used as a new carrier for horseradish peroxidase to construct H2O2 biosensors with good performances.展开更多
In this study,sesame leaf mucilage(SmE)was utilized as a natural reducing and stabilizing agent for the green synthesis of selenium nanoparticles(SeNPs).The resulting SmE/SeNPs were then incorporated with chitosan(CT)...In this study,sesame leaf mucilage(SmE)was utilized as a natural reducing and stabilizing agent for the green synthesis of selenium nanoparticles(SeNPs).The resulting SmE/SeNPs were then incorporated with chitosan(CT)to fabricate nanocomposites(CT/SmE/SeNPs)in three ratios:1:2,1:1,and 2:1.The composites were characterized for particle size and morphology using transmission electron microscopy.SmE/SeNPs exhibited a well-dispersed spherical structure with an average particle size of 14.73 nm.Among the formulations,the 1:1 CT:SmE/SeNPs nanocomposite showed the most potent in-vitro antifungal activity against Penicillium commune and Penicillium crustosum,with an average size of 108.65 nm.When applied at 0.1%concentration to soft cheese samples inoculated with Penicillium spp.,this nanocomposite significantly inhibited fungal growth and extended product shelf life.This study presents a novel,green-synthesized antifungal nanocomposite combining chitosan and SeNPs stabilized by sesame leaf mucilage-a byproduct not commonly explored for this purpose.The developed nanocomposite offers a sustainable,effective,and food-safe solution to control fungal spoilage in dairy products,particularly soft cheese.展开更多
Cranberry polyphenols(CPs)were obtained via ultrasound-assisted extraction and purification on a macroporous resin X-5 column.A green synthesis method of selenium nanoparticles(SeNPs)using CPs as reducing agents was t...Cranberry polyphenols(CPs)were obtained via ultrasound-assisted extraction and purification on a macroporous resin X-5 column.A green synthesis method of selenium nanoparticles(SeNPs)using CPs as reducing agents was then developed.Two types of SeNPs(CP-SeNPs1 and CP-SeNPs2)were successfully constructed and character-ized.The spherical particles were well-dispersed on the polyphenol templates and the addition of the poly-phenols reduced the aggregation of the nanoparticles.Both CP-SeNPs1 and CP-SeNPs2,with average particle sizes of 85.62±0.11 nm and 107.97±0.12 nm,respectively,demonstrated radical-scavenging activities and protective effects on erythrocyte hemolysis.CP-SeNPs2 possessed more significant antioxidant activity,as evi-denced by its higher radical-scavenging rate and greater enhancement of the erythrocyte antioxidant state compared to those of CP-SeNPs1.This study provides a new application of CPs and confirms their great potential in stabilizing SeNPs.展开更多
Dendrobium officinale polysaccharides(DOP),as a natural active ingredient,has exhibited strong immunomodulatory and antioxidant effects.Selenium nanoparticles(SeNPs)also possess various biological activities,but the u...Dendrobium officinale polysaccharides(DOP),as a natural active ingredient,has exhibited strong immunomodulatory and antioxidant effects.Selenium nanoparticles(SeNPs)also possess various biological activities,but the unstable properties limit their applications.This research focused on modification of SeNPs by DOP to form stable complexes(DOP-SeNPs),and the analysis of structural characterization and gut microbiota-mediated immune regulation mechanisms.The results showed that DOP-SeNPs were prepared through hydrogen bond interaction and presented uniform particle size distribution(average particle size of about 100 nm)and good stability.Animal experiments demonstrated that DOP-SeNPs significantly protected the immune organs in cyclophosphamide-induced immune injury mice.Besides,DOP-SeNPs effectively regulated the gut microbiota diversity including promoting the enrichments of probiotics such as Lactobacillus and Lachnospiraceae NK4Al36 group compared with model group,and improved the lymphocyte subsets proportions through the regulation of metabolic pathways including biosynthesis of various antibiotics,protein digestion and absorption,chemical carcinogenesis-DNA adducts.In conclusion,DOP-SeNPs presented stable structure characteristics and improved the immune functions of immune-injured mice by influencing the gut microbiota metabolism.These results will provide a certain theoretical basis and data support for the applications of DOP and the nano-selenium complex in functional food industry.展开更多
The controlled release of selenium nanoparticles(SeNPs)is pivotal significance in improving bioavailability and mitigating potential side effects.Three types of SeNPs,Selenium nanoparticles synthesized by chemical met...The controlled release of selenium nanoparticles(SeNPs)is pivotal significance in improving bioavailability and mitigating potential side effects.Three types of SeNPs,Selenium nanoparticles synthesized by chemical method(CSeNPs),extracellular polysaccharide-encapsulated selenium nanoparticles(EPS-SeNP),and selenium nanoparticles synthesized by Lactiplantibacillus plantarum(LacSeNPs),were synthesized in this study using chemical reduction,polysaccharide-assisted,and microbial reduction methods,respectively.The selenium release kinetics of the nanoparticles were investigated in simulated gastrointestinal with five kinetics models.CSeNPs released 67.80% of selenium in simulated gastric fluids(SGF)and,depicting a Brownian motion fraction of 81.0.EPSSeNPs released 77.38% of selenium in SGF while only releasing 16.28% in simulated intestinal fluids(SIF).The release process was primarily affected by changes in the nanoparticle structure after Super Case II Transport in SGF and the zero-order model in SIF.The zero-order model provided the best fit for releasing LacSeNP profiles in the SGF.In SIF,the release mechanism of LacSeNPs was regulated by combination of diffusion and carrier swelling or erosion,leading to non-Fickian diffusion.Therefore,synthetic nanoparticle methods determine their structures and compositions,leading to differences in their release kinetics and release mechanisms.Notably,the release of LacSeNPs in the SGF aligns with the absorption patterns of selenium within the human body.The current study elucidates the potential application advantages of SeNPs synthesized using microbial means as selenium-enriched supplements.展开更多
Compared with conventional selenium forms,microbially biosynthesis selenium nanoparticles(SeNPs)have garnered considerable interest owing to their superior biocompatibility and bioavailability,holding significant pote...Compared with conventional selenium forms,microbially biosynthesis selenium nanoparticles(SeNPs)have garnered considerable interest owing to their superior biocompatibility and bioavailability,holding significant potential for applications in technological and health-related fields.This study establishes a sustainable bio-logical platform using the marine yeast Scheffersomyces spartinae 12SS-9 for efficient SeNP biosynthesis via selenite bioreduction.The strain demonstrated excellent selenite tolerance and efficiently converted it into SeNPs,achieving a maximum yield of 17.30±1.14 mg/g dry cell weight under 1 mmol/L Na_(2)SeO_(3) stress.The biosynthesized SeNPs were spherical,amorphous,and polydisperse,with stability conferred by surface-bound biomolecules.In vitro assays revealed significant concentration-dependent antioxidant activities,including effective scavenging of multiple free radicals and inhibition of lipid peroxidation.Furthermore,S.spartinae-derived SeNPs demonstrated a markedly improved safety profile compared to inorganic selenite in two fish cell lines:the Epinephelus coioides grouper intestinal epithelial cell line(ECGI)and Epithelioma papulosum cyprini cell line(EPC),and provided robust protection against H_(2)O_(2)-induced oxidative stress by restoring antioxidant enzyme activities and reducing intracellular levels of reactive oxygen species(ROS)and malondialdehyde(MDA).This work highlights S.spartinae 12SS-9 as a highly efficient and eco-friendly microbial cell factory for SeNP production,offering a promising,safe,and scalable selenium supplement for aquaculture.Additionally,preliminary insights into the mechanisms underlying selenite bioreduction,SeNP formation,and extracellular release in this marine yeast are proposed.展开更多
Inflammation is a fundamental response process to injury,which is coordinated by various inflammatory mediators,including reactive oxygen species,inflammatory immune cells and cytokines.Excessive inflammatory response...Inflammation is a fundamental response process to injury,which is coordinated by various inflammatory mediators,including reactive oxygen species,inflammatory immune cells and cytokines.Excessive inflammatory responses can drive the development of numerous diseases,making modulation of inflammatory responses a crucial strategy in the treatment of inflammatory diseases.Selenium(Se),an essential trace element for human health,plays a crucial role in alleviating severe inflammatory diseases by scavenging free radicals,achieving antioxidant effects and modulating the innate and adaptive immune systems in the inflammatory microenvironment.Among the different forms of Se element,Selenium nanoparticles(SeNPs)stand out for their low toxicity and high utilization rate,offering them unique advantages in the fields of medicine and materials science.In addition,functional modification of SeNPs enhances their biological activities in modulating the process of inflammatory diseases.In this review,the construction of various types of SeNPs are firstly discussed.Then,we review the diagnosing effects of modified SeNPs and highlight their biomedical applications in the treatment of inflammation.Finally,we summarize the limitations in the current research of SeNPs as well as the major challenges in clinical transformation,and anticipate the application prospects of new functionalized SeNPs in the treatment of inflammatory diseases.展开更多
Nanotechnologies have been exploited to develop safe and effective medicines and pharmaceuticals.In the present study,a novel functional nanomedicine constructed from a bioactive polysaccharide and selenium nanoparfic...Nanotechnologies have been exploited to develop safe and effective medicines and pharmaceuticals.In the present study,a novel functional nanomedicine constructed from a bioactive polysaccharide and selenium nanoparficles （SeNPs） was developed.A highly-branched [3-（1→3）-D-glucan （AF1） with high anti-tumor activity was used to self-assemble hollow nanofibers with an apparent average diameter of 92 nm;Se nanopartides were synthesized via the reduction of sodium selenite.The results of light scattering,transmission electron microscopy,and X-ray diffraction demonstrated that the spherical SeNPs with a mean diameter of 46 nm were entrapped in the cavities of the AF1 hollow nanofibers through the formation of Se-O bonds between SeNPs and AF1,leading to the good dispersion and high stability in water for over 16 months.In vitro and in vivo assays indicated that the AF1-Se nanocomposite had higher anti-tumor activities against breast cancer.Furthermore,AF1-Se displayed a broad-spectrum inhibition against human cancers with low half maximal inhibitory concentration （IC50） values and low toxicity to normal cells.Particularly,the inhibition ratio of AF1-Se against MCF-7 cancer cells reached 75% at a concentration of 200 μg.mL-1 with 29 μM Se content,much higher than that by treatment with AF1 alone,suggesting a strong synergic effect and nano impact.Overall,we developed a method for increasing the stability,anti-tumor activity,and safety of SeNPs by wrapping with bioactive polysaccharides.展开更多
Human adenovirus(HAdV)can cause severe respiratory infections in immunocompromised patients,but its clinical treatment is seriously limited by side effects of drugs such as poor efficacy,low bioavailability and severe...Human adenovirus(HAdV)can cause severe respiratory infections in immunocompromised patients,but its clinical treatment is seriously limited by side effects of drugs such as poor efficacy,low bioavailability and severe nephrotoxicity.Trace element selenium(Se)has been found will affect the disease progression of pneumonia,but its antivirus efficacy could be improved by speciation optimization.Therefore,herein we performed anti-HAdV effects of different Se speciation and found that lentinan(LNT)-decorated selenium nanoparticles(SeNPs)exhibited low cytotoxicity and excellent anti-HAdV antiviral activity.Furthermore,SeNPs@LNT reduced the HAdV infection-induced mitochondrial damage and excessive production of reactive oxygen species(ROS).It was also involved in the repair of host cell DNA damage and inhibition of viral DNA replication.SeNPs@LNT inhibited HAdV-induced apoptosis mainly by modulating the p53/Bcl-2 apoptosis signaling pathway.In vivo,SeNPs@LNT replenished Se by targeting the infected site through the circulatory system and was involved in the synthesis of Glutathione peroxidase 1(GPx1).More importantly,GPx1 played an antioxidant and immunomodulatory role in alleviating HAdV-induced inflammatory cytokine storm and alleviating adenovirus pneumonia in Se-deficient mice.Collectively,this study provides a Se speciation of SeNPs@LNT with anti-HAdV activity,and demonstrate that SeNPs@LNT is a promising pharmaceutical candidate for the treatment of HAdV.展开更多
基金supported by National Natural Science Foundation of China(No.52403042)China Postdoctoral Science Foundation(No.2023M742472)。
文摘Wound dressings with tissue adhesion,good mechanical,antioxidant and anti-inflammatory performance are urgently needed.In this work,we present a multifunctional selenium nanoparticles(SeNPs)/citric acid/gelatin/hydroxysuccinimide-grafted polyacrylic acid nanocomposite hydrogel adhesive(SCA) specifically designed for wound healing applications.The SCA was prepared via a one-pot processing,where SeNPs synthesized via chemical reduction were incorporated.These SeNPs not only endowed SCA with robust wet adhesion ability,excellent stretchability,and skin-matched elasticity modulus by serving as a physical crosslinker to modulate swelling equilibrium and molecular slippage,but also enhanced the biocompatibility and free radical scavenging capacity of SCA.Furthermore,in vivo evaluation of full-thickness cutaneous defects of rats revealed that SCA effectively reduced inflammation,promoted wound closure,and increased collagen deposition.All these results demonstrated that the developed SCA offers a promising therapeutic strategy for wound healing applications.
基金supported by Key Research and Development Program of Shaanxi(2024NC-GJHX-12)the National Natural Science Foundation of China(32172301).
文摘Selenium nanoparticles(SeNPs)are increasingly recognized for their exceptional antibacterial properties.This study aimed to develop a green,safe,and efficient method for the biosynthesis of SeNPs using the fungus Eurotium cristatum,a novel approach in SeNP synthesis.The process yielded(36.40±4.22)mg of SeNPs per liter of 1.2 mmol/L sodium selenite supplementation.These SeNPs exhibited an average diameter of 231.7 nm and a negative charge,and they remained stable when stored at 4℃.Ultraviolet and visible spectrophotometry revealed a maximum absorption peak at 212 nm,suggesting effective nanoparticle formation.Fourier transform infrared spectrometry indicated that proteins and carbohydrates in the mycelium contributed to the SeNP synthesis.Concentrations of SeNPs below 50μg Se/mL did not exhibit cytotoxic effects on the growth and proliferation of human hepatocyte L-02 cells.The minimum inhibitory concentration of SeNPs was found to be 2 mg/mL against both methicillin-resistant Staphylococcus aureus(Gram-positive)and Escherichia coli(Gram-negative).The SeNPs compromised the cellular integrity of test strains,causing leakage of intracellular contents and disruption of the oxidative stress system,leading to irreversible damage.Our results demonstrate the potential of SeNPs biosynthesized by E.cristatum to act as effective antibacterial agents,signifying a novel and promising approach to developing natural antimicrobial solutions.
文摘Selenium nanoparticles were prepared by a reverse microemulsion system. Sodium selenosulfate was used as selenium source. The results showed that hydrochloric acid concentration and reaction temperature had great influence on the morphology of products. The crystalline selenium nanowires and amorphous selenium nanorods were obtained in given condition.
基金supported by the National Natural Science Foundation of China(Nos.22076198,42192571,and 21827815).
文摘The application of selenium nanoparticles(SeNPs)as nanofertilizers may lead to the release of SeNPs into aquatic systems.However,the environmental behavior of SeNPs is rarely studied.In this study,using alginate-coated SeNPs(Alg-SeNPs)and polyvinyl alcohol-coated SeNPs(PVA-SeNPs)as models,we systematically investigated the aggregation and stability of SeNPs under various water conditions.PVA-SeNPs were highly stable in mono-and polyvalent electrolytes,probably due to the strong steric hindrance of the capping agent.Alg-SeNPs only suffered from a limited increase in size,even at 2500 mmol/L NaCl and 200 mmol/L MgCl_(2),while they underwent apparent aggregation in CaCl_(2) and LaCl_(3) solutions.The binding of Ca^(2+) and La^(3+) with the guluronic acid part in alginate induced the formation of cross-linking aggregates.Natural organic matter enhanced the stability of Alg-SeNPs in monovalent electrolytes,while accelerated the attachment of Alg-SeNPs in polyvalent electrolytes,due to the cation bridge effects.The long-term stability of SeNPs in natural water showed that the aggregation sizes of Alg-SeNPs and PVA-SeNPs increased to several hundreds of nanometers or above 10μm after 30 days,implying that SeNPs may be suspended in the water column or further settle down,depending on the surrounding water chemistry.The study may contribute to the deep insight into the fate and mobility of SeNPs in the aquatic environment.The varying fate of SeNPs in different natural waters also suggests that the risks of SeNPs to organisms living in diverse depths in the aquatic compartment should be concerned.
基金funded by the open Project of Guangdong Key Laboratory of Marine Materia(LMM2020-7)the science and technology projects in Guangzhou(202201020632,202201020638,202206010100,202201020655 and 202102010202)+2 种基金the Guangdong Natural Science Foundation(2020A1515110648)the Open Fund of Guangdong Provincial Key Laboratory of Functional Supramolecular Coordination Materials and Applications(2020A03)The Guangzhou Medical University Students’Science and Technology Innovation Project(02-408-2203-2079,2021AEK119).
文摘AH_(2)O_(2)-induced oxidative stress injury cell model was established to investigate the antioxidant effect of nanoselenium on mouse spermatocyte lines and the regulation mechanism of the expression level and activity of seleniumcontaining antioxidant enzymes induced by oxidative stress.A safe and effective nano-drug system of functionalized selenium-containing nanoparticles(SeNPs)was developed with lentinan(LNT)(SeNPs@LNT).Mice spermatocyte line GC2-spg cells were treated with SeNPs@LNT(1,2,4,8,16,32μM)for 24-72 h to evaluate the cytotoxicity of selenium.GC2-spg cells were randomly divided into the following groups:control,hydrogen peroxide(H_(2)O_(2)),SeNPs@LNT,and H_(2)O_(2)+SeNPs@LNT groups.H_(2)O_(2)+SeNPs@LNT group was pretreated with SeNPs@LNT 4μM for 12 h,followed by H_(2)O_(2)600μM for 8 h.The cell viability decreased in the H_(2)O_(2) group and increased significantly in the SeNPs@LNT group.Compared with the H_(2)O_(2) group,the SeNPs@LNT+H_(2)O_(2) group exhibited obvious red fluorescence,indicating a higher level of mitochondrial membrane potential.The content of intracellular reactive oxygen species(ROS)in the SeNPs@LNT group reduced significantly,and the intensity of green fluorescence in the SeNPs@LNT+H_(2)O_(2) group decreased significantly compared with the H_(2)O_(2) group,indicating the inhibitory effect of SeNPs@LNT on the generation of ROS-induced oxidative stress.The activity of GPx and SOD increased significantly in the SeNPs@LNT group.The expression of p53 decreased significantly under the intervention of nano-selenium,and GPx1 expression increased.In the oxidative stress group,the expressions of DNA damage-related proteins and apoptosis-related proteins were higher than those in other groups.Thus,SeNPs@LNT can promote GC2-spg cell proliferation,improve GPx and SOD activities,remove intracellular ROS,and reduce mitochondrial damage and functional abnormalities caused by oxidative stress by regulating the ERK and p53 protein levels.SeNPs@LNT has good biological activity and antioxidant effect,which can be used to protect the male reproductive system from oxidative stress.
基金Science and Technology Projects in Guangzhou(202201020632,202201020638,202206010100,202201020655,and 202102010202)the Guangdong Natural Science Foundation(2020A1515110648)+2 种基金the Open Project of Guangdong Key Laboratory of Marine Materia(LMM2020-7)the Open Fund of Guangdong Provincial Key Laboratory of Functional Supramolecular Coordination Materials and Applications(2020A03)the Guangzhou Medical University Students’Science and Technology Innovation Project(2021AEK119 and 02-408-2203-2079).
文摘Background:Nano-selenium has been widely used in antiviral and anticancer therapy,and has the advantages of good targeting and low toxicity.For the first time,we combined male reproduction with nano-selenium to investigate its antioxidant effect.This study investigated the protective effect of lentinan functionalized selenium nanoparticles on oxidative stress injury of the hydrogen peroxide(H_(2)O_(2))-induced Leydig cell line,TM3.Methods:The suitable concentration of nano-selenium treatment to promote cell proliferation was also discussed.The concentration of 4μM could significantly promote the growth of TM3 cells.Oxidative stress damage was caused using an 800μM concentration of hydrogen peroxide.The cells were divided into four groups:normal control group,oxidative stress treatment group,H_(2)O_(2)+SeNPs@LNT group,and SeNPs@LNT group.The H_(2)O_(2)+SeNPs@LNT group was pretreated with 4μM of SeNPs@LNT for 12 h,followed by 800μM of H_(2)O_(2)for 8 h.Results:Nano-selenium could significantly promote the proliferation and viability of TM3 cells.SeNPs@LNT treatment increased the level of mitochondrial membrane potential in normal cells and slowed down the decline in mitochondrial membrane potential level caused by oxidative stress injury.In addition,the increase in reactive oxygen species caused by oxidative stress was inhibited by SeNPs@LNT treatment.The apoptosis of TM3 cells was detected,and SeNPs@LNT alleviated the necrosis and apoptosis of TM3 cells induced by H_(2)O_(2).Nano-selenium plays a protective role against oxidative H_(2)O_(2)-induced stress injury in TM3 cells through the changes in the Janus kinase 2/signal transducer and activator of transcription 3 signaling pathway and P53 pathway,and the expression levels of other related proteins,protein kinase B(AKT)and C3.Conclusion:SeNPs@LNT exhibited good biological activity and antioxidant effect and can thus be used to protect the male reproductive system from oxidative stress.
文摘<span style="font-family:Verdana;">A simple method for assessment of the toxicity and antidote effect of selenium nanoparticles with </span><i><i><span style="font-family:Verdana;">Paramecium</span></i><span> <i><span style="font-family:Verdana;">caudatum</span></i><span style="font-family:Verdana;"></span></span></i><span style="font-family:Verdana;"> is presented. Light microscopy in combination with computerized video tracking is employed for </span><span style="font-family:Verdana;">the </span><span style="font-family:Verdana;">determination of </span><span style="font-family:Verdana;">the </span><span style="font-family:;" "=""><span style="font-family:Verdana;">survival time of <i></i></span><i><i><span style="font-family:Verdana;">P.</span></i><span> <i><span style="font-family:Verdana;">caudatum</span></i><span style="font-family:Verdana;"></span></span></i></span><span style="font-family:Verdana;">. Up to 800 mg/L, selenium nanoparticles are not acutely toxic. </span><span style="font-family:Verdana;">With</span><span style="font-family:;" "=""><span style="font-family:Verdana;"> respect to a potential antidote effect, the lethality of silver nanoparticles, silver nitrate, sodium hydrogen selenite, and sodium selenite to <i></i></span><i><i><span style="font-family:Verdana;">P.</span></i><span> <i><span style="font-family:Verdana;">caudatum</span></i><span style="font-family:Verdana;"></span></span></i></span><span style="font-family:Verdana;"> was decreased and survival time was extend</span><span style="font-family:Verdana;">ed upon pre-treatment with selenium nanoparticles. Taken together, these findings suggest that administration of selenium nanoparticles attenuates</span><span style="font-family:Verdana;"> exposure </span><span style="font-family:Verdana;">to</span><span style="font-family:Verdana;"> toxicants. Selenium nanoparticles could be a good functional additive for food management in animals.</span>
基金supported by National Natural Science Foundation for Distinguished Young Scholars(No.82225025)National Natural Science Foundation of China(Nos.21877049,32171296)。
文摘Selenium(Se)plays an important role in the development and treatment of lung cancer,yet its specific mechanisms remain elusive.Lower Se level in serum was noted in lung cancer patients compared to normal controls.Therefore,developing effective therapeutic adjuvants containing Se might benefit the treatment of lung cancer patients.This study aimed to investigate the association between Se and the chemotherapeutic efficacy of lung cancer.Lentinan-modified selenium nanoparticles(LET-Se NPs)were created to develop and verify the effectiveness of Se containing adjuvant applied with pemetrexed on lung cancer cells.A synergistic effect was observed between LET-Se NPs and pemetrexed in vitro.The combination of LET-Se NPs and pemetrexed could induce reactive oxygen species overproduction,mitochondrial dysfunction and DNA damage,ultimately leading to cancer cell apoptosis.It is implied that LET-Se NPs might be a promising sensitizer to pemetrexed chemotherapy and could potentially enhance chemotherapy efficiency in non-small cell lung cancer.
基金supported by the Scientific Research Deanship fund,University of Ha’il,Saudi Arabia(project number:RG-191334)。
文摘Objective: The chemo-preventative and therapeutic properties of selenium nanoparticles(Se NPs) have been documented over recent decades and suggest the potential uses of Se NPs in medicine. Biogenic Se NPs have higher biocompatibility and stability than chemically synthesized nanoparticles, which enhances their medical applications, especially in the field of cancer therapy. This study evaluated the potential of green-synthetized Se NPs by using berberine(Ber) as an antitumor agent and elucidated the mechanism by which these molecules combat Ehrlich solid tumors(ESTs).Methods: Se NPs containing Ber(Se NPs-Ber) were synthesized using Ber and Na2 Se O3 and characterized with Fourier transform infrared spectroscopy. Sixty male Swiss albino mice were then acclimatized for one week, injected with Ehrlich ascites tumor cells, and divided into four groups: EST, EST + cisplatin(5 mg/kg), EST + Ber(20 mg/kg), and EST + Se NPs-Ber(0.5 mg/kg). At the end of a 16-day observation period, 12 mice from each group were euthanized to analyze differences in the body weight, tumor size,gene expression, and oxidative stress markers in the four groups. Three mice from each group were kept alive to compare the survival rates.Results: Treatment with Se NPs-Ber significantly improved the survival rate and decreased the body weight and tumor size, compared to the EST group. Se NPs-Ber reduced oxidative stress in tumor tissue,as indicated by a decrease in the lipid peroxidation and nitric oxide levels and an increase in the glutathione levels. Moreover, Se NPs-Ber activated an apoptotic cascade in the tumor cells by downregulating the B-cell lymphoma 2(Bcl-2) expression rate and upregulating the Bcl-2-associated X protein and caspase-3 expression rates. Se NPs-Ber also considerably improved the histopathological alterations in the developed tumor tissue, compared to the EST group.Conclusion: Our study provides a new insight into the potential role of green-synthesized Se NPs by using Ber as a promising anticancer agent, these molecules could be used alone or as supplementary medication during chemotherapy.
基金financially supported by National Natural Science Foundation of China(81700524)Natural Science Foundation of Fujian Province(2022J01866)from Fujian Provincial Department of Science and Technology+1 种基金Key Project of Fujian University of Traditional Chinese Medicine(X2021019)Collaborative Innovation and Platform Establishment Project of Department of Science and Technology of Guangdong Province(2019A050520003)。
文摘Selenium nanoparticles(SeNPs)have been demonstrated potential for use in diseases associated with oxidative stress.Functionalized SeNPs with lower toxicity and higher biocompatibility could bring better therapeutic activity and clinical application value.Herein,this work was conducted to investigate the protective effect of Pleurotus tuber-regium polysaccharide-protein complex funtionnalized SeNPs(PTR-SeNPs)against acetaminophen(APAP)-induced oxidative injure in HepG2 cells and C57BL/6J mouse liver.Further elucidation of the underlying molecular mechanism,in particular their modulation of Nrf2 signaling pathway was also performed.The results showed that PTR-SeNPs could significantly ameliorate APAP-induced oxidative injury as evidenced by a range of biochemical analysis,histopathological examination and immunoblotting study.PTR-SeNPs could hosphorylate and activate PKCδ,depress Keap1,and increase nuclear accumulation of Nrf2,resulting in upregulation of GCLC,GCLM,HO-1 and NQO-1 expression.Besides,PTR-SeNPs suppressed the biotransformation of APAP to generate intracellular ROS through CYP 2E1 inhibition,restoring the mitochondrial morphology.Furthermore,the protective effect of PTR-SeNPs against APAP induced hepatotoxicity was weakened as Nrf2 was depleted in vivo,indicating the pivotal role of Nrf2 signaling pathway in PTR-SeNPs mediated hepatoprotective efficacy.Being a potential hepatic protectant,PTR-SeNPs could serve as a new source of selenium supplement for health-promoting and biomedical applications.
文摘Nowadays,doctors and nutritionists recommend individuals incorporate selenium-rich foods such as nuts,cereals,and mushrooms into their regular diet to maintain fitness and overall health.Selenium nanoparticles(SeNPs)exhibit strong chemopreventive capabilities.The anticipations for SeNPs with enhanced and tunable bioactive activities have led to a keen interest in phytofabrication.In this study,the aqueous extract of Clerodendron phlomidis plant leaves was utilized for the synthesis of SeNPs.In traditional Indian medicine,this plant extract is recognized as a significant anti-diabetic agent.The flavonoids tetrahydroxylflavone,7-hydroxyflavanone,and 6,4’-dimethyl-7-acetoxy-scutellarein present in this plant leaf extract demonstrate excellent anticancer activity.These secondary metabolites exhibit the ability to reduce sodium selenite into SeNPs.At a concentration of 13μg/mL,the synthesized SeNPs effectively inhibited the proliferation of the HepG2 cell line.The results suggest that the SeNPs possess promising anti-cancer potential against liver cancer and can be considered as a therapeutic agent for liver cancer treatment.Additionally,the cell cycle arrest induced by SeNPs was further confirmed by the fluorescence-activated cell sorting(FACS)method,indicating that SeNPs could efficiently differentiate cancer cells from normal cells.Notably,it showed a significant improvement in diethylnitrosamine(DEN)-induced Swiss Wistar rat groups.This scientific investigation highlights the high anti-cancer potential of SeNPs,positioning them as a promising therapeutic agent for liver cancer treatment.
文摘The well-distributed, stable selenium nanoparticles (10 nm) with good adhesive ability and biocompatibility were successfully synthesized by using the template of chitosan cross-linked with glutaradehyde. The resulting selenium nanoparticles were used as a new carrier for horseradish peroxidase to construct H2O2 biosensors with good performances.
文摘In this study,sesame leaf mucilage(SmE)was utilized as a natural reducing and stabilizing agent for the green synthesis of selenium nanoparticles(SeNPs).The resulting SmE/SeNPs were then incorporated with chitosan(CT)to fabricate nanocomposites(CT/SmE/SeNPs)in three ratios:1:2,1:1,and 2:1.The composites were characterized for particle size and morphology using transmission electron microscopy.SmE/SeNPs exhibited a well-dispersed spherical structure with an average particle size of 14.73 nm.Among the formulations,the 1:1 CT:SmE/SeNPs nanocomposite showed the most potent in-vitro antifungal activity against Penicillium commune and Penicillium crustosum,with an average size of 108.65 nm.When applied at 0.1%concentration to soft cheese samples inoculated with Penicillium spp.,this nanocomposite significantly inhibited fungal growth and extended product shelf life.This study presents a novel,green-synthesized antifungal nanocomposite combining chitosan and SeNPs stabilized by sesame leaf mucilage-a byproduct not commonly explored for this purpose.The developed nanocomposite offers a sustainable,effective,and food-safe solution to control fungal spoilage in dairy products,particularly soft cheese.
基金supported by National-Local Joint Engineering Research Center for Development and Utilization of Small Fruits in Cold Regions(2023GCZX002)Student's Innovation Training Program of Heilongjiang Province(S202310224060).
文摘Cranberry polyphenols(CPs)were obtained via ultrasound-assisted extraction and purification on a macroporous resin X-5 column.A green synthesis method of selenium nanoparticles(SeNPs)using CPs as reducing agents was then developed.Two types of SeNPs(CP-SeNPs1 and CP-SeNPs2)were successfully constructed and character-ized.The spherical particles were well-dispersed on the polyphenol templates and the addition of the poly-phenols reduced the aggregation of the nanoparticles.Both CP-SeNPs1 and CP-SeNPs2,with average particle sizes of 85.62±0.11 nm and 107.97±0.12 nm,respectively,demonstrated radical-scavenging activities and protective effects on erythrocyte hemolysis.CP-SeNPs2 possessed more significant antioxidant activity,as evi-denced by its higher radical-scavenging rate and greater enhancement of the erythrocyte antioxidant state compared to those of CP-SeNPs1.This study provides a new application of CPs and confirms their great potential in stabilizing SeNPs.
基金supported by the Young Doctor Research Fund of Yantai University(No.SM22B184).
文摘Dendrobium officinale polysaccharides(DOP),as a natural active ingredient,has exhibited strong immunomodulatory and antioxidant effects.Selenium nanoparticles(SeNPs)also possess various biological activities,but the unstable properties limit their applications.This research focused on modification of SeNPs by DOP to form stable complexes(DOP-SeNPs),and the analysis of structural characterization and gut microbiota-mediated immune regulation mechanisms.The results showed that DOP-SeNPs were prepared through hydrogen bond interaction and presented uniform particle size distribution(average particle size of about 100 nm)and good stability.Animal experiments demonstrated that DOP-SeNPs significantly protected the immune organs in cyclophosphamide-induced immune injury mice.Besides,DOP-SeNPs effectively regulated the gut microbiota diversity including promoting the enrichments of probiotics such as Lactobacillus and Lachnospiraceae NK4Al36 group compared with model group,and improved the lymphocyte subsets proportions through the regulation of metabolic pathways including biosynthesis of various antibiotics,protein digestion and absorption,chemical carcinogenesis-DNA adducts.In conclusion,DOP-SeNPs presented stable structure characteristics and improved the immune functions of immune-injured mice by influencing the gut microbiota metabolism.These results will provide a certain theoretical basis and data support for the applications of DOP and the nano-selenium complex in functional food industry.
基金supported by Guangdong Provincial Key Laboratory of Human Digital Twin[2022B1212010004]Special Funds for Science and Technology of Guangdong Province,China[2020JK18203210]+1 种基金Pazhou Lab[PZL2023ZZ0006]Development Fund of Yiweyi Biological Manufacturing(Jiangmen)Co.,Ltd,China[2023Y1103011].
文摘The controlled release of selenium nanoparticles(SeNPs)is pivotal significance in improving bioavailability and mitigating potential side effects.Three types of SeNPs,Selenium nanoparticles synthesized by chemical method(CSeNPs),extracellular polysaccharide-encapsulated selenium nanoparticles(EPS-SeNP),and selenium nanoparticles synthesized by Lactiplantibacillus plantarum(LacSeNPs),were synthesized in this study using chemical reduction,polysaccharide-assisted,and microbial reduction methods,respectively.The selenium release kinetics of the nanoparticles were investigated in simulated gastrointestinal with five kinetics models.CSeNPs released 67.80% of selenium in simulated gastric fluids(SGF)and,depicting a Brownian motion fraction of 81.0.EPSSeNPs released 77.38% of selenium in SGF while only releasing 16.28% in simulated intestinal fluids(SIF).The release process was primarily affected by changes in the nanoparticle structure after Super Case II Transport in SGF and the zero-order model in SIF.The zero-order model provided the best fit for releasing LacSeNP profiles in the SGF.In SIF,the release mechanism of LacSeNPs was regulated by combination of diffusion and carrier swelling or erosion,leading to non-Fickian diffusion.Therefore,synthetic nanoparticle methods determine their structures and compositions,leading to differences in their release kinetics and release mechanisms.Notably,the release of LacSeNPs in the SGF aligns with the absorption patterns of selenium within the human body.The current study elucidates the potential application advantages of SeNPs synthesized using microbial means as selenium-enriched supplements.
基金supported by the Natural Science Foundation of Shandong Province(No.ZR2024MC131)the National Natural Science Foundation of China(No.32400071)the earmarked fund for the China Agricultural Research System(CARS-47).
文摘Compared with conventional selenium forms,microbially biosynthesis selenium nanoparticles(SeNPs)have garnered considerable interest owing to their superior biocompatibility and bioavailability,holding significant potential for applications in technological and health-related fields.This study establishes a sustainable bio-logical platform using the marine yeast Scheffersomyces spartinae 12SS-9 for efficient SeNP biosynthesis via selenite bioreduction.The strain demonstrated excellent selenite tolerance and efficiently converted it into SeNPs,achieving a maximum yield of 17.30±1.14 mg/g dry cell weight under 1 mmol/L Na_(2)SeO_(3) stress.The biosynthesized SeNPs were spherical,amorphous,and polydisperse,with stability conferred by surface-bound biomolecules.In vitro assays revealed significant concentration-dependent antioxidant activities,including effective scavenging of multiple free radicals and inhibition of lipid peroxidation.Furthermore,S.spartinae-derived SeNPs demonstrated a markedly improved safety profile compared to inorganic selenite in two fish cell lines:the Epinephelus coioides grouper intestinal epithelial cell line(ECGI)and Epithelioma papulosum cyprini cell line(EPC),and provided robust protection against H_(2)O_(2)-induced oxidative stress by restoring antioxidant enzyme activities and reducing intracellular levels of reactive oxygen species(ROS)and malondialdehyde(MDA).This work highlights S.spartinae 12SS-9 as a highly efficient and eco-friendly microbial cell factory for SeNP production,offering a promising,safe,and scalable selenium supplement for aquaculture.Additionally,preliminary insights into the mechanisms underlying selenite bioreduction,SeNP formation,and extracellular release in this marine yeast are proposed.
基金National Natural Science Foundation of China(U24A20765)National Natural Science Foundation of China(82141213)。
文摘Inflammation is a fundamental response process to injury,which is coordinated by various inflammatory mediators,including reactive oxygen species,inflammatory immune cells and cytokines.Excessive inflammatory responses can drive the development of numerous diseases,making modulation of inflammatory responses a crucial strategy in the treatment of inflammatory diseases.Selenium(Se),an essential trace element for human health,plays a crucial role in alleviating severe inflammatory diseases by scavenging free radicals,achieving antioxidant effects and modulating the innate and adaptive immune systems in the inflammatory microenvironment.Among the different forms of Se element,Selenium nanoparticles(SeNPs)stand out for their low toxicity and high utilization rate,offering them unique advantages in the fields of medicine and materials science.In addition,functional modification of SeNPs enhances their biological activities in modulating the process of inflammatory diseases.In this review,the construction of various types of SeNPs are firstly discussed.Then,we review the diagnosing effects of modified SeNPs and highlight their biomedical applications in the treatment of inflammation.Finally,we summarize the limitations in the current research of SeNPs as well as the major challenges in clinical transformation,and anticipate the application prospects of new functionalized SeNPs in the treatment of inflammatory diseases.
基金This work was supported by the Major Program of National Natural Science Foundation of China （No. 21334005）, Major International Joint Research Project （No. 21620102004）, the National Natural Science Foundation of China （Nos. 21574102 and 21274114）, Special National Key Research and Development Program of China （No. 2016YFD0400202）, and New Century Excellent Talents Program of Education Ministry （No. NCET-13-0442）.
文摘Nanotechnologies have been exploited to develop safe and effective medicines and pharmaceuticals.In the present study,a novel functional nanomedicine constructed from a bioactive polysaccharide and selenium nanoparficles （SeNPs） was developed.A highly-branched [3-（1→3）-D-glucan （AF1） with high anti-tumor activity was used to self-assemble hollow nanofibers with an apparent average diameter of 92 nm;Se nanopartides were synthesized via the reduction of sodium selenite.The results of light scattering,transmission electron microscopy,and X-ray diffraction demonstrated that the spherical SeNPs with a mean diameter of 46 nm were entrapped in the cavities of the AF1 hollow nanofibers through the formation of Se-O bonds between SeNPs and AF1,leading to the good dispersion and high stability in water for over 16 months.In vitro and in vivo assays indicated that the AF1-Se nanocomposite had higher anti-tumor activities against breast cancer.Furthermore,AF1-Se displayed a broad-spectrum inhibition against human cancers with low half maximal inhibitory concentration （IC50） values and low toxicity to normal cells.Particularly,the inhibition ratio of AF1-Se against MCF-7 cancer cells reached 75% at a concentration of 200 μg.mL-1 with 29 μM Se content,much higher than that by treatment with AF1 alone,suggesting a strong synergic effect and nano impact.Overall,we developed a method for increasing the stability,anti-tumor activity,and safety of SeNPs by wrapping with bioactive polysaccharides.
基金supported by the fund from the Open Project of Guangdong Key Laboratory of Marine Materia(LMM2020-7)the technology planning projects of Guangzhou(202201020655 and 202102010202)+2 种基金the Guangdong Natural Science Foundation(2020A1515110648)the Open Fund of Guangdong Provincial Key Laboratory of Functional Supramolecular Coordination Materials and Applications(2020A03)the Guangzhou Medical University Students’Science and Technology Innovation Project(2023ekky009 and 02-408-2203-2079).
文摘Human adenovirus(HAdV)can cause severe respiratory infections in immunocompromised patients,but its clinical treatment is seriously limited by side effects of drugs such as poor efficacy,low bioavailability and severe nephrotoxicity.Trace element selenium(Se)has been found will affect the disease progression of pneumonia,but its antivirus efficacy could be improved by speciation optimization.Therefore,herein we performed anti-HAdV effects of different Se speciation and found that lentinan(LNT)-decorated selenium nanoparticles(SeNPs)exhibited low cytotoxicity and excellent anti-HAdV antiviral activity.Furthermore,SeNPs@LNT reduced the HAdV infection-induced mitochondrial damage and excessive production of reactive oxygen species(ROS).It was also involved in the repair of host cell DNA damage and inhibition of viral DNA replication.SeNPs@LNT inhibited HAdV-induced apoptosis mainly by modulating the p53/Bcl-2 apoptosis signaling pathway.In vivo,SeNPs@LNT replenished Se by targeting the infected site through the circulatory system and was involved in the synthesis of Glutathione peroxidase 1(GPx1).More importantly,GPx1 played an antioxidant and immunomodulatory role in alleviating HAdV-induced inflammatory cytokine storm and alleviating adenovirus pneumonia in Se-deficient mice.Collectively,this study provides a Se speciation of SeNPs@LNT with anti-HAdV activity,and demonstrate that SeNPs@LNT is a promising pharmaceutical candidate for the treatment of HAdV.
