The production of selenium-enriched fish can contribute to alleviating selenium deficiency in human diets.However,it is still unclear which selenium source,as an additive,can efficiently and costeffectively produce hi...The production of selenium-enriched fish can contribute to alleviating selenium deficiency in human diets.However,it is still unclear which selenium source,as an additive,can efficiently and costeffectively produce high-quality selenium-enriched fish.This study evaluated the effects of selenium nanoparticles(SeNP),selenite,and selenomethionine(SeMet)on the growth,antioxidant capacity,selenium content,selenium speciation,and meat quality of grass carp.Ten diets were prepared,including a basal diet(BD)and three concentrations(0.1,0.3,and 0.9 mg/kg)of SeNP,selenite,and SeMet.A total of600 fish(250.79±1.57 g)were randomly assigned to 30 tanks(3 tanks/group).Fish were fed the experimental diet three times daily for 60 d.In this study,SeNP most significantly promoted the growth and antioxidant capacity of grass carp,with 0.3 mg/kg SeNP identified as the optimal additive concentration.Additionally,SeNP demonstrated equally excellent bioavailability as SeMet and significantly increased the content of SeMet in grass carp(Ctenopharyngodon idella)muscle.Furthermore,compared to SeMet and selenite,dietary SeNP could more significantly enhance the content of selenocysteine(SeCys2)and methylselenocysteine(MeSeCys)in grass carp muscle tissue.In addition,we have demonstrated that SeCys2and MeSeCys promote apoptosis of cancer cells(HeLa)through the mitochondrial apoptotic pathway(involving Bax and Bcl-2).Furthermore,as an additive,0.3 mg/kg SeNP significantly improved the flesh quality of grass carp by reducing crude fat and heavy metal content,as well as increasing the levels of eicosapentaenoic acid(EPA)and docosahexaenoic acid(DHA)and the ratio of n-3/n-6 polyunsaturated fatty acid(PUFA).In summary,SeNP is the most suitable additive for producing selenium-enriched fish.展开更多
Selenium nanoparticles synthesized by Lactobacillus(LacSeNPs)are considered the safer form of selenium supplementation.This study synthesized a new LacSeNPs by a selenium-tolerant strain Lactiplanibacillus plantarum B...Selenium nanoparticles synthesized by Lactobacillus(LacSeNPs)are considered the safer form of selenium supplementation.This study synthesized a new LacSeNPs by a selenium-tolerant strain Lactiplanibacillus plantarum BSe,and investigate its characteristics,antibacterial activity,and cytotoxicity.The results showed that L.plantarum BSe could grow at Se(IV)concentrations of 100 mmol L^(-1),and achieving a conversion level of 76.32%of selenium into LacSeNPs.Electron microscopy,FT-IR and X-ray photoelectron spectroscopic data indicated that only elemental Se existed in LacSeNPs and was encapsulated by proteins and polysaccharides,and the atomic proportion was Se(24.83%),accompanied with C(67.03%),N(3.12%),O(4.24%)and S(0.78%).LacSeNPs showed strong inhibition to Escherichia coli and Staphylococcus aureus.Furthermore,at equivalent selenium concentrations,LacSeNPs exhibits lower cytotoxicity compared to selenite.The findings of this study demonstrate that Lactiplanibacillus plantarum BSe is an excellent LacSeNPs synthesis bacterium and has potential application value in the production of SeNPs food additives.展开更多
基金supported by the Key R&D Plan Project of Shaanxi Province(S2022-YF-YBNY-0055)the Research and Development Program of China Se-enriched Industry Research Institute(No.2021FXZX04-01)the Fund of Key Sci-Tech Project of Shaanxi Province(NO.2018ZDXM-NY-034)
文摘The production of selenium-enriched fish can contribute to alleviating selenium deficiency in human diets.However,it is still unclear which selenium source,as an additive,can efficiently and costeffectively produce high-quality selenium-enriched fish.This study evaluated the effects of selenium nanoparticles(SeNP),selenite,and selenomethionine(SeMet)on the growth,antioxidant capacity,selenium content,selenium speciation,and meat quality of grass carp.Ten diets were prepared,including a basal diet(BD)and three concentrations(0.1,0.3,and 0.9 mg/kg)of SeNP,selenite,and SeMet.A total of600 fish(250.79±1.57 g)were randomly assigned to 30 tanks(3 tanks/group).Fish were fed the experimental diet three times daily for 60 d.In this study,SeNP most significantly promoted the growth and antioxidant capacity of grass carp,with 0.3 mg/kg SeNP identified as the optimal additive concentration.Additionally,SeNP demonstrated equally excellent bioavailability as SeMet and significantly increased the content of SeMet in grass carp(Ctenopharyngodon idella)muscle.Furthermore,compared to SeMet and selenite,dietary SeNP could more significantly enhance the content of selenocysteine(SeCys2)and methylselenocysteine(MeSeCys)in grass carp muscle tissue.In addition,we have demonstrated that SeCys2and MeSeCys promote apoptosis of cancer cells(HeLa)through the mitochondrial apoptotic pathway(involving Bax and Bcl-2).Furthermore,as an additive,0.3 mg/kg SeNP significantly improved the flesh quality of grass carp by reducing crude fat and heavy metal content,as well as increasing the levels of eicosapentaenoic acid(EPA)and docosahexaenoic acid(DHA)and the ratio of n-3/n-6 polyunsaturated fatty acid(PUFA).In summary,SeNP is the most suitable additive for producing selenium-enriched fish.
基金supported by Guangdong Key R&D Program,Department of Science and Technology of Guangdong Province,China[2018B020205002]Guangdong special funds for science and technology innovation strategy,China[2021JK02402003&2018JK35202003].
文摘Selenium nanoparticles synthesized by Lactobacillus(LacSeNPs)are considered the safer form of selenium supplementation.This study synthesized a new LacSeNPs by a selenium-tolerant strain Lactiplanibacillus plantarum BSe,and investigate its characteristics,antibacterial activity,and cytotoxicity.The results showed that L.plantarum BSe could grow at Se(IV)concentrations of 100 mmol L^(-1),and achieving a conversion level of 76.32%of selenium into LacSeNPs.Electron microscopy,FT-IR and X-ray photoelectron spectroscopic data indicated that only elemental Se existed in LacSeNPs and was encapsulated by proteins and polysaccharides,and the atomic proportion was Se(24.83%),accompanied with C(67.03%),N(3.12%),O(4.24%)and S(0.78%).LacSeNPs showed strong inhibition to Escherichia coli and Staphylococcus aureus.Furthermore,at equivalent selenium concentrations,LacSeNPs exhibits lower cytotoxicity compared to selenite.The findings of this study demonstrate that Lactiplanibacillus plantarum BSe is an excellent LacSeNPs synthesis bacterium and has potential application value in the production of SeNPs food additives.
